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HomeMy WebLinkAboutSUS343r DEP"RTHENTOF FISH .. ~NDGilME February 17. 1982 Mr. Eric Yould Executive Director Alaska Power Authority Anchorage, Alaska (lear Mr. Yould: RECEIVED ALASKA POWS\ AUTH0;11"fY The Alaska Department of F" h and Game submits the following program and budget proposal (Attachment A 'fhrough F) to the Alaska Power Authority (APA) as review draft of our reconmendations for the Phase !! FY83 Aquatic Study. Attachment A presents the xt of our present June 1981 RSA agreement with recommended changes in br cketed anc underlined text immediately following the original text to be c anged and which is so underlin1rl. We request that APA rev ew this draft as soon with Robert· Mohn and ave Wozniak be arrang development at their ea liest convenience. Sincerely, Thomas W. Trent Su Hydro Aquatic St dies Coordinator cc: R; Andrews S. Pennoyer 0 L s poss i b 1 e and a meeting to discuss its further , ==============================::;;,; ,~ DRAFi ATTACHMENT A -GENERAL TERMS AND ACCORD OF AGREEMENT The Alaska Power Authority and the Alaska Department of Fish and Game have mutually agreed: 1. 2. 3. that the fish and wildlife studies are necessary effort to detPrmine potential impacts of the Susitna Hydroelectric Project on the valuable fish and wildlifE resources of the Susitna River Basin. that Acres-American, the prime contractor for the Susitna Hydroelectric Project Feasibility Studies, will review the results of ADF&G's field programs providing baseline fish and wildlife population and habitat information, and as the Alaska Power Authorities repre- sentative prepare the fish and wildlife Exhibit~ (E), of the filing documents for the Federal Energy Regulatory Commission license for. the project. that, although the studies conducted in Phase I of the Susitna Hydroelectric Feasibility Studies can provide a preliminary assessment of project impacts and are a basis for preparation for the Exhibit~ (E), continuation studies into Phase II will b~ essential to make the best judgement of the project impacts and identify fish and wildlife mitigation alternatives. A-1 L ,~ L r: L r L~ r· I L~ [ [ L L -, ORAFi Therefore, the Alaska Power Authority has agreed to fund the Alaska Department of Fish and Game participation in the Susitna Hydro Feasibility studies. and AOF&G agrees to implement these st!_dies (the aQuatic studi~s) as follows: ADF&G Studies Susitna Hydro Feasibility Study Team and its General Functions 1. 2. The Alaska Department of Fish and Game will establish a Susitna Hydro F~asibility Studies Team. The ADF&G Susitna Hydro Feasibility Studies Team will function in the: a. coordination and further development of the fish and wildlife studies with the APA, Acres-American, other fish and wildlife or resources agencies, and other feasibility study contractors as appropriate b. the development and recommendation of the Department's policies, concerns, and advic~s with respect to resource protection, study direction and their progress to APA, Acres-American, other fish and wildlife and resource agencies and study contractors. A-2 DRAFI c. representation of the Alaska Department of Fish and Game on the Fish and Wildlife Steering Committee. and for coordinating the involvement of other delegated Department representatives from outside the study team. d. review and approval. as delegated by the Commissioner to the ADF&G Studies Coordinator. of the Susitna Hydro Project Feasibility Study activities of APA, Acres-American or their subcontractors which may affect State designated anadromous fish waters. (This sectioA should be deleted) e. implementation of the fisheries baseline studies. (Wildlife studies are covered in a separate agreement, but staff of these studies are part of the ADF&G Studies Team.) Administration and Support APA has agreed: 1. To fund ADF&G administration and support services for ov~rall Susitna Hydro Project Studies coordination, A-3 r~ L r L [ [ [ [ [ [ • • DRAFT planning and implementation as shown in Attachment I (for Phase 11 as shown in Attachment I.). The purpose of these funds is to: a. provide for basic State personnel and budget administration at the Anchorage office of the Study Team, and additionally fund administrative support required from within the ADF&& Division of Administration in Juneau. b. provide for support services of: 1) ADF&G personnel to develop, manage, and analyze data being generated by the fisheries field program 2) 3) ADF&G personnel to maintain and construct equipment apparatus needed for the field program ADF&G personnel to edit scientific and technical reports and documents generated by the field research program A-4 • 2. • • DRAFT That Acres-American provide certain supplemental administration and support services directly to ADF&G as shown in Attachment II. The purpose of this support is: a) provide clerical assistance for typing of required reports and documents r~lated to the Susitna Hydro Project, (until September 30, 1982). b) provide cartographic and drafting services for required technical and scientific reports and documents (until September 30, 1982). c) provide the full time assistance of a hydraulic engineer to the ADF&G to aid in planning, im.plementation and evaluation of seasonal and spatial habitat (aquatic habitat and instream flow) studies in consultation with ADF&G, the Alaska Department of Natural Resourtes and the U.S. Fish and Wildlife Service (until September 30. 1982) (~) (~fter September 30, 1982 thote positi~ns required f~r support under (a) and (b) above will be funded as state positi~ns within the Department of Fish and Game.) A-5 :- [, [ [ [ r L [ [ [ [ L [ L L .. ., • -' I DRAFT (!) (After September 30, 1982 the servjces of a hydraulic engineer will be funded through an appropriate contract of the APA with the Phase II studies prime contractor.) d) (!) provide office space, warehousing, workshop and outside storage space to support the fisheries field studies, and the program administrative, coordination and support staff, as well as the office supplies, office equipment and communication services required for this staff (until September 30, 1982) . (After this date funds and support of these services !.!ll provided to and managed by the Alaska Depart- ment of Fish and Game.) A-6 • • DRAFT ADF&G agree!: that the Clerk IV (Administrative Assistant !} under the Su-Hydro Coordinator, in addition to other duties, will be responsible for the coordination, on a departmental basis, of the monitoring and administrative processing of all personnel, purchasing. and accounting documents for the Division of Sport Fish, Division of Commercial Fisheries, and Habitat Protection Section (delete the preceeding). The Divisions of Sport Fish and Commercial Fisheries will forward all approved personnel, purchasing, and accounting documents directly to the Su-Hydro Coordinator's Office to be processed for payment~ etc. The Divisions of Sport fish and Commercial Fisheries will provide to the Su-Hydro Coordinator's Office, each month, r.opies of the payroll warrant registers, monthly expenditure journals (MEJs), and current year authorization balances runs for their codes affected by the APA project. The Clerk IV (Administrative Assistant!). in turn, will provide to the Divisions of Sport Fish and Commercial Fisheries monthly audit reports on the status of their fund balances. Field Program • ADF&G agrees to implement the fisheries-aquatic studies program as shown in Attachment ill· (Attachments B through ) A-7 [ [ [ [ [ [ [ [ [ [ [ [ [ [ [ [ [ [' "-" <~-·---~-L ] _j j j • j j ORAFi APA and Acres-Amer1can have agreed that Acres-American will provide the equipment shown in Attachment !! (Attachments B through F ) for use in the ADF&G fisheries program. { U"nt i 1 September 30, 1982 and APA designates the contractor who will continue this function after this date). The schedule for obtaining and the release and use of this equipment to ADF&G by Acres-American will be in accordance with the field study timeframes shown in Attachment III, {Attachments 0 throuih_f) • . Helicopter Support APA and Acres-American have agreed that Acres-American will provide helicopter support for transport of field crews, equipment and material in the studies area. The minimum air hour requirements for helicopter support will basically follow these outlined in AOF&G's October 11· 1979 (February 1982) study proposals and modified to the timeframes below: (delete the preceding) Resident & Juvenile Anadromous Project Aquatic Habitat and Instream Flow Project Adult Anadromous Project A-8 Phase II FY 83 120 hrs. 117 hrs. 30 hrs." 267 hrs. Reporting Reouirements of reporting requirements to Acres-American and Terrestrial Environmental Services as shown in Attachment V. DRAFT (The Alaska Deoartment of Fish and Game will provide draft annual report on data collection by February 1 a final draft by March 1 of each project year 1 commenc with FY 83. ADF&G will provide budgetary requirements for an impact analysis of thes~ data collection reports if APA requests the ADF&G to assist with and/or perform this function. This report would be due by May 1 of each project year.) Procedures Manual The Alaska Department of Fish and Game will provide procedures manual sections to Acres-American and Terrestrial Environmental Services in accordance with Attachment VI. (The Alaska Department of Fish and Game will provide an annual update of the aquatic studies procedure manual by June 1 of each p.roject year.) A-9 [ lc [ [ [ [ [ [ [ r~ 1_.; [ [ L L A TT ACH11ENT A-1 SU HYDRO AQUATIC STUDIES FY-83 BUDGET Sm·vtARY Sport Fish Conmercial Fish Line line - 100 1,848.5 100 485.3 200 91.1 zoo 17.9 300 461.2 300 91.4 400 233.7 400 122.5 Subtotal 2,634.5 Subtotal 717.1 ADF&G Equipment Supplied by Acres American, Inc. Sport Fish line 500 Subtotal 342.3 342.3 Conmercia·l Fish line 50\J 59.5 Subtotal 59.5 Total 3,351.6 Total 401.8 Subtotal 3,753.4 SF -CF Support Services Supplied by Acres American, Inc. until September 30, 1982 line 100 300 400 Subtotal 34.1 39.2 1.8 75.1 Total 75.1 * Grand Total this Draft 3,828.5 * Includes stock separation costs but excludes Watana Camp and helicopter support costs supplied by Acres American, Inc. Personal services (line 100) do not include FY-83 salary increases appropriated by legislative action. A-10 • • • DRAFT ATTACHMENT B -ADMlNISTRATlON AND SUPPORT UNIT The Alaska Department of Fish and Game Su Hydro Aquatic Studies is composed of five organizational elements. these are: 1. Administrative and Support Unit 2. Data Processing Unit 3. Resident and Juvenile Anadromous Project 4. Aquatic Habitat and Instream Flow Project 5. Anadromous Adult Project This program statement outlines the basic objectives for each of these program elements. Tasks to be accomplished within each element. the manpower requirements and budget to accomplish these tasks for FY 83 are in the attachment sections of the program. ADMINISTRATION and SUPPORT UNIT Background The Administrative and Support Unit of the Su Hydro Aquatic Studies is composed of staff administering and directing the overall staff and activities of the other four project [ [ [ [ [ [ [ [ ... [ [ [ [ [ [ [ [ [ [ -----------L DRAFT elements and of staff who provide clerical, budget and expenditure, equipment purchase, and repair services for the project. This unit is responsible through the Aquatic Studies Coordinator, for coordination with APA, other dgencies and contractors on administrative and technical matters, and also at the Direction of the Commissioner of the Department of Fish and Game and Directors of the Sport Fish ~~~~~~--~~~~~~~~~~~~~~~~~~~~~~ and Commercial Fish Divisions carries out those duties assigned as necessary to accomplish and report on the Su Hydro Aquatic Studies as a office of the Department of Fish and Game. 8-Z • • DRAFT Objectives 1. To function, in FY-83, in a coordination role between the other four organizational elements of the Su Hydro Aquatic Studies Team, othe·r Department Divisions and: a) the Alaska Power Authority (APA); b) sub-contractors of the APA; c) Federal, state and local government agencies; d) private corporations; e) special interest groups; and f) the public on matters related tc the Alaska Department of Fish and Game aquatic studies data collection program being conducted for the proposed Susitna Hydroelectric Project. [ [ [ L r~ L~ [ [ [ [ [ [ [ [ [ r~ I [__; L [ L D ') ""-~~-~~--~[ DRAFT Taskt to be pe¥foreed under this objective are: a. to plan and coordinate the develQpment of the fisheries, aquatic habitat and instream flow studies by the Su Hydro Aquatic Studies Team with the APA and its contractors, governmental agencies,. private corporations, special interest groups, and the public as appropriate. b. to provide support for the development of ADF&G policy with respect to the Departments concerns, advices, and recommendations regarding fisheries resource and aquatic habitat protection to the APA and its contractors, governmental agencies, private corporations, special interest groups, and the public. c. to represent the Department of fish and Game on the Su Hydro Steering Committee and the Su Hydro fisheries~itigation Task force Core Group,. and coordinate the involvement of other Departmental representatives as required on these committees. • • • DRAFT 2. To provide and coordinate administrative, personnel and equipment support services to the other four project elements in FY 83. Tasks under this objective are: a. to provide for the general administration of the collective ADF&G Su Hydro Aquatic Studies Office. b. to provide basic State personnel and budget administration for the Su Hydro Aquatic Studies Team. c. to coordinate and direct the work of the support personnel employed by Acres American for the ADF&G Su Hydro Aquatic Studies Team, until September 30, 1982. d. to administer the purchase of capital equipment pL~chased through Acres-American for the Su Hydro Aquatic Studies on other contractors as designated by APA. [ [ [ [ [ [ [ [ [ [ [ [ [ [ f' L-' [ [ e. to administer, and provide for the L ~'----------------[ .. .. '1 3 ..,_ e- ., "'I ' j --., J --, ' --' 'l j 1 ~ ' j --, .. -.., __. f. DRAFT maintenance and repair of capital equipment used by the Su Hydro Aquatic Studies Team. to review and edit as drafts of scientific and technical reports. B-6 ATTACHMENT B-1 ADMINISTRATION AND SUPPORT UN]T Line 100 -Personal Services PCN 4188 4220 4187 4222 *NEW *NEW 4194 4192 1721 4189 4185 Class Grade FB V F&G Supervisor 22 FB III Asst. Proj. Coord. 18 81ometrictan III 18 Adininis-trative Asst. I 12 Clerk Typist III . 8 Clerk Typist III 8 FB III RJ 18 FB. Ill AH 18 FB-III AA 18 Maintenance r~ech. II Mechanic IV Line 200 -Travel Project Coordinator's Office Project Leaders Equipment Maintenance Line 300 -Contractual Services Project Coordinator's Office Project Leaders Equipment Maintenance Line 400 -Commodities Project Coordinator's Office Project Leaders Equipment Maintenance P/S permanent seasonal .... - 12 12 12 12 9 9 12 12 12 12 12 P/S Grand Total (Line 100 -400) Cost ,~ ;L 63.8 ~ )~ $0.7 53.6 29.6 18.8 18.8 5L9 ", 52.9 -~·-~ . .) 57.8 45.7 .34.2 477.8 23.0 16.6 4.0 43.6 ; 147.8 , 37.9 1.0 192.7 8 .. 6 5.5 17.0 31.1 74';.2 I . ___ _j r . • • • ATTACHMENT 8-2 ADMINISTRATIVE SUPPORT UNIT line 500 -Equipment to be purchased through Acres American.. Inc. [ [ [ I~em Quantitx Description 1 12 Desks 2 5 Book cases 3 6 File cabinets 4 12 Chairs, desk 5 2 Chair posture 6 1 Drafting table. 72 .. 1 i Light table 8 2 Drafting stool 9 Drafting tools 10 1 U1 trasonic pen cleaner 11 1 Drafting tool cabinet 12 1 Tape recorder 13 1 Slide project or w/access. 14 2 Typing Station desk R-8 Ur,tft Cost 0 .. 3 0.2 0.2 0.2 0.3 0.5 0.7 0.2 0.3 0.4 0.5 0.3 Total Cost 3.6 1.0 1.2 2.4 0.6 0.5 0.7 0.3 0.6 0.2 0.3 0.4 0.5 0.6 12.9 r L ,~ L [ [ [ [ [ [ r L [ [ L " ----"-----[ 4it ATTACHMENT 8-3 EQUIPMENT MAINTENANCE -ADMINISTRATIVE SUPPORT UrHT line 500 -Equipment to be purchased thro.ugh Acres American, Inc. Item Quantit,l Description Unit Cost To.tal Cost 1 1 Tool set 2/cabinet 2.0 2.0 2 1 Sh~etmetal Break~ 52Q 1.0 1.0 ~, ~ ..J 3 1 Floor jack 0.3 0.3 .., 4 N/A Special 08 jet tools 0.6 0.6 ~ 5 N/A Special 08 tools 0.6 0.6 ., • 6 N/A Tool Replacements 5.0 5.0 ..J 7 N/A Metal parts bins 2.0 2.0 11.5 j ' • ILQ ·---'-· • :;» .. ~ r-------- Biometrician II PCN 4187 • Aquatic S'tud1es Coordinator FB "IV PCN 1188 I Assistant Coordinator FB Ill PCN 4220 I Ha1ntenancer;upervhor Off1;l,anager MMII AAI PCN l4189 PCNI 4222 MAintenance Asshhnt I I • M IV Acres Typist Aeres Typtst PCN 4185 i -:c -"' .... ~ -< "" "' c "0 C! = i -.... r------------ Aqutt1c tlab1tat Project Lead•r FB Ill ----------r--------------· -------------.---------1 Resident/ Anadromous1 Juven 11 e Adu 1 t Anadromous PCN 4192 lJ r-J r: r-J r-. -J r-J r: Project Leader Project Leader FB Ill FB 111 PCN 4194 PCN 1721 L"l r:l r-J r; L:l r--; ,_~ L. • r-:l LJ li ~ 11 ~ ~ -.... "" a ell' I ... ~ ...... ' J j Jl DRAFT ATTA£HMERT C -DATA PROCESSING UNIT Background The extensive studies ~eing cartied out by the ADF&G•s Su Hydro Aquatic Studies Team entail the collection of Jarge quantities of field data concer~ed with the distribution and abundance of fish, and the aquatic habitat associated with these fish in the Susitna River drat nage. The data processing support unit &f the study team was created to provide the expertise and •manpower• necessary to catalog, summarize, and analyze these data. The cataloguing of this field data into computer data bases is a necessary precursor to easy retrieval, analysis, and summarization of the field data. Summal"izati on of the 1 a rge quantities of cc.llected field data into a concise and understandable form requires the capabili~ies of a digital computer with data base management and report production capabilities. The use of a computer to help in this summarization ensures that errors in data ~eductibn and analysis are kept to a minimum. lhe use of computers requires the expertise of a computer programmer and other associated electronic data proce~sing personnel. ~-1 • • DRAFT An additional facet of the data processing unit fs its involvement by the untt•s bio-metrician in statistical analysis of field data and experimental sampling design of field studies. Experimental sampling design of field studies is a necessary step in any scientific investfgat1on. The natural variability of the "real world" results in variability of field measurement! resulting from a field study often appear conflicting antl nonsensical. However, with a statistically designed experimenta· sampling procedure the resultant measurements can be depended upon to provide the best possible answer to the study•! o~jectives. Statistical analysis of field data is necessary due to the inherent va ri abi 1 i ty of field data, for example the number of fish collected by a fish trap would vary from sample to samp.e even if the abundance Gf the fish in the natura 1 popu 1 ati on remained constant. On the other hand the abundance of the fish population is expected to vary between one sampling period and the next period. Accordingly. statistical analysis is necessary tG ensure that observed variations in field measureme1ts (e.g. numbers of fish collected) are large and accurate enough to indicate an actual variation in the cbaracte~Jri5tic of interest. Note that the same can be said for the opposite condition. that f~, ~ ---· [ r-, ~ r-, [ [ [ [ [ [ [ [ [ [ [ [ [ [ ------__;,.___~ L • DRAFT is, lack of variations in field measurements must also be analyzed statistically in order to ensure that the observed stability of the measurements indicates an actual stability of the characteristic of interest. The data processing support urtit of the aquatic study team also provides biometric evaluation of scientific reports. This evaluation is designed to ensure that all analyses. interpretations. and cone 1 us ions in these reports are founded upon observed fie 1 d data. Furthermore.. the eva 1 uat ion should make sure that all conjectures and hypotheses in these reports are iabeied as such. Objectives The objectives of the data processing support unit of the ADF&G Su Hydro Aquatic Studies Team in FY 83 are as follows: 1. To provide data processing support to the three field program elements. The tasks under this objective are: a. r.ornputerization of all data collected in the field studies. This computerization into DRAFT retrievable data bases allows for easy and accurate retrieval of data for analysis and summarization; b. production of summary report tables and computer plotted graphics, with simple univariate statistics (e.g. minimums, maximums, means, and confidence intervals); and c. programming and execution of programs to provide computer quality execution of statistical analyses. 2. To provide statistical analysis of scientific and technical data collected by t~e field program elements. The tasks under this objective are: a. computation of fish population estimates and associated confidence intervals from mark/recapture field studies and sonar counts; b. comparison of biological attributes of fish populations (e.g. length, weight, and age) among sampling areas and sampling periods; [~ [ :L [ [-· [ [ [ [ [ [ [ [ [ [ [ [ [ ~---------[ -' DRAFT c. testing for possible relationships among aquatic habitat parameters• and d. testing for possible relationships among aquatic habitat parameters and the associated fish populations in sampled areas. 3. To provide advice on experimental sampling design of the field study programs, to ensure that the field studies will yield the type and quantity of data , It needed to meet their objectives. 4. To provide biometric evaluation of the study team•s scientific reports, to ensure that all conclusions are founded upon sound statistical analysis and interpretation of the collected field data. Work Plan The proposed work plan for meeting the objectives of the data processing support unit is be. sed pri rna ri ly on the procedures develop in FY 1982. During FY 1982, data collected from the winter o~ 1980 through the fall of 1981 was catalogued, C-5 • DRAFT su.-arized, and analyzed. This process entailed starting up the data processing unit in teras of personnel. and computer hardware and software. The procedures develop during this time are outlined in the following discussion. Computerization of field data entails coding of data onto keypunching coding sheets by data processing pe~sonnel. During the coding process initial checks are made for accuracy of recorded field data in terms of clarity. consistency, and feasibility {e.g. can the water temperature really be 40C?!!?). Coded keypunch forms are then sent to Boeing Computer Services {BCS) for keypunching. We have found the BCS keypunching services to be a very high quality and reasonable price. After completion of keypunching, the data is loaded into the BCS computer via cards and then processed through an error searching program called DATAMAP. DATAMAP can find gross errors due to miscoding and keypunching. These gross errors are corrected then the data is input into a data base management system call SIR . 1Scientific Information Retrieval). SIR provides data processing with the capability of extensive data base management tools such as sorting. conditional retrieval~ simple statistics, and interfacing with more advanced computerized statistical packages (i.e. SPSS and BMDP). [ [ [ [ r~ [ [ [ [ [ [ [ [ [ [ [ [ [ l I a ' DRAFT An indispensable aid in the process of computerization has been the computing capabilities provided by an inhouse microcomputer. The microcomputer is a Vector Graphics 3005 system. with 56K memory. Z-80A 11icroprocessor, 5.25 inch floppy disk drive, 5.25 inch hard disk drive with 5 megabytes of on-line storage, and a letter quality printer. The mfcro has been used for interaction with the BCS mainframe computer, this interaction is of a complexity and speed which could not be attained by an ordinary •dumb" terminal. Report quality tables have been produced with the microcomputer and its associated printer. An additional interface with a Tektronix 4662 flat-bed plotter provides data processing with the capability to produce report quality graphic presentation of field data. A Tektronix 4010 graphics terminal will shortly (March-April 1982) be useful in utilizing the BCS's full range of graphics packages, which will free up inhouse programming efforts for other tasks. During 1981 the tasks of data processing were carried out by a Biometrician II, an EDP Programmer III, and a Data Control Clerk II. These tasks were primarily directed at the first two objectives listed above (i.e. data processing support and statistical analyses)., very limited experimental C-7 _ • • • DRAFT design or biometric evaluation was carried out itt 1981. The lack of input into experimenta 1 design in 1981 was primarily due to the late hiring of the project biometrician. However the shortage of biometric input and evaluatian of project reports was due to a heavy workload of data processing and programming activities of the project biometrician. Computer programming activities by the biometrician were necessitated due to the initial start up nature o.f data processing (accordingly more programming was necessary in 1981 than is expected in 1982), there will still be some programming carried out by the biometrician in FY 1983. If the FY 1983 staff remains the same then a good deal of the data processing activities of the bfometrican would be expected to be mare time consuming in FY 1983, due to proposed increased fie 1 d co 11 ect ian efforts and the taking over of more data processing activities that were carried out by Adult Anadromous personnel in FY 1982. Accordingly, we are proposing a new staff position for FY 83. ·' ~-· ~' [ [ r -, ~" [ [ [ [ [ [ r' [ [ L [ [ The new position is a Fishery Biologist I/II. We are requesting a fishery biologist (rather than a programmer or a data control clerk) due to the need for someone in data processing with experience in field data collection techniques and biological conditions. that is someone who could readily recognize and correct •problems• with field L ______.;__,;___ [ C-8 • . , i • ~ "' jj -' • DRAFT data. Presently. this recognition and correctiott of problems is carried out by the biometrician and field biologist in the three field projf!cts. Additional duties of the fishery biologist would be to coordinate data processing activities such as collecting data from the three field projects (this would sometimes entail field work). coordinate and help in coding of data onto keypunch coding forms. and coordinate report production and analyses on the computer . The coordination of report production and computer analyses would be under supervision of the biometrician. but standard report production attivftfes could and would be coordinated by the fishery biologis~. The time freed by the taking up of duties by the new data processing fishery biologist would allow the staff biometrician more time for the last two data processing objectives listed above. The staff data control clerk would have enough time to do most of the coding and error checking of the data • Finallyt the staff programmer would be freed from data processing activities and could devote full time to programming. It is still anticipated that some temporary help will be needed for some coding and error checking of data. a • • DRAFT Accordingly. the budget includes nine months of fishery biologist I wages to be used for •picking up• seasonal personnel after the end of the ice-free field season. The budget for travel, commodities, and capital purchases is designed to provide adequate funds for all of data processing•s task. Some of the funds outlined are items necessary to support a new fishery biologist position. The proposed data processing budget does not include administrative and support funds for the new fishery biologist position (e.g. desk and moving cost). Most of the new equipment and funds are necessary to a 11 ow the staff biometrician and the programmer with adequate tools to do their job. The two new microcomputers requested (alo~g with the semi-disk and software packages) are necessary in order to allow both the biometrician and programmer to do accomplish their assigned tasks in an efficient manner, while still providing access of the present microcomputer to the data control clerk and the new fishery biologist. C-10 l' [ [ [ [ [ [ [ [ [ [ [ [ [ [ [ [ [ [ I ATTACHMENT C-1 DATA PROCESSlNC UNIT line 100 -Personal Services Class Grade 4193 £0P ProgrUIIIer III 17 4191 Data Control Clerk II 11 *NEW FB I/II 16 4190 Cartog.raph~r II 15 BORROSIED FB I 14 line 200 -Travel Travel & Per"Diem line 300 -Contractual Services Boeing Computer Services Instream Flow Analysis Training Other line 400 -Commodities Data Processing Related Supplies Drafting Supplies C-11 "' 12 12 12 9 9 Cost 46.8 31.8 42.0 28.1 29.7 Subtotal li!:l 2.4 Subtota 1 -r.4 Subtotal 6.8 1.0 Subtota 1 /:f Grand Total (line 100 -400) 295.1 ·~ [ [ [ [ [ [ [ r L [ i [ • [ [ [ [ [ [ ,[ l[ --~~[ ATTACHMENT C-Z DATA PROCESSING UNIT Line 500 -Equipment to be purchased through Acres American. Inc. It~m Quantity Qescription Unit Cost Total Cost " 1 z Vector Graphic microcomputer 9.0 18.0 ., 2 3 Semidisk, disk emulators 2.3 6.9 -' 3 4 A/8 switch boxes w/cables 0.3 1.2 ... ~ 4 2 Microcomputer terminal tables 0.3 0.6 .. 5 1 VA3451 triple Modem 1.0 1.0 I 6 1 Oatapod reader 1.8 1.8 _,; .., 7 1 Datapod storage eraser o.z 0.2 _j 8 4 Software packages 1.9 9 2 Hewlett-Packard 11-C calculators 0.2 0.4 10 Parts in excess of $100.00 not covered under warranties 3 .. 0 -" 35.0 ~ ... .i.i " .1i ~ _. I' C-12 L' n I .... w lJ • Acres Cartographer until 30 September. 1982 Cartographer 1 I PCN 4190 r-----1 l J c---': r~ rJ FB 1/11 NEW PCN Aiiiilii.: .., Data Processing Supervisor 81ometric.1an I I PCN 4187 EDP Prograrrmer III PCN 4193 r-l r-J ~ ~ r-l r---' rJ ,., Data Control Clerk 11 PCN 4191 LJ r-----1 l ' c-J :----' ,·~~ ! ~ )It ~ ~ ! ,.... ...,. ~ tn n .... . z w en i ~ ~ lJ • , DRAFT ATTACHMENT D -RESIDENT AND JUVENILE ANAOROMOUS PROJECT Background During FY 82 Resident and Juvenile Anadromous {RJ} investigations were initiated to describe distribution and relative abundance of resident and juvenile anadromous fish within the project area. The RJ studies conducted from November. 1980 to October, 1981 provided information on 11 known resident fish, five juvenile anadromous and one adult anadromous fish Sf)ecies, as listed below • Resident fish Rainbow trout Arctic grayling Burbot Rourtd whitefish Humpback whitefish (Coregonus sp.) Longnose sucker D~lly Varden/char Threespine stickleback Slimy sculpin Northern pike Arctic 1 amprey D-1 Juvenile Anadromous Fish: Chinook salmon Coho salmon Chum salmon Sockeye salmon Pink salmon Adult Anadromous Fish Bering cisco DRAFT Data co 11 ected by RJ crews during FY 82 have furnished va 1 uab 1 e baseline. data however, it is essential to continue and expand these studies over several years in order to accurately describe the seasonal distributions of resident and juvenile anadromous fish in the Susitna River and to determine the various biological and hydrological factors related to their presence. Sa~ling procedures based on FY 82 results will be implemented in FY 83 to permit a more comprehensive evaluation of seasonal fish distribution, biological, and habitat characteristics of the sites to be sampled. -, 191 the Phase II RJ stqdies. further information will be collected on male/female ratios Df ad-.alt residents. Additional seasonal distrib-.ation, relative abundance, and biological data will be gath"'r~ on juvenile residents and juvenile pink, chum, and sockeye salmon in order to evaluate habitat requirements for various stages of their life histories. The fact that Phase I data did not provide a basis for determining the rearing characteristics and habitat for sockeye fry and juveniles is one example of the need for further study in this subject area. Expanded studies proposed for Phase II wfll also compile more detailed information on location of -------·-·-·--------... ·-·---- mainstem and slough spawning sites, spawning habits and timing, incubation of embryos, and seasonal movements for all species. A primary interest will be to characterize the timing of spawning for burbot and grayling in the Susitna River. Another facet of the 1982 studies will be to collect and evaluate baseline benthic and fish stomachs data to provide the basis for developing a more comprehensive food habitat program in 1983 (FY 84). [ [ I '-~ [ c [ [ [ [ [ [ [ [ [ [ [ [ [ [ _j 1 j • 1 • ,, 1 J l j ' ~ DRAFT Positive results obtained from a limited amount of sampling with electroshockers in 1981 have indicated that expanded use of this technique for tagging and radiotelemetry will be of considerable value in Phase II studies. Use of a smolt trap is being incorporated into the FY 83 studies in order to determine timing of smoltification and outmigration of juvenile salmon from the Talkeetna to Devil Canyon reach. Additionally. a study has also been proposed for FY 83 to initiate an evaluation of transmission nd access corridor areas to determine potential impacts on fishery resources in these areas. This ....... .....__. study proposa 1 is based on the ADF&6 • s recommendations made in our November 1979 plan of study. The components of this RJ study proposal are: 1. Resident and Juvenile Anadromous Distribution Study 2. Anadromous Outmigration (Smolt} Study 3. Electrofishing Survey 4. Upper River -Impoundment Study 5. Upper River -Access and Transmission Corridor Study D-3 - - .. DRAFT Resident and Juvenile AnadTOmOus Distribution Study Background During FY 82 joint crews of Resident and Juvenile Anadromous (RJ) and Aquatic Habitat and Instream Flow (AH) personnel collected fishery and habitat data at 82 habitat location sites within the study area. These ---.. data provided a basis for describing the baseline seasonal characteristics ----------------------~------------of the distribution of fish within the study area and the general water chemistry and hydrological characteristics associated with fish tra.pping locations. This data collection will be continued and expanded in Phase II to further refine the Phase I data base and thus provide better understanding of general fish distribution,. timing of spawning,. rearing,. . incubation, and passage events and the general habitat characteristic;s of the Susitna River system. Resident and juvenile anadromous fish distribution studies will be conducted. unlike Phase I studies, solely by RJ personnel to permit AH personnel to concentrate on specialized habitat studies. Objectives A. Define seasonal distribution and relative abundance of resident and juvenile anadromous fish in the Susitna River between Cook Inlet and Devil Canyon. [ L r L [ [] '[ [ c [ [ [ [ [ [ [ [ [ [ [ • • DRAFT Task 1. Through direct field observations and measurements expand and refine the result of the 1981 RJ/AH Habitat Location Study regarding relative abundance., geographical and seasonal distribution, and movement of resident and juvenile anadromous fishes. Task 2. Record age class composition, length frequencies., sex ratios, and general life history infonnation on captured fish. B. Characterize the seasonal habitat requirements of selected anadromous and resident species within the study area • Task 1. Monitor water quality and staff gages at designated habitat locations. Task 2. Hap habitat characteristics and locations of sampling sites within each habitat location. Resident and juvenile anadromous fish distribution data will be collected from habitats located in the following 3 lower Susitna River areas. 1) Deshka to Talkeetna -Mainstem., sloughs~ and tributary mouths 2) Talkeetna to Devil Canyon -Mainst..l>J'R, sloughs, and tributary mouths 3} Indian River and Portage Creek -Tributaries D-5 • - DRAFT Work Plan Suaaer Field Season -May through October Two two-man crews will collect RJ habitat location data below Devil Canyon. One crew will be based out of a camp at Sunshine and will sample habitat locations between the Deshka River (RM 40.6) and Talkeetna (RM 97.0). This crew will also operate the lower east bank Sunshine Station fishwheel from ice out until the Adult Anadromous crew takes over on June 7. The second crew will have base camps at Talkeetna and Gold Creek and will be responsible for sampling habitat locations from Talkeetna (RM 97 .0} to Devn Canyon (RM@ 8oth of these crews will assist with the smolt trap at Talkeetna and sampling habitat locations on Indian River and Portage Creek. Tasks 1 and 2 will be accomplished by continuing the basic RJ/AH habitat location study program initiated in 1981. The following refinements will be made to the program: a. discontinue sampling locations downstream of the Oeshka River (RM 40.6) b. determine whether habitat locations in the Delta Islands to Devil Canyon reach should be added, dropped or modified and to select FY 83 locations. ~-·~ ~ample each habitat location for one 24 hour period each two ~ ' weeks. ~_.-~,-~----, -·------- [ 1: [ [ [ r , L __ j [ [ [ [ [ [ [ [ L L L L [ DRAFT Task 3 will be accomplished similar to the above. The Resident and Juvenile Anadromous crews will be trained by Aquatic Habitat personnel to measure aquatic habitat parameters and subsequently talce over the responsibility of collecting this data at habitat locations. Winter Field Season -November through April During this rews will conduct winter satnpling at habitat locations below Devil canyon. Two crews will be based at the Talkeetna ADF&G house at Talkeetna and will sample habitat locations between the Deshka River (Rf.f 40.6) and Mainstem 2 (RM 114.4). The third crew wi 11 work out of a cabin at Go 1 d Creek and wi 11 samp 1 e habitat locations between Curry (RM 120. 7) and Devil Canyon (RM 148.8). Habitat locations which can not be reached safeiy by snowmachine wiii be sampied on a reduced schedule via helicopter. D-7 DRAFl AnadrQIIIOUS Outmigration (Smolt) Study Background Previous stud-ies of the Susitna River have utilized minnow traps, beach seines. and electrofishing for the collection of juvenile anadromous species to characterize their early life histories. Minnow traps have not proven to be successful in certain lotic environments for the capture of pink, sockeye, and chum salmon juveniles. High rher velocities and uneven substrates conditions preclude the use of beach seines. Electrofishing is restricted ·~~s b~~nd~iv~ty~~~ _reduced visibillty.J A mainstem river station~ry fish trap and the installation of a weir at Iron Creek were originally proposed in the ADF&G Preliminary Final Plan of Study in November, 1979. A memorandum on June 2, 1980 to the Alaska Power Authority reiterated the Department's recommendation to establish a stationary fish trap and develop suitable sampling techniques for the collection of juvenile anadtomous species. However, neither the fish trap or weir were funded by the AP~i_n ':_~a,!~I_-~t_!!4j~s_. _______ ~_'" ___ _ ,_ ·-\"---="--· ··-"" .... ......_, The need for incorporation of additional sampling techniques to adequately determine the complete life histories of juvenile anadromous fish in the Susitna River has been a fundamental advice of the Alaska Department of Fish and Game. It is necessary to deploy a downstream migrant fish trap for the study of smoltification and oubiligration of juvenile salmon. Mainstem and slough surveys of salmon spawning sites should be conducted to D-8 ':) --~~--~~----~---------- [ [ [ [ [ [ r L; [ [ [ [ [ [ [ ~ r-' I -·~ [ [ [ L • • • DRAFT assess the spawning success. over winter survival, and emergence timing for these spec,ies. These techniques in conjuction with the standard sampling gear will provide the data base necessary for the characterization of the early life histories of juvenile anadromous species in the Susitna River. Objectives A. Determine timing of smoltification and/or outmigration of juvenile salmon from the Talkeetna to Devil Canyon reach of the Susitna River. Task 1. Determine the age class composition of coho and chinook salmon smolts/outmigrants. Task 2. Work Plan To provide qualitative estimates of spawning success, ov~r winter survival of eggs and alevin, and timing of emergence for slough and mainstem spawning salmon species between Talkeetna and Devil Canyon. The smolt trap will be located at Talkeetna Station and will be run continuously by a two man crew from July 1 to September 31, 1982 and the period imnediately following ice out to June 30, 1983. Size frequency and 0-9 • • DRAFT age analysis by species will be determined from subsamples of collected fish. Slough pre-emergent studies will be limited to the Talkeetna to Dcwi1 Canyon reach. Periodic pre-emergent sampling with standard pre-emergent gear will be conducted at known mai·nstem and slough spawning sites and beach seines and electrofishing will be incorporated as time and conditions allow. Surveys of known sockeye salmon spawning sites will be conducted utilizing pre-emergent gear, beach seines. and electrofishing to determine the distribution and rel'at1ve abundance of juvenile sockeve salmon. . - 0-lQ r- [ [ [ [ [ [ [ [ [ r- L~ [ [ [ [ • DRAFT Electrofishing Survey Backgr'OURd Ele~trofishing was conducted in early October, 1981 for 10 days by Resident and Juvenile (RJ) and Aquatic Habitat (AH) personnel in the mainstem Susitna River between river mile 70.0 to 105.5 utilizing a boat mounted electrofishing unit. Sampling of resident fish during this time, had three objectives: {a) locate mainstem river spawning areas of various species of resident fish. (b) conduct a tag and recapture program. and (c) to capture adult resident fish for the radio telemetry program~ Electrofishing during October resulted in locating mainstem Susitna River spawning of Bering cisco. Sexually mature round whitefish in spawning condi.tion were also captured in the mainstem Susitna River. Two hundred and ten resident fish and 312 Bering cisco were collected and floy tagged during the 10 days of electrofishing. Fifteen fish were recaptured, during the sampling trip. Electrofishing was also utilized during this time to capture adult resident f1sh for the radio telemetry program. Electrofishing was utilized due to the high survival rate of fish .collected by this sampling method (greater than 951) than other metf:l·: js (gillnets and trotlines) • Electrofishing conducted in the mainstem Susitna River in October demonstrated the practicality of boat mounted e1ectroshocking units as a D-11 DRAFT sampling method in the Susitna River. Due to mobility, little interference to elec.trofishing was caused by the effects of the Susitna River (water velocity, debris, etc.) which liaited the effecthness of other sa~~pling gear (gillnets. etc.). Also, due to the mobility of electrofishing gear, interference with the public was not encountered nor is anticipated in future sampling. lhe practicality of expanded sampling utilizing electrofisbing is further deoonstrated by the relatively, high capture and survival rates of adult resident fiSh electrofished compared to other sampling methods. Due to these reasons so. .. ; 1 ing utilizing boat mounted electrofishing units should be expanded to further define distribution and relative abundance and to characterize the seasonal habitat requirements of anadromous and resident species within the study area. Objectives A. Define distribution and relative abundance of resident and juvenile anadromous fish in t~ Susitna River between Cook inlet and Devil Canyon. Task 1. Identify life functions (i.e. spawning, rea~ing, migration, feeding} associated with the mainstem Susitna Rher habitats. Task 2. Record descriptive information on captured fish and discuss seasonal migration patterns on selected adult resident species. D-12 [ [ [ [ [ [ [ [ [ [ [ [ - [ [ [ [ [ L -l ~ ~ ~ ~ "" iJ .. ~: j _, _{ "i ·-.< ... -I i ! 1 -" B. DRAFT Task 3. Carry out adult anadromous fish studies as outlined. Task 4. Work in conjuction with the adult anadromous fish project on the expanded resident fish radio telemetry studies. These are discussed in the AA Project plan of study. Characterize the seasonal habitat requirements of selected anadromous and resident species within the study area. Task 1. Collect aquatic habitat and instream flow data, i.e. point velocities and conductivity data as detenmined by AH personnel. Task 2. Provide locations of fish and ~~ data to the ~~ point specific crew. Work Plan Electrofishing, incorporating a boat mounted electrofishing unit, will be conducted from July 1, 1982 to freeze-up. Two RJ biologists will utilize the electrofishing equipment; and, if a third person is needed to sample on occasion, he will come from existing staff. Objective A, Tasks 1 and 2 will be accomplished by periodically sampling a number of pre-selected locations. Additional sampling (up to 331 of the D-13 DRAFT total effort) will be conducted in locations selected in the field in an attempt to locate concentrations of fish. Tagging 9 tag recovery. and collection of biological data will also be conducted. Tasks 3 and 4 will be to determine slough and mainstem Susitna River spawning and milling areas of adult anadromous fish as outlined in the Adult Anadromous (AA) study• however9 all costs to accomodate these tasks are contained in the RJ program. Objective B. Tasks 1 and 2 to characterize aquatic habitat conditions are discussed in the AH study. ~ Field stations for this program will be located at Sunshine, Talkeetna. and Gold Creek, during the open water season. • Equipment budgeted by AH will be provided to the RJ project to characterize the habitat requirements of selected anadromous and resident species. All other costs are li~ted herein. -'tg·· r· L~ [ [ [ [ [ [ [ [ [ [ [ [ [ [ [ [ L ., ~ ~ ' ..Ji __,; -, "' -., ~ . ' J ' .... DRAFT Upper River-Impoundment Background Phase I Upper River Impoundment Studies were conducted by joint RJ and AH crews to identify resident and juvenile anadromous fish species, their relative abundance and the general aquatic habitat characteristics in areas which would be tnnundated above Devil Canyon. Additional investigations were conducted to identify, on a preliminary basis, habitats above the impoundment which would be available to the existing fishery if the dams were built. Phase II investigations are designed to refine and augment the Phase I studies-A primary concern will be to focus the FY 83 activities on the stretch of river immediately above the proposed impoundment elevations. Other acthities will be initiated to provide better fish distribution in adjacent lakes and a better knowledge of fishe~ activi~ in the mainstem Susitna River. These investigations will be performed by a joint RJ/ crew on a year round basis. D-15 / I DRAFT Objectives A. Oetennine the seasonal distribution and relative abundance of selected resident fish populations within the study area above Devil Canyon. Task 1. Identify passage. spawning, incubation~ and rearing locations selected resident species and estimate their comparative importance. Task 2. Record descriptive information for fish captured and discuss seasonal migration patterns of selected adult resident species. B. Characterize the seasonal habitat requirements of selected anadromous and resident species within the study area (refer to age study plan). Work Plan Sunmer Two biologists. 1 RJ, 1 AH will operate out of a proposed tent camp located at the mouth of Watana Creek. An AH crew leader will also participate on a part time basis. The study area for Phase II field work will include the one mile reach of each of the eight major tributaries upstream from the Q•l6 [ [ [ [ [ [ L [ [ [ [ [ [ [ f' L [ [ l [ DRAFT proposed impcundrlent elevation, {the projected location of the new •mouth•), a nua~er of habitat locations in the Susitna and its sloughs, the Upper Deadman River syste11 and the eight existing habitat locations. Access from Anchorage to the study area will be via chartered fixed wing aircraft. Access to the actual habitat locations will be via Acres helicopter and ADF&G riverboat. The sampling plan will provide for: 1. Monthly visits to the eight tributary mouth habitat locations sampled in 1981 2. Monthly visits to 5 mainstem and slough habitat locations (to be selected in spring 1982) 3. Population estimates for selected resident fish species in the lake located at 32N 07E 29. 4. Evaluation of the Upper Deadman and Deadman lake system grayling and lake trout populations 5. One time only evaluation of grayling population and aquatic habitat located in the reach of each tributary beginning at the proposed impoundment elevations and extending one mile upstream. These surveys will be conducted in July and early August when grayling distribution is most extensive. • • DRAFT Winter Three biologists will conduct two five day sampling trips. one each in early March and in late March or early April using Acres helicopter. Work will concentrate on the Susitna and potential overwintering locations in each tributary. Watana camp will be utHized as a base of operation. l" [~ [ [ [ [ [ [ [ [ [ [ [ [ [ [ [ L D-IS-~------[ • DRAFT Upper River-Access and Translrission Corridor Study Background Phase I studies did not include fishery and habitat evaluation of the proposed access and transmission corridors as proposed by the AOF&G in 1979. Therefore. the proposal below has been designed to generate baseline data to provide a basis for evaluating the impacts of the proposed Access and Transmission Corridor. Objectives A. Determine the open wa~er seasonal distribution and relative abundance of selected resident and anadromous fish populations within the access/transmission corridors. Task 1. Identify passage, spawning~ incubation. and rearing locations and estimate their comparative importance. Task 2. Record descriptive information on captured fish and discuss seasonal migration patterns. B. Characterize the seasonal habitat requirements of selected anadromous and resident species within the study area (refer to age study plan). DRAFT The study area and information collected by location for the access/transmission corridor fisheries evaluation are outlined below: 1. Indian River, 3 east side tributaries, Miami take a. Adult salmon enumeration/timnng b. Adult salmon spawning distribution c. Juvenile salmon seasonal distribution by species d. Adult resident seasonal distribution and relative abundance e. Adult resident spawning distribution and timing f. Juvenile resident distribution g. Aquatic habitat evaluation for each of the above 2. High take lakes area 5 lakes a. Resident fish species composition b. Resident fish population age class composition c. Resident fish population relative abundance d. Aquatic habitat evaluation 3. Devil Creek {Upper) a. Resident fish species composition b. Resident fish species age class composition c. Resident fish species relative abundance d. Aquatic habitat evaluation 4. Tsusena Creek {Upper) a. Resident fish species composition D-2Q' [ j [ . ' ·[., ·[ r -, -~ [ [ [ [ [ [ [ [ [ [ L [ l [ ~ • " -' , "": .., • ...;' -" _. ,, .J DRAFT b. Resident fish species age class composition c. Resident fish species relative abundance d. Resident fish species spawning location e. Resident fish species intrasystem movements f .. Resident fish species timing of d and e above g. Resident fish species rearing distribution h . Aquatic habitat evaluation Work Plan Field activities will bel 1 imited to ice free months between July 1, 1982 and October 15, 1982, resuming May 1, 1983 through June 30, 1983 • A crew of three biologists two RJ, one AH will operate with ADF&G charter and Acres helicopter support out of Gold Creek and Watana beginning July 1, 1982, RJ will provide 1 FBII crew leader, AH will provide 1 FBI on a full-time basis who will be supported by his AH crew leader on a part-time basis. 1. Indian River a. b • Adult Anadromous: 1} A total of four aerial surveys once each two weeks beginning July 25, 1982 2} Ground truth surveys on selected reaches and in east side tributaries Juvenile Anadromous: 1) Select five sites utilizing the four 1981 --------------- • • DRAFT sites 2) Minnow trap 10 traps each site once per month through October 3) Minnow trap/electrofish east side tributaries 4) Recotd species composition subsample total length. catch rates c. Resident Fish Species: 1) Concentrate on rainbow and grayling 2} Hook and line at mwth minimum of 5 man-hours per montht t.tg and release, record bio data 3} Operate electro fishing boat at mouth each month 4) Hook and line at selected sites upstream each month 2. Alpine Lakes (5 lakes) a. Test net prior to August 15; if grayling or lake trout are present test net again prior to freeze up and coiiect bioiogicai data b. Aquatic habitat evaluation described in the Aquatic Habitat project 3. Upper Devil Creek a. Hook and line/electro fish twice during ice free months once July 1 -Aug~st 15, once August 15 to freeze up. Collect biological data and tag fish. b. Aquatic habitat evaluation 4. Tsusena Creek a. Grayling -1) Once monthly hook and line and electro fish 2) Bio data and tag 3) At least once during ice free months preferably late July early August sample upper reaches • • ATIACHMENT D-1 RESIDENT AND JUVENILE ANADROMOUS PROJECT Line 100 -Per~onal Services PCN Class Grade - 4184 FB II 16 4204 FB II 16 4201 FB II 16 4200 FB II 16 4205 FB II 16 4206 FB II 16 4204 FB I 14 4198 FB I 14 4197 FB I 14 4203 FB I 14 4199 FB I 14 *NEW FB I 14 *NEW Ffi I 14 Line 100 -Personal Services Subel~ment ( sutrmer) (w·inter) Habitat location Study Electrofishing Survey Outmigrant Study Impoundment Study Access/Transmission Study Data ~recessing Coordinator Line 200 -Travel Habitat location Study (summer) {winter) Electrofishing Survey Outmigrant Study Impoundment Study Access/Transmission Study Line 300 -Contractual Services Habitat location Study (summer) {winter) Electrofishing Survey Outmigrant Study Impoundment Study Access/Transmission Study D-23 MM 12 12 12 12 12 12 i2 11 11 11 9 9 6 Subtotal Cost 53.7 43.6 43.6 43.6 42 .. 0 42.0 37.8 34.7 34.7 34.6 28.3 27.4 18.3 484.'! 167.3 78.2 81.4 60.2 53.6 43.6 r~ [ [ [ [ [ [ [ r L c [ Subtotal 484.3 [ [ [ Subtotal Subtotal 2.7 1.0 .9 1.0 0.5 0.5 D:O 10.4 6.8 3.3 [ [ 2.5tt [',· 11.5 . 30.7 . I, 65.2 [ ~~----____, ........... ......_... [ • • • ATTACHMENT D-1 CONTINUED line 400 -Connodities Habitat Location Study (summer) (winter) Electrofishing Survey Outmigrant Study Impoundment Study Access/Transmission Study D-24 29.6 15.7 13.1 8.1 4.9 5.2 Subtota 1 7lL6 Grand Total (Line 100 -400) 632.7 ------~------------------- • • ATIACHMENT D-2 RESIDENT AND JUVENILE ANADROMOUS PROJECT Line 500 -Equipment to be purchased through Acres American. Inc. Item Quantity Description 1 2 Truck 2 2 Snowmachine 3 1 Riverboat (20') console and controls 4 2 Riverboat (18') w/ 48" bottom 5 1 Outboard engine, 75 h.p. 6 1 Jet unit 7 2 Outboard engine, 35 h.p. 8 2 Outboard 1 i ft 9 1 Outboard engine, 15 h.p. 10 1 Spillsbu~ base radio w/accessories 11 6 Wall tents 12 1 Tent, three person 13 1 Firearm D-25 Unit Cost Total Cost 10.8 2.0 3.0 1.6 2.3 0.9 1.1 0.4 1.0 2.3 0.4 0.4 21.6 4.0 3.0 3.2 2.3 0.9 2.2 0.8 1.0 2.3 1.5 0.4 0.4 43.6 [ [ [ [ [ [ [ [ L .[ [ L ~~---[ l. CJ ~ I. " • Anadr,omous Outmigrant Study I FB II PCN 4200 I FB I/II PCN 4202 ~. ..J li.L ' .u ,_, El ectrofishi ng Survey FB II PCN 4201 I FB I/ II PCN 4197 L ;, ,J b ' J k:l ' .• 1 • PROJECT LEADER FB Ill '" •• .J Data Processing Coordinator FB II PCN 4204 ~ .. ,; ~ ,i -" • Distribution Study Impoundment Study Access and Transmission FB II FB II FB II PCN 4206 PCN 4205 PCN 4184 I I FB l/11 I , PCN 4199 Sunshine Gold Creek I I FB 1/II FB 1/11 PCN 4198 PCN 4203 I I FB I/1 I FB Ill I PCN PCN :0 :Po , ::t VI -:Po 0 n , ::z: z 3: _. "" z ):a _. :z CJ CJ • c... w c:: < ~ -,... , :Po :z ~ ~ 5 c:: VI .., -VI ::z: "" ::0 ..... , VI "0 ::0 0 c... "" n _. DRAFT ATTACHMENT E -AQUATIC HABITAT AMO INSTREAM FLOW PROJECT Background Du•·ing FY 82 Aquatic Habitat and In stream Flow (AH), investigations were initiated to describe physical and chemical characteristics of various types of fish habitat within tfie project area. The AH studies also provided information necessary for testing the accuracy of physical (disc~arge and temperature) models which are being used to predict post-project impacts of the proposed dcm on aquatic habitat characteristics. The FY ~2 data indicated that the models need refinement and additional field data must be collected to calibrate them. Used in conjuction with the rest of the project studies, the aquatic habitat information clearly demonstrates that clear water sloughs provide the most important salmon spawning habitat of the habitats evaluated this year. in the Devil Canyon to Talkeetna segment of the river. ,[ f : ~~", [ [ [ [ [ [ L [ [ [ [ [ [ [ Although the AH studies concentrated on slough and side channel habitats, Adult anadromous and resident and juvenile anadromous fish distribution studies conducted in 1981 indicated the use of [ ~~L ,--_ =-:_-_-~-~-- • • DRAFT mainstem spawning habitat for chum and coho salmon and Bering cisco. FY 83 Phase II investigations have therefore been designed to characterize these mainstem spawning habitatsjand the influence of incremental changes in discharge on the mainstem habitat. ,u ~ Another facet of the 1gs1 studies was describing point specific fishery habitats at fish trapping sites. It will be necessary to expand these studies and conduct comprehensive point specific evaluations for each life phase of selected fish species in various habitats within the study area during FY 83. This information will then be used to generate habitat utilization criteria in terms of depth. substrate, and velocity~ dissolved oxygen, ph, temperature, and conductivity, for the various life phases and fish species studied .. These point specific data, when combined with selected habitat data which identify the quantity of habitat having certain velocity, depth substrate, and water quality characteristics at various discharges. can then be used to define impacts and mitigation strategies for the project based on the concept of flow regulation. Preliminary aquatic habitat investigations conducted in the area above Devil Canyon during 1981 were directed at deternrining the physical and chemical characteristics of fish habitat which would be inundated by the proposed impoundment. Eight major tributaries were selected for study in this area and investigations centered on the lower mnle of each. less detailed E-2 ' DRAFT studies we~e conducted on the uppe~ areas of these streams, the mainstem Susitna and various sloughs and side channels. These studies were initiated to determine the extent of loss of habitat to resident species in this area most no~ably the Arctic grayling. Investigations will be expanded in FY 83 to include more detailed studies of the mainstem Susitna. and sloughs and side channels which might be utilized as spawning or rearing habitat by grayling. Also, the upper areas of the selected tributaries directly above the pr~~osed impoundment elevation will be examined to determine if this are~ provides similar habitat to that now present at the mouth of each stream. Because 1981 studies did not include an evaluation of transmission c.nd access corridor areas., studies have been proposed for FY 83 to initiate an evaluation of these areas. Complete habitat evaluations will be conducted at all areas where stream crossings may occur or where construction of any type may have an impact on water quality or stream channel characteristics. These studies will provide the basis minimizing or avoiding impacts along these routes. r L .. [ [ r--1 [ [ [ [ [ c [ [ [ [ L [ [ The large amount of data generated and the time and manpower resources required to reconcile., reduce, and verify the accuracy of these data in preparation for transfer to the data processing support unit underestimated in FY 82. To correct this problem, the Phase II FY 83 AH program has been organized to insure personnel will be assigned to insure data are properly treated prior to transferring it L .~~~l E-3 • • • DRAFT to the data processing support unit. Phase II AH studies will be interrelated and coordin.ated with other fisheries and related project studies to insure maximum and efficient usage. of project resources. Phase II FY 83 AH studies wi 11 be subdivided into two se9Jnents: 1) point specific fishery habitat evaluations (Tasks 1.1 and 1.2) of the principal resident fish. and juvenile and adult anadromous salmon habitats; and 2) selected stream flow dependent habitat evaluations of slough and mains tern habitats in the study area (Task 1.3) • E-4 - - Objective 1. Characterize the seasonal spawning. incubation, rearing, and passage habitat requirements of selected anadromous and resident species within the study area. DRAFT Task 1.1 Through direct field observations and measurements identify the physical and chemical conditions which appear to be influencing the suitability of various habitat types for the species and life history stages of interest. Task 1.2 Through direct field observations and measurements characterize the physical and chemical parameters of the various habitat types found in the study area. Task 1.3 Through direct field observations and measurements, characterize the influence of a range of flow regimes on the physical and chemical parameters associated with selected habitats. The AH program study area is divided into 3 geographical areas: 1) Cook Inlet to Devil Canyon 2) Devil Canyon to Tyone River 3) Corridor Study Area [ [ [ r_~ [ [ [ [ ,, I L [ [ [ r~ L [ L j [' I .. 1 [ [ ~------...:..~ ............... .....:.........----.................... --~ [ • j ' ~ .,. • _. "" -" ~ j DRAFT Point Specific Evaluations Background The large number of FY 82 fish sampling sites primarily restricted the collection of point specific depth, velocity. and substrate data to juvenile anadromous fish trap sites. Because these data are required to identify the physical conditions of the river which appear to influence the suitability of various habitat types for the species and life history sta.ges of interest,. a major objective has been placed on expanding point specific data in FY 83 . The primary emphasis wiii be directed toward identifying point specific characteristics of mainstem, slough, and side channel spawning habitats for the adult salmon and selected resident species such as burbot and grayling. Additional efforts will be focused on collecting point specific data for rearing, incubation and passage habitats. Dissolved Oxygen, ph, temperature and conductivity data will also be collected in the vicinity of point specific evaluation sites to characterize . the overall general water quality characteristics associated with fish activities and useage of habitats. Tasks 1. Through direct field o.bservations and measurements E-6 .... .. Work Plan -- DRAFT identify the physical and chemical conditions which appear to be influencing the suitability of' various habitat types for the species and life history stages of interest. Open Water Season ~ AH crews will collect point specific velocity, depth, and '--substrate data below Devil Canyon. One crew will be assigned to an electrofishing boat and coordinate with AA and RJ electrofishing crews in addition to the three other AH point specific crews. The AH electrofishing crew will locate and sample fish habitats between the estuary and Devil Canyon. The other three point specific crews will subdivide the estuary to Devil Canyon Study area into 3 river segments; 1) Estuary to Kashwitna 2) Kashwitna to Talkeetna; and 3) Talkeetna to Devil Canyon The three crews will employ a variety of fish sampling techniques to identify fish locations prior to characterizing depth, velocity, and substrate characteristics of the water column occupied by the fish. Conductivity, ph, temperature and dissolved oxygen data will also be [ [ [ [ l' L [ [ [ [ [ [ [ [ L J ~1 _J ··- DRAFT collected at these locations. Supplemental dissolved oxygen., temperature, stage., and conductivity data will be collected at habitat locations as part of the RJ Fhh Distribution Study by RJ staff. Aquatic Habitat personnel will train the RJ crews to collect the AH data. Conductivity data will also be collected by AH, RJ., and AA electrofishing boat personnel. Velocity and depth data will also be collected by AH, RJ, and AA electrofishing crews at fish spawning locations. Ice Covered Season During this sampling period two AH crews will continue to collect point specific data between the Estuary and Devil Canyon to characterize ice covered point specific fish habitats. E-8 • I I -~ !1 ] 4 l I • DRAFT Selecte~ Habitat Evaluation In FY 82 Selected Habitat Evaluations of discharge influenced water quality and hydrologieal characteristics were conducted at five sloughs upstream of Talkeetna, and side channels and mainstem river sites located between the estuary and Devil Canyon. These data enabled project r.ersonnel to evaluate the accuracy of hydrological and water temperature models which will be used to predict discharge influenced impacts on fishery habitats. The FY 82 data clearly demonstrated the impertance of these studies and need to expand this data base in FY 83 if the goals of defining discharge influenced impacts to fishery habitats by the proposed project as well as designing discharge related mitigation options are to be achieved. Tasks 1. Through direct field observations and measurements characterize the physical and chemical parameters of the various habitat types found in the study area. 2. Through direct field observations and measurements characterize the influence of a range of flow regimes of the physical a.nd chemical parameters associated with selected habitats. [ [ r lr jl ' ' ! l _j [ c [ [ .C [ L [ r~ [ [ DRAFT Work Plan -- Open Water Season lM:I IIJObile AH crews will collect selected habitat data between the CD .... ..t......,._ <!:> Cook Inlet and.ADevil Canyon at slough and 111inste111 locations. The pri~~ emphasis of their activities will be directed towards expanding the data base at ttte five slough areas evaluated in Phase t and developing a similar data base at mainsteat spawning sites between Talkeetna and Devil Canyon. Surface water thennognphs and staff gages (surveyed to datums} will be installed at selected locations in the study area between Cook Inlet and Devil Canyon survey study sites, and collect instantaneous reading of dissolved oxygen, ph temperature, and conductivity collected. Additional thermographs will be installed to characterize relationships between surface and groundwater sources at spawning locations. Other efforts will be directed towards e.xamining discharge habitats below Talkeetna. tee Covered Season One AH crew will continue collecting selected habitat data throughout the winter field season. E-10 • • DRAFT Upper River Study -Impoundment Background Fiscal Year 82 studies were designed to determine the impacts of ~erting this lotic system into a·lentic environment by the proposed reservoirs. Fiscal Year 82 Investigations were limited to the lower mile of eight tributaries studied and less comprehensive evaluations of the upper reaches of these tributaries in the vicinity of the proposed impoundment elevations. Fiscal Year 83 studies are designed to refine the FY 82 data base and better evaluate the potential of the lotic habitat located immediately above the proposed reser~oir surface elevations to substitute for the existing lotic habitats. Tasks: 1. Through direct field observations and measurements identify the physical and chemical conditions which appear to be influencing the suitability of various habitat types for the species and life history stages of interest. 2. Through direct field observations and measurements characterize the physical and chemical parameters of the various habitat types found in the study area. [ 0 n L c c [ [ [ r~ L J[ [ [ E-ll -·· ······~··~---------[ r .. DRAFT Wort Plan Open Water Season One full time and one part time AH biologist will work with RJ biologists in conducting fishery and aquatic habitat evaluations in the proposed impoundment area. A combined crew of AH and RJ biologists will collect conductivity, ph, dissolved oxygen, and temperature data at all sampling locations. Depth, velocity and substrate data will be collected at selected sites to characterize spawning and rearing habitat of species of interest. The most intensive studies will be undertaken during the open water season • During this time operations will be conducted out of a proposed tent camp located at the mouth of Watana Creek. A more detailed description of objectives and tasks related to this study is presented in the RJ Impoundment Plan Of Study (POS). Budgetary items not included in the RJ POS are incorporated in the AH program where appropriate. Ice Covered Season Three biologists will conduct two five-day sampling trips, one each in March and April. Work will concentrate on locating major overw'\ntering· habitat in the main Susitna and selected tributaries. Transportation will be by snowmobile with Acres helicopter support. Operations during this time will be conducted from Watana Camp. " ... DRAFT Upper River -Access and TranSitission Corridor Study Background The ADFIG proposed studies for evaluating Access and Transmission . Corridor in October 1979. Because these studies were not funded in FY 82, they are again being proposed for FY 83. The ADFIG considers an evaluation of these areas to be an integral part of the overall Su Hydro project feasibility assessment. Tasks: 1. Through direct field observations and measurements identify the physical and chemical conditions whith appear to be influencing the suitability of various habitat types for the species and life history stages of interest. 2. Through direct field observations and measurements characterize the physical and chemical parameters of the various habitat types found in the study area. [ [ [ r L r Lj [ [ [ I' L [ [ [ [ [ L [ [ [ _E-13 ·-·~'-'---' ___ __... ....... ...._ ............ L • 1 .. '!! • -, -, •• DRAFT Work Plan Open Water Season One full time and one part time AH biologist will work with RJ biologists in conducting the Access and Corridor studies. These studies will be conducted during the open water field season only, beginning approximately July 1, 1982 to October 15, 1982; resuming approximately May 15, 1983 to July 30, 1983. AH and RJ data will be collected concurrently while in the field. AH data will include conductivity,. ph7 dissolved oxygen and temperature. Depth, velocity, and substrate data will be collected at selected sites to characterize spawning and rearing habitat of species of interest. RJ data collection plans are discussed in the RJ study plan section. Operations will be conducted out of Gold Creek and Watana Camps utilizing Acres helicopter support. A more detailed description of objectives and tasks related to this study is presented in the RJ Access and Corridor Plan Of Study {POS). Budgetary items not included in the RJ POS are attached • E-14 [ r ATTACHMENT E-1 [ AQUATIC HABITAT AND lftSTREAM FLOW r~ line 100 -Personal Services L_; PCN Class Grade "" Cost [ -j -4208 FB I-II 14/16 12 40.5 4196 FBU 16 12 42.6 c 4195 FB II 16 12 42.3 4211 FB II 16 12 41.6 q 4126 FB I-II 14/16 12 37.2 4209 FB I-II 14/16 9 28.3 r: 4210 FB I 14 10 31.0 ' -.:__ -4207 FB II 16 12 42.3 *NEW FB I 14 12 35.8 0 " *NEW FB I 14 9 26.9 ' q *NEW FB I 14 9 26.9 'V *NEW FB I 14 9 26.9 \. .. *NEW FB I 14 12 35.8 [ *NEW FB I 14 10 29.8 *NEW FB I 14 10 29.8 . __.~flEW FB I 14 9 26.9 [ (b *NEW FB I 14 10 30.8 *liN ~ll T 14 10 29.8 .~ .... ' ., .. *NEW Hyd-II 16 10 34.0 *NEV Hyd-II 16 12 40.8 [ *NEW FT -II-III 11 12 28.0 108.0 tine 100 -Personal Services by Subelement c Subelement Cost Data I tab I Logistics 68.5 [ Selected Habitat 233.9 Point Specific 302.7 [ Upper River I Impoundment I A&T Corridor 42.3 Impoundment Study 30.8 Access and Transmission (A&T) Corridor 29.8 708.0 [ line 200 -Travel r AH Administration 5.0 II l . Data I Lab I Logistics 1.5 _j Selected H.abitat 15.5 Point Specific 12.3 [ Impoundment 3.7 A&T Corridor 0.-5 ~ [ ...... L • • ATTACHMENT E-1 CONTINUED line 300 -Contractual Services AH Administration Data I Lab I logistics Selected Habitat Point Specific Impoundment A & T Corridor Line 400 -Commodities AH Administration Data I Lab I logistics Selected Habitat Point Specific Impoundment A & T Corridor - E-16 Grand Total 8.0 11.3 36.7 31.6 5.3 3.9 96.8 2.8 8.5 38.6 58.7 7.7 1.9 118.2 (line 100 -400) 961.5 • • ATTAaHMENT E-2 AQUATIC HABITAT AND INSTREAM FLOW PROJECT Line 500 • Equipment to be purchased through Acres American, Inc. Item Quantity Descripti,·on Unit Cost Total Cost 1 8 Hydrolab & accessories 4.0 32.0 1 2 7 Barometer 1.1 _j 3 13 Thermograph -Ryan 5.0 6.5 1 4 1 Marsh-McBirney meter 2.5 2.5 -" ~ • 5 8 Price AA meter 7.4 6 7 Wading rod, topsetting 0.5 3.5 ' 7 7 Wading rod, conventional 0.3 2.1 "' . 8 4 Propane stoves 0.7 " 9 2 Tagl ine 0.5 _. 10 150 Staff Gage 2.7 11 2 level 2.0 4.0 12 1 Rod 0.2 0.2 " 13 1 Tripod 0.2 0.2 14 2 Camera~ compact 35 mm RF 0.3 ~ 15 . 180 Trap 1.3 "' ' 16 15 Net 1..8 j 11 1 Electroshocker, backpack 1.5 1.6 ~· 18 3 T~gging gear set 0.2 0.6- "' E-17 -' [ [ • ATTACHMENT E-2 CONTINUED [ Item -Quantity Description Unit Cost Total Cost [ 19 2 Egg pump 1.5 3-0 [ 20 6 Riverboat (console and controls) 3.0 18.0 I' L-' 21 6 Outboard engine. 75 h.p. 2.5 15.0 22 6 Jet unit 1.0 6.0 [ 23 6 Outboard engine, 15 h.p. 1.5 9.0 24 5 Boat trailer 1..7 8.5 [ 25 4 Snow machine 2.0 8.0 ~ 26 3 Snow machine trailer 0.9 2.7 L • 27 3 Sled 0.6 [ 28 5 -• I ,.., J rucK \new or useo1 i0.5 52.5 29 3 Ice auger 0.6 1.8 [ 30 6 Shotgun 0.4 2.4 31 4 Chainsaw 0.5 2.0 [ 32 6 Walkie Talkie 0.9 [ 33 11 Wall tent 2.6 34 9 Pack tent 3.2 [ 35 5 Camera, 35 nt11 SlR with lenses 0.6 3.0 36 1 Substrate camera 0.5 0.5 r-· L 37 2 Backpack 0.3 38 2 Ice meter 0.4 0.8 1L 39 2 Ice rod 0.5 1.0 [ • 40 2 Lenses, for 1981 35 mm Came~(SlR) 0.5 1.0 [ E-18 L ATIACHMEfH E-2 CONTINUED Item Quantity Des~ription Unit C.os,t Total Cost 41 10 Thermograph -Datapod 1.5 15.0 42 1 Boom, aluminum for 2.5 2.5 43 1 Refrigerator 0.8 o.s 1 44 1 Freezer 0.6 0.6 _, 45 1 Slide projector and accessories 0.6 0.6 1 46 2 $pillsbury radio 2.3 4.6 -" 47 9 Cot 0.1 0.9 1 __; 48 10 Sleeping Bag 2.5 """! • 239.3 _3 -_. '1 _. ' - ~ -'" I ·• E-19 ITt I ~ ~ ta.c_ ( ....14 W: ... " .Li.JJ IJ,, I _:;) • " ' Jl • AQUATIC HABITAT AND INSTAEAM FLOW $1\.I[)V Table ol Clrganlzallon PtolectLeader FIB .• PCNI4192. ---·t= - Oala_ Cootrol, La,boratory and LOQisllcs Coordinator FB &IU .,~ PCN 4208 t.A_~ --~~\ ~------. , I . ~IM'Idment and. Access Slucl'as FB I • PCN~4207 I . r-~---. 7 1 Acceo,an!l T,ansmlsalon ~~ SII.A' I . I . FBI/I PCN-' ....... FBUI PeN_ Sei~Jcled HabU111 s .• udV F&I·PCN-,4186 ~. ~~~·~l~if FB Ul FB lltl FB. Ill FB Ill PCN_ f'eN.-F'ON_ PeN_ '·~~~ ,;\ Gold Cleek I F.B Ill PCN 4126 I FB'I/1 PCN_ ,c ·Pofte · Speclftc · Sutv FB I· PCN 4195 Eleclrol!lli*IO 'F8·1 T•llcrlnl EB, Ill PCNr20t FB Ill PCNI42H .FB Ill PCN· •210 &Mhine I F.B Ul PCN_ I FB lilt PON_ PCN_ • > > g :1· ~ > ('"') -5 ('"') , ;; ::;:: ..... CCI -ITt -t I ~--w > z c -z Vl ~ i ., .- i "Q :a E ITt Q DRAFT ATTAOIIOO F -ADUlT ANAORCJIOUS PROJECT Backgr_ound Under Phase I studies, intensive monitoring of one adult salmon escapement year in the Susitna River was cOmpleted. Recognizing that an escapement cycle encompasses a multiple year period (Example-: pink salmon are two year old fish; chum. sockeye 9 cohp and chinook salmon are predominately four, five, four and four year old fish. respec- tively) it is essential that at minimum, a complete life cycle be evaluated. Baseline, pre-project data on anadromous fish distribution, abundance, timing and migration characteristics is vital in assessing the resource and related impact of development and operation of a hydroelectric facility on the Susitna River. In addition to salmon, there are two other anadromous species that inhabit the Susitna River. Th~' are: Bering eisco and eulachon. The former species was documented for the first time in the Susitna River during Phase I investigations. The 1981 ci·sco escapement was com- prised predominately of four year old fish. It is therefore warranted to monitor escapement distribution, abundance, timiog and characteristics for at least three additional years on this specie~ cover one cycle. The eulachon population in the Susitna River has not been studied • Baseline information is ne.eded on the timing, relative [ [ [ [ [ [ [ [ [ [ [ [ [ L [ ~ , ~ .1 -- • OAAIT • abundance, and spawning areas of this species to define probable impacts of hydroelectric devel~t .. Prior to Phase I studies, population estimates were obtained for pink salmon in 1974, 1975 and 1977. Chum and sockeye salmon escapement estimates were obtained in 1974 and 1975.. Coho salmon escapement estimates were not obtpined prior to 1981 Phase I studies. Several years of pre Phase I chinook salmon escapement data is available on tributary spawning streams but no mainstem Susitna River estimates have been obtained either prior to or under Phase I studies. Phase I investigations successfully characterized 1981 escapement for pink. ~ockeye, chum and coho salmon. Additionally. this study defined for the first time mainstem spawning and notable milling activi~ by chinook, sockeye, chum and coho salmon at the entrance to Devil Canyon. Several new salmon spawning sloughs and streams were also located .. Phase I escapement levels were characterized at three locations: Susitna Station, Yentna Station and Sunshine Station. The base line data collected during the 1981 escapement season can not be used alone to characterize Susitna River production. To accurately ascertain Susitna River production trends, escapement monitoring should continue through at least one complete life cycle. F-2 • • • DRAFT Slough areas. side channels and tributary confluence areas should be surveyed concurrently with escapement monitoring to provide a reliable pre-project data base on use of these habitats. Radio telemetry investigations on adult a:nadromous and selected resident fish should continue for at least four consecutive years to monitor and milling patterns under variable environmental conditions and migratinn strength. Objective • The overall objective of the FY 83, Phase II Anadromous Adult Project will be to: 1. Determine annual distribution and relative abundance of adult anadromous fish populations within the study area. Tasks under this objective are as follows: Task 1.1 Task 1.2 Task 1.3 Enumerate and characterize the adult anadromous fish miqration. Detenmine the timing and nature of migration, milling and spawning activities. Identify spawning locations t-!ithin the study F-3 [ [ [~ [ [ [ [ [ L [ [ [ [ [ I' L [ [ [ L • • DRAFT area 1 ikely to be affected by post project conditions (i.e. mainstem, side channels, sloughs and tributa~ confluences). and estimate their comparative importance. All adult anadromous fisheries studies will be interrelated. However, the program proposed is divided into two major geographical areas: 1) Cook Inlet Estuary to Talkeetna 2) Talkeetna to Devil Canyon A summary of the proposed program by geographical area follows: I. Cook Inlet Est~ary to Talkeetna A. Subtasks -ascertain escapement abundance by species -escapement timing by species -detenmine Yentna River contribution -oefine migrational preferences and movements -evaluate stock characteristics -evaluate mainstem spawning 8. Work Plan by Species 1. Eulachon -A eulachon study defining F-4 - - DRAFT timing, relative abundance, stock characteristics and spawning distribution Susitna River mainstem should be initiated. Gill netting will be conducted in the marine estuary and a representative sample will be collected for age, length a.nd sex analysis. An electroshocker equipped boat will be operated on the Susitna River mainstein to define spawning locations and range. Operating period will be from 7 May to 15 June. 2. Bering Cisco -Escapement monitoring on this species will be conducted at Susitna, Yentna and Sunshine stations in conjuction with salmon monitoring operations. Gear used for sampling will be SSS counters and fishwheels. Additionally, the lower east bank fishwheel at Sunshine Station will be operated beyond the close of salmon escapement monitoring program through 25 September to insure full coverage of the cisco migration. 3. Chinook Salmon - A tagging operation through the use of fishwheels, will be conducted from 7 June to 20 July at Sunshine Station. All chinook salmon intercepted in the fishwheels will be tagged with Petersen disc tags and released. A population estimate will be mad~ based on the number Of r, F-5 '--'----------------L • DRAFT upstream recoveries at upriver sampling stations and by grounds surveys of selected, upstreall spawning tributaries conducted frona late July through mid August. 4. Sockeye, Pink, Chum and Coho Salmon a. MainstetD Escapement Monitoring -Escapement aonftoring will be conducted at Susitna, Yentna and Sunshine stations. Side scan sonar (SSS) counters will be used to assess abundance and timing. Fishwheels will be employed to provide a representative sample for age, length and sex analysis. Susitna and Yentna stations will be operated from 1 July through 5 September approximately. Side Scan Sonar counters will operate at Sunshine Station from 1 July to 15 September. b. Mainstem Spawning Surveys -Two mobile electro- fishing crews will survey the '1118instem for spawning activity from 1 August to early October, and collect habitat and resident fish data. C. Electrofishing surveys to determine mainstem spawning by anadromous adults will be coordinated by the Anadromous Adult Project Leader during the salmon spawning period. F-6 • • DRAFT II. Talkeetna to Devil Canyon A. Subtasks -assess escapement abundance by species -escapement timing by species -define migrational behavior -evaluate milling activity -detennine extent of mainstem spawning -locate and survey spawning areas such as sloughs. side channels. and tributary confluence areas that may be impacted by hydro-electric development. B. Work Plan by Species 1. Chinook Salmon a. Escapement Monitoring -Chinook salmon will be intercepted by fishwheels at Talkeetna and Curry stations from 7 June to 15 July. These fish will be tagged with Petersen disc tags and released for population estimate sampling. Tag recoveries will be made on spawning ground surveys conducted on known chinook salmon spawning tributaries from late July through mid August. The AA Project leader will conduct aerial surveys of chinook salmon spawning areas in tributaries of the Susitna River between Devil Canyon and the Chulitna- Susitna-Talkeetna river confluence beginning in July and F-7 r· [ [ [ [ [ [ [ [ [ [ [ [ [ r~ [ [ [ , • • DRAFT extending through mid Au9ust. Acres American, Inc. helicopter support will be utilized and sufficient hours will be requested to survey selected tributaries between Cook Inlet and the Talkeetna River if survey data normally collected by the Region II ADF&G Sport Fish Division and shared with the Su Hydro project is not collected. A heli- copter with adequate visibility for survey work will be required. b. Migrational Movements -Approximately sixteen chinook salmon will be radio tagged with internal transmitters at Talkeetna and Curry stations and their movements monitored throughout their use of the Susitna River mainstem. 2. Sockeye, Pink, Chum and Coho Salmon a. Mainstem Escapement Monitoring -Population estimates will be secured by SSS counters and tag/release operations. Talkeetna Station crew will operate SSS ~ounters from 7 July to 15 September. Fishwheels will be opera ... : ,. from 7 June to 15 September. Curry Station ~~till use two fishwheels to monitor escapement from 10 June to 21 September. b. Migrational Movements -Approximately sixteen chum and sixteen coho salmon will be radio tagged at Talkeetna and Curry stations.· The movements of these fish will be monitored nearly daily during their migration time in the [ [ l-, L [ [ [ [ [ [ [ [ [ [ f~ [ [ [ .. ,.. -----'-----[ mainstem. • DRAFT c. Mainstem Sp4wnin~ Surveys -One crew will electroshock the Susitna River mainstem from Talkeetna to Devil Canyon for mainstem salmon spawning from 1 August to early October, and collect habitat and resident fish data. d. Spawning Ground Surveys -One crew will be stationed at Gold Creek from l August to 7 October and will survey all sloughs and tributary confluence areas for spawning activity and tag recovery of fish tagged at Talkeetna and Curry stations. This crew will also deploy set nets weekly at the entrance to Devil Canyon for the purpose of ascertaining adult salmon milling activity from 7 August to 7 September. 3. Resident Fish a. Activities related to the radio tagging of resident fish are covered by the AA Project budget excluding personal services. Personal services are provided by the RJ electro-• fishing crew. Resident tagging and trading activities will be conducted in the fall, winter and spring seasons of FY-83. F-9 !' r~ [ ATTACHMENT F-1 • L ADULT ANADROMOUS Line 100 -Personal Services by Subelement [ Subelement Staffing (MM & Class) Cost - Eulachon 4na FB I 12.6 [ Bering Cisco liTID FBI 3.2 Su Station 3111'11 FB I 9.5 21Jin FT II (w/o OT) 4.7 [-,, Yentna Station 7111'11 FB I 22.4 2.5 Rill FT II 8.0 _, Sunshine Station (Sonar} 81111l FBI 25.2 31!111 FTII 9.6 [ Sunshine Station (T/R) 3111ft FB I 9.5 411111 FTII 12.8 Mainstem Spawning Survey 7mm FBI 22.1 [ Talkeetna Station (Sonar) 10.5 nm FB I 33.1 Talkeetna Station (T/R) 5m FBI 15.8 8111n FTII 25.6 Curry (T/R) 4.5 111D F8 I 14.2 [ 4 IIIII FT Il 12.8 Gold Creek Survey 3.5 nrn FB I 11.1 • 3nm FTII 9.6 c Mainstem Spawning Survey 3.5 mm FB I 11.1 Radio Telemetry (Adult Salmon 10 !Tm FB I 31.5 Radio Telemetry (Resident Fish} 0 0 AA Administration 11 nm FB II 34.7 [ 10m FB I 31.5 9nm FTII 28.8 Stock Separation 12 11111 FB II 44.2 [ 6nm FBI 19.3 6mm FTII 2.2.4 485.3 Line 200 -Travel [ Eulachon 0.4 [ Bering Cisco 0.1 Su Station 0.3 Yentna Station 0.4 Susnhine Station Sonar (SS) 0.5 L Sunshine Station Tag & Recovery {T/R) 0.4 Mainstem Spawning Survey 0.5 Talkeetna Station (SS) 0.4 [ Talkeetna Station (T/R) 0.5 Curry (T/R) 0.2 Gold Creek Survey 0.3 Mainstem Spawnini Survey 0 .• 3 [ Radio Telemetry Adult Salmon) 0.7 Radio Telemetry (Resident fish) 0.1 AA Administration 1.0 [ Stock Separation 5.8 17.9 F-10 [ & ATTACHMENT F-1 CONTINUED line 300 -Contractual Services Eulachon 1.7 Bering Cisco 0.0 Su Station 0~9 Yentna Station 6:o SunsbfM Station (SS) 5.4 Sunshine Station (T/R) 7.2 Mainstem S~ing ~urvey 1.8 Talkeetna Station (SS) 5.1 Talkeetna Station (T/R) 3.3 Curry (T/R) 1.8 Gold Creek Survey 1.1 Mainsteam Spawning Survey 1.0 Radio Telemetry (Adult Salmn} 12.2 Radio Telemetry (Resident Fish) 4.5 AA Administration 8.0 Stock Separation U.4 91.4 line 400 -Commodities ] • Eulachon 6.1 Bering Cisco 1.1 ~ Su Station 3.9 ~ Yentna Station 9.8 Sunshine Station (SS) 7.9 .. Sunshine Station {T/R) 7.8 Mainstem Spawning Survey 10.3 .., Talkeetna Station (SS) 7.7 Talkeetna Station (T/R} 10.3 .. Curry (T/R} 7.8 ... Gold Creek Survey 9.9 Mainst~ Spawning Survey 5.2 ~ Radio Telemetry (Adult Salmon) 12.6 Radio Telemetry (Resident Fish) 0.8 ' AA Administration 8.6 Stock Separation 12.7 ,j l2'l3 1 Grand Total ..!J' (line 100 -400) 717.1 ... A • J' F-11 ,. .[ r·, __ , • ATTACHMENT F-2 [~, ADULT ANADROMOUS FISHERIES PROJECT [ Line 500 -Eq~ipment to be purchased through Acres American, Int. c Item Quantity Description Urdt Cost Total Cost - 1 7 Tent 0.3 2.1 u 2 1 Spillsbur,y base radio 2.1 2.1 3 6 Outboard engine, 25 h.p. 1.2 7.2 r' L 4 4 Refrigerator. propane 0.6 2.4 5 2 Compressor, air 0.5 1.0 [ 6 3 Outboard lift o.s 1.5 [ 7 2 Chainsaw 0.4 0.8 • 8 2 Riverboat (20') w/console 3.0 6.0 c 9 1 Boat trailer 1.2 1.2 10 3 Outboard engines, 75 h.p. 3.0 9.0 [ 11 3 Jet unit 1.0 3 .. 0 12 1 Generator, electric 0 .. 9 0.9 [ 13 1 Refrigerator 0.5 0.5 [ 14 1 Radio, conm_unication 2.0 2.0 15 20 Radio fish tags 0.1 2.0 [ 16 1 Trucks, 4 X 4 (used) 8.0 8.0 17 4 Firearm 0.3 1.2 r~ L 18 2 Egg pump, gas 0.4 0.8 19 1 Electroshocker 3.0 3.0 [ 20 2 Avon rafts, 12' 1.7 3.4 • 21 2 Outboard engines, 7.5 h.p. 0.7 [ 1.4 59.5 "[ t F-12 r[ '"" t ..... ,w k " ~ -' k' I~ ..l ., ~. ' J l· J l· J .. J •-' j ... J fill I AD L E g f.-.OAGAtilZAI..IJUL PROJECTLEADER F8 111/PCN 1721 I ASSISTANT PROJECT LEADER F8 11 I PCN 1768 r-------------------- ,, ' • . .. Radlo Telemetry I r Operations Control Leader FBI/Crew leader/PCN 1770 FBI/PCH fR 1/PCN 1769 - Exptditor FTli/PCN 1194 r--------------------,--------------------,~----- Yentna Station Sonar FBI/crew l.eader/ PCN 1392 FR 1/PCtl 1766 FTII/PCN FBI/PCN .::::: Sunsh1ne Station Sonar FBI/crew leader/ PCN FBI/ PCN FT II/ PCtf1'TJ2 Tal&eetna Statton SQnar FBI/cr~w leader PCH 1340 FRI/PCN 1424 FUIJPCN 4212 F01/PCN r----------.---------· 1 I F.ultcoon FR ftcrew ht~der PCH 1170 FBI/PC,_ 1769 Bering Chc:o FBI/crew leader PCN ' Talkeetna .. Gold Creek Survey Fllt/PCH 1796 FBI/PCN 4216 Sus itna Electroshock FBI/PCN 4181 T Sunshtne Electroshoc.k FB 1/PCN 4213 Electroshock FT 11/PCN 1788 r-------------------------~--------~---- Sunsh1ne Statton Tag/R.ecapture FTII/crew leader/ PCN1793 FBl/PCN FTJ 1/ PCNl'T96 Talkeetna Station Tag/Recapture FBI/crew leeder/PCN FT li/PCH 1793 FT tl/PCN Curry Station Tag/Recapture ~Bl/crew lttder/PCN FT 11/PCN 1795 ~ 5 eli ~; I! ~ ,., q j ' I ... ATTAClltENT F-4 STOCK SEPARATI,ON BUDGET PROPOSAL -FY83 ADULT ANADROMGUS ·fiSHERiEs PROJECT ALASKA DEPARTMENT Of FISH AND GN~E SUSITNA HYDRO· AQUATIC STUDIES Submitted by Katherine Rowell F-14 ·[ [~ [ [ [ L r· l L, [ [ [ [ [ [ • [ [ [ [ L ; ..i[ '.rbe Susitna River drainage is the largest vat:ersb!d in the Cook Inlet basin and is cmsi.dered the greatest salmon producing system in the Opper Inlet area. Therefore, the effect of tbe prc:lpOSed Sositna River dam upm the DJ;p!r Cook Inlet fishery resource is of concern. With the exceptian of sockeye and chinook salmon, quantitative assessment of the Susitna River contribution to the ccmnerci.al fishery is tnknown, due to the high number of intradrainage spawning and rearing areas, deficiency of data concerning other known and sUS{:eCted salmon producing systems in Opper Cook Inlet and the overlap in migration timing of mi'Ced stocks and species in Cook Inlet harvest areas. '.ftJe Adult Anadrauous Progr;:m of the Susitna Hydroelectric Assessment project has been examin.i.ng atundance and spnming areas of salmon within the Sl.'sitna River drainage. However, the importance of Susitna River chum, coho and pink salmon to the comercial fishery, relative to other Ug?er Cook Inlet river systems needs to be addressed. The Opper Cook Inlet commercial fishery harvests five species of Pacific salmon migrating to freshwater systems north of Anchor Point. The economically most important species is sockeye sal.mal followed in ranking order by chum, coho, pink and chinook salmon. About 747 set net and 547 drift net ~t hol.Oers participate in the atmercial fisb!ry and harvest an average of 2.8 million fish, annually worth approximately 17.9 million dollars. Susi tna River chinook salmon usually pass through the fishing area prior to the season's opening date, resulting in low commercial exploitation. As a result, stock contribltion of SUsitna River chinook to the comnercial fishery F-15 - - - is not a concern at this time. COntribution of SUSit:na River sockeye salmnn to the commercial fishery is currently being assessed through a stock separation program vbicb vas implemented in Upper Cook Inlet in 1978. 'Dle plqX)&I! of the sockeye progr• bas been to estimate tbe stock composition of the COBDercial harvest and the season• s return to each major river system. Estimates for the 1979 ard 1980 seasms show contribution of the SUSitna River at 29.7\ and 19.2\ respectively .. 'l'he success of a stock identification program requires identification of major ~ river syst.aus, knowledge of biological characteristics of adult r etur~ (abundance, timi;ng~ age struct.u~e l , and a feasibility analysis regarding use of stock identification teehni~s which will identifY ,a fi~ to its river of origin. This basel~ data is limited or noneXistent ffir cobo, pink and cbulll salmr:n stocks in Ogler Cook Inlet and is necessary to determine tbe scope and develcpment of programs to identify the c:ontril:xltioo of SUsitna River stocks to the Opper Cook Inlet fisbety. 'lbe objectives of tbis prQFOsal are to: 1. Identify major coho, chum, and pink sa.1moo producing systems in Opper Cook Inlet; 2. Determine biological characteristics (age, size, timing} of adllt returns to major prodlcing systaus; 3. Examine the feasibility of utilizing stock identification techniques to identify stock: specific characteristics for ea.ch species. F-16 [ [ [ [ [ [ [ [ [ [ [ [ [ [ ,~ L [ [ • I. Identificatioo of Major Prcd:Jctia1 Areas by Aerial survey. To detemine the .importance of one river system upon a mixed stock fishery for a given species, all other major producing SyStems must be considered. Data regarding species presence and abundance for chum, coho and pink salmon in Upper Cook Inlet river systems is limited. Though the Susitna River is considered the primary producer of coho, chlln and pink salmon in Upper Cook Inlet, these salmon species have bef:m documented in several other large river systans. Run magnitooe and timing of these systems is unknQWil particularly ,. for several large systems on the Inlet's west side. It is p:>ssible that lew abundance estimates or run timing may eliminate river systems from consideration. Bc:wever, should proc:i.lction of these systems prove significant and not be considered in a stock identification program, then stock composition estimates for other river systems, i..ncl.llding the Susitna River will be misinterpreted. Sockeye, chinook and some coho salmon escapements are monitored in Upper Cook Inlet by weir, aerial or ground surveys, and sonar counters. Systems where chum, coho and pink salmon have been documented, rut where escapements are presently not monitored will be surveyed by air beginning in July. In glacial systems, where visual assessment is imJ;;ossible clearWater tribltari.es will be surveyed to detecnine S{:eCies presence and if possible, relative abundance. This effort will emphasize west side Cook Inlet rivers and will erlcomp!SS 10-15 systems as selected by size and previous documentation of species F-17 • presence. Effort will coordinated witb projects directed by Alaska Deplrt:ment of Fisb and Game, and other organizations conduct:.ing fish assessment studies iD tJi:per Cook IDlet.. n. Escapement Sampl :ing To de1:et1Dim biological characteristics of chum, coho and pink rms into major ptoci1cing systems, escapements will be sampled for weight, length and with the exception of pink salmon, age. Escapement sampling programs currently existing for sockeye salmon will be expanded where applicable to include other species. In river systems where an established sampling regime does not exist, three two-man crews stationed in Soldotna will be flown by fixed wing aircraft and in some cases helicopter, into areas where fish have been obseived. Escapements will be sampled by beach seine and carcass surveys (foot or rubber raft). A miniJJQD ·of 500 samples are needed for each species £rem each system.. 'l'o obtain an adequate representation of age structure, river systeDs will be sampled twice cilring the season, where JX)SSible. In. Stock Identification Feasi.bi.lity Studies '[ [ [ [ c L [ [ [ r L c [ [ [ L [ [ When biological characteristics of salmon returns have been determined, necessity and feasibility of applying stock identification techniques to the runs should be examined. '1'be applicability and success of these techniques L --l F-18 e differs by ~ies. Past studies indicate that coho stocks may be identified to river of origin by scale pattern analysis and by size. Qwm salmon studies bave been attempted With use of sc:ale pattern analysis and electrophoresis. Effective pink saJmm studies have been perfomed with electrophoresis and size data. Prior to. implementing a stock identificatim program by species, the potential of a given t:ecbnique must be tested to both identify a known sample to the river of origin and to detect differences between river systems. Run timing, size and with exception of pink salmon, scale measurement data will be examined ft.'!: stock sp!Cific differences for each species. Scales will be digiti."red in the statewide stock separation lab and analyzed with size and age data using the University of Alaska CCIIIftiter system and Vectorqraphics • • minicanputer • Electrophoresis studies of pink salmon will require 100 tissue samples (eye, heart, liver and white muscle frcm each fish) fran each system and two sets of 100 samples each from two distant tributaries on the Susitna River. 'lhese .;ampl.es may be collected during escapement sampling for age,· weight and length. Tissue samples will be frozen and transp::>rted to the University of Alaska, Southeast ·for processing by the Fisheries Department (proposal attached) • F-19 • ~ "' ~ ' "! ~ _. .., ~i "" • STOCK -SEPARATION PROGRAM COSTS .. FY8J I. II. Aerial Surveys line 100 line 200 Line 300 Aircharter 80 hrs. @ 165/hr Subtotal Line 400 Line 500 Total Sub-program Escapement Sampling line 100 6 mm FBI (Soldotna scale) 6 mm FTII (Soldotna scale) Subtotal Line 200 line 300 Aircharter 50 hrs. @ 165/hr. Helicopter 10 hrs. @ 450/hr. Equipment Maintenance Contigency Subtotal line 400 Food 200/day @ SIS/day Gas 10 barrels @ $150/barrell Field Supplies. Equipment Sampling Nets & Scales Contigency · line 500 Avon 12 ft. rafts 2 Outboards 7.5 h.p. 2 Shotguns -3 Tents -3 Subtotal Subtotal Total Sub-program III. Data Analysis and Administration Line 100 12 mm FBI I F-?.0 s 0.0 s 0.0 $13.2 $13.2 0 .. 0 s 0 .. 0 $13.2 $19.3 S22.4 $41.7 s 0.0 s 8.2 s 4.5 s 1.0 s 1.5 $15.2 s 3.0 s 1.5 $ 3.7 s 2.0 s 1.0 $11.2 s 3.4 s 1.5 $ 0.9 $ 0.9 s 6.7 $74.8 $44.2 line 200 Travel Per Diem line 300 Computer lease 6 mo./180 mo .. University tJf Alt .. Cuputer charges Office Space (Soldotna) Elettroplorests Contract Shipping Vehicle Rental Contigency Subtotal line 400 Office & Computer Supplies Subtotal Total Sub-Program Grand Total All Programs F-21 s 5.8 s 1.1 s 1.0 s 1.0 s 9.6 s 0.5 s 1.0 s 2.0 $16.2 s 1..5 $ 1.5 $67.7 $155.7 .[ r; [I [ f' L: r~ [ [ [ ·~-[ ., / -· ¥ # ... ,( ..... Principal. InveStigator: Endorsement:s: S~~it;na I!YdrCH:rlect:ric: Project;, AnadrOIIICIUs .W~t sttia:it~s · Coaaercial. Pi.sb Division ...Z~.ka l>e~t: · oi Fish· and Game Anchorage., AlaSka A.J. Gharret:t: As~~i.af7 Profe~sor Universit;g of Alaska Junea~, A!aska (907}789-2101 .. ?1tftt .J~ . Dean, $cbool of Fisheries and Sciences Universit:g of Alaska, Jimeau Services Q.il i /'L .· . I '·'• l_«lf'" .. " ... _ ... ..,,t ....... / . ( L'tv;,:.-. 1\tA eJ • ._4 £... ~ .... ~ ,«,· .. ~ llich.ald Paradise · } C!( fl. RUssell Jones Vice-Chimceilor/Academi.c: Affairs Chancellor University of Alaska, Junea~ • • • Hethods for deterinin.ing t:be scream of org~n of salmonids caught: in saltwater or even in t:be main sueam of a large S!:1St:em ~e present:ly being developed. Scale ~t:t:ern anal !:Isis has proven q&tit:e useful. for several species, ncn;ab.ly soclceye salmon {Oncorhynchus nerJca) in Cook Inlet: {Betbe and JCrasnowsk.i 1979). Starch gel elect:ropho.resis is anot:her approach to st:ocJc separation which has proved. successful (see eg. Campton 1978; Grant et: al. 1980, ltilner et: al .. 1:981). Scale pat:tern anal!:lsis depends on t:he patt:ern of scale deposition which reflects t:be environment:al experiences of individuals. Those t:hat have shared similar experiences, such as fish of the same age and stock, will have sim:Har pat: terns. Starch gel elecuopboresis, on the ot:ber hand, involves the examination of genetic char.act:erist:ics a£ the stocks involved. Stacks between which litt:le gene flow e:xist:s often diverge from one another in genetic composition over long periods o£ time. This divergence is a result: of random (genetic drift:} or other processes. The genetic composit:!ons of stocks are inherited so are permanent characteristics. This has been demonstrated Ear si:x generations of pink salmon (McGregor, thesis in preparation} • Stocks a£ pink salmon (Qncorhyncbus gorbuscha) have not proved to be readily separable using scale pattern analysis because there is relatively litt:le information available on scales o£ a two year old fish and the fish appear to share remarkabl!:1 similar envirmuaental e.xperiences. There is no intent in this proposal to imply th.iJt bioc;hem.ical genetic studies provide a panacea to stack separation problelll$. The technique can work in some instances and not in others. The kinds of situations where it wil.J. not work are ones i.n which considerable gene flow (straiJing) occurs among the population examined .. [ [ L r": L [ [ r L f~ L [ [ [ [ [ [ F-23 ~-L • exhibit Olllg .Wn:iml genetic d.ivergence not enough Lor stock separation -~· -- differences fro~~~ the nortbe.z:n (.Juneau} samples. Studies of pink Sal.ltiCIIl stocks oL ICocliaJc Island (Jolmscm 1979} i.n and frollf PriDCe fii11iam Sound (Seeb and fiisbard ,1977 J indicate SOllie bet:ezogeneit:y in t:be genetic compositions, but: there is no obvious pat:t:eJ::D t:o t:be variability that might be usetlzl for st:ocJr. separation. These studies do • indicate t:bat: substant:ia:l variabi.li t:y does exist: JUDOng pink salmon st:ocks over geograpbi.cal areas larger t:.ban t:bat: ez~ bg IICGregor in the Juneau area. It: is poss:sible that upper Cook Inlet: stocJr.s are dis- 1· t:ributed over a large enough geographical. area that enough genetic divergence bas taJcen place ;ua;mg st:ocks, or at least systems, to be useful for stock identification. The goal of t:bis project is to determine whether or not: sufficient: genetic heterogeneity exists among upper Coole Inlet: pink sal.mon stocks to be ase.ful for distinguishing stocks of Susit:na River pink sa.lmon from st:ocJr.s of ot:ber upper Coole Inlet: sg:~Ds5. If sufficieDt bet:erogeneit:g exists, subsequent: work can be done to est;abJ.ish baselines that: may be used for determining the conuibution of Susi tna River piiJJc sal.mpn to • " ·~·. ---·--~--"------ • To do this, we propose t:o examine a limited ~~r of samples collected frOJII stocks of Cook Inlet: t:hat: would indi;cat;e the .e.rtenf t:~f diversity in t:he upper Inl.et:. These wou.Id be samples representing as broad a geographical range and time of return as possible. This project would only be a feasibility study, not a thorough baseline det:erm.ination. Goals 1. Using standard biochemical genetic tecbniques (eg. Barris and • Hopkinson 1976), col.2ect:ions of tissues sa.llipled from each of six different pink .sa.lzzln stocks in Cook. Inlet .wi.ll be assayed. 2. nau from t:hese coll'ections will be analyzed U) determine the feasibility of using elect:rophoresis as a stock indentificat:ion tool. Research Plan In summer of 1982, ADF and G will collect tissue samples (beart:, 111USC1e, eyes, and liver) f:r:om 100 adults em spawning grounds at each of six different locations around Cook Inlet:. Although tbe exact: location will depend where Alit' and G personnel are deployed., we suggest one Kenai • River stock, two Susit:na River stoc:JCs (as far apart as possible), and three other col:lect:ions from t:he west side of cook Inlet (spr,~r:d over the entire geographical range, say from Chi.ni t:na Bay north) • Tissues L [ [ [ f-, L [ [ [ [ [ [ L [ [ • £rom one individual should be placed in a single ziploc bag or wbirlpak aDd J>Uc:ed on ice irz acli.ate.l;. 2'.be9 sboald be kept on ice .ao .ore tblm 18 hours btdore deep LreezUJg. Lellgt:b and .-. iDtorJMt:.ioa on 8taC'b ...,.Ze .. , be useful. 2"be da~e and loc::atiDil of the .sample sbauld be • • ... · blnre been infoz:med tha~ tbis Jd.nd a£ .s.ulpLing effort .. !1 .not ag.Un be possi.ble; t:berldore, it: III!OUl.cf be advisable to .ue .a .-acb .are thorough saapling dfort in case t:be pilot. .stud!l varrenu .a follOtl up. Of course, IIIUllgsis of these sa~aples would no~ be par1; o£ tbl,! scope of Upon delivery t:o our laboratory, we will perform electrophOretic assags of tbe tissue samples. For piD1c sallrlo.rJ we are presently collect:ing dat:a on approzi.aat:elg 25 elec:t:ropboretic loci. Of these, eight e.rh.fb.it: Reporting At: tbe time the samples will be provided, late .swamer and earlg fall o:£ l982, this lab bas ot:.ber obligations it must also .meet:. tie can, however, CC~Z~Plet:e t:be analysis and report: on our findings by a:/.4- February 1983, Mt wiU submit prelimna:rt.~ report:.s as data ~-available • f-26 Justification of Budget for proposed research tbc:trouglily exam.ine 100 saap~es. It Will take app.ro%iJately o.ae week for tbe PZ to enter IIDd to &Oilly.ze tbe data and 9repare tbe final report. Supply cost for running tbe gels and all tbe biocbeaical stains is about: $4 per fish. I will. be able t:o store no 1110re t:ban t:be ~r of samples I tdll run. If ot:ber satrJples are c:ollect:ed .in anticipation of a lllDre through effort: -sbou.ld it: be warrenred -some additional arrangements will have t:o be made. Personnel PI (40 hours) Technicians (240 hours) Benefi t:s (staff and fri.nge} Supplies lft overhead 1090.00 3230.00 1698.00 F-27 6018.00 2400.00 8418.00 842.00 9260.00 [' [ [ [: [ [' L~ [ [J c [ [ r, [ r~ L ,[ [ [ [ _. . --. ;1 ""'1 ~ "" ""' ., e -"' ' -"' REFERENCES Betbe, II.L., and P.V. JCrasnowslci. l919. Stock separat.ion studies of Cook Inlet soCkeye su-m £Dncorhttncbfl• nerk.aJ based on scale patten IUUI2f}ll.is, 2971. .u..a Dept. of Fish and G411JB :tu. Leaf2. 280:lJl. c..pton, D.6. 2918. Genet.ic .uucture o~ sea-run cutthroat trout Jlal.:~ cl11r1d. cl11rld.J populati.on in the Puget Sound reg.ion. II.S. 'thes.is. un.tver.s.itf} of Washington. Grant, fi.S., G .. .B. lfJ.lMr, P. Krasnows/U, and 1'.11. Utter-1980. Use of Biochftlilic:al genetic v~~r.iants for lden'ti..t'it:at.tons of sot:lcef}fl sa.bll::.tn (Onr:orh;tJt:bus nerka) states .in Cook Inlet, AJask.l, .9!!1· :_. !1:.!!· !!!!_ Af!!at. ~· 37 :J236l247. Bar:r.til, B. and D.A. llopldtlson. l916., llandbook of enz!IJ*t electrophoresis .in bWIIIID genetics. American Elsf!IV'ier, N. r. Johnson, IC. l979. Genetic var..tat.ton in populat:ions of p..tn.lt salJDOn (0nocobV119buS gorbu!$CM) frma Kodiale Island; Alaska. II.S. thesis. Un.ivers.itf} of Plasllington, Seattle. lft:Gregor, A.J. II.S. Thesis in preparation, Un.ivers.itf} of Ala.sb, Juneau. llilner, G.B., D.J. TeeJ, F .11. Utter, and C.L. Burlef}. l98l. Coluabia .R.iver Stock Identiflcat.ion St.udp: Va11dat.ton o~ Genef:i.c lfe'tW. Pinal Report of Research (FriO), BOD, NfiAI'C, Seattle, IIA 91ll2. lfe.i, 11. 2917. r.utisticll and 4iJIIJ.f}si• of g«Je d.iversitf} .in subdivided J'C)pu.l.atiOiiS. Ann. BWDiln Genet. 4l:22S2.13. Seeb, J. and£. IIJ.sbard. l977. Genetic character.iut.ton of Pr.ince IIJ.Jl.ta Sound pink sa.lliOIJ populations. Report to Alaska De-part::ment of Fish and Game• F-28 "