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HomeMy WebLinkAboutSuWa289sec9-7Alaska Resources Library & Information Services  Susitna‐Watana Hydroelectric Project Document  ARLIS Uniform Cover Page  Title:   Salmon escapement study, Study plan Section 9.7, Study Completion Report SuWa 289  Author(s) – Personal:     Author(s) – Corporate:    LGL Alaska Research Associates, Inc. Alaska Department of Fish and Game, Division of Sport Fish AEA‐identified category, if specified:    November 2015; Study Completion and 2014/2015 Implementation Reports AEA‐identified series, if specified:   Series (ARLIS‐assigned report number):   Susitna-Watana Hydroelectric Project document number 289   Existing numbers on document:  Published by:    [Anchorage : Alaska Energy Authority, 2015]  Date published:   October 2015 Published for:   Alaska Energy Authority Date or date range of report:    Volume and/or Part numbers:   Study plan Section 9.7  Final or Draft status, as indicated:  Document type:   Pagination:  330 pages in various pagings Related work(s):   Salmon escapement study, Study plan Section 9.7, errata to Study Completion Report (November 9, 2015) Pages added/changed by ARLIS:  Notes:  This report is dated October 2015, but it was filed with the Federal Energy Regulatory Commission (FERC) on November 9, 2015; thus the November date in reference to Study Plan appears in the title of this errata document. All reports in the Susitna‐Watana Hydroelectric Project Document series include an ARLIS‐ produced cover page and an ARLIS‐assigned number for uniformity and citability.  All reports  are posted online at http://www.arlis.org/resources/susitna‐watana/    Susitna-Watana Hydroelectric Project (FERC No. 14241) Salmon Escapement Study Study Plan Section 9.7 Study Completion Report Prepared for Alaska Energy Authority Prepared by LGL Alaska Research Associates, Inc. & Alaska Department of Fish and Game, Division of Sport Fish October 2015 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page i October 2015 TABLE OF CONTENTS 1. Introduction ....................................................................................................................... 1 2. Study Objectives................................................................................................................ 1 3. Study Area ......................................................................................................................... 2 4. Methods .............................................................................................................................. 2 4.1. Objective 1: Capture, radio-tag, and track adults of five species of Pacific salmon in the Middle and Upper Susitna River in proportion to their abundance. Capture and tag Chinook, Coho, and Pink salmon in the Lower Susitna and Yentna rivers. .................................................................................3 4.1.1. Fish Capture .....................................................................................3 4.1.2. Radio-tagging ...................................................................................5 4.1.3. Tagging Goals ..................................................................................6 4.1.4. Numbers and Size of Marked and Unmarked Fish at Selected Locations ..........................................................................................7 4.1.5. Examining Handling-Induced Changes in Behavior .....................10 4.1.6. Variances ........................................................................................11 4.2. Objective 2: Determine the migration behavior and spawning locations of radio-tagged fish in the Lower, Middle, and Upper Susitna River ..................13 4.2.1. Fixed-station Monitoring ...............................................................13 4.2.2. Aerial Telemetry Surveys ..............................................................15 4.2.3. Telemetry Data Analysis ................................................................17 4.2.4. Variances ........................................................................................20 4.3. Objective 3: Characterize adult salmon migration behavior and timing within and above Devils Canyon ................................................................................20 4.3.1. Fixed-station Monitoring ...............................................................20 4.3.2. Aerial Telemetry Surveys ..............................................................21 4.3.3. Aerial Spawner Surveys .................................................................21 4.3.4. Using Sonar to Enumerate Salmon at the Proposed Dam Site ......22 4.3.5. Variances ........................................................................................22 4.4. Objective 4: Use available technology to document salmon spawning locations in turbid water...................................................................................23 4.4.1. Sonar Equipment and Methods ......................................................23 4.4.2. Sonar Data Analysis and Reporting ...............................................24 4.4.3. Variances ........................................................................................24 4.5. Objective 5: Compare historical and current data on run timing, distribution, relative abundance, and specific locations of spawning and holding salmon ..25 4.5.1. Variances ........................................................................................25 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page ii October 2015 4.6. Objective 6: Generate counts of adult Chinook Salmon spawning in the Susitna River and its tributaries .......................................................................25 4.6.1. Variances ........................................................................................26 4.7. Objective 7: Collect tissue samples to support the Fish Genetics Study .........27 4.7.1. Variances ........................................................................................27 4.8. Objective 8: Estimate the system-wide Chinook Salmon escapement and the Coho Salmon escapement to the Susitna River above the Yentna River, and the distribution of those fish among tributaries of the Susitna River ..............27 4.8.1. Variances ........................................................................................29 5. Results .............................................................................................................................. 30 5.1. Objective 1: Capture, radio-tag, and track adults of five species of Pacific Salmon in the Middle and Upper Susitna River in proportion to their abundance. Capture and tag Chinook, Coho, and Pink salmon in the Lower Susitna and Yentna rivers. ...............................................................................30 5.1.1. Fish Capture and Fish Tagging ......................................................31 5.1.2. Numbers and Size of Marked and Unmarked Fish Recovered at Selected Locations .........................................................................35 5.1.3. Assessing Any Stock- and Size-selective Capture .........................37 5.1.4. Examining Handling-Induced Changes in Behavior .....................42 5.2. Objective 2: Determine the migration behavior and spawning locations of radio-tagged fish in the Lower, Middle, and Upper Susitna River ..................44 5.2.1. Chinook Salmon.............................................................................44 5.2.2. Chum Salmon.................................................................................47 5.2.3. Coho Salmon ..................................................................................48 5.2.4. Pink Salmon ...................................................................................50 5.2.5. Sockeye Salmon .............................................................................52 5.3. Objective 3: Characterize adult salmon migration behavior and timing within and above Devils Canyon ................................................................................53 5.3.1. Chinook Salmon.............................................................................53 5.3.2. Sockeye Salmon .............................................................................58 5.3.3. Other Species .................................................................................59 5.4. Objective 4: Use available technology to document salmon spawning locations in turbid water...................................................................................59 5.4.1. Programmatic Summary ................................................................60 5.5. Objective 6: Generate counts of adult Chinook Salmon spawning in the Susitna River and its tributaries .......................................................................60 5.5.1. Indian River Escapement Estimate ................................................61 5.5.2. Estimated Abundance of Chinook Salmon Upstream of Devils Canyon ...........................................................................................62 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page iii October 2015 5.6. Objective 7: Collect tissue samples to support the Fish Genetics Study .........63 5.7. Objective 8: Estimate the system-wide Chinook Salmon escapement and the Coho Salmon escapement to the Susitna River above the Yentna River, and the distribution of those fish among tributaries of the Susitna River ..............63 6. Discussion......................................................................................................................... 64 6.1. Chinook ............................................................................................................64 6.1.1. Timing of Migration ......................................................................64 6.1.2. Timing of Spawning ......................................................................65 6.1.3. Distribution to Mainstem and Tributaries ......................................66 6.1.4. Mainstem Habitat and Tributary Use .............................................68 6.1.5. Abundance Estimates .....................................................................70 6.2. Chum ................................................................................................................71 6.2.1. Timing of Migration ......................................................................71 6.2.2. Timing of Spawning ......................................................................71 6.2.3. Distribution to Mainstem and Tributaries ......................................72 6.2.4. Mainstem Habitat and Tributary Use .............................................73 6.2.5. Abundance Estimates .....................................................................74 6.3. Coho .................................................................................................................75 6.3.1. Timing of Migration ......................................................................75 6.3.2. Timing of Spawning ......................................................................75 6.3.3. Distribution to Mainstem and Tributaries ......................................76 6.3.4. Mainstem Habitat and Tributary Use .............................................77 6.3.5. Abundance Estimates .....................................................................78 6.4. Pink ..................................................................................................................79 6.4.1. Timing of Migration ......................................................................79 6.4.2. Timing of Spawning ......................................................................79 6.4.3. Distribution to Mainstem and Tributaries ......................................80 6.4.4. Mainstem Habitat and Tributary Use .............................................81 6.4.5. Abundance Estimates .....................................................................82 6.5. Sockeye ............................................................................................................83 6.5.1. Timing of Migration ......................................................................83 6.5.2. Timing of Spawning ......................................................................83 6.5.3. Distribution to Mainstem and Tributaries ......................................84 6.5.4. Mainstem Habitat and Tributary Use .............................................85 6.5.5. Abundance Estimates .....................................................................86 7. Conclusions ...................................................................................................................... 87 8. Literature Cited .............................................................................................................. 87 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page iv October 2015 9. Tables ............................................................................................................................... 92 10. Figures ............................................................................................................................ 114 LIST OF TABLES Table 4.3-1. Aerial spawner surveys conducted in the Middle and Upper River by location and date, 2014. .................................................................................................... 93 Table 5.1-1. Number of adult salmon radio-tagged in the Susitna River Basin from 2012 to 2014, by species, fish size, and tagging location. ......................................................... 94 Table 5.2-1. Classifications for radio-tagged salmon in 2014, by species and release location. ............................................................................................................................. 95 Table 5.2-2. The proportions of radio-tagged salmon of known destination that were detected in the Middle and Upper rivers, and that subsequently returned downstream to enter a Lower River tributary, or that appeared to have a mainstem destination in the Lower River, 2014. ............................................................................... 98 Table 5.2-3. Farthest upstream detection locations for radio-tagged fish that were eventually assigned to mainstem or tributary spawning locations downstream of Lane Station (top panel: fish released in the Lower River; bottom panel: fish released in the Middle River), 2014. ............................................................................... 100 Table 5.3-1. Number of salmon radio-tagged in the Lower and Middle rivers, and the number of radio-tagged salmon that were detected at or above the Gateway Station, above each impediment, and above the proposed dam site, 2014. .................... 101 Table 5.3-2. Details of the radio-tagged salmon that approached or passed the Middle River impediments, 2014. ............................................................................................... 102 Table 5.3-3. Destinations of radio-tagged salmon that passed each Middle River impediment, 2014. .......................................................................................................... 106 Table 5.3-4. Dates on which radio-tagged fish were first detected upstream of Impediment 3 (2012-2014), with corresponding flows as measured at Tsusena and Gold creeks. .................................................................................................................... 107 Table 5.3-5. Aerial Chinook Salmon spawning escapement surveys. Number of flights, and date and magnitude of peak counts per stream and survey year. ............................. 108 Table 5.4-1. Survey effort and observations using DIDSON to identify Chinook Salmon spawning behavior in turbid water, 2014. ....................................................................... 110 Table 6.2-1. Confirmed spawning salmon in slough habitats of the Middle River from 1981 to 1985, and confirmed spawning locations in 2012, 2013, and 2014................... 112 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page v October 2015 LIST OF FIGURES Figure 3-1. Susitna River watershed showing fish capture sites (fishwheels) and the locations of fixed-station telemetry receiver sites, 2014. ............................................... 115 Figure 4.1-1. Middle River Segment showing sites for fish capture (Site 1, PRM 124.1; Site 2, PRM 123.0; and Site 3, PRM 126.0), sonar (ARIS; PRM 124.0), Curry camp (PRM 124.2), and the Lane Creek (PRM 116.7) and ‘Gateway’ (PRM 130.1) fixed-station receiver sites, 2014. ........................................................................ 117 Figure 4.3-1. Extent of aerial spawner surveys in the Indian River and tributaries in and above Devils Canyon, 2014. ........................................................................................... 118 Figure 5.1-1. Daily discharge of the Susitna River at the Gold Creek gauge from April to November in 2012, 2013, and 2014. ............................................................................... 119 Figure 5.1-2. Daily discharge of the Susitna River at the Tsusena Creek gauge from April to November in 2012, 2013, and 2014. ................................................................. 119 Figure 5.1-3. Median travel speeds of radio-tagged fish in four major river reaches, by species. ............................................................................................................................ 120 Figure 5.2-1. Classifications for radio-tagged salmon released in the Lower River (left panels) or Middle River (right panels), by species/life history stage, 2014. .................. 121 Figure 5.2-2. Relative frequencies of tributary use by radio-tagged salmon released in the Lower River, by species, 2014. Shown as a percentage of all fish classified to a tributary destination. ....................................................................................................... 122 Figure 5.2-3. Relative frequencies of tributary use by radio-tagged salmon released in the Middle River, by species, 2014. ...................................................................................... 123 Figure 5.3-1. Daily numbers of radio-tagged large Chinook Salmon that approached and passed each of the three Middle River impediments in 2014. ........................................ 124 Figure 5.3-2. Daily numbers of radio-tagged small Chinook (top panel) and Sockeye (bottom panel) salmon that approached and passed Middle River Impediment 1 in 2014................................................................................................................................. 125 Figure 5.3-3. Daily number of radio-tagged Chinook Salmon that held below Impediment 3 in 2014. .................................................................................................... 126 Figure 5.3-4. Flows (measured at Gold Creek) in 2012, 2013 and 2014, along with median (solid black line), and the 10th and 90th percentile (dotted lines) historical flows. ............................................................................................................................... 127 Figure 5.7-1. Destinations for radio-tagged Chinook Salmon released in the Lower River in 2013. ........................................................................................................................... 128 Figure 5.7-2. Destinations for radio-tagged Chinook Salmon released in the Lower River in 2014. ........................................................................................................................... 129 Figure 5.7-3. Destinations for radio-tagged Coho Salmon released in the Lower River in 2013 (yellow circles). ...................................................................................................... 130 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page vi October 2015 Figure 5.7-4. Destinations for radio-tagged Coho Salmon released in the Lower River in 2014 (yellow circles). ...................................................................................................... 131 LIST OF APPENDICES Appendix A: Fish Capture and Tagging Table A-1. Number of salmon caught and radio-tagged at two fishwheel sites and from gillnets in the Lower River, PRM 33.4–34.2, 2014. ........................................................... 1 Table A-2. Number of Chinook Salmon caught and radio-tagged at fishwheel sites and in gillnets in the Yentna River (RM 6 and RM 18), 2014. ..................................................... 1 Table A-3. Number of salmon radio-tagged at three fishwheel sites and in gillnets in the Middle River, by size category, 2014. ................................................................................ 2 Table A-4. Number of salmon caught in the Lower River and Yentna River and their length statistics, 2014. ......................................................................................................... 3 Table A-5. Number of salmon captured at three fishwheel sites and in gillnets in the Middle River, by size category, 2014. ................................................................................ 4 Table A-6. Number of fish caught, tagged, and biosampled at the Middle River fishwheels, 2014. ................................................................................................................ 5 Table A-7. Daily fishing effort and the number of salmon caught and radio-tagged during gillnet operations in the vicinity of Curry, 2014. .................................................... 6 Table A-8. Summary of run-timing and catch information for salmon captured in fishwheels located in the Middle River near Curry, by year and species. .......................... 7 Table A-9. Comparisons between the cumulative length-frequency distributions of fish sampled in the Lower River and Yentna River using the Kolmogorov-Smirnov (KS) two-sample test, 2014................................................................................................. 8 Table A-10. Comparisons between the cumulative length-frequency distributions of fish sampled in the Middle River using the Kolmogorov-Smirnov (KS) two-sample test, 2014. ............................................................................................................................ 9 Figure A-1. Daily fishing effort (hours) at two fishwheel sites in the Lower River, 2014. ......... 10 Figure A-2. Daily gillnet effort (hours) in the Lower River, by mesh size, 2014. ...................... 10 Figure A-3. Daily fishing effort (hours) at four fishwheel sites in the Yentna River, 2014. ....... 11 Figure A-4. Daily gillnet effort (hours) during tagging (top panel) and recovery (bottom panel) operations in the Yentna River, by mesh size, 2014. ............................................. 12 Figure A-5. Daily fishing effort (hours) and rotational speed (RPM) at three fishwheel sites in the Middle River, 2014. ........................................................................................ 12 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page vii October 2015 Figure A-6. Daily number of radio tags applied to adult salmon species captured at two fishwheel sites and in gillnets in the Lower River, 2014. ................................................. 13 Figure A-7. Daily number of radio tags applied to adult salmon species captured at two fishwheel sites and in gillnets in the Yentna River (RM 6), 2014. ................................... 14 Figure A-8. Daily number of radio tags applied to adult salmon species captured at three fishwheel sites and in gillnets in the Middle River, 2014. ................................................ 15 Figure A-9. Daily catch-per-unit-effort of adult salmon species at the Lower River fishwheels, and the Susitna River discharge at Sunshine, 2014. ...................................... 16 Figure A-10. Daily catch-per-unit-effort for Chinook and Coho salmon at the Yentna River fishwheels, by site, 2014. ........................................................................................ 17 Figure A-11. Number of radio tags deployed in species of salmon at the Middle River fishwheels in 2014 relative to fishwheel catches in 2012, 2013, and 2014. ..................... 18 Figure A-12. Daily catch-per-unit-effort at the Middle River fishwheels, by species, and the Susitna River discharge at Gold Creek, 2014. ............................................................ 19 Figure A-13. Comparison of Chinook Salmon catches (top panel), relative proportion of catches (middle panel), and cumulative proportion of catches (bottom panel), at the Middle River fishwheels near Curry, by year. ............................................................ 20 Figure A-14. Comparison of Chum Salmon catches (top panel), relative proportion of catches (middle panel), and cumulative proportion of catches (bottom panel), at the Middle River fishwheels near Curry, by year. ............................................................ 21 Figure A-15. Comparison of Coho Salmon catches (top panel), relative proportion of catches (middle panel), and cumulative proportion of catches (bottom panel), at the Middle River fishwheels near Curry, by year. ............................................................ 22 Figure A-16. Comparison of Pink Salmon catches (top panel), relative proportion of catches (middle panel), and cumulative proportion of catches (bottom panel), at the Middle River fishwheels near Curry, by year. ............................................................ 23 Figure A-17. Comparison of Sockeye Salmon catches (top panel), relative proportion of catches (middle panel), and cumulative proportion of catches (bottom panel), at the Middle River fishwheels near Curry, by year. ............................................................ 24 Figure A-18. Daily sampling effort, and the amount of imagery reviewed (review effort), for an ARIS sonar unit operated immediately downstream of the fishwheel at Site 1 in the Middle River, 2014. ............................................................................................. 25 Figure A-19. Catch-per-unit-effort, or the number of targets counted per hour of imagery reviewed, on the ARIS unit located immediately downstream of the Site 1 fishwheel, 2014. ................................................................................................................ 25 Figure A-20. Comparison of the catch-per-unit-effort of adult salmon at the Site 1 fishwheel and concurrent net upstream counts of fish on the ARIS unit located immediately downstream of the fishwheel, 2014. ............................................................ 26 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page viii October 2015 Figure A-21. Relative percentage of fish counted using ARIS at Site 1 as a function of the distance where they were first detected in the field of view, by time period, 2014................................................................................................................................... 26 Figure A-22. Diel migration of upstream-moving fish counted using ARIS at Site 1, by size category and time period, 2014. ................................................................................ 27 Figure A-23. Cumulative length-frequency distributions for Chinook Salmon captured in the Lower River, by capture site, 2014. ............................................................................ 28 Figure A-24. Cumulative length-frequency distributions for Chinook and Pink salmon caught and radio-tagged in the Lower River, by species, 2014. ....................................... 28 Figure A-25. Cumulative length-frequency distributions for Chinook Salmon radio- tagged in the Lower River and inspected and recaptured at the Deshka River and Montana Creek weir sites, 2014........................................................................................ 29 Figure A-26. Cumulative length-frequency distributions for Chinook Salmon captured at RM 6 (left panel) and RM 18 (right panel) in the Yentna River, 2014. ........................... 29 Figure A-27. Cumulative length-frequency distributions for Chinook Salmon measuring 50 cm METF or greater that were caught and dart-tagged in the Yentna River (RM 6), 2014. .................................................................................................................... 30 Figure A-28. Cumulative length-frequency distributions for Chinook Salmon dart-tagged at Yentna RM 6 and inspected and recaptured at Yentna RM 18 (fishwheels and gillnets combined), 2014. .................................................................................................. 30 Figure A-29. Cumulative length-frequency distributions for salmon captured in the Middle River fishwheels, by species and capture site, 2014. ........................................... 31 Figure A-30. Cumulative length-frequency distributions for salmon caught and radio- tagged in the Middle River, by species, 2014. .................................................................. 32 Appendix B: Daily Fish Passage at Weir and Sonar Sites in the Lower and Middle Rivers Table B-1. Deshka River weir daily passage rates and tag recaptures, by species, 2014. ............. 1 Table B-2. Montana Creek weir daily passage rates and tag recaptures, by species, 2014. .......... 5 Table B-3. Length statistics for tagged and untagged adult salmon sampled at the Deshka River and Montana Creek weirs, by species, 2014. ............................................................ 9 Table B-4. Daily amount of video imagery collected and reviewed at the Indian River weir, and the net upstream count of fish, by species, 2014. ............................................... 9 Table B-5. Daily number of Chinook Salmon inspected for tags, and the number of dart- tag recaptures, at RM 18 sites on the Yentna River, 2014. ............................................... 10 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page ix October 2015 Appendix C: Fixed-station Receiver Sites (Setup and Performance) and Mobile-tracking Survey Effort Table C-1. Location and antenna orientation of fixed-station receivers in the Susitna River drainage, 2014. .......................................................................................................... 1 Table C-2. Monitoring efficiency (percent operational) of fixed-station receivers in the Lower River Basin in 2014, by week. ................................................................................. 3 Table C-3. Monitoring efficiency (percent operational) of fixed-station receivers in the Middle and Upper River basins in 2014, by week. ............................................................. 4 Table C-4. List of the aerial telemetry surveys conducted in 2014, by location, date, and vehicle type (helicopter, fixed-wing). ................................................................................. 5 Appendix D: Spawning Destinations Table D-1. Summary of monitoring effort at potential spawning sites, by species, as part of the Habitat Suitability Criteria (HSC) component of the Fish and Aquatics Instream Flow Study (RSP Section 8.5), 2014. .................................................................. 1 Table D-2. Summary of monitoring effort at potential spawning sites for Chinook Salmon in the Middle River, 2014. ..................................................................................... 2 Table D-3. Summary of monitoring effort at potential spawning sites for Chum Salmon in the Middle River, 2014. .................................................................................................. 3 Table D-4. Summary of monitoring effort at potential spawning sites for Coho Salmon in the Middle River, 2014. ...................................................................................................... 4 Table D-5. Summary of monitoring effort at potential spawning sites for Pink Salmon in the Middle River, 2014. ...................................................................................................... 5 Table D-6. Summary of monitoring effort at potential spawning sites for Sockeye Salmon in the Middle River, 2014. ..................................................................................... 6 Table D-7. Details of impediment-passage events for radio-tagged fish, 2014. ............................ 7 Table D-8. Number of Chinook Salmon counted during aerial spawner surveys, by location and survey period, 2014. ..................................................................................... 11 Table D-9. Summary of weather variability during the adult salmon aerial spawner surveys in the Middle and Upper rivers, 2014. ................................................................. 12 Table D-10. Summary of survey condition rankings during the adult salmon aerial spawner surveys in the Middle and Upper rivers, 2014. ................................................... 12 Figure D-1. Destinations for radio-tagged Chinook Salmon released in the Lower River in 2012-2014. .................................................................................................................... 13 Figure D-2. Destinations for radio-tagged Chinook Salmon released in the Middle River in 2012-2014. .................................................................................................................... 14 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page x October 2015 Figure D-3. Destinations for radio-tagged Chum Salmon released in the Lower River in 2012................................................................................................................................... 15 Figure D-4. Destinations for radio-tagged Chum Salmon released in the Middle River in 2012-2014. ........................................................................................................................ 16 Figure D-5. Destinations for radio-tagged Coho Salmon released in the Lower River in 2012-2014. ........................................................................................................................ 17 Figure D-6. Destinations for radio-tagged Coho Salmon released in the Middle River in 2012-2014. ........................................................................................................................ 18 Figure D-7. Destinations for radio-tagged Pink Salmon released in the Lower River in 2012-2014. ........................................................................................................................ 19 Figure D-8. Destinations for radio-tagged Pink Salmon released in the Middle River in 2012-2014. ........................................................................................................................ 20 Figure D-9. Destinations for radio-tagged Sockeye Salmon released in the Lower River in 2012. ............................................................................................................................. 21 Figure D-10. Destinations for radio-tagged Sockeye Salmon released in the Middle River in 2012-2014. .................................................................................................................... 22 Figure D-11. Potential mainstem spawning sites for radio-tagged Chinook Salmon in the Lower River, PRM 40–104, 2012-2014. .......................................................................... 23 Figure D-12. Potential mainstem spawning sites for radio-tagged Chinook Salmon in the Middle River (red and yellow dots), PRM 103–157, 2012 - 2014. .................................. 24 Figure D-13. Potential mainstem spawning sites for radio-tagged Chum Salmon in the Lower River, PRM 103–157, 2012 - 2014. ...................................................................... 25 Figure D-14. Potential mainstem spawning sites for radio-tagged Chum Salmon in the northern half of the Middle River, PRM 40–104, 2012 - 2014. ....................................... 26 Figure D-15. Potential mainstem spawning sites for radio-tagged Chum Salmon in the southern half of the Middle River, PRM 103–157, 2012 -2014. ...................................... 27 Figure D-16. Potential mainstem spawning sites for radio-tagged Coho Salmon in the Lower River, PRM 40–104, 2012 - 2014. ........................................................................ 28 Figure D-17. Potential mainstem spawning sites for radio-tagged Coho Salmon in the Middle River, PRM 103–157, 2012 - 2014. ..................................................................... 29 Figure D-18. Potential mainstem spawning sites for radio-tagged Pink Salmon in the Lower River, PRM103–157, 2012 -2014. ........................................................................ 30 Figure D-19. Potential mainstem spawning sites for radio-tagged Pink Salmon in the Middle River, PRM 103–157, 2012 - 2014. ..................................................................... 31 Figure D-20. Potential mainstem spawning sites for radio-tagged Sockeye Salmon in the Middle River, PRM 103–157, 2012 - 2014. ..................................................................... 32 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page xi October 2015 Appendix E: Radio Tag Recoveries Table E-1. Radio tag recovery information for fish released in the Lower River and Yentna River, 2014. ............................................................................................................ 1 Table E-2. Radio tag recovery information for fish released in the Middle River, 2014. ............. 4 Appendix F: Tracking Histories of Chinook Salmon Above Impediment 3 Table F-1. Summary of migration and spawning behavior for radio-tagged Chinook Salmon after they passed Impediment 3, 2012–2014. ........................................................ 1 Figure F-1. Tracking history of a radio-tagged Chinook Salmon (tag #537) that was detected above Impediment 3, PRM 123–167, 2014. ......................................................... 2 Figure F-2. Tracking history of a radio-tagged Chinook Salmon (tag #787) that was detected above Impediment 3, PRM 97–245, 2014. ........................................................... 3 Figure F-3. Tracking history of a radio-tagged Chinook Salmon (tag #27) that was detected above Impediment 3, 2012. .................................................................................. 4 Figure F-4. Tracking history of a radio-tagged Chinook Salmon (tag #52) that was detected above Impediment 3, 2012. .................................................................................. 5 Figure F-5. Tracking history of a radio-tagged Chinook Salmon (tag #94) that was detected above Impediment 3, 2012. .................................................................................. 6 Figure F-6. Tracking history of a radio-tagged Chinook Salmon (tag #104) that was detected above Impediment 3, 2012. .................................................................................. 7 Figure F-7. Tracking history of a radio-tagged Chinook Salmon (tag #113) that was detected above Impediment 3, 2012. .................................................................................. 8 Figure F-8. Tracking history of a radio-tagged Chinook Salmon (tag #219) that was detected above Impediment 3, 2012. .................................................................................. 9 Figure F-9. Tracking history of a radio-tagged Chinook Salmon (tag #246) that was detected above Impediment 3, 2012. ................................................................................ 10 Figure F-10. Tracking history of a radio-tagged Chinook Salmon (tag #257) that was detected above Impediment 3, 2012. ................................................................................ 11 Figure F-11. Tracking history of a radio-tagged Chinook Salmon (tag #266) that was detected above Impediment 3, 2012. ................................................................................ 12 Figure F-12. Tracking history of a radio-tagged Chinook Salmon (tag #359) that was detected above Impediment 3, 2012. ................................................................................ 13 Figure F-13. Tracking history of a radio-tagged Chinook Salmon (tag #5005) that was detected above Impediment 3, 2012. ................................................................................ 14 Figure F-14. Tracking history of a radio-tagged Chinook Salmon (tag #5019) that was detected above Impediment 3, 2012. ................................................................................ 15 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page xii October 2015 Figure F-15. Tracking history of a radio-tagged Chinook Salmon (tag #241) that was detected above Impediment 3, 2013. ................................................................................ 16 Figure F-16. Tracking history of a radio-tagged Chinook Salmon (tag #272) that was detected above Impediment 3, 2013. ................................................................................ 17 Figure F-17. Tracking history of a radio-tagged Chinook Salmon (tag #395) that was detected above Impediment 3, 2013. ................................................................................ 18 Appendix G: Counts of Chinook Salmon at Watana Canyon Using Sonar Table G-1. Location details for Watana Canyon sonar sites near PRM 187.1 in 2014. ................ 9 Table G-2. Operating frequencies and data collection parameters used for the ARIS monitoring stations at Watana Canyon in 2014. ................................................................. 9 Table G-3. Percent coverage for each sonar station and combined based on wetted channel width, wetted edge to sonar, and ensonified range at sample sites near PRM 187.1 in Watana Canyon. .......................................................................................... 9 Table G-4. Sample effort, CPUE, and net upstream count of fish measuring 50 cm or greater at two ARIS units located at PRM 187.1 in the Upper River, 2014. .................... 10 Table G-5. Discharge estimates based on spatial integration of velocity data for ADCP surveys conducted in the Watana Canyon in August, 2014. ............................................. 12 Table G-6. Daily data collection parameters at the Watana Canyon sonar sites, 2014. .............. 13 Figure G-1. Photograph of the Susitna River immediately downstream of the proposed Watana Dam Site (PRM 187.1) showing the location of the river left and river right sonar sites and the wetted channel width. ................................................................ 15 Figure G-2. Photographs showing the ARIS mounts deployed at the left bank (left) and right bank (right) monitoring stations. .............................................................................. 15 Figure G-3. Photographs showing the environmental boxes used to house the ARIS systems electronic components, and power sources (battery banks are inside action packers) for the left bank (left) and right bank (right) monitoring stations. .......... 16 Figure G-4. Still images from ARIS data showing the cobble substrate (light-colored structure) along the left bank (left) and right bank (right) fields-of-view. ....................... 16 Figure G-5. Ortho image showing the ensonified wetted width coverage of each ARIS unit near the Watana Dam Site, 2014. .............................................................................. 17 Figure G-6. Screen shots of Right Bank ARIS data showing echograms (left) and still sonar imagery (right) for a resident fish (top) and Chinook Salmon (bottom). ................ 18 Figure G-7. Daily sampling effort at two ARIS sonar units located at PRM 187.1 in the Upper River, 2014. ............................................................................................................ 19 Figure G-8. Bathymetry profiles derived from ADCP data for transects aligned with ARIS sampling locations (PRM 187.1). ........................................................................... 20 Figure G-9. Net upstream count of fish measuring 50 cm or greater at two ARIS sonar units located at PRM 187.1 in the Upper River, 2014. ..................................................... 21 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page xiii October 2015 Figure G-10. Diel migration of fish measuring 50 cm or greater counted at two ARIS sonar units located at PRM 187.1 in the Upper River. ..................................................... 21 Figure G-11. Percent of fish measuring 50 cm or greater counted at two ARIS sonar units located at PRM 187.1 in the Upper River as a function of distance from the sonar units, 2014. .............................................................................................................. 22 Figure G-12. Series of velocity profiles collected along transects using an ADCP at Watana Canyon (PRM 187.1) in 2014. Transects are arranged from upstream (top) to downstream (bottom) to allow for best presentation of the transect-to- transect channel morphology. Range of sonar stations is shown for the River Left site at Transect 3 and River Right site at Transect 6. ........................................................ 22 Figure G-13. Individual velocity profile for Transect 3 in Watana Canyon (PRM 187.1) collected with an ADCP in 2014. This profile corresponds to the location of the sonar station on River Left (ensonified zone illustrated). ................................................. 23 Figure G-14. Individual velocity profile for Transect 6 in Watana Canyon (PRM 187.1) collected with an ADCP in 2014. This profile corresponds to the location of the sonar station on River Right (ensonified zone illustrated). ............................................... 23 Figure G-15. Bathymetry in Watana Canyon (PRM 187.1) based on seven serial ADCP transects, 2014................................................................................................................... 24 Appendix H: Chinook Salmon – Indian River Escapement Estimate for Chinook Salmon Table H-1. Number of Chinook Salmon counted during aerial spawner surveys in the Indian River, and the number of radio-tagged large Chinook Salmon detected, by tag site, 2014. ...................................................................................................................... 3 Table H-2. Summary of AUC abundance estimate, mark rate at the Middle River tag site, and the expected number of fish passed the Watana Dam sonar site, 2014. ...................... 5 Figure H-1. Number of Chinook Salmon counted during aerial spawner surveys above Bridge 1 in the Indian River, 2014. ..................................................................................... 5 Figure H-2. Relative frequency of residence times (days) for radio-tagged Chinook Salmon above Bridge 1 in the Indian River, 2014. ............................................................. 6 Appendix I: Estimate the System-wide Chinook Salmon Escapement and the Distribution of Those Fish Among Tributaries of the Susitna River Table I-1. Diagnostic tests for mark-recapture data for mainstem Susitna River Chinook Salmon measuring 50.0–78.5 cm METF, 2014. ............................................................... 11 Table I-2. Diagnostic tests for mark-recapture data for mainstem Susitna River Chinook Salmon measuring 78.5 cm METF or greater, 2014. ........................................................ 12 Table I-3. Estimated abundance, number of radio tags deployed, and relative weights (number of spawners per tag) used to estimate abundance within size stratum for STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page xiv October 2015 Chinook Salmon spawning upstream from the lower mainstem tagging site in the Susitna River, 2014. .......................................................................................................... 13 Table I-4. Chinook Salmon measuring 50 cm METF or greater spawning distributions, based on weighted abundance (Table I-3), in the mainstem Susitna River above the Lower River tagging site, 2014. .................................................................................. 13 Table I-5. Diagnostic tests for mark-recapture data for Yentna River Chinook Salmon >50 cm METF, 2014a........................................................................................................ 14 Table I-6. Chinook Salmon spawning distributions in the Yentna River above the RM 6 tagging site, 2014. ............................................................................................................. 15 Table I-7. Diagnostic tests for mark-recapture data for mainstem Susitna River Coho Salmon 40-55 cm METF, 2014a. ...................................................................................... 16 Table I-8. Diagnostic tests for mark-recapture data for mainstem Susitna River Coho Salmon >55 cm METF, 2014a. ......................................................................................... 17 Table I-9. Estimated abundance, number of radio tags deployed, and relative weights (number of spawners per tag) used to estimate abundance within size stratum for Coho Salmon spawning upstream from the lower mainstem tagging site in the Susitna River, 2014. .......................................................................................................... 18 Table I-10. Coho Salmon spawning distributions, based on weighted abundance (Table I- 3), in the mainstem Susitna River above the lower river tagging site, 2014. ................... 18 Figure I-1. Empirical cumulative distribution functions (ECDF) of length (in mm) of Chinook Salmon (METF ≥ 50 cm) marked during first event sampling at the lower mainstem Susitna River tagging site and all recaptures during second event sampling at the Deshka River and Montana Creek weirs, 2014. ........................................ 1 Figure I-2. Empirical cumulative distribution functions (ECDF) of length (in millimeters, mm) of Chinook (METF ≥ 500 mm) inspected for marks and all recaptured salmon during second event sampling at the Deshka River weir, 2014. .......... 2 Figure I-3. Empirical cumulative distribution functions (ECDF) of length (in mm) of Chinook Salmon (METF ≥ 500 mm) inspected for marks and all recaptured salmon during second event sampling at the Montana Creek weir, 2014. ......................... 3 Figure I-4. The 4-day lag between total weir count and radio-tagged Chinook Salmon at the Deshka River weir, 2014. .............................................................................................. 4 Figure I-5. Empirical cumulative distribution functions of METF length (mm) of all Chinook Salmon (> 500 mm) marked during first event at the lower Yentna River tagging site at RM 6 and of all salmon recaptured during second event sampling at RM 18 of the lower Yentna River, 2014. ............................................................................ 5 Figure I-6. Empirical cumulative distribution functions of METF length (mm) of Chinook Salmon (> 500 mm) inspected for marks during second event sampling at the Yentna RM 18 east fishwheel, and all salmon recaptured during inspection in 2014. ............................................................................................................................... 6 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page xv October 2015 Figure I-7. Empirical cumulative distribution functions of METF length (mm) of Chinook Salmon (>500 mm) inspected for marks during second event sampling at the Yentna RM 18 west fishwheel, and all salmon recaptured during inspection in 2014..................................................................................................................................... 7 Figure I-8. Empirical cumulative distribution functions of METF length (mm) of Chinook Salmon (>500 mm) inspected for marks during second event sampling (pooled data) and all salmon recaptured during inspection at Yentna RM 18 in 2014..................................................................................................................................... 8 Figure I-9. Timing at Yentna River second event fishwheels (RM 18) of all fish caught >500 mm METF and of recaptures. .................................................................................... 9 Figure I-10. Empirical cumulative distribution functions of METF length (mm) of all Coho Salmon (> 400 mm) marked during first event at the lower mainstem Susitna River tagging site and of all salmon recaptured during second event sampling at the Deshka River and Montana Creek weirs during second event, 2014................................................................................................................................... 10 Figure I-11. Empirical cumulative distribution functions of METF length (mm) of Coho Salmon (> 400 mm) inspected for marks during second event sampling at the Deshka River weir, 2014 and of all salmon recaptured during inspection. ...................... 11 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page xvi October 2015 LIST OF ACRONYMS, ABBREVIATIONS, AND DEFINITIONS Abbreviation Definition ADCP acoustic Doppler current profiler ADF&G Alaska Department of Fish and Game AEA Alaska Energy Authority ARIS Adaptive Resolution Imaging Sonar ATS Advanced Telemetry Systems, Inc. AUC area under the curve cfs cubic feet per second cm Centimeter CM Complete Mixing (test) CPUE catch per unit effort DIDSON Dual Frequency Identification Sonar ECDF Empirical cumulative distribution function EP Equal Proportion (test) FERC Federal Energy Regulatory Commission FOV field-of-view ft Feet ft/s feet per second g Gram GIS geographic information system GPS global positioning system HSC Habitat Suitability Criteria ILP Integrated Licensing Process in Inch ISR Initial Study Report km Kilometer KS Kolmogorov-Smirnov m Meter mi Mile m/s meters per second METF mid-eye to fork MHz Megahertz mm Millimeter NTU nephelometric turbidity units STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page xvii October 2015 Abbreviation Definition oz Ounce PRM Project River Mile RM river mile RPM revolutions per minute RSP Revised Study Plan SMC Sound Metrics Corporation SPD study plan determination TL total length USGS United States Geological Survey W Watt V Volt STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 1 October 2015 1. INTRODUCTION This Salmon Escapement Study, Section 9.7 of the Revised Study Plan (RSP) approved by the Federal Energy Regulatory Commission (FERC or Commission) for the Susitna-Watana Hydroelectric Project, FERC Project No. 14241, focuses on characterizing the current distribution, abundance, habitat use, and migratory behavior of all species of adult anadromous salmon (Oncorhynchus spp.) across mainstem river habitats and select tributaries above the Three Rivers Confluence (i.e., confluence of the Susitna, Chulitna, and Talkeetna rivers). A summary of the development of this study, together with the Alaska Energy Authority’s (AEA) implementation of it through the 2013 study season, appears in Part A, Section 1 of the Initial Study Report (ISR) filed with FERC in June 2014. As required under FERC’s regulations for the Integrated Licensing Process (ILP), the ISR describes AEA’s “overall progress in implementing the study plan and schedule and the data collected, including an explanation of any variance from the study plan and schedule.” (18 CFR 5.15(c)(1)). Since filing the ISR in June 2014, AEA has continued to implement the FERC-approved plan for the Salmon Escapement Study. For example:  On September 30, 2014, AEA filed a Technical Memorandum with FERC to describe part of the methods, variances, and preliminary results of the 2014 Salmon Escapement Study (AEA 2014a). The methods and variances described therein were focused on activities conducted in the Middle and Upper rivers, and preliminary results were focused on Chinook Salmon (O. tshawytscha).  On October 15, 2014, AEA held an ISR meeting for the Salmon Escapement Study. In furtherance of the next round of ISR meetings and FERC’s SPD expected in 2016, this report contains a comprehensive discussion of results of the Salmon Escapement Study from the beginning of AEA’s study program in 2012, through the end of calendar year 2014. It describes the methods and results of the Salmon Escapement Study, and explains how all Study Objectives set forth in the Commission-approved Study Plan have been met. Accordingly, with this report, AEA has now completed all field work, data collection, data analysis, and reporting for this study. 2. STUDY OBJECTIVES The study objectives were established in RSP Section 9.7.1.2, and include: 1) Capture, radio-tag, and track adults of five species of Pacific salmon (i.e., Chinook, Chum (O. keta), Coho (O. kisutch), Pink (O. gorbuscha), and Sockeye (O. nerka) salmon) in the Middle and Upper Susitna River in proportion to their species-specific abundance. Capture and tag Chinook, Coho, and Pink Salmon in the Lower Susitna River. 2) Characterize the migration behavior and spawning locations of radio-tagged salmon in the Lower, Middle, and Upper Susitna River. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 2 October 2015 3) Characterize adult salmon migration behavior and timing within and above Devils Canyon. 4) If shown to be an effective sampling method, and where feasible, use sonar to aid in documenting salmon spawning locations in turbid water in 2013 and 2014. 5) Compare historical and current data on run timing, distribution, relative abundance, and specific locations of spawning and holding salmon. 6) Generate counts of adult Chinook Salmon spawning in the Susitna River and its tributaries to estimate the proportions of fish with tags for populations in the watershed. 7) Collect tissue samples to support the Fish Genetic Baseline Study (Study 9.14). 8) Estimate the system-wide Chinook Salmon escapement to the entire Susitna River, the Coho Salmon escapement to the Susitna River above the confluence with the Yentna River, and the distribution of Chinook, Coho, and Pink salmon among tributaries of the Susitna River (upstream of Yentna River confluence) in 2013 and 2014. 3. STUDY AREA As established by RSP Section 9.7.3, the study area encompassed the Susitna River from Cook Inlet upstream to the Oshetna River, or as far upstream as Chinook Salmon were detected (Figure 3-1), with an emphasis on wherever salmon spawned in mainstem habitats of the Susitna River. The mainstem Susitna River was divided into three segments: the Lower River (Project River Mile [PRM] 33–102.4), Middle River (PRM 102.4–187.1), and Upper River (PRM 187.1– 261.3). RSP Section 9.7.3 used Historical River Miles (RM) which were: Lower River (RM 30– 98), Middle River (RM 98–184), and Upper River (RM 184–260). Devils Canyon extends from approximately PRM 153.4 to PRM 166.1 (RM 150 to 163, respectively). Within Devils Canyon, the channel constricts and increases in vertical gradient to form three potential fish passage impediments (referred to as Impediments 1, 2, and 3) that may block or delay fish passage (see Section 3.2 in AEA 2013a for more detail on the impediments). 4. METHODS Descriptions of the study methods are organized below by objective. This was a multi-year study initiated in 2012 (AEA 2012, 2013a, 2014a,c). The description of the methods below is specific to the 2014 implementation of the study. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 3 October 2015 4.1. Objective 1: Capture, radio-tag, and track adults of five species of Pacific salmon in the Middle and Upper Susitna River in proportion to their abundance. Capture and tag Chinook, Coho, and Pink salmon in the Lower Susitna and Yentna rivers. In 2014, the study team implemented the methods with respect to Objective 1 as described in the Study Plan, with the exception of modifications described in Section 7.1.2 of the ISR and variances explained below (Section 4.1.6). Tasks to address Objective 1 were listed in RSP Section 9.7.4.1. 4.1.1. Fish Capture 4.1.1.1. Lower River In the Lower River, two fishwheels and gillnets were used to capture adult salmon for tagging in 2014. Capture sites were located approximately 1–2 river miles upstream of the Yentna River confluence. The fishwheels were operated from May 22 to August 26, at locations that were fished in 2010–2013 (Figure 3-1). One fishwheel operated on the west bank of the Lower River at PRM 33.4 for 1,153 hours, and the second fishwheel operated on the east bank at PRM 34.2 for 1,154 hours (Figure A-1). From May 22 to June 28, gillnets were fished in the vicinity of the fishwheels for a total of 79.2 hours (Figure A-2). The gillnet sampling was conducted to increase the probability of capture for larger Chinook Salmon, as it was expected that fishwheels would provide length-biased samples favoring smaller fish at this site (see ISR Part A Appendix I). The gillnets were 5.5 inch (in; stretch) or 7.5 in mesh, multi-strand web, 50–150 feet (ft) long, and 10–12 or 15–17 ft deep. Fifty-eight percent of the effort was with 7.5-in mesh and 42 percent with 5.5-in mesh. 4.1.1.2. Yentna River In addition, two fishwheels and gillnets were used on the lower Yentna River (RM 6) to capture adult salmon for tagging in 2014. Capture sites were in the same locations as had been operated for three decades (Figure 3-1). One fishwheel operated on the south bank of the Yentna River (RM 6) for 539 hours from May 22 to June 25, and the second fishwheel operated on the north bank of the Yentna River (RM 6) for 529 hours from May 22 to June 25 (Figure A-3). There was effort for both fishwheels every day in 2014 until June 26, and both fishwheels achieved the targeted effort (16 hours/day) on most days. From May 22 to June 25, gillnets (5.5 and 7.5-in mesh) were fished in the vicinity of the fishwheels for a total of 281.8 hours (effort was split 53 and 47 percent between mesh sizes, respectively; Figure A-4). The Study Plan provided that weirs would be used as a recapture method to support a two-event capture-recapture experiment to estimate abundance of Chinook Salmon in the Yentna River (RSP Section 9.7.4.8). In 2013, no suitable weir sites could be found. ISR Part C Section 7.1.2.6.1 indicated that the study team would modify the Study Plan and use fishwheels and gillnets for tag recapture in 2014. In 2014, a new site for two fishwheels and gillnetting was established on the Yentna River at RM 18 to recapture fish tagged at Yentna RM 6 (Figure 3-1). One fishwheel operated on the west bank of the Yentna River (RM 18.8) for 1,525 hours from May 24 to August 27, and the second fishwheel operated on the east bank of the Yentna River STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 4 October 2015 (RM 18.6) for 1,536 hours from May 24 to August 27 (Figure A-3). There was effort for both fishwheels on 96 days in 2014 until August 27, and both fishwheels achieved the targeted effort (16 hours/day) on 94 days. From May 24 to June 30, gillnets (5.5 and 7.5-in mesh) were fished in the vicinity of the fishwheels for a total of 230.8 hours (effort was split 46 and 54 percent between mesh sizes, respectively; Figure A-4). 4.1.1.3. Middle River In the Middle River, three fishwheels and gillnets were used to capture adult salmon for tagging in 2014. Two of the fishwheels were operated at the same two locations used in 1981–1985, 2012, and 2013 (sites 1 and 2), and a third fishwheel was operated at a site that was first used in 2013 (site 3; Figures 3-1 and 4.1-1). The addition of a third fishwheel represents a variance from the Study Plan (RSP Section 9.7.4.1.1): it was added to compensate for the lack of a Devils Canyon fishwheel (see ISR Section 7.1.2.1.2). The Middle River fishwheels consisted of two aluminum pontoons, three baskets, and two partially submerged live tanks for holding fish in river water. A tower and winch assembly were used to adjust the height of the baskets and ensure that the baskets were fishing within 20 centimeters (cm; 7.9 in) of the river bottom. Net leads were installed between the fishwheels and adjacent riverbank to direct fish away from the bank and into the path of the fishwheel baskets. During daylight operations, crews were never away from the fishwheels for more than one hour. From late June to mid-July, the fishwheels were left unattended overnight (from ~11:30 P.M. until 9:00 A.M. the following morning). From June 6 to September 7, the Site 1 fishwheel operated for 1,369 hours (61.3 percent of the time it was in place) on the west bank of the Susitna River (PRM 124.1; Figure A-5). Excluding the days it did not operate, daily fishing effort at Site 1 averaged 14.9 hours (range: 8.3–24 hours). The targeted amount of daily fishing effort at Site 1 varied by period: 13 hours from June 6–11, 15–17 hours from June 12–28, 24 hours from June 29 to July 18, 12 hours from July 19 to August 28, and 10 hours from August 29 to September 7. The Site 1 fishwheel did not operate during high water and heavy debris loads on June 26, June 27, and part of June 28. From June 12 to September 7, the Site 2 fishwheel operated for 1,270 hours (60.6 percent of the time it was in place) on the east bank of the river (PRM 123.0; Figure A-5). Daily fishing effort averaged 14.8 hours (range: 8.8–24.0 hours). Targeted daily fishing effort varied at Site 2: 15– 17 hours from June 12–29, 24 hours from June 30 to July 18, 12 hours from July 19 to August 28, and 10 hours from August 29 to September 7. The Site 2 fishwheel did not operate during high water and heavy debris loads on June 26, June 27, and part of June 28. From June 9 to September 7, the Site 3 fishwheel operated for 1,302 hours (60.2 percent of the time it was in place) on the west bank of the Susitna River at PRM 126.0 (Figure A-5). Daily fishing effort averaged 14.8 hours (range: 4.8–24.0 hours). Targeted daily fishing effort varied at Site 3: 15–17 hours from June 9–29, 24 hours from June 30 to July 18, 12 hours from July 19 to August 29, and 10 hours from August 30 to September 7. The Site 3 fishwheel was not operational during high water from June 26–28. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 5 October 2015 In 2014, the study team introduced a variance to the Study Plan and used set gillnets to capture Coho Salmon in September. RSP Section 9.7.4.1.1 stated that only fishwheels would be used to capture adult salmon for tagging in the Middle River. The FERC SPD recommended that the Middle River fishwheel operation extend throughout the month of September; however, as described in ISR Part C Section 7.1.2.1.2, analysis of sonar data from 2013 showed that the Middle River fishwheels were not effective in September when river discharge and turbidity decreased substantially from summer conditions. ISR Part C Section 7.1.2.1.2 indicated that the study team would modify the Study Plan and use a beach seine as an alternative capture method in September 2014. However, beach seine sites with suitable water depth and velocity, and in areas where fish were migrating (and not holding), were limited in the vicinity of Curry in 2014. The study team instead used set gillnets, which can be an effective capture method when a river is low and clear. In 2014, Middle River fishwheel operations ended on September 7, and every second day from September 10 to September 30 set gillnets were used to capture and tag adult Coho Salmon in the vicinity of Curry. Gillnets used in September were 18.3 m (60 ft) long and consisted of 7.6 cm (3.0 in) or 8.9 cm (3.5 in) multi-strand mesh. Fishing effort in September totaled 150.3 hours, and all sets were made between PRM 121.9 and PRM 126.0. On June 24, a set gillnet was used for 1.8 hours in the vicinity of Curry. This effort was simply exploratory in nature as crews investigated a few sites for the presence of Chinook Salmon. The gillnet used in June was 36.6 m (120 ft) long, 3.0 m (10 ft) deep, and had 8.9 cm (3.5 in) multi-strand mesh. The RSP (Section 9.7.4.1.1) indicated that at least one fishwheel would be operated in Devils Canyon below the impediments. However, as described in ISR Section 7.1.2.1.2, it was not feasible. 4.1.2. Radio-tagging Pulse-coded, extended-range tags by Advanced Telemetry Systems, Inc., (ATS; Isanti, MN, www.atstrack.com) were applied to a subset of salmon captured in the Lower River, Yentna River, and Middle River. There were 100 unique codes on each available frequency. Model F1835B transmitters (16 grams [g; 0.6 ounces (oz)], 30 cm [11.8 in] antenna, 96-day battery life) were used for small Chinook (mid-eye to fork [METF] length < 50 cm [19.7 in]) and Pink salmon; Model F1840B tags (22 g [0.8 oz], 30 cm [11.8 in] antenna, 127-day battery life) for Chum, Coho, and Sockeye salmon; and Model F1845B tags (26 g [0.9 oz], 41 cm [16.1 in] antenna, 162-day battery life) for large Chinook Salmon (METF ≥ 50 cm [19.7 in]). All transmitters were equipped with a mortality sensor that changed the signal pattern to an “inactive” mode for the remainder of the season once the tag became stationary for 24 hours. All of the radio tags were labeled with return contact information. Each tag was tested immediately prior to deployment to ensure it was functioning properly upon release. In the Lower River, only uninjured Chinook Salmon with a METF length of 50 cm (19.7 in) or greater (herein referred to as ‘large’), and Coho and Pink salmon with METF length of 40 cm (15.7 in) or greater, were radio-tagged. In the Yentna River, only large Chinook Salmon were radio-tagged. In the Middle River, large Chinook Salmon; Chinook Salmon measuring 30–49 cm METF (11.8–19.3 in; herein referred to as ‘small’); and Chum, Coho, Pink, and Sockeye salmon measuring 40 cm METF (15.7 in) or greater in length, were radio-tagged. Unless STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 6 October 2015 otherwise noted, all subsequent references to adult salmon sizes refer to METF lengths. No anesthesia was used in order to minimize handling time and tagging effects. Radio tags were inserted through the fish’s mouth into the stomach using a piece of PVC tubing (1/3-in diameter and 18 in long), with the tag antenna left to protrude from the mouth. All radio-tagged salmon were measured to determine METF length (to the nearest centimeter), and Chinook and Coho salmon were tissue sampled (axillary process) for genetic baselines (see Section 4.7). Unlike 2013, no salmon captured in the Middle River were sampled for scales (to age) or tagged with an external mark (i.e., spaghetti tag) in 2014. Eliminating these steps reduced the amount of handling time and the potential for any effects of handling fish on their post-release behavior. As stated in ISR Part A Section 4.1.8.3 and ISR Part C Section 7.1.2.1.2, scales would not be collected in 2014 in light of the fact that size selectivity would be tested (as in 2013) and that fish were randomly selected for tagging. RSP Section 9.7.4.1.3 indicated that a portion of fish captured in the Middle River (in excess of those required for radio-tagging) would be spaghetti- tagged to augment the ability to test assumptions about the representativeness of fish captured in the fishwheels. However, once the Indian River weir was rendered inoperable in 2014 (see Section 5.1.2.4 below for more details), it was recognized that there would be limited opportunity to sample fish on the spawning grounds, so no spaghetti tags were applied at the Middle River fishwheels. The inoperability of the weir also impacted the ability to assess size selectivity. Due to this variance, the study team relied on various other means to provide insights into capture probabilities based on size at the Middle River tag site. To minimize any effects of holding fish in live tanks, salmon captured during daylight hours were tagged upon capture. All fish were released immediately after tagging. All fish captured were inspected for tags. 4.1.3. Tagging Goals Recent (2012 and 2013) and historical (1981–1985) fishwheel catches, effectiveness, and salmon run timing guided tag application rates over the season. As stated in RSP Section 9.7.4.1, the goal for Chinook Salmon in the Lower River was to radio- tag 300 fish per fishwheel; numbers tagged were 259 salmon from the west bank fishwheel, and 271 from the east bank fishwheel (Table A-1; Figure A-6). The goal for gillnetting was 100 Chinook Salmon, and 129 salmon were actually radio-tagged. For Coho Salmon at the Lower River site, the goal was to radio-tag 300 fish per fishwheel; numbers tagged were 337 Coho Salmon from the west bank fishwheel, and 303 fish from the east bank fishwheel (Table A-1; Figure A-6). The difference between the goals and actual radio-tagging was the result of re- apportioning radio tags in season according to catches. The number of Pink Salmon tagged was similar to the tagging goal of 100 fish per fishwheel, with 106 radio-tagged from the west bank fishwheel, and 92 from the east bank fishwheel (Table A-1; Figure A-6). The radio-tagging goals for the Yentna River (RM 6) fishwheels were 100 fish per fishwheel; numbers tagged were 95 salmon from the north bank fishwheel, and 95 from the south bank fishwheel (Table A-2; Figure A-7). The goal for gillnetting was 100 Chinook Salmon; and 106 salmon were radio-tagged. Occasionally low catches in both fishwheels led to re-apportioning the radio tags in-season. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 7 October 2015 As stated in ISR Section 7.1.2.1.2, the revised goal for the Middle River was to radio-tag 650 Chinook Salmon (550 large and 100 small fish; from an initial goal of 400 large fish as stated in RSP Section 9.7.4.1). As described in the Study Plan, 200 each of Chum, Coho, Pink, and Sockeye salmon were targeted for radio-tagging in 2014. All species-specific goals were met or exceeded with the exception of small Chinook Salmon. Final 2014 radio-tagging numbers in the Middle River were 622 Chinook (590 large, 32 small), 200 Chum, 230 Coho, 201 Pink, and 200 Sockeye salmon (Table A-3; Figure A-8). 4.1.4. Numbers and Size of Marked and Unmarked Fish at Selected Locations Fish were randomly selected from the fishwheels for tagging. To assess whether these fish were representative of all fish in the river, several assumptions were tested for fish tagged at the Lower River, Yentna River, and Middle River sites, as described in RSP Sections 9.7.4.1.5, 9.7.4.1.7, and 9.7.4.6. 4.1.4.1. Lower River and Yentna River The assumption that radio-tagged salmon were representative of the population of salmon passing the tagging sites was evaluated for Chinook (Lower River and Yentna River) and Coho salmon (Lower River) in the framework of the mark-recapture experimental design. Heterogeneity in probability of capture was investigated by fish size (METF length), and spatially and temporally using mark-recapture diagnostic tests described by Seber (1982). These diagnostic tests, along with model selection procedures based on test results to minimize bias in estimates of abundance and distribution of spawners, are described more explicitly in Seber (1982, p. 438–439) and Arnason et al. (1996). The evaluations of the assumption of equal probability of capture and radio-tagging for all Chinook or Coho Salmon passing the Lower River tagging site were based on characteristics of all fish counted passing the Montana Creek and Deshka River weirs and of all marked fish “recaptured” at these weirs. Three independent tests were performed for data from each weir site to evaluate equal probability of capture by size and temporally, while spatial tests require d the data from both weirs. Similar tests for Chinook Salmon, by size and temporally (but not spatially), were conducted using fish sampled at the tagging and recapture sites on the Yentna River. No size-selectivity tests were conducted for Pink Salmon radio-tagged in the Lower River. 4.1.4.1.1. Temporal Equal-Proportions Test: Consistency of Chapman Model Contingency table analysis was used with the χ2 test for independence to conduct the temporal Equal-Proportions test (see Appendix I for details). For a weir site, the observations of numbers of fish passing the weir by day and number of recaptures passing the weir by day were divided into 2 to 6 pairs of cells by time period with approximately uniform sample sizes in each pair of cells. For each pair of cells, one contained the number of recaptured fish and the second contained the number of unmarked fish accounted for in the time period. This analysis determined if the ratio of marked-unmarked fish observed at each weir site was independent of time of capture at the weir. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 8 October 2015 4.1.4.1.2. Spatial Equal Proportions Test: Consistency of Chapman Model Contingency table analysis was used to conduct the spatial Equal-Proportions test (see Appendix I for details). A table (2 rows x 2 columns) containing the numbers of marked and unmarked fish observed at each of the two weir sites was used. This analysis determined if the marked-unmarked ratios were independent of weir site. 4.1.4.1.3. Equal Probability of Capture by Size Equal probability of capture by size was evaluated using the Kolmogorov-Smirnov (KS) two- sample test (see Appendix I for details). For the Lower River, this test determined if the distribution of lengths from all fish passing a weir site were similar to recaptured (radio-tagged) fish passing the weir site. Under the null hypothesis, the probability that a fish was radio-tagged was independent of the size of the fish, and the cumulative distribution function of the lengths of all fish passing a weir site were expected to be similar to the function of all recaptured fish passing that weir site. 4.1.4.2. Middle River RSP Section 9.7.4.1.5 stated that to test whether Chinook, Chum, and Sockeye salmon passing the Middle River fishwheels were equally vulnerable to being captured and radio-tagged, fish would be examined on selected spawning grounds to develop two primary metrics: estimates of the proportion of fish tagged (mark rate), and the size distributions of tagged and untagged fish. However, ground-based stream counts and carcass surveys conducted in the first study year (2012) did not produce adequate samples for generating these metrics (see Section 5.1.3.6 in AEA 2013a). As an alternative to stream counts and carcass surveys, the study team implemented a variance to the Study Plan in 2013 (ISR Section 4.1.8.3) and operated a picket weir on the lower Indian River approximately 1.6 river miles from the confluence with the Susitna River (Figure 3-1). The weir was designed to free-pass upstream- and downstream- moving adult fish past underwater video cameras. The weir was located far enough upstream to minimize the number of fish milling at the weir, yet far enough downstream to ensure that the majority of fish returning to the river would be available to count passing through the video chute. Given the success of the Indian River weir in 2013, the study team proposed that the Study Plan be modified to include similar methodology (see ISR Part A Section 4.1.5.2) in 2014, as described in ISR Part C Section 7.1.2.1.2. Unfortunately, the Indian River weir was rendered inoperable during a flood on June 26, 2014, prior to the onset of the majority of the salmon runs. For Chinook Salmon, the study team considered various alternatives for estimating mark rates and decided the best available option was to increase the number of aerial spawner surveys and aerial telemetry surveys in the Indian River (as described further in Section 4.6.1). Apart from the weir, there were no viable options available for collecting sufficient spawning ground samples to estimate the mark rates of Chum and Sockeye salmon, or to directly test for size selectivity in Chinook, Chum, and Sockeye salmon (the RSP did not call for the same effort on Coho and Pink salmon). For this reason the study team determined that applying spaghetti tags to fish in excess of radio-tagging requirements was not necessary. The inability to spaghetti tag fish in excess of radio-tagging requirements, or to collect spawning ground samples to estimate STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 9 October 2015 mark rates and assess size selectivity, was a variance to the Study Plan implemented in 2014. Results from previous study years and the analyses described in the following sections were used to provide insight with respect to these data gaps and ensure that all project objectives were addressed. As explained in ISR Section 4.1.8.3, external sexing of fish was not reliable at the Middle River fishwheels. Thus, fish sex was not recorded in 2014, a variance from the RSP (Section 9.7.4.1.7). 4.1.4.2.1. Fishwheel Effectiveness Across Time The main assumption of this study component was that the radio tags were deployed at the fishwheels in proportion to abundance for each species. To help evaluate this assumption at the Middle River fishwheels, the relative effectiveness of one Middle River fishwheel (at Site 1) was determined using sonar, from a ratio of the number of fish caught at the fishwheel to the number of fish observed using sonar. Sonar was not used at fishwheel sites 2 and 3 because these locations had steep banks and high river velocities that were not suitable for a fixed-site sonar. At Site 1, fish were observed with an Adaptive Resolution Imaging Sonar (ARIS) system operated in close proximity to the fishwheel across multiple time periods and river discharges. ARIS was also used to qualitatively assess fish approach behavior at the fishwheel relative to discharge and fish abundance. From June 3–25 and August 29 to September 30, one ARIS unit operated 24 hours per day on the right bank of the Susitna River immediately downstream of the Site 1 fishwheel (Figure 4.1-1). Daily review effort varied over the season, and ranged from a third (20 minutes per hour) of the imagery collected each day being reviewed (e.g., June 18–25, September 1–30) to all of the imagery collected each day being reviewed (e.g., June 3–17). The sonar sampling area ranged from 0.7–13.6 m (2.3–44.6 ft) in June and 0.7–14.0 m (2.3–45.9 ft) in August and September. The catch-per-unit-effort (CPUE; fish per fishwheel hour) for each fishwheel was compared over time and across a range of discharges to help evaluate the relative effectiveness of each fishwheel. For example, at Site 1, CPUE and ARIS data were used inseason to determine whether operational changes were required in order to increase fishwheel catch rates (e.g., high ARIS counts at a time of low CPUE suggested the fishwheel was not operating effectively and operational changes were required). 4.1.4.2.2. Differences Among Stocks To assess whether fish from a particular spawning area were right or left bank-oriented with respect to the capture site, the proportion of fish migrating into specific areas was compared with the collection bank. One concern was that mainstem fish could be more vulnerable to the fishwheels because they linger or mill upstream and downstream of capture sites. Recaptures of radio-tagged fish at the tagging site fishwheels provided a good test of whether milling fish were exposed to greater capture rates. In addition to quantitative and qualitative assessment of subsequent behavior of these recaptured fish, the final destinations (mainstem/tributary) of recaptured fish were compared with other tagged fish to determine whether fish that spawned in the mainstem were recaptured at a higher rate. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 10 October 2015 4.1.4.2.3. Equal Probability of Capture by Size Since no salmon were sampled for lengths on the spawning grounds above the Middle River tag site in 2014, size selectivity at the Middle River fishwheels could not be directly tested. Some comparisons were made using KS tests to provide insight into the potential for size-selective sampling. For each species, cumulative length-frequency distributions were compared for: 1) fish captured at each of the Middle River fishwheels; and 2) fish captured at the Middle River fishwheels and those that were radio-tagged. For the first set of comparisons, the null hypothesis was that fish of different length were captured with equal probability, regardless of capture location. A significant test result would indicate that the fishwheels did not capture fish of different length equally. For the second set of comparisons, the null hypothesis was that fish had an equal probability of being selected for tagging from those captured, regardless of length. A significant test result would indicate that fish of different length were not selected equally for tagging. 4.1.5. Examining Handling-Induced Changes in Behavior As stated in RSP Section 9.7.4.1.6, an assumption of this study was that the behavior of radio- tagged fish was not affected by the capture and handling process or by the presence of a radio tag thereafter. The study design allowed for some fish dropping back and not resuming their upstream migration, and these radio-tagged fish were removed from the experiment and subsequent analyses. For the remaining fish, it was assumed that capture and tagging did not affect final spawning destinations or migration behaviors, once they had recovered from the tagging event and resumed migration. Four analyses were proposed in the RSP that would provide insight into the validity of that assumption. First, comparisons of travel times between recently-tagged and distantly-tagged fish could reveal handling effects if the recently-tagged fish moved more slowly through a common river reach. Second, performance comparisons could reveal handling effects if fish that were subjected to higher fishwheel holding densities (or longer holding times) performed worse than fish with lower-density (or shorter-duration) holding experiences. Third, examination of mark rates at spawning locations could provide an indication of possible handling-induced changes in behavior. Finally, comparisons of post-release migratory behavior between once-handled (fish that were never recaptured) and multiple-handled (fish that were recaptured in the fishwheels post-release) could provide data on the effects of the fish capture process, including any potential cumulative handling effects. In 2014, handling-induced changes in behavior were evaluated using the first and fourth proposed methods. In 2014, holding times and densities were always low, thus it was not possible, nor necessary, to test whether longer times or higher densities impacted fish behavior or survival (i.e., it was not possible to perform the second proposed test). Also, since the Indian River weir was rendered inoperable in 2014, it was not feasible to make comparisons of mark rates, hence the third proposed test could not be performed. For Middle River fishwheels, potential handling effects were evaluated based on the post-release migratory behavior of recovered vs. recently-handled fish (first proposed test), and of once-handled vs. multiple- handled fish (fourth proposed test). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 11 October 2015 For the Yentna River mark-recapture experiment (Table B-5), the number of dart-tagged Chinook Salmon was deprecated based on an estimate of the proportion of radio-tagged fish not migrating upstream. 4.1.6. Variances RSP Section 9.7.4.1.1 stated that fishwheels would be used to capture adult salmon for tagging in the Middle River. ISR Part C Section 7.1.2.1.2 indicated that the study team would modify the Study Plan to add beach seining as an alternative method for sampling in September. Instead, set gillnets were used to capture Coho Salmon in September 2014. As described in Section 4.1.1.3, beach seine sites with suitable water depth and velocity, and in areas where fish were migrating (and not holding), were limited in the vicinity of Curry in 2014. Gillnets proved to be an effective alternative capture method. Relative to using fishwheels, this variance increased the study team’s ability to achieve Objective 1. As an alternative to stream counts and carcass surveys to obtain mark rates and length samples, the study team implemented a variance to the Study Plan in 2013 (ISR Part A Section 4.1.8.3) and operated a picket weir on the lower Indian River approximately 1.6 river miles from the confluence with the Susitna River. This variance was carried over and conducted in 2014. RSP Section 9.7.4.1.3 indicated that a portion of Chinook, Chum, and Sockeye salmon captured in the Middle River (in excess of those required for radio-tagging) would be spaghetti-tagged to augment the ability to test assumptions about the representativeness of fish captured in the fishwheels. As described in Section 4.1.4.2, once the Indian River weir was rendered inoperable in 2014, the study team recognized that there would be limited opportunity to sample fish on the spawning grounds, so no spaghetti tags were applied at the Middle River fishwheels. This was a variance to the Study Plan that reduced the likelihood of being able to test whether fish were equally vulnerable to being captured and tagged over time. In lieu of this variance, the study team used fixed-site sonar at Site 1, bank of capture comparisons, and various length-frequency comparisons to provide insights into capture probabilities at the Middle River tag site (as described in Section 4.1.4.2). In addition to the variances described above, the study team also implemented several modifications to the Study Plan methods in 2014, as proposed in ISR Section 7.1.2. RSP Section 9.7.4.1 indicated that 700 Chinook Salmon would be radio-tagged in the Yentna River in 2014. As proposed in ISR Section 7.1.2.1.1, the study team modified the Study Plan such that all Chinook Salmon captured at the Yentna River tag site in 2014 were marked with uniquely numbered dart tags, of which up to 300 fish were also to receive radio tags. This modification to the tagging strategy in the Yentna River better supported the study team’s objective (Objective 8) to estimate Chinook Salmon escapement to the entire Susitna River (see Section 4.8.1 for more detail). RSP Section 9.7.4.1.1 indicated that at least one fishwheel would be operated in Devils Canyon below the impediments from late June through late July to explore whether the sample size of radio-tagged Chinook Salmon moving into and above the impediments could be increased. However, as described in ISR Section 7.1.2.1.2, it was not feasible to operate a fishwheel in Devils Canyon for a variety of reasons (e.g., uncertainty with respect to catch rates at an STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 12 October 2015 unproven site, logistical challenges, and cost). No fishwheels were operated in Devils Canyon in 2013 or 2014. The study team implemented two additional variances to the Study Plan in 2014 to compensate for the lack of a Devils Canyon fishwheel: three fishwheels were operated in the vicinity of Curry (RSP Section 9.7.4.1.1 indicated two fishwheels would be used), and the number of radio tags allocated to Chinook Salmon was increased to 650 (RSP Section 9.7.4.1 indicated that 400 radio tags would be used). These variances were first implemented in 2013 (ISR Section 4.1.8.1; the radio tag goal for Chinook Salmon in 2013 was increased from 400 to 560). All three of these variances were proposed modifications to the Study Plan described in ISR Part C Section 7.1.2.1.2 that increased AEA’s ability to achieve Objective 1. In 2014, the study team implemented a variance to the Study Plan to provide for a total of 650 Chinook Salmon to be radio-tagged in the Middle River in 2014, with a goal of tagging 550 large (METF ≥ 50 cm [19.7 in]) and up to 100 small (METF < 50 cm [19.7 in]) Chinook Salmon. RSP Section 9.7.4.1.2 stated that 400 large Chinook Salmon would be radio-tagged in the Middle River. In 2013, modifications to increase the tag goal (from 400 to 560 large Chinook Salmon) and radio tag small Chinook Salmon were both implemented as variances (ISR Sections 4.1.8.1 and 4.1.8.2). As indicated in RSP Section 4.1.8.2, small Chinook Salmon comprised a substantial portion of the catch in 2013, so it was deemed worthwhile to apply some tags to this segment of the population to help characterize spawning locations. The study team proposed this change as a modification to the Study Plan in ISR Part C Section 7.1.2.1.2. In both years, this variance increased AEA’s ability to achieve Objectives 1, 2, and 3. RSP Section 9.7.4.1.6 indicated that AEA would assess the effects of holding time and density on the behavior of tagged fish. Due to stipulations in the Fish Resource Permit, all fish were tagged soon after capture in 2014, thereby reducing holding times and densities to levels that made comparisons of post-release survival and migration behavior unnecessary. This variance was implemented in 2013 (ISR Section 4.1.8.3) and 2014 and it was proposed as a Study Plan modification in ISR Part C Section 7.1.2.1.2. This variance did not affect achieving study Objective 1. RSP Section 9.7.4.1.7 indicated that contingency table analyses would be used to compare the sex and age composition of radio-tagged fish. However, as stated in ISR Section 4.1.8.3, early in the 2013 field season it became clear that correctly identifying fish sex based on external morphological characteristics would be difficult at the Middle River fishwheels. Fish sex was therefore not recorded in 2014. Scales were also not collected from salmon in 2014, in light of the fact that size selectivity would be tested, fish were randomly selected for tagging, and the study team minimized the amount of handling time for each fish. Contingency table analyses were not conducted in 2014, which was a Study Plan variance that was proposed as a modification in ISR Part C Section 7.1.2.1.2. The same variance was implemented in 2013 (ISR Section 4.1.8.3). Since size selectivity could not be directly tested in 2014 due to the Indian River weir being rendered inoperable, this variance decreased the ability to assess capture probabilities at the Middle River fishwheels (Objective 1). To meet this Objective without recapture data, the study team relied on length-frequency comparisons and results from previous study years to provide insights into capture probabilities based on size at the Middle River tag site. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 13 October 2015 4.2. Objective 2: Determine the migration behavior and spawning locations of radio-tagged fish in the Lower, Middle, and Upper Susitna River In 2014, AEA implemented the methods with respect to Objective 2 as described in the Study Plan, with the exception of modifications described in Section 7.1.2 of the ISR and variances described below (Section 4.2.4). Tasks to address Objective 2 were listed in RSP Section 9.7.4.2. Three groups of radio-tagged fish were tracked: 1) adult Chinook, Coho, Chum, Pink, and Sockeye salmon radio-tagged at the Middle River fishwheels (PRM 123–126); 2) Chinook, Coho, and Pink salmon radio-tagged in the Lower River (PRM 33–34); and 3) Chinook Salmon radio-tagged in the lower Yentna River (Figure 3-1). The three study components and data analyses were tightly coordinated. All mobile and fixed-station receiver data were analyzed together, and analysis products were characterized in a consistent manner. The primary function of the telemetry component was to track these tagged fish spatially and temporally with a combination of fixed and mobile receivers. Time/date stamped, coded radio signals from tags implanted in fish were recorded by fixed-station or mobile positioning. All telemetry gear (tags and receivers) was manufactured by ATS. The types of behavior characterized included the following:  Arrival and departure timing at specific locations/positions  Direction of travel  Residence time at specific locations/positions  Travel time between locations/positions  Identification of migratory, holding, and spawning time and locations/positions  Movement patterns in and between habitats in relation to water conditions (e.g., discharge, temperature, and turbidity). These data, in conjunction with habitat descriptions, allowed characterization of migratory behavior and final destinations for salmon in mainstem habitats (main channel, slough, side channel, and tributary mouths) and tributaries. Spawning or final locations of tagged fish were used to determine the number and proportion of the tagged fish of each species using mainstem habitats. 4.2.1. Fixed-station Monitoring Stand-alone operating telemetry arrays were deployed at strategic locations in the Lower, Middle, and Upper River to provide migration checkpoints, develop spawning ground inventories, and monitor the destinations of individual tagged fish. In total, 20 fixed-station receiver sites were operated in 2014 (Figure 3-1; Table C-1). This was one more fixed-station STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 14 October 2015 monitoring site from what was proposed in the RSP Section 9.7.4.2.1 and three less sites than were implemented in 2013 (ISR Part A, Section 4.2.1). The receiver site proposed for the Indian River weir was not operated in 2014 since the weir was rendered inoperable by a flood. Ten fixed-station receiver sites were operated in the Lower River in 2013 and 2014 and were largely chosen to represent significant tributaries that were known to contain Chinook Salmon. Eight sites were located in tributaries (Lower Yentna, Skwentna, Upper Yentna, Talkeetna, Chulitna, Deshka Weir, Montana Creek Weir, and Middle Fork Chulitna Weir) and two sites were located on the mainstem Susitna River (Deshka Mouth [PRM 40] and Sunshine [PRM 83]). All Lower River sites were equipped with two antennas to detect both upstream and downstream passage (Table C-1). In 2014, the first Lower River site began operating the week of May 19 and all sites were demobilized by the week of September 22 (Table C-2). Middle and Upper River sites were chosen to both provide geographic separation of the Middle River area to describe migration and spawning behaviors, and monitor at the appropriate resolution to quantify passage through Devils Canyon and the Upper River. Five fixed-station receiver sites were operated in the Middle River within or below Devils Canyon, all of which were located on the mainstem Susitna River (Lane Creek area [PRM 116.7], Middle River Gateway [PRM 130.1], Indian River confluence [PRM 142.0], Cheechako Creek [PRM 157.3], and Chinook Creek [PRM 160.4]). One fixed-station receiver site was operated on the mainstem Susitna River in the Middle River above Devils Canyon (Devils Island [PRM 166.9]). All Middle River sites were equipped with at least two antennas to detect upstream and downstream passage (Table C-1). One antenna at the Indian River site was oriented directly up the tributary to detect fish moving into the lower Indian River. In 2014, the first Middle River site began operating the week of April 28 and all sites were demobilized by the week of October 27 (Table C-3). Four fixed-station receiver sites were operated in the Upper River, all of which were located in the mainstem Susitna River (Watana Dam site [PRM 187], Watana Creek [PRM 197], Kosina Creek [PRM 209], and Oshetna River [PRM235]). Each of these sites was equipped with two antennas to detect upstream and downstream passage. The Watana Creek, Kosina Creek, and Oshetna River sites were also equipped with a third antenna to detect fish passage into the respective tributaries (Table C-1). The first Upper River site began operating the week of April 28 and all sites were demobilized by the week of October 13 (Table C-3). All receivers scanned Chinook Salmon tag frequencies to begin the season. Additional frequencies were scanned as other species were captured and tagged in the fishwheels. Fixed-station receivers were manually downloaded at least once a week. All fixed stations were designed to cover the entire channel width of the respective tributary or mainstem Susitna River. The Lane Creek and Watana Dam sites were operated with two receivers (all other sites had just one receiver). Lane Creek operated with one receiver scanning the tag frequencies deployed in the Lower River and the other receiver scanning the tag frequencies deployed in the Middle River (Table C-1). The Watana Dam site fixed-station operated with one receiver scanning all salmon tag frequencies and a second receiver, operating as a backup, scanning all Chinook Salmon frequencies. Additional methods pertaining to the set-up and operation of fixed-station receiver sites were provided in RSP Section 9.7.4.2.1. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 15 October 2015 4.2.2. Aerial Telemetry Surveys In 2014, aerial telemetry surveys were conducted in the Lower River by fixed-wing aircraft and helicopter, and in the Middle and Upper River by helicopter. Survey coverage included the mainstem Susitna River from the mouth (PRM 0) upstream to areas above the Clearwater Creek confluence (PRM 266.6) in the Upper River, as well as most major tributaries. Surveys targeting adult salmon began on June 11 and continued through October 30 (Table C-4). Surveys were scheduled to cover each section of the river (Lower, Middle, and Upper rivers) at least once every five days for helicopter surveys and every 14 days for fixed-wing surveys. Survey timing was adjusted depending on the observed fishwheel catches in the Lower and Middle rivers. Helicopter surveys were conducted at lower elevations (100–200 m [328–656 ft]) and at slower speeds than possible with fixed-wing aircraft, and therefore allowed more time for signal acquisition, higher spatial resolution, and fish habitat observations. In general, the spatial resolution of helicopter surveys was approximately 300 m (984 ft). Higher precision was achievable in reaches where conditions were most favorable and observers could determine whether the fish was in off-channel or mainstem habitat. Fixed-wing aerial telemetry surveys provided fish locations to the nearest river mile and helped to characterize the destinations of radio-tagged fish. Although the fixed-wing surveys provided less precise spatial resolution of fish locations (and habitat use) than the helicopter surveys, they more effectively covered the large lineal distances of the Susitna River tributaries where higher spatial resolution was not required. The mainstem aerial telemetry surveys covered over 265 river miles, and multiples of that total when side channels and braids of the Lower River were included. To allocate survey effort efficiently and to the highest priority needs, resolution was a function of fish behavior. The highest priority and highest resolution needs were for fish that appeared to be holding or spawning. For migrating fish, resolution to the nearest 500 m (1,640 ft) of river was generally sufficient. Frequent surveys enabled high-resolution and time-intensive tracking effort to identify the exact locations of spawning and holding fish. During salmon spawning periods, the crew used a GPS with a Geographic Information System (GIS) based map containing the locations of each fish during the previous survey. Locations where fish were repeatedly observed were further investigated to ensure an accurate position for the fish and look for visual evidence of spawning activity. Geographic coordinates were recorded for each signal detected using an integrated communication link between the telemetry receiver and a GPS unit. The position of the fish was determined by the position of the aircraft at the time of the highest signal power. Range testing of the mobile aerial setup was conducted in the Middle River to confirm detection ranges for typical flying heights, and receiver gains, as well as to work with the helicopter pilot to refine the methods for achieving highest spatial resolution. Tag identification and GPS coordinates were archived and systematically processed after each survey. A data-handling script was used to extract unique tag records with the highest power level from the receiver files generated during the survey. These records were imported into a custom database software application (Telemetry Manager) and incorporated into a GIS-based mapping database. Geographically and temporally stratified data for radio-tagged fish were STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 16 October 2015 provided to the habitat sampling team (Study 9.9) and Instream Flow Study (Study 8.5) to inform their field sampling efforts. 4.2.2.1. Lower River Surveys Aerial telemetry surveys of the Lower River and tributaries covered areas from the mouth of the Susitna River to the confluence of the Chulitna River (PRM 102.4). From June 12 to October 30, 63 fixed-wing and helicopter surveys were conducted in the Lower River (Table C-4). Fixed-wing telemetry surveys were conducted in the Lower River and tributaries from June 24 to September 23. Tag frequencies for Chinook Salmon (tags released in the Lower and Middle rivers) were scanned during Lower River fixed-wing surveys from June 24 to August 11, after which there was little value in collecting additional detections from the inactive tags. Tag frequencies for Coho Salmon were scanned through September 23 (except for August 8 and August 11 in the Yentna River when no tag frequencies for Middle River Coho Salmon were scanned). Tag frequencies for Chum, Pink, and Sockeye salmon released in the Middle River, as well as Pink Salmon released in the Lower River, were not scanned during fixed-wing surveys in the Lower River. These fish were tracked during helicopter surveys (Table C-4). Helicopter telemetry surveys were conducted in the Lower River and tributaries from June 12 to October 30. The mainstem Susitna River was highly braided in this section with side channels and sloughs, so complete coverage with the helicopter required approximately one day of effort. Lower River tributaries were typically surveyed for approximately three miles. More extensive surveys were conducted in tributaries during the fall to locate pink salmon tags in tributaries. Tag frequencies for Chinook Salmon were scanned during Lower River helicopter surveys from June 12 to August 31. Tag frequencies were scanned through September 30 for Chum and Pink salmon, October 16 for Sockeye Salmon, and October 30 for Coho Salmon. 4.2.2.2. Middle River Surveys Helicopter telemetry surveys of the Middle River and tributaries were conducted from June 11 to October 28 and covered mainstem areas from the confluence of the Chulitna River (PRM 102.4) through Devils Canyon to the proposed Watana Dam site (PRM 187.1). The reach from the Chulitna to Portage Creek required approximately one day to complete and was conducted on 63 days (Table C-4). The river between Devils Island (PRM 166.9) and the proposed Watana Dam site was usually flown on the same day as the surveys of the Upper River, but also on additional days to download fixed stations (a total of 49 days). Once adult salmon were observed entering Devils Canyon, the Susitna River and its tributaries from Portage Creek to Devils Island were surveyed every 1–2 days (June 28 to August 6) to ensure information on passage timing, hold duration, and potential spawning destinations. These frequent flights continued until upstream movement of fish in Devils Canyon had stopped. During the peak Chum and Sockeye salmon migration and spawning periods in the Middle River, the river section from the mouth of the Chulitna River to Devils Island was surveyed every third day (August 9 to October 28) to monitor the movements of salmon into spawning and holding locations. The interval between flights, shorter than the proposed 5-day interval (RSP Section 9.7.4.2.2), was proposed as a Study Plan modification that was detailed in ISR Part C Section 7.1.2.2 and implemented in 2014. For both Middle and Upper River helicopter surveys, tag frequencies were scanned STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 17 October 2015 through August 31 for Chinook Salmon, September 30 for Chum and Pink salmon, October 28 for Sockeye Salmon, and October 30 for Coho Salmon. The increased frequency of aerial telemetry surveys conducted on the Middle River in 2014, from Whiskers to Portage creeks, provided multiple detections for most of the radio-tagged fish in this portion of the river. These detections before, during, and after the spawning period allowed for the identification of fish that were likely migrating and/or spawning in mainstem habitats. In 2014, there was a high degree of consistency between the potential spawning sites identified for radio-tagged Sockeye Salmon, and to a lesser degree Chum Salmon, and the visually confirmed redd sites for these species. This consistency provided further support as to the usefulness of using radio telemetry to detect spawning locations in mainstem habitats of the Susitna River. 4.2.2.3. Upper River Surveys Helicopter telemetry surveys of the Upper River and tributaries were conducted from June 11 to October 14 and covered the mainstem areas from the proposed Watana Dam site (PRM 187.1) to upstream of the Clearwater Creek confluence (PRM 266.6). This reach included approximately 48 relatively confined river miles. This survey required approximately one day to complete and was conducted on 23 days (Table C-4). It took less time to complete this survey when done in conjunction with Middle River surveys because less conveyance time was required. Helicopter telemetry surveys of the Upper River generally were triggered by the detection of fish moving above the Devil Creek fixed station. When tags were detected above Devils Canyon, the aerial telemetry survey continued upstream and into the tributaries until all of the tag(s) that had passed were located. Surveys for Chinook Salmon were conducted until the end of August. 4.2.3. Telemetry Data Analysis 4.2.3.1. Data Filtering As in 2012 and 2013, the following criteria were set for tag detection records to be considered valid in 2014: 1) for fixed-station receiver data, there must have been at least five detections recorded on directional antennas per minute (single records, or records separated by more than one minute were rejected; all detections on the pooled master antennas were ignored; the criterion at noisy receivers was 7 signals in one minute (slight noise) or 100 hits in 60 minutes (severe noise); 2) for mobile data, single detections were allowed but were closely scrutinized on a case-by- case basis; and 3) any detections requiring unrealistic travel times were removed. Additional details regarding telemetry data processing were provided in Section 4.2.5.4 of the 2012 study report (AEA 2013a). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 18 October 2015 4.2.3.2. Classifying Spawning Destinations and Holding Locations The detection history of each individual radio-tagged salmon was examined in order to classify the fish to one of two types of “spawning/holding destination” (Tributary vs. Mainstem Destination) based on temporal patterns in their detection positions. Spawning and holding locations for salmon are considered together from a biological and analytical perspective for the study. The study team used detection histories, instead of final tag locations, to assign a destination because salmon can drift downstream after spawning or death, obscuring the true destination and distribution of the fish. The algorithms that were used to define potential fish spawning behavior were developed based on assumptions about fish behavior and physiology. The specific steps used to classify each fish are described below. Since these classifications were based on telemetry detections, they cannot be considered as ‘confirmation of spawning’, hence terms such as ‘likely’ and ‘possible’ were used in this report (see below). Confirmation of spawning/holding required further research, including the use of sonar for Chinook Salmon in turbid water (Objective 4), as well as aerial, boat-based, or visual surveys for all salmon species. As much as possible, the analyses described below were performed in season, and provided a list of potential locations for field crews to visit for confirmation of spawning activity. Not all fish could be assigned to one of the two types of spawning/holding destination. For example, several fish were excluded from analyses because they exhibited unexpected behaviors that suggested potential handling-related effects. The detections of these fish indicated that: 1) the fish never left the release area, 2) the tag was never detected, or 3) the fish moved only in a downstream direction. Other tagged fish had tracking histories that suggested they never arrived at their spawning destination and thus, these fish were assigned to a classification called “Other Mainstem.” The Other Mainstem category comprised all fish that were included in the analyses but which could not be assigned to one of the two destination categories. Fish assigned to the Tributary Destination category included all salmon that moved into a tributary river or stream flowing into the Susitna River, presumably for spawning, regardless of whether they subsequently returned to the mainstem Susitna River. The tributary into which the fish entered was recorded as its spawning “stock.” Any fish that entered one tributary, exited, and subsequently entered another tributary was given a stock assignment based on the tributary where more time was spent. Any fish that spent less than 6 days in a tributary was examined as described below, in order to assess it as a possible mainstem spawner. The study was not designed to identify exact spawning positions within the tributaries, so spawning locations in tributaries were not described. After classifying the Tributary Destination fish, all remaining fish were classified on a fish-by- fish basis either into the Mainstem Destination or Other Mainstem category. Classifications were assigned after displaying a fish's complete detection history over ortho-rectified aerial imagery, and examining each detection in succession. Before proceeding with classification, several detections were eliminated from consideration. First, movements were ignored after the tag entered mortality mode (unless the movements indicated the tag’s sensor malfunctioned). Second, downstream movements following the most upstream location were typically ignored as the fish may have already been dead or moribund, unless the fish showed subsequent upstream STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 19 October 2015 movements. Third, all fish that died within two weeks of tagging were classified immediately into the Other Mainstem category. The remaining data were examined, looking for any geographically aggregated cluster of detections which might indicate that the fish should potentially be included in the Mainstem Destination category. Clusters in which the last live detection was earlier than the published species-specific spawn-timing window (Schmidt and Bingham 1983, Barrett et al. 1985a,b, Jennings 1985, Thompson et al. 1986) were ignored as they were considered to be aberrant data points possibly resulting from bear-kills, angler-catches, handling related-mortalities, or other causes of mortality, and they were unlikely to have been at their spawning destination at the time of death (n = 32 combined for 2012, 2013, 2014). Each remaining cluster was scrutinized before placing it into one of two ‘relative spawning probability’ groups:  Clusters were classified as “likely” spawning/holding locations based on the number of repeat detections and density of unique detections in the same area. A likely spawning cluster consisted of multiple unique detections in relatively close proximity to one another. The number of unique detections was variable, but could be as few as three if they were grouped tightly enough together; the distance between unique detections also varied, but was generally within a few hundred meters (< 1,000 ft). In these cases, the fish was assigned to the Mainstem Destination category.  Clusters were classified as “possible” spawning/holding locations if the cluster was made up of fewer unique detections (sometimes only two); or, when more unique detections were present, but the locations were loosely aggregated. Also included in this group were clusters located in areas where fish may have been holding (rather than spawning, e.g., tributary mouths). These fish were also assigned to the Mainstem Destination category.  When tags were physically recovered in a known mainstem spawning area (based on data for the 1980s studies), the recovery location was listed as a “likely” Mainstem Destination, regardless of the nature of the detection cluster. Salmon in the Mainstem Destination category had a cluster of detections in the potential spawning/holding area, and a single representative location was selected from within the cluster. The single selected point was either the last live detection, the detection with the most powerful signal strength, or a detection that appeared to represent the center of mass of the cluster. For each fish, the position of its potential spawning/holding location was plotted on ortho-rectified aerial imagery in order to categorize the habitat as either Side Channel/Slough, Tributary Mouth, or Mainstem Proper, based on the position of the potential spawning location. All tag locations classified as spawning locations (“likely” and “possible”) following radio-telemetry analysis were further investigated to confirm spawning and holding activity. This included the use of sonar for Chinook Salmon in turbid water, as well as aerial, boat-based, and visual surveys for all salmon species. The timing of spawning events could not be confirmed using radio-telemetry. However, the tracking data were used to estimate approximate spawn-timing windows. For each fish assigned to Mainstem Destinations, their single representative location had an associated date. Taken together, the dates corresponding to all the representative locations had a distribution, whose 10th and 90th percentiles were used to describe mainstem spawn timing. For Tributary Destinations, the dates when fish were detected within the assigned spawning tributary were used to generate a STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 20 October 2015 spawn timing distribution. Detections in mortality mode were excluded, and so were fish that went to minor tributaries (i.e., those for which less than 10 percent of the fish in that river section had destinations). Tributary spawn timing was described in terms of the 10th and 90th percentiles of the dates in this distribution. 4.2.4. Variances RSP Section 9.7.4.2.1 listed ten fixed-station receiver sites to be used in the Middle and Upper rivers. ISR Part C Section 7.1.2.2 described a Study Plan modification to include eleven fixed- station receiver sites in the Middle and Upper rivers in 2014, of which seven sites were listed in RSP Section 9.7.4.2.1 (Lane Creek, Gateway, Indian River confluence, Cheechako Creek, Chinook Creek, Devil Creek area, and Kosina Creek) and four sites were not (Indian River weir, Watana Dam sonar site, Watana Creek, and Oshetna River). However, only ten sites were used in 2014 (see Section 4.2.1). One of the eleven sites proposed for 2014 was planned for the Indian River weir in order to detect radio-tagged salmon passing the weir. Since the weir was rendered inoperable by a flood on June 26 prior to the start of the Chinook Salmon migration into the Indian River, this fixed-station receiver site was not operated in 2014. This variance did not affect AEA’s ability to achieve Objective 3. RSP Section 9.7.4.2.2 indicated that aerial telemetry surveys would be scheduled at five-day intervals with the intent to ensure a maximum of seven days between surveys with weather contingencies. In 2014, the study team implemented a variance to the Study Plan and increased the frequency of surveys in the Middle River to every 1-2 days between Portage Creek and Devils Island from June 28 to August 6, and every third day between the Chulitna River and Devils Island from August 9 to October 28 (as described in Section 4.2.2.2). This change was a proposed Study Plan modification described in ISR Part C Section 7.1.2.2. This variance improved the resolution of the geographic positions of tagged fish in the Middle River (below, within, and above Devils Canyon) and helped AEA achieve study Objectives 1, 2, and 3. 4.3. Objective 3: Characterize adult salmon migration behavior and timing within and above Devils Canyon In 2014, AEA implemented the methods with respect to Objective 3 as described in the Study Plan, with the exception of modifications described in ISR Part C Section 7.1.2 and variances described below (Section 4.3.5). Tasks to address Objective 3 were listed in RSP Section 9.7.4.3. 4.3.1. Fixed-station Monitoring A combination of aerial telemetry surveys and fixed stations below, within, and above Devils Canyon was used to determine the migration timing and behavior of radio-tagged salmon that passed into the Upper River (Figure 3-1). As described in Section 4.2.1, four fixed-station receiver sites were deployed in the Upper River (Watana Dam site, Watana Creek, Kosina Creek, and Oshetna River) at locations where they had the highest probability of detecting radio-tagged salmon. Fixed stations deployed at the confluences with Kosina Creek and the Oshetna River were used to guide aerial survey efforts needed to identify spawning areas in the Upper River. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 21 October 2015 4.3.2. Aerial Telemetry Surveys Aerial telemetry surveys were conducted via helicopter within and above Devils Canyon from June 11 to October 28. These surveys provided location data for radio-tagged fish in areas that were not directly monitored by fixed-station receiver sites (i.e., in the mainstem between fixed- station receiver sites, and within tributaries). These detections assisted with the successful tracking of fish movements within and above Devils Canyon, providing day-to-day locations, passage timing, and hold durations. Surveys were typically flown until all radio-tagged fish known to have passed the Devil Creek fixed station had been detected. Additional details on the range of survey dates, survey frequency, and survey coverage were provided in Section 4.2.2, and details regarding the 2014 Study Plan variance to increase the frequency of aerial telemetry surveys were provided in Section 4.2.4. Aerial telemetry data were critical for the identification of potential spawning behavior and detecting potential spawning and holding locations. The goal of 300 m (984 ft) accuracy of geographic position when locating tagged fish, including spawning and holding fish (RSP Section 9.7.4.2.2), was achieved by the combined effect of airspeed, flight path, antenna direction, and receiver gain control. In addition, the aerial detections contributed to the estimation of detection efficiencies for each fixed station. The timing and proportion of all tagged salmon that passed Devils Canyon was calculated and compared with the remaining tagged population, and their final spawning locations were identified. The total number of radio- tagged fish that were detected at or above Gateway Station (PRM 130.1) was used to estimate the proportion of fish that migrated through the Middle River and above Devils Canyon. 4.3.3. Aerial Spawner Surveys Aerial spawner surveys (by visual observation via low-flying helicopter) to determine the distribution and relative abundance of adult Chinook Salmon were conducted in Susitna River tributaries within and above Devils Canyon, upstream to and including the Oshetna River. These surveys helped to identify potential spawning locations for Chinook Salmon that may not be represented by the radio-tagged segment of the population. A total of seven aerial spawner survey events were conducted at approximate weekly intervals from July 14 through August 19, 2014 (Table 4.3-1). The survey extent covered the same major tributaries and clear-water areas of the Susitna River as during 2012 and 2013 (Table 4.3-1; Figure 4.3-1). From Cheechako Creek to the Oshetna River, a total of 18 streams were surveyed; 12 tributaries to the Susitna River and six secondary tributaries. All streams were surveyed from their confluence up to 3,000 ft in elevation, to a predetermined barrier to anadromous fish passage, or to the stream’s headwater origin, whichever came first. Additionally, two lakes in the Tsisi Creek drainage were surveyed in 2014 specifically to look for spawning Sockeye Salmon. Based on anecdotal information from a pilot that flies planes frequently in the Upper River, Alaska Department of Fish and Game (ADF&G) staff requested AEA extend the spawner surveys to include these locations and evaluate the claim that sockeye salmon may to be spawning in these lakes. Five surveys of the Tsisi lakes were conducted from late July to mid-August 2014 (Table 4.3-1). Survey confidence was estimated independently for each stream during each survey event by ranking three variables that may have affected the observers’ ability to see fish: 1) sun glare on the water; 2) clarity of the water (i.e., turbidity, not white water created by rapids); and 3) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 22 October 2015 overhanging vegetation. Variables were ranked from zero to four, where four indicated optimal survey conditions and zero indicated poor survey conditions. Quality-control measures included employing two observers on all surveys, with one observer remaining consistent throughout the study. Observers communicated fish sightings to each other and when necessary, the flight was slowed or halted until both observers had confirmed the number of fish present. The helicopter pilot was consistent for survey events two through five. Observer efficiency was evaluated with a one-time paired independent aerial spawner survey during the peak of Chinook Salmon spawning in Indian River. 4.3.4. Using Sonar to Enumerate Salmon at the Proposed Dam Site As stated in Section 1 of the FERC SPD, it was recommended that AEA evaluate the feasibility of putting in a weir or operating a sonar counting station at or near the dam site in the next year of study to count fish migrating through Devils Canyon (FERC 2013a). Prior to the 2013 field season, operation of a weir near the dam site was determined to be not feasible due to the physical limitations of a weir withstanding the normal range of discharges for the mainstem Susitna River. In 2013, AEA assessed the feasibility of placing a sonar counting station at or near the dam site (see ISR Appendix G). Results from 2013 field activities showed that it was likely feasible to count salmon-sized fish and corroborate counts with radio-telemetry. In 2014, AEA used sonar (ARIS) to count the number of salmon-sized fish passing the proposed Watana Dam site (PRM 187.1) from July 6 to August 22. Methods describing this study component were provided in the September 2014 Implementation and Preliminary Results Technical Memorandum (AEA 2014a) and Appendix G. The primary objectives for this study component were to estimate the number of net upstream-moving Chinook Salmon that passed through the sonar beams, and to describe temporal (daily and hourly) and spatial (range of passage) patterns of Chinook Salmon observations. AEA also implemented a variance to the Study Plan in 2014 and collected bathymetry and water-velocity profiles (acoustic Doppler current profiler, ADCP) at the monitoring sites to corroborate assumptions regarding the fish migration corridor at this site. 4.3.5. Variances RSP Section 9.7.4.2.2 indicated that aerial telemetry surveys would be scheduled at five -day intervals. As proposed in ISR Section 7.1.2.2, AEA modified the Study Plan by increasing the frequency of aerial telemetry surveys from the mouth of the Chulitna River to Devils Island to three-day intervals in 2014. Increasing the frequency of aerial telemetry surveys was also implemented as a variance in 2013 (ISR Section 4.3.5). This modification enhanced AEA’s ability to characterize migration behavior and achieve study Objective 3. To support further assessment of the fish migration corridor at the Watana Dam sonar site, AEA conducted seven ADCP transects in 2014. To supplement the velocity transects, bathymetric data were also collected. These two tasks were variances to the Study Plan that increased the likelihood of AEA achieving study Objective 3. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 23 October 2015 4.4. Objective 4: Use available technology to document salmon spawning locations in turbid water In 2014, AEA implemented the methods with respect to Objective 4 as described in the Study Plan, with the exception of modifications described in Section 7 of the ISR and variances described below (Section 4.4.3). In late July 2014, AEA used a Dual Frequency Identification Sonar (DIDSON) to characterize suspected Chinook Salmon spawning in turbid water of mainstem habitat of the Middle River. This was a variance to the Study Plan since RSP Section 9.7.4.4 indicated that sonar would be used to characterize any suspected salmon spawning in turbid water. As described in ISR Part A Section 6.5, sonar was not able to differentiate between Chum, Coho, Pink, and Sockeye salmon due to overlap in their run timing and body size. This variance was proposed as a Study Plan modification in ISR Part C Section 7.1.2.4. The use of a DIDSON in 2014 followed the RSP (Section 9.7.4.4), but varied from what was used in 2013 (ARIS; see ISR Part C Section 7.1.2.4), yet both tools have similar capabilities. Potential Chinook Salmon spawning locations were identified from historic spawning locations reported in the 1980s, and from 2012, 2013, and 2014 detections of radio-tagged fish. Sites with physical features indicative of Chinook Salmon spawning habitat were also sampled on an opportunistic basis. Potential spawning sites that could not be accessed by boat, or sites with physical characteristics not suitable for sonar sampling (e.g., low water or entrained air), were not sampled. Additional site-specific information was collected and subsequently relayed to the Fish and Aquatics Instream Flow Study (Study 8.5) team. 4.4.1. Sonar Equipment and Methods The sonar system consisted of a Sound Metrics Corporation (SMC) DIDSON, SMCX2 dual-axis rotator assembly, data transmission cable, top-side control box, laptop computer with DIDSON data acquisition software (version 5.26.06), and portable external hard drive. The system was deployed using a pole-mounted winch that was secured to the gunwale of a jet-drive boat. Once the sonar was lowered to an appropriate depth (which varied depending on site-specific conditions), the SMCX2 rotator was used to pan and tilt the sonar from the surface to search for fish activity. The system was powered by a battery bank consisting of four, 12 -volt (V), deep- cycle batteries. The battery bank was recharged at the end of each day with a 2,000 watt (W) Honda EU2000i generator. At each site, the boat was moored and the DIDSON was lowered to scan for suitable substrate and/or the presence of Chinook Salmon. If suitable substrate was observed, a scan was performed until fish were observed, or for a maximum of 15 minutes (if no fi sh were observed). Once fish were observed, recording of sonar imagery was initiated. If suitable substrate was not present or no fish were observed at the sample site, the DIDSON was retrieved and the system was relocated to another site. Only habitats with features known to support salmonid spawning (as defined by Groot and Margolis 1991; Quinn 2005) were surveyed. Sites were excluded if:  The location was in the thalweg of the mainstem Susitna River, with no structure providing relief from the river flow. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 24 October 2015  The location was an area of high velocity with no holding areas (i.e., greater than 1.5 meters per second [m/s; 4.9 feet per second (ft/s)]).  The location consisted of shallow water with high velocity.  The location was in the middle of a rapid or area with high velocity.  The location had unsuitable substrate (i.e., mud, silt).  Excessive amounts of entrained air occurred at the sample location. Data were initially collected using 10–20 m (33–66 ft) sample windows. Typically the sonar unit was tilted down to allow the sample beams to spread along the substrate throughout as much of the sample range as possible. In reaches with a non-uniform slope or that had obstructions present, the DIDSON depth and tilt angle were adjusted as necessary to maximize c overage of the substrate. When fish were located and spawning activity was suspected, up to 30 minutes of data were collected. Adjustments of the pan and tilt angles were made as required to maintain visual observation of individual fish. Due to the presence of Chum Salmon at some sites, the minimum total length (TL) for a target to be considered a Chinook Salmon was increased to 80 cm TL (31.4 in). Data were collected using a frame rate of eight frames per second. Data were ported directly to external hard drives, and backed up and archived to additional hard drives after each survey. Locational data were collected using a hand-held GPS unit to allow for geo-referencing of sample locations. 4.4.2. Sonar Data Analysis and Reporting Data processing involved playing back the streaming data files using DIDSON software. Files were reviewed to note the following for each survey:  Presence or absence of Chinook Salmon. When adult salmon-sized fish were detected, total lengths of individual fish were estimated using the software’s sizing tool.  Presence or absence of spawning behavior activities. Behavior of individual fish was reviewed and observations of spawning activities (redd digging or covering, redd guarding, paired fish, aggressive territorial behaviors, egg laying, milt expulsion, quivering) were noted.  Presence or absence of redds. 4.4.3. Variances RSP Section 9.7.4.4 indicated that sonar would be used to characterize any suspected salmon spawning in turbid water of the mainstem habitats of the Susitna River (as indicated by radio- telemetry analysis). As discussed in Study 9.7 ISR Part A Section 6.5 for 2013, the use of sonar was limited particularly in shallow water, close to the shoreline, and at sites where multiple species of similar body lengths were present. It was confirmed that many of the shallow STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 25 October 2015 locations that were not accessible for sonar use were spawning sites for Chum and Sockeye salmon. Thus in 2014, the study team implemented a variance to the Study Plan and used sonar only to characterize suspected Chinook Salmon spawning. This variance was proposed as a Study Plan modification in ISR Part C Section 7.1.2.4. Although this variance limited the ability to document spawning locations in turbid water for Chum, Coho, Pink, and Sockeye salmon in 2014, the Objective was met by demonstrating that sonar is not an effective tool given the typical habitat conditions where these species spawn. 4.5. Objective 5: Compare historical and current data on run timing, distribution, relative abundance, and specific locations of spawning and holding salmon AEA implemented the methods with respect to Objective 5 as described in the Study Plan with no variances. Comparisons were made between this study’s results (2012–2014) and historical results (1981–1985) that characterized the relative abundance; locations of spawning and holding salmon; and use of mainstem, side channel, slough, and tributary habitat types by adult salmon. Research conducted in the early 1980s provided annual abundance estimates (1983–1985) relevant to at least four fishwheel sites along the Susitna River. These abundance estimates were apportioned to mainstem, sloughs, and tributaries. The 1980s studies relied heavily on visual observations of fish and abandoned late-season redds, and therefore, may have underestimated the use and relative importance of mainstem habitats, many of which occur in turbid water during a substantial portion of the spawning period. Another concern was that data collected approximately 30 years ago may not characterize the current habitat use by salmon in the mainstem Susitna River. This study addressed both of these concerns by deploying a similarly scaled study of the spawning runs to the Susitna River in 2012, 2013, and 2014, and by using radio telemetry and sonar technology not available in the 1980s. Both methods provided a more rigorous characterization of the use of mainstem habitats than methods used in the 1980s. To the extent spawning distribution and habitat use in the current study were similar to earlier studies, the current study greatly increased the sample size and confidence in the conclusions from studies in both periods. 4.5.1. Variances No variances from the methods described in the Study Plan occurred during the 2014 study season. 4.6. Objective 6: Generate counts of adult Chinook Salmon spawning in the Susitna River and its tributaries The study team implemented the methods with respect to Objective 6 as described in the Study Plan, with the exception of modifications described in Section 7.1.2 of the ISR, and variances described below (Section 4.6.1). In 2014, this objective was addressed by attempting to operate a weir on the Indian River (see Section 4.1.4.2) and conducting aerial spawner surveys in the Indian River (see Section 4.3.3). The purpose of this work was to establish survey-area mark STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 26 October 2015 rates (proportion of fish tagged in different areas) that would support inferences about the representativeness of tagging across spawning stocks. In addition, mark rates from these areas could be used to estimate the abundance of salmon passing the tagging sites (the weir was rendered inoperable by a flood; the ensuing study variances are described in detail in Section 4.6.1). The aerial spawner surveys were not intended to provide a direct estimate of the total Chinook Salmon abundance. Instead, they provided a minimum count, and then helped to establish minimum and likely tributary-specific mark rates, as was done for Portage Creek (2012) and the Indian River (2012 and 2013) in previous years. Concurrent aerial telemetry surveys were conducted in the Indian River in July and August 2014 to determine the number of live radio-tagged Chinook Salmon present. Protocols developed based on 2012 and 2013 experiences were implemented in 2014 to survey the Indian River. Multiple aerial telemetry surveys were flown bracketing the entire spawning period of Chinook Salmon. Survey aircraft were equipped with telemetry receivers and GPS to identify positions of radio-tagged fish. 4.6.1. Variances RSP Section 9.7.4.6 indicated that Chinook Salmon would be examined on selected spawning grounds to test whether fish were equally vulnerable to being captured and radio-tagged. Results from the 2012 Adult Salmon Distribution and Habitat Utilization Study filed in February 2013 (AEA 2013a) indicated that it would be unlikely to obtain sufficient numbers of fish samples through spawning ground surveys to provide a robust mark rate, and in-turn, an estimate of the numbers of fish above Devils Canyon (as established during the FERC Study Dispute process). Therefore, prior to the 2013 field season, the study team decided to replace the spawning ground surveys with the operation of a weir and underwater video system, along with a fixed-station receiver site, on the Indian River to enumerate tagged and untagged fish, and establish mark rates. This change was implemented as a variance in 2013 (ISR Section 4.1.8.3) and 2014, and was a proposed modification to the Study Plan described in ISR Part C Section 7.1.2.1.2. The methods and approach of using weirs to obtain this information was consistent with RSP Sections 9.7.4.1.5 and 9.7.4.6. The same two metrics (i.e., mark rate and size distribution of tagged/untagged fish) would be developed from fish counts at the weir (and telemetry detections) that would have been developed from spawning ground surveys. However, as described in Sections 4.1.4.2 and 5.1.2.4, the Indian River weir was rendered inoperable by a flood on June 26, 2014, prior to the onset of the Chinook Salmon run. In response, the study team considered alternative methods for estimating the mark rate of Chinook Salmon in the Indian River to ensure the study objective was met. One option considered was to install a sonar unit at or near the weir site to count passing fish. However, the advantages of being able to install a sonar unit soon after the weir was rendered inoperable were outweighed by the fact that multiple salmon species would be present in the river by mid-July, and thus Chinook Salmon could not be reliably counted (since sonar cannot distinguish between species). The study team considered conducting a gillnet operation below the Indian River weir site to capture and sample Chinook Salmon. Although physically handling fish is a reliable method of collecting mark-rate and length data, physical conditions in the lower river were not suitable for gillnetting, so it was unlikely crews could capture a sufficient number of fish. Also, there may be negative impacts on fish health due to the capture and handling process. The study STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 27 October 2015 team also considered stream walks to count Chinook Salmon, but their experiences in 2012 proved this method was unlikely to succeed. The timing of the loss of the weir was shortly before the onset of the Chinook Salmon run into the Indian River. Thus, an immediate adjustment was required to ensure data were collected that would fulfill the objectives of estimating the escapement of Chinook Salmon returning to the Indian River and establishing a mark rate that could be used to make inferences about the relative abundance among recovery locations (e.g., above the proposed dam site). AEA decided that the best available option to fulfill the objectives was to increase the number of aerial spawner surveys and aerial telemetry surveys (every third day during the spawning period) in the Indian River. Instead of using the underwater video system at the weir, fish counts from aerial spawner surveys and area-under-the-curve (AUC) methods (Ames and Phinney 1977; English et al. 1992) were used to generate an escapement estimate for Chinook Salmon returning to the Indian River in 2014. The marked fraction of Chinook Salmon present in the Indian River, as well as estimates of residence time (i.e., the length of time Chinook Salmon were present in the river) that were required for the AUC approach were estimated from aerial detections of radio-tagged fish. Additional information related to AUC methods is provided in Appendix H. No fish length-frequency information was collected using these methods. 4.7. Objective 7: Collect tissue samples to support the Fish Genetics Study AEA implemented the methods with respect to Objective 7 as described in the Study Plan with no variances. The task for this study component was to collect genetic samples from adult anadromous salmon in conjunction with addressing Objectives 1 and 2. Tissue samples were taken from all radio-tagged salmon. Sample collections were coordinated with the Genetic Baseline Study team (see ISR Study 9.14). 4.7.1. Variances No variances from the methods described in the Study Plan occurred during the 2014 study season. 4.8. Objective 8: Estimate the system-wide Chinook Salmon escapement and the Coho Salmon escapement to the Susitna River above the Yentna River, and the distribution of those fish among tributaries of the Susitna River In 2014, AEA implemented the methods with respect to Objective 8 as described in the Study Plan, with the exception of modifications described in Section 7.1.2 of the ISR, and variances described below (Section 4.8.1). A commonly applied two-event, capture-recapture experiment was used to estimate the annual abundance of Chinook Salmon in the entire Susitna River drainage, and the Coho Salmon abundance in the Susitna River above the Yentna River confluence. Due to the river channel configuration and availability of acceptable fishing sites, the abundance of Chinook Salmon for the entire Susitna River was designed to be the sum of two independent experiments: the abundance of Chinook Salmon in the Lower River (Susitna River STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 28 October 2015 above the Yentna confluence) plus the abundance of Chinook Salmon in the Yentna River. At the Lower River site, the capture event was provided by fishwheels and (and gillnets for Chinook Salmon only) operated throughout the seasonal salmon migration. Substantial effort was made to apply radio tags in proportion to abundance. Later in the salmon migration, recaptures were collected from tributary weir and sonar sites forming the second event of the experiment. In the Yentna River, the capture event was also provided by fishwheels (and gillnets for Chinook Salmon only) operated throughout the seasonal salmon migration, with effort expended to apply radio tags and also dart tags to fish in proportion to abundance. Radio tags were applied according to average run timing, and dart tags were applied to every healthy Chinook Salmon measuring 50 cm (19.7 in) METF or greater. The recaptures were collected from fishwheels (and gillnets for Chinook Salmon only) located 19.3 kilometers (km; 12 miles [mi]) farther upstream on the Yentna River. The abundance at the fishwheel sites on the Susitna (Chinook and Coho salmon) and Yentna (Chinook Salmon) rivers was estimated. Length and timing information from the tagged and untagged fish was used to assess the validity of most assumptions. Behavior of radio-tagged fish following tagging also provided information for evaluating two critical assumptions: knowing how many tagged fish have “entered” the experiment, and whether their behavior compromised the experiment. Two fishwheels and drift gillnets were operated on the Lower River from May 22 to August 26, 2014, to capture fish for marking with radio tags (Table A-1; Figures A-1, A-2, and A-6). Two fishwheels and drift gillnets were operated on the Yentna River (RM 6) from May 22 to June 25 to capture fish for marking with radio tags and dart tags (Table A-2; Figures A-3, A-4, and A-7). Lengths of tagged and untagged fish, and a tissue sample from radio-tagged fish (for genetics sampling), were collected at each site. Dart tags (model FT-1-94 from Floy® Tag, Seattle, WA) were 15 cm (6 in) long, yellow, and uniquely numbered, with the unique number printed twice on each tag. Fish at Yentna RM 6 were tagged on the left side, immediately below the dorsal fin, with the barb on the tag inserted deep enough to lock into the dorsal pterygiophores. A hole- punch was used to make a hole in the adipose fin as a secondary mark to detect tag loss. Weirs on tributaries of the Lower River were used to recapture tagged fish and estimate the proportion of each species’ run that had a tag. At the weir recapture sites, Chinook Salmon were counted visually and tagged fish were detected by a fixed-station receiver adjacent to the weir (Table C-2). A weir was operated on the Deshka River from May 19 to September 2, 2014, (Table B-1) and on Montana Creek from June 4 to September 21, 2014 (Table B-2). The use of Montana Creek as a weir site represented a variance from the proposed Willow Creek site, as described in the ISR (Part A, Section 4.8.1). Fish length was sampled at each site for estimating the number of Chinook Salmon (METF ≥ 50 cm [19.7 in]) and testing assumptions of the mark- recapture experiment. Sonar was operated on the Middle Fork Chulitna River from June 20 to August 3, 2014 (the use of sonar rather than a weir represents a variance from the RSP, as described in the ISR, Part A, Section 4.8.1). At the sonar site, the total number of fish passing was counted by examining the recorded sonar files post-season, and tagged fish were detected by a fixed-station receiver adjacent to each sonar site (Table C-2). In the Yentna River drainage, two fishwheels and gillnet operations were conducted at RM 18 to recapture tagged Chinook Salmon from May 24 to August 27, 2014 (Figure A-3). In 2013 and 2014, this represented a variance from the RSP (Section 9.7.4.8), as detailed in the ISR (Part A, Section 4.8.1). All Chinook Salmon were examined for a dart tag and an adipose fin punch. If a STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 29 October 2015 tag was found, the distal half of the tag, containing one of the unique tag numbers, was clipped off and retained as an absolute record of the tag found. Fish length was also sampled at this site for testing assumptions of the mark-recapture experiment. A two-event, capture-recapture experiment was also used to estimate the abundance of Coho Salmon in the Lower River. Two fishwheels were used on the Lower River (PRM 33–34) to capture Coho Salmon for marking with radio tags, from July 6 to August 26, 2014 (Table A-1; Figures A-1, A-2, and A-6). Coho Salmon were counted, inspected for tags, and a sample measured for length at the weirs on the Deshka River and Montana Creek, as described above. 4.8.1. Variances RSP Section 9.7.4.8 indicated that weirs would be operated on the Middle Fork Chulitna River and Willow Creek among other locations to inspect fish for estimating the proportion with tags. However, Montana Creek was selected as a weir site instead of Willow Creek in 2014. Montana Creek had a more uniform channel configuration and lower water velocity than Willow Creek. The two creeks were located near each other, had similar discharge and watershed characteristics, and had similar Chinook and Coho salmon run sizes. This variance also occurred in 2013 (ISR Part A Section 4.8.1) and did not affect AEA’s ability to achieve study Objective 8. A weir was not operated for Chinook Salmon on the Middle Fork Chulitna River in 2013 (see Study 9.7 ISR, Part A, Section 4.8.1) or 2014. In June of both years, the stream discharge was too high for weir installation; instead, the sonar unit designated for Lake Creek was reassigned to the Middle Fork Chulitna River in order to obtain the counts necessary for the abundanc e experiment. River discharges remained too high to install the weir at a later date. While a sonar was operated in 2014, post-season data analysis revealed that focus and aiming problems prevented obtaining reliable counts and length measurements. This variance did not prevent meeting the abundance and distribution objectives for the Lower River and the Susitna River abundance estimate component of study Objective 8 in either year but required additional assumptions for generating the Chinook Salmon escapement estimate. Without the Middle Fork Chulitna River, it was assumed that all Chinook Salmon stocks in the Susitna River were adequately represented by the Deshka River and Montana Creek stocks for the tag recapture sampling. Depending upon how similar the Middle Fork Chulitna River sampling results had been to the Deshka River and Montana Creek results, the accuracy and precision of the Lower River Chinook Salmon abundance estimate could be affected in either a positive or negative way. RSP Section 9.7.4.8 indicated that weirs would be operated on the Talachulitna River and Lake Creek in the Yentna River drainage. No weir operations occurred at either location in 2014. Results from the 2013 (see ISR Section 4.8.1 for a description of the 2013 variance) and 2014 studies showed that stream discharges in both systems remained too high for weir installation. As proposed in ISR Section 7.1.2.6.1, AEA modified the Study Plan and used two fishwheels and gillnets in the Yentna River (RM 18) as recapture methods in 2014 instead of weirs. This modification helped AEA achieve study Objective 8 in 2014. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 30 October 2015 5. RESULTS This section summarizes results from 2012 and 2013 and presents detailed results from the 2014 salmon escapement study. Detailed results from the 2012 and 2013 studies were summarized elsewhere (AEA 2013a, 2014a,c; Yanusz et al. 2013). Data developed in support of this document are available for download at http://gis.suhydro.org/ Post_ISR/09-Fish_and_Aquatics/9.7-Salmon_Escapement/ and include the files:  9_7_ESCAPE_20141219_QC3_LGL_CurrySonarData_2014.xlsx;  9_7_ESCAPE_20141219_QC3_LGL_FishwheelData_2014.xlsx;  9_7_ESCAPE_20141219_QC3_LGL_GillnetData_2014.xlsx;  9_7_ESCAPE_20141219_QC3_LGL_Susitna Salmon Flat Tables 2014.xlsx;  9_7_ESCAPE_20141219_QC3_LGL_TagRecoveryData_2014.xlsx;  9_7_ESCAPE_20141219_QC3_LGL_TelemMgr Salmon Database 2014.zip;  9_7_ESCAPE_20141219_QC3_LGL_WatanaSonarData_2014.xlsx;  9_7_ESCAPE_20150106_QC3_LGL_Turbid Water Sonar_2014.xlsx;  SuWa LGL FAQ Escapement 201407 Indian River Weir Fish Passage Database QC3 KM 20140808.xlsx. 5.1. Objective 1: Capture, radio-tag, and track adults of five species of Pacific Salmon in the Middle and Upper Susitna River in proportion to their abundance. Capture and tag Chinook, Coho, and Pink salmon in the Lower Susitna and Yentna rivers. A total of 9,286 adult salmon of five species were radio-tagged during this three-year study. Table 5.1-1 presents the number of tags implanted and tagged fish tracked for each species in each year of the study. While the tagged species were consistent each year in the Middle River, they varied each year in the Lower River (Table 5.1-1) in order to assist ADF&G with fulfilling statewide objectives regarding escapement to the Susitna River Basin. The size of fish radio-tagged ranged from 28 cm (11.0 in) to 110 cm (43.3 in.) METF. Since fishwheels were the primary capture method and have the potential for biased catch based on size, several analyses for size-selective capture and tagging were conducted. When size- selective capture was detected adjustments were made as detailed below. During each study year, tracking began with the first tags implanted in the Lower River and continued through October or November. Detailed results related to fish capture, tagging, and tracking in 2014 are presented below and in Appendices A, B, and C. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 31 October 2015 5.1.1. Fish Capture and Fish Tagging 5.1.1.1. Lower River In the Lower River, 2,048 Chinook Salmon (1,471 large, 577 small) were caught and 659 large Chinook Salmon were radio-tagged (Table A-1; Figure A-6, Figure A-9). The peak of Chinook Salmon catch in the fishwheels occurred on May 29 (111 fish) and CPUE for an individual fishwheel peaked at 5.6 Chinook Salmon per hour (west bank). Daily radio-tag deployment in the Lower River peaked at 39 Chinook Salmon on June 5. Seventy-seven percent (129) of large Chinook Salmon captured using gillnets in the Lower River were radio-tagged. Large Chinook Salmon captured in the Lower River averaged 67.6 cm [26.6 in] METF and small Chinook Salmon averaged 39.1 cm [15.4 in] METF (Table A-4). A total of 1,513 Coho Salmon were captured in the Lower River in 2014, of which 640 were radio-tagged at the fishwheels. The peak catch occurred on August 3 (128 fish) and CPUE for an individual fishwheel peaked at 7.0 fish per hour (west bank). The daily number of radio tags deployed peaked on July 28 (71 tags). Pink Salmon were the most abundant species captured in the Lower River (13,934 fish). Daily fishwheel catches peaked on July 20 (2,050 fish) and CPUE peaked at 130 fish per hour (west bank). A total of 198 Pink Salmon were radio-tagged, and the most tags deployed on a single day was 16 (July 24). Similar to Coho Salmon, Pink Salmon catches were consistently higher in the west bank fishwheel relative to the east bank fishwheel. A total of 6,577 Chum and 853 Sockeye salmon were also captured in the Lower River in 2014. 5.1.1.2. Yentna River Of the 3,025 Chinook Salmon (1,357 large, 1,668 small) captured at Yentna RM 6, the majority (87 percent) were captured in fishwheels and the remainder (13 percent) in gillnets (Table A-2). Daily catch peaked at 275 fish on June 4. The magnitude and timing of the peak CPUE for Chinook Salmon was very similar for the north and south bank fishwheels (Figure A-10). The average length of large Chinook Salmon captured at Yentna RM 6 (66.9 cm [26.3 in] was similar to that of fish captured in the Lower River, while small Chinook Salmon at Yentna RM 6 averaged 34.9 cm (13.7 in) METF, which was 4.3 cm (1.7 in) less than at the Lower River. A total of 190 large Chinook Salmon were radio-tagged at the Yentna RM 6 fishwheels (Table A-2; Figure A-7), which was 7.3 percent of the total fishwheel catch. In the gillnetting at Yentna RM 6, 106 large Chinook Salmon were radio-tagged, which was 27 percent of the gillnet catch. Radio-tag deployment for Chinook Salmon at Yentna RM 6 peaked at 24 tags on June 2. Of the 2,305 adult Chinook Salmon (1,375 large, 930 small) captured at Yentna RM 18, the majority (95 percent) were captured in fishwheels, and the remaining 5 percent in gillnets (Table A-2). Daily catch peaked at 171 fish on June 5. The magnitude and timing of the peak CPUE for Chinook Salmon was very similar for the north and south bank fishwheels (Figure A-10). At Yentna RM 18, large and small Chinook Salmon averaged 66.8 cm (22.3 in) and 37.0 cm (14.6 in) METF, respectively (Table A-4). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 32 October 2015 5.1.1.3. Middle River In the Middle River, 877 Chinook Salmon (672 large, 205 small) were captured in 2014 of which 876 were captured in fishwheels and one was captured in a gillnet (Table A-5,Table A-57). The daily catch of large Chinook Salmon peaked at 58 fish on July 1 (Figure A-11), and CPUE was highest for large Chinook on June 30 (1.6 fish/hour at Site 1; Figure A-12). The mean length of large Chinook Salmon captured in the Middle River (71.6 cm [28.2 in]; Table A-6) was greater than the mean length of large Chinook Salmon captured in the Lower River (67.6 cm [26.6 in]) or Yentna RM 6 (66.9 cm [26.3 in]) fishwheels (Table A-4). Radio tags were applied to 590 large and 32 small Chinook Salmon captured at the fishwheels (Table A-3; Figure A-8), which represented 88 and 16 percent of the fish captured, respectively. Daily tag deployment peaked at 51 for large Chinook Salmon and 3 for small Chinook Salmon. In total, 1,552 Chum Salmon were captured in the Middle River of which 1,469 were captured in fishwheels and 83 in gillnets (Table A-5). Daily catch for Chum Salmon peaked at 89 fish on August 11 and CPUE peaked at 3.7 fish/hour on August 10 (Site 3; Figure A-12). Chum Salmon caught in the Middle River averaged 57.9 cm (22.8 in) METF (Table A-6). Radio tags were applied to 200 Chum Salmon at the fishwheels, or 14 percent of the fishwheel catch (Table A-3; Figure A-8). Daily tag deployment peaked at 12 for Chum Salmon. A total of 377 Coho Salmon (375 large, 2 small) were captured in the Middle River of which 198 were caught in fishwheels and 42 in gillnets (Table A-5). Daily catch peaked on August 22 (30 fish) and CPUE peaked at 1.4 fish/hour at Site 3 on July 29 (Figure A-12). Coho Salmon captured in the Middle River averaged 54 cm (22.8 in; Table A-6). A total of 230 Coho Salmon (daily max = 13 tags) were radio-tagged in the Middle River in 2014 of which 212 were captured at fishwheels and 18 in set gillnets (Table A-3; Figure A-8). In total, 7,473 Pink Salmon were caught in the Middle River fishwheels in 2014 (Table A-5). Peak daily catch was 979 fish (July 29) and peak daily CPUE was 52.0 fish/hour at Site 3 (July 29; Figure A-12). The mean length of Pink Salmon captured in the Middle River was 46 cm (18.1 in) METF (Table A-6). A total of 201 Pink Salmon were radio-tagged (2.7 percent of all fish caught), with a peak daily allocation of 15 tags (Table A-3; Figure A-8). Two-hundred and thirty four Sockeye Salmon (223 large, 11 small) were caught in 2014, of which 223 were caught at fishwheels and 11 in gillnets (Table A-5). Peak catch occurred on July 28 (13 fish) and peak CPUE occurred on July 22 and August 7 at Site 2 (0.7 fish/hour; Figure A- 12). The mean length of Sockeye Salmon was 49 cm (19.3 in) METF (Table A-6). Radio tags were applied to 200 Sockeye Salmon (95 percent of total catch), with a peak of 13 tags deployed on a given day (Table A-3; Figure A-8). The largest proportion of Chum Salmon were captured at Site 1 (39.6 percent), whereas the largest proportion of Sockeye Salmon were captured at Site 2 (42.2 percent), and the largest proportion of Chinook (48.2 percent), Coho (59.1 percent), and Pink (58.7 percent) salmon were captured at Site 3. In general, radio tags were deployed in proportion to catch for Chinook and Sockeye salmon, but less so for Pink, Chum, and Coho salmon (Figure A-11). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 33 October 2015 Seven other fish species were captured and released at the Middle River fishwheels, including 68 Rainbow Trout O. mykiss, 67 Round Whitefish Prosopium cylindraceum, 17 Arctic Grayling Thymallus arcticus, 13 Humpback Whitefish Coregonus pidschian, 13 Longnose Sucker Catostomus catostomus, 8 Dolly Varden Salvelinus malma, and 2 Burbot Lota lota (Table A-6). During Middle River gillnet operations, 1 small Chinook, 83 Chum, 42 Coho, and 11 Sockeye salmon (including one fish measuring less than 40 cm METF) were captured (Table A-5). Other species captured during gillnetting included Rainbow Trout, Round Whitefish, Arctic Grayling, Longnose Sucker, and Bering Cisco Coregonus laurettae. 5.1.1.4. Programmatic Summary Catch rates in the Lower River, Yentna River, and Middle River were sufficient to achieve all of the requirements of study Objective 1, and met or exceeded the majority of species-specific tag goals in each of the three study years. There were only seven cases where less than 90 percent of the allocated radio tags were deployed. These cases included large Chinook Salmon in the Lower River in 2012 and 2013 (88 and 83 percent of tags were deployed, respectively), and in the Yentna River in 2013 (61 percent of tags were deployed). Catches of large Chinook Salmon at both of these locations were substantially greater than the tag goals, and not meeting the tag targets in these cases was an artifact of investigators attempting to deploy tags proportional to abundance over the entire run rather than due to low catch rates. The remaining three cases occurred in the Middle River and included large Chinook Salmon in 2012 (88 percent of tags deployed), small Chinook Salmon in 2014 (32 percent of tags deployed), and Sockeye Salmon in 2012 and 2013 (35 and 69 percent of tags deployed, respectively). In these cases, too few fish were captured to meet the tag goals. Factors that influenced catches over the three-year study period included the timing of ice-out, periods of high river discharge, changes in flow patterns at the capture sites, the amount of fishing effort, and differences in relative fish abundance among years. In the Lower River, the timing of ice-out in 2012 was similar to past records, was approximately two weeks late in 2013, and in 2014 was several days earlier than in the past. As a result, the start dates of fishwheel operations in the Lower River varied: May 25 in 2012, June 3 in 2013, and May 22 in 2014. Nonetheless, the number of Chinook Salmon captured in the Lower River among years was similar and ranged from 1,916 (2012) to 2,063 (2013) fish. On the Yentna River, catches of Chinook Salmon increased from 2,295 fish in 2013 to 3,033 fish in 2014. In the Middle River, the same two fishwheel sites (sites 1 and 2) used during the 1980s studies were used successfully from 2012 to 2014. Due to changes in flow patterns observed in 2013 that made it difficult to operate the Site 1 fishwheel when river discharge decreased below 17,500 cfs at the Gold Creek gauge, a third fishwheel site (Site 3) was added to increase catch rates. The addition of a third fishwheel increased fishing effort in 2013 (starting July 17) and 2014 relative to 2012. A substantial portion of catches in 2014 was attributed to the Site 3 fishwheel (30 percent or greater capture of each salmon species). Despite increasing fishing effort in the Middle River, catch in 2014 was lower than in 2013 for all salmon species, but higher than in 2012 for all species except Chum Salmon. Apart from occasional temporary shut downs due to high river discharge, fishing effort across all locations was nearly continuous each season. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 34 October 2015 Fishwheel effectiveness was assessed using fixed-site sonar (DIDSON in 2012/2014, ARIS in 2013) at one of the Middle River fishwheels (Site 1) in each study year. Sonar data confirmed that the fishwheel sampling adequately covered the leading edge of the Chinook Salmon run in June and a representative portion of the run was captured in July. In September, the sonar data were useful for quantifying the relative abundance of passing fish (i.e., Chum, Coho, and Sockeye salmon), particularly when river discharge and turbidity decreased and the fishwheels could not be operated effectively at any of the sites. Under these river conditions, sonar counts were useful for informing Coho Salmon tag rates (e.g., for gillnetting in 2014). Sonar was also used to assess the behavior of fish as they approached the Site 1 fishwheel. Sonar imagery collected at Site 1 showed that the vast majority of fish migrated upstream within 13 m (43 ft) of shore. As a result, lead nets were hung between the shore-side pontoons of the Middle River fishwheels and the adjacent riverbank to increase catch rates (particularly at Site 1 where the fishwheel was held up to 50 ft offshore with spar poles at times). Information on diel pattern obtained from the Site 1 sonar data were useful for informing the crew as to the best times to fish in order to achieve optimal catch rates. Limitations of using sonar to evaluate fishwheel effectiveness were evident in July and August when large numbers of fish and multiple species were present. Under these conditions it took a substantial amount of effort to review the imagery, and apart from large Chinook Salmon, fish observed in the sonar imagery could not be identified by species. Fishwheel effectiveness was difficult to assess during this period, however, by this time protocols for operating the fishwheels under a range of river conditions had been established and there was not likely anything more that could be done to increase fishwheel effectiveness. For all five species of salmon, 2014 catches at the Middle River fishwheels were lower than in 2013, despite a third fishwheel being operated for the entire 2014 season (Table A-8). Except for Chum Salmon, 2014 salmon catches were higher than in 2012. By excluding 2014 catch years at the Site 3 fishwheel and equalizing effort for comparison across years, a different pattern emerges. Fewer Chinook, Chum, Coho, and Pink salmon were captured in 2014 than in 2013 and 2012. Reviewing total catch of Chinook Salmon in the Middle River fishwheels across years showed that 2013 was the highest (952 fish), followed by 2014 (877 fish) and 2012 (566 fish). High catches in 2013 were largely due to the abundance of small Chinook Salmon (336 fish or 55 percent of the catch). More large Chinook Salmon were captured in 2014 (672 fish) than in 2012 (422 fish) or 2013 (616 fish). The majority of Chinook Salmon were captured at the Site 1 fishwheel in 2012 (60 percent) and 2013 (82 percent), whereas only 41 percent of catch occurred at Site 1 in 2014. Operating a third fishwheel (at Site 3) throughout the entire run in 2014 increased overall catch (48 percent of Chinook Salmon were caught at Site 3). Over eight years of operation (1981–1985 and 2012–2014), catch of Chinook Salmon at the Middle River fishwheels was highest in 1984 (1,589 fish) and lowest in 1981 (284 fish; Table A-8; Figure A- 13). Catches in 2012, 2013, and 2014, ranked 7th, 4th, and 5th highest among these years, respectively. Chum Salmon catch at the Middle River fishwheels in 2014 (1,469 fish) was similar to 2012 (1,734 fish) but was 57 percent lower than in 2013 (3,417 fish; Table A-8; Figure A-14). The STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 35 October 2015 2014 Chum Salmon catch was also similar to annual average catch from the 1980s (1,881 fish), but during that period annual catch varied more widely (range: 861–4,228 fish). In 2014, Coho Salmon catch of 335 fish was greater than the average from the 1980s (average = 211 fish) and 2012 total catch (265 fish; Table A-8; Figure A-15); however, the 2014 catch was more than five times lower than 2013 (1,723 fish). Compared with other even years, the 2014 catch of Pink Salmon (7,473 fish) was higher than in 1982 (7,302 fish) and 2012 (4,705 fish), but substantially lower than catch in 1984 (17,394 fish; Table A-8; Figure A-16). From 2012 to 2014, annual catch of Sockeye Salmon at the Middle River fishwheels was among the lowest on record (Table A-8). In 2012, 2013, and 2014, a total of 100, 276, and 223 Sockeye Salmon, respectively, were captured at the Middle River fishwheels. In the 1980s, annual catches averaged 307 fish (range: 161–469 fish; Table A-8; Figure A-17). 5.1.2. Numbers and Size of Marked and Unmarked Fish Recovered at Selected Locations 5.1.2.1. Deshka River Weir An estimated 13,908 Chinook Salmon measuring 50 cm (19.7 in) METF or greater passed the Deshka River weir during May 19 to September 2, 2014, out of a total count of 16,335 Chinook Salmon of all sizes (Table B-1). One hundred twenty-five, or 0.9 percent, were radio-tagged fish and were assumed to have spawned above the weir (Table B-1). The mean length of radio- tagged Chinook Salmon released in the Lower River (69.9 cm [27.5 in]) was larger than that of Chinook Salmon (METF ≥ 50 cm [19.7 in]) that passed the Deshka River weir (67.9 cm [26.7 in]; Table B-3). A total of 11,578 Coho Salmon were counted at the Deshka Creek weir during July 4 to September 2, 2014, all of which were estimated to be 40 cm (15.7 in) METF or greater (Table B- 1). Sixty-eight, or 0.59 percent, were radio-tagged fish and were assumed to have spawned above the weir. The mean length of radio-tagged Coho Salmon released in the Lower River (52.6 cm [20.7 in]) was smaller than that of Coho Salmon (METF ≥ 40 cm [15.7 in]) that passed the Deshka River weir (55.8 cm [22.0 in]; Table B-3). 5.1.2.2. Montana Creek Weir An estimated 1,212 Chinook Salmon measuring 50 cm (19.7 in) or greater passed the Montana Creek weir during June 4 to September 21, 2014, out of a total count of 1,217 Chinook Salmon of all sizes (Table B-2). Fifteen, or 1.2 percent, were radio-tagged fish (Table B-2) and were assumed to have spawned above the weir. The mean length of radio-tagged Chinook Salmon released in the Lower River (69.9 cm [27.5 in]) was slightly larger than Chinook Salmon (METF ≥ 50 cm) that passed the Montana River weir (68.7 cm [27.0 in]; Table B-3). A total of 934 Coho Salmon were counted at the Montana Creek weir during August 3 to September 21, 2014, all of which were estimated to be 40 cm (15.7 in) METF or greater (Table B-2). Four, or 0.43 percent, were radio-tagged fish and were assumed to have spawned above STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 36 October 2015 the weir (Table B-2). The mean length of radio-tagged Coho Salmon released in the Lower River (52.6 cm [20.7 in]) was smaller than that of Coho Salmon (METF ≥ 40 cm [15.7 in]) that passed the Montana Creek weir (56.6 cm [22.3 in]; Table B-3). 5.1.2.3. Middle Fork Chulitna River Sonar An ARIS sonar was operated on the Middle Fork Chulitna River (Figure 3-1) from June 20 to August 3, 2014. Post-season analysis of echograms resulted in a total count of 772 Chinook Salmon measuring 50 cm (19.7 in) METF or greater. Forty-two Chinook Salmon, or 5.4 percent, were radio-tagged fish. Many of the sonar echograms were of poor quality, the total count seemed unusually low, and fish lengths estimated from the sonar appeared suspiciously large, and the data were not used further. It was determined post-season that improper focusing occurred during data collection. 5.1.2.4. Indian River Weir The underwater video system at the Indian River weir was operated 24 hours a day, and collected 89 hours of video footage from 1:30 P.M. on June 22 to 6:29 A.M. on June 26, 2014 (Table B-4). Due to poor visibility, 5.2 hours of video imagery collected on June 26 was not reviewed. Persistent rain on June 25 and June 26 contributed to high-water conditions in the Susitna (Figures 5.1-1 and 5.1-2) and Indian rivers. At approximately 6:29 A.M. on June 26, due to high flows and debris loading, the anchoring system failed and the majority of the weir components were flushed approximately one mile down river. A portion of the weir components were retrieved from July 4–8, and the remainder were retrieved on August 15 when water levels were considerably lower. In total, three Rainbow Trout and two Round Whitefish were observed on the video footage, but no adult salmon (Table B-4). 5.1.2.5. Programmatic Summary The combination of Deshka River and Montana Creek weirs provided a robust second -event sample for evaluating sources of potential bias (heterogeneity in probability) of capture for the mainstem Susitna River Chinook and Coho salmon mark-recapture experiments. When size- biased sampling was detected using these two sites for second event sampling, substantial numbers of all size classes of Chinook and Coho salmon in the mainstem Susitna River were detected at these sites, particularly at the Deshka weir site. As such, size-based capture heterogeneity in the first event was reliably detected and the appropriate size-stratification applied to minimize bias when estimating abundance and distribution. In addition to evaluating size, the weir data allowed for an evaluation of the representation of tagging across each run. Radio-tagged Chinook and Coho salmon that were recaptured at Montana and Deshka weir proved to be tagged across the entire time-frame of the marking event, ensuring a non-zero probability of capture for fish tagged both early and late in the run of fish past the tagging site. As the numbers of fish that can be sampled at these two sites is “fixed” at the escapement for these two tributaries, the precision in estimates of spawning abundance and distribution can only be increased by increasing the number of radio-tags deployed during the marking event, or by identifying additional viable second event sampling sites. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 37 October 2015 Operating a floating picket weir and underwater video system in the lower Indian River did not provide reliable second-event sampling for the Middle River tag site. In 2013, the Indian River weir was successfully operated throughout the majority of the Chinook Salmon run; however, the video system was shut down temporarily from July 19–20, and then permanently on August 20, due to high-water events. In 2014, the weir was rendered inoperable due to a high-water event in late June, prior to the onset of the Chinook Salmon run. During each of these high-water events, discharge of the Susitna River at the Tsusena Creek gauge increased to over 20,000 cfs. At low to moderate Indian River discharges, the site was ideal for weir installation and day-to-day access and operation; however, discharge increased substantially during heavy rain and the effectiveness of the site for sampling was diminished. Other tributaries in the Middle River (e.g., Portage Creek) would likely respond to heavy rains in a similar manner as the Indian River. Based on these findings, tributary weirs in the Middle River are not an effective method for assessing the numbers and size of marked and unmarked fish. 5.1.3. Assessing Any Stock- and Size-selective Capture 5.1.3.1. Use of ARIS to Assess Middle River Fishwheel Effectiveness and Fish Approach Behavior Catch-per-unit-effort at the Site 1 ARIS unit (located immediately downstream of the Site 1 fishwheel; see Figures 3-1 and 4.1-1), or the number of targets counted per hour of imagery reviewed, increased over time in June (Figure A-18, Figure A-19). The ARIS CPUE was zero during the first two days of operation (June 3–4) and only increased slightly from June 5 to June 12 (0.0–0.6 fish/hour). The ARIS CPUE showed a moderate increase from June 13 to June 17 (1.8–6.5 fish/hour), but increased substantially afterwards (10.9–37.0 fish/hour from June 18– 25). In September, CPUE at the ARIS showed peaks on September 1 (37.9 fish/hour) and September 7 (39.0 fish/hour), but steadily declined from September 8 through September 28. In June, trends in CPUE at the Site 1 fishwheel were similar to counts at the ARIS unit (Figure A-20). No adult salmon were captured at the Site 1 fishwheel from June 6 to June 12. The fishwheel CPUE was low from June 13 to June 16 (0.1–0.2 fish/hour), and peaked at 1.0 fish/hour on June 17. In September, CPUE at the Site 1 fishwheel decreased dramatically after August 30 (0.9 fish/hour), and remained low (or zero) through September 7 when the fishwheel was stopped for the season (Figure A-20). This trend contrasts with the ARIS CPUE which peaked in early September and remained relatively high through mid-September (Figure A-19). This decrease in CPUE at the Site 1 fishwheel (Figure A-20) appeared to be related to turbidity, which decreased steadily from 66 NTU on August 29 to 21 NTU on September 7 (Figure A-19). Similar patterns observed in ARIS and fishwheel CPUE in June indicated that the ascending limb of the Chinook Salmon run was captured at the Middle River fishwheels. In contrast, the ARIS data suggested low fishwheel catches of Coho Salmon in the first week of September were not representative of abundance. From September 4–7 for example, CPUE at the ARIS ranged from 19.5–39.0 fish/hour with a net upstream count of 57–147 fish per day (during periods when the fishwheel was operational), yet no adult salmon were captured at the Site 1 fishwheel. Low Coho Salmon catches (Figure A-12) at the Site 1 fishwheel from late August through early September were likely due to low turbidity and not to low relative abundance. Anticipating this STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 38 October 2015 condition, the study team switched to gillnet operations to catch and tag Coho for this part of the run. The proportion of targets observed on the Site 1 ARIS that were first detected within the capture range of the Site 1 fishwheel (~ 6 m [19.7 ft]) appeared to be influenced by river discharge. From June 3 to June 15, when fish measuring 50 cm TL (19.7 in) or greater passing Site 1 were likely Chinook Salmon, Susitna River discharges at the Gold Creek gauge were 19,800 cubic feet per second (cfs) or less and only 27 percent of targets were within 6 m (19.7 ft) of the transducer (Figure A-21). From June 16 to June 25, Susitna River discharges were 21,800 cfs or greater, and the proportion of fish passing within 6 m (19.7 ft) of the transducer increased to 47 perce nt. From August 29 to September 30, when most fish measuring 40 cm TL (15.7 in) or greater passing Site 1 were likely Coho Salmon, the vast majority (88 percent) of targets passed the Site 1 ARIS within 6 m of the transducer. These observations suggested that higher river discharge, likely through water velocity, can affect the cross-channel distribution of migrating Chinook Salmon such that they migrate closer to the bank. The effect was likely more pronounced on Coho Salmon due to their shorter body length. During the periods of monitoring with ARIS at Site 1, migrations were lowest in the early part of the day. In June, 58 percent of upstream-moving fish measuring 50 cm TL (19.7 in) or greater (presumably Chinook Salmon) passed the Site 1 ARIS between noon and midnight (Figure A- 22). Fish passage was lowest early in the morning (~1:00–5:00 A.M.). In September, fish passage (presumably mostly Chum and Coho salmon) was more evenly distributed throughout the day, with 58 percent of fish measuring 40 cm TL (15.7 in) or greater passing between 7:00 A.M. and 6:00 P.M. Fish passage in September was lowest early in the morning (~0:00–5:00 A.M.). 5.1.3.2. Bank Orientation & Capture Probability by Spawning Location (Middle River) Results of contingency table tests comparing the bank of capture to the bank where fish were assigned a spawning destination did not indicate that radio-tagged large Chinook (χ2 = 3.3; P = 0.07), Pink (χ2 = 0.1; P = 0.72), or Sockeye (χ2 = 2.7; P = 0.15) salmon were bank-oriented when passing through the Middle River. Too few small Chinook and Chum salmon returned to left- bank spawning areas to reliably evaluate bank orientation with a contingency table. Specifically, 95 percent (n = 21) of Chum Salmon tagged on the left bank (when looking downstream), and 100 percent (n = 82) of Chum Salmon tagged on the right bank, were assigned to right-bank spawning areas. All small Chinook Salmon returned to spawning areas on the right bank regardless of whether they were captured on the left (n = 2) or right (n = 17) bank. Mainstem spawning populations did not appear more vulnerable to recapture than those fish bound for tributaries (χ2 = 0.1; P = 0.71). Of the 34 radio-tagged salmon released and recaptured at the Middle River fishwheels that were subsequently assigned spawning destinations, 29 (85 percent) were assigned to tributaries and five (15 percent) were assigned to mainstem areas. Similar proportions were observed for radio-tagged fish released at the Middle River fishwheels that were never recaptured (87 percent to tributaries and 13 percent to mainstem areas). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 39 October 2015 5.1.3.3. Size-related Comparisons The following subsections summarize the results of comparisons made between cumulative length-frequency distributions of fish captured and sampled in the Lower River, Yentna River, and Middle River. Two-sample KS tests were used to detect significant differences between the distributions. When interpreting the results of KS tests, it was important to consider both the sample sizes of each distribution and the P-value of the test. For example, in a case where the sample sizes for each distribution were very large and a significant test result was obtained, it was possible that the KS test was detecting small differences between the cumulative length- frequency distributions that had little potential to result in any bias. Despite a significant test result in such a case, there may be no biologically meaningful difference between the two cumulative length-frequency distributions. 5.1.3.3.1. Lower River Cumulative length-frequency distributions for all Chinook Salmon captured at the Lower River tagging site showed significant differences between the size distributions of fish caught at the west bank fishwheel, the east bank fishwheel, and mid-river gillnetting sites. Chinook Salmon caught in mid-river gillnets were larger than fish caught in the west bank fishwheel (D = 0.442, P < 0.001) and the east bank fishwheel (D = 0.387, P < 0.001; Figure A-23; Table A-9). Chinook Salmon caught in the west bank fishwheel were smaller than those from the east bank fishwheel (D = 0.111, P < 0.001; Figure A-23; Table A-9). Average lengths (METF) of Chinook Salmon for each capture station were 75.3 cm (29.6 in) for mid-river gillnets, 57.7 cm (22.7 in) for the west bank fishwheel, and 59.5 cm (23.4 in) for the east bank fishwheel. For Chinook Salmon measuring 50 cm (19.7 in) METF or greater, a significant difference was detected between the length distributions of fish captured at the Lower River tagging site and only those fish radio-tagged at that site (D = 0.145, P < 0.001; Figure A-24; Table A-9). This difference was due largely to the tagging strategy used at the site, which called for Chinook Salmon less than 58 cm (22.8 in) METF to be tagged at approximately one-third the rate of Chinook Salmon measuring 58 cm (22.8 in) METF or greater. This tagging strategy was intended to reduce the effects of size-selective capture by the fishwheels. No significant difference was detected between the length distributions of Chinook Salmon measuring 58 cm (22.8 in) METF or greater caught at the Lower River site and those tagged (D = 0.03, P = 0.797; Table A-9). The cumulative length-frequency distributions for all Coho Salmon captured at the Lower River tagging site was significantly different between the west bank fishwheel and the east bank fishwheel (D = 0.083, P = 0.011; Table A-9). For Coho Salmon (METF ≥ 40 cm), the cumulative length-frequency distributions were not significantly different between the total catch and those tagged (D = 0.023, P = 0.886; Table A-9). The cumulative length-frequency distributions for Pink Salmon tagged at the Lower River tagging site were not significantly different between the west bank fishwheel and the east bank fishwheel (D = 0.145, P = 0.14; Table A-9). For Pink Salmon, length data were collected only for fish that were radio-tagged so no further comparisons can be made (Figure A-24). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 40 October 2015 The cumulative length-frequency distribution for Chinook Salmon sampled at the Deshka River weir was not significantly different than that for all radio-tagged fish above the tagging site (D = 0.09, P = 0.101; Figure A-25). The cumulative length-frequency distribution for Chinook Salmon sampled at the Montana Creek weir was significantly different than that for all radio-tagged fish above the tagging site (D = 0.13, P = 0.006; Figure A-25). 5.1.3.3.2. Yentna River Cumulative length-frequency distributions for Chinook Salmon captured at the Yentna RM 6 tagging site showed significant differences between the size distributions of fish caught at the south bank fishwheel, the north bank fishwheel, and mid-river gillnetting sites. Chinook Salmon caught in mid-river gillnets were larger than fish caught in the south bank fishwheel (D = 0.519, P < 0.001) and the north bank fishwheel (D = 0.658, P < 0.001; Figure A-26; Table A-9). Chinook Salmon caught in the north bank fishwheel were smaller than those from the south bank fishwheel (D = 0.155, P < 0.001; Figure A-26; Table A-9). Average lengths (METF) of Chinook Salmon for each capture station were 72.0 cm (28.4 in) for mid-river gillnets, 48.9 cm (19.3 in) for the south bank fishwheel, and 43.2 cm (17.0 in) for the north bank fishwheel. For Chinook Salmon measuring 50 cm (19.7 in) METF or greater, no significant difference was detected between the length distributions of fish captured at the Yentna RM 6 tagging site and only those fish dart-tagged at that site (D = 0.005, P = 1.00; Figure A-27; Table A-9). Cumulative length-frequency distributions for all Chinook Salmon captured at the Yentna RM 18 recapture site also showed significant differences between the size distributions of fish caught at the south bank fishwheel, the north bank fishwheel, and mid-river gillnetting sites. Chinook Salmon caught in mid-river gillnets were larger than fish caught in the south bank fishwheel (D = 0.528, P < 0.001) and the north bank fishwheel (D = 0.671, P < 0.001; Figure A-26; Table A-9). Chinook Salmon caught in the north bank fishwheel were smaller than those from the south bank fishwheel (D = 0.25, P < 0.001; Figure A-26; Table A-9). Average lengths (METF) of Chinook Salmon for each capture station were 77.9 cm (30.7 in) for mid-river gillnets, 59.3 cm (23.3 in) for the south bank fishwheel, and 50.7 cm (20.0 in) for the north bank fishwheel. The cumulative length-frequency distribution for Chinook Salmon sampled at the Yentna RM 18 recapture site was not significantly different than that for all dart-tagged fish above the tagging site (D = 0.036, P = 0.265; Figure A-28). 5.1.3.3.3. Middle River In the Middle River, the cumulative length-frequency distributions for Chum Salmon at site 2 was significantly different from that at site 1 (D = 0.14, P = 0.01) and site 3 (D = 0.15, P < 0.01). Also, Pink Salmon length distributions differed between sites 1 and 3 (D = 0.09, P = 0.04; Figure A-29; Table A-10). Sample sizes were large for these comparisons (n: 342–449) and tagging effort was distributed amongst the sites, so it was unlikely these differences introduced significant bias. Among sites, mean lengths were greatest at Site 2 for Chinook (66.4 cm; 26.2 in), Chum (58.6 cm; 23.1 in), Coho (55.6 cm; 21.9 in), and Sockeye (50.0 cm; 19.7 in) salmon; but greatest at Site 3 for Pink Salmon (46.0 cm; 18.1 in). Mean lengths were smallest at Site 1 for Chum (57.5 cm; 22.7 in), Coho (53.8 cm; 22.7 in), Pink (45.5 cm; 17.9 in), and Sockeye STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 41 October 2015 (48.5 cm; 19.1 in) salmon; but smallest at Site 3 for Chinook Salmon (62.1 cm; 24.5 in). Small Chinook Salmon (METF < 50 cm [19.7 in]) comprised 23 percent of all Chinook Salmon caught in 2014 (versus 35 percent in 2013); and 5 percent of Sockeye Salmon caught in 2014 measured less than 40 cm (15.7 in) METF (versus 36 percent in 2013). Cumulative length-frequency distributions for salmon captured at the Middle River fishwheels were significantly different than those of radio-tagged fish for small Chinook (D = 0.77, P < 0.01) and Pink Salmon (D = 0.15, P < 0.01), but not for large Chinook, Chum, Coho, or Sockeye salmon (Figure A-30; Table A-10). For small Chinook and Pink salmon, these results were consistent with tagging effort as it was not random across all sizes, but instead was limited to the larger-sized segment of these fish captured in the fishwheels. 5.1.3.3.4. Programmatic Summary Achieving the goals of this study was dependent in part on tagging a representative group of fish from the entire population. However, fishwheels have been shown to be size and species selective for adult salmon. Meehan (1961) showed that fishwheels on the Taku River caught a larger proportion of smaller-sized Chinook Salmon compared with samples collected on the spawning grounds. Meehan (1961) also showed that Chinook and Coho salmon were least susceptible to recapture in a fishwheel, while Pink Salmon were most easily recaptured. In 1981 and 1982 on the Susitna River, ADF&G (1983a) compared observed and expected mark rates on the spawning grounds and found that fishwheels operated near Curry were species selective: Chinook and Chum salmon catches were biased low, and Pink Salmon catches were biased high. Meehan (1961) hypothesized that size selectivity was due to larger fish avoiding the fishwheel, or migrating in faster and deeper water away from shore, relative to smaller fish. A tendency for large salmon to swim upstream farther from the bank than smaller ones in locations where the river gradient is low and velocities offshore are modest also has been suggested by ADF&G (1983a) and Hughes (2004). In contrast, there is also evidence showing that fishwheels can catch a representative sample of salmon when deployed in areas where elevated water velo cities force fish to migrate near shore (Link and Nass 1999). KS tests indicated some size selectivity in capture (toward disproportionate capture of smaller- sized fish) in the Lower River fishwheels. Size-selective sampling was detected for Chinook Salmon radio-tagged at the Lower River site in 2013 and 2014, even where capture was achieved using a combination of fishwheels and drift gillnets. Chinook Salmon measuring less than 58 cm (22.8 in) METF were radio-tagged at approximately one-third the rate of larger fish based on an analysis of data from Chinook Salmon tagged in 2012 that passed the Deshka River weir site. These measures were not sufficient to eliminate sampling bias, but they likely reduced over- sampling of small fish. Size-selective sampling was also detected for Coho Salmon radio-tagged at the Lower River site in 2013 and 2014. And lastly, size selectivity was detected in the Yentna River in 2014 for Chinook Salmon. No length data were available for the second sample event in the Yentna drainage in 2013, so size selectivity could not be tested. Furthermore as described above, for cases where size selectivity was detected, size-stratification provided minimally biased estimates of abundance and distribution of spawning Chinook and Coho salmon. In the Middle River, size selectivity could only be directly tested in one study year (2013) due to difficulties in collecting a sufficient number of length samples on the spawning grounds. As a STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 42 October 2015 result, ancillary length-frequency comparisons (i.e., between capture sites, and between fish captured and tagged), sonar data collected immediately below the Site 1 fishwheel, and expert opinion were used to provide some insights into the potential for size selectivity in the Middle River. In the first study year (2012), the study team conducted ground -based stream counts and carcass surveys in the Indian River and Portage Creek to sample fish for mark rates and lengths. Very few carcasses were found despite excellent survey conditions, which was likely due to carcasses being removed from the rivers by predators. In 2013, the study team installed a picket weir and underwater video system on the lower Indian River as a method of obtaining mark rates and length data on the spawning grounds. Comparisons of cumulative length-frequency distributions for large Chinook Salmon radio-tagged in the Middle River and those inspected and recaptured at the weir showed no evidence of size-selective sampling. Too few radio-tagged fish of the other salmon species were recaptured at the weir in 2013 to test for size selectivity. In 2014, the study team could not directly test for size-selective sampling because the Indian River weir was rendered inoperable prior to the onset of the Chinook Salmon run and thus no length samples were collected. For small Chinook and Pink salmon, tagging efforts were not random at the Middle River fishwheels in 2013 and 2014 as only the larger-sized segment of the small fish captured were radio-tagged. This was due to the fact that the radio tags would simply not fit into the stomachs of smaller-sized fish. For Pink Salmon, the difference in mean length between captured and radio-tagged fish was less than 1.5 cm (0.6 in), and relatively few fish captured measured less than 40 cm (15.7 in) METF, so it was unlikely that selecting for slightly larger-sized fish to tag had a material effect on the study results. Small Chinook Salmon, however, comprised a substantial portion of the total number of Chinook Salmon captured at the Middle River fishwheels (23–35 percent in 2013 and 2014), yet only a fraction were radio-tagged (11 percent or less). In 2014, the smallest radio-tagged Chinook Salmon measured 36 cm (14.2 in) METF, yet 61 percent (121 of 198 fish) of small Chinook Salmon captured measured less than 36 cm (14.2 in) METF. No radio-tagged small Chinook Salmon passed above Devils Canyon in the three study years, so it was unlikely that any additional fish would have passed the impediments had additional small Chinook Salmon been radio-tagged. Additional information suggested size-selectivity did not introduce significant bias to study results. Sonar data collected immediately downstream of the Site 1 fishwheel in the Middle River suggested that very few fish migrated upstream at distances greater than 13 m (42.7 ft) from the shore-based transducer, and that fish of all sizes seemed equally distributed at range from shore. Sonar was not used at sites 2 or 3, however, the higher water velocities at these sites suggested an even greater proportion of fish would be bank-oriented relative to Site 1. All size classes of salmon were captured at each fishwheel, indicating that fish of all lengths had a non- zero capture probability. And lastly, based on the experience of the study team, the Middle River fishwheel sites were characterized as generally high gradient with moderate-to-high offshore river velocities, which were similar features of fishwheel sites on other river systems where the least size selectivity was encountered (Link and Nass 1999; Smith et al. 2005). 5.1.4. Examining Handling-Induced Changes in Behavior Fish that were obviously affected by handling (those that moved only in a downstream direction, and those that never moved) were not included in any of the behavioral analyses described in this STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 43 October 2015 report. Regardless, other fish may have also experienced handling-related changes in behavior. One way to quantify handling-induced effects was to compare the survival and behavior of fish that were handled twice (i.e., recaptured in fishwheels) to those of the general populations of tagged fish, specifically, comparing the proportions tracked to spawning destinations. Of the 590 large Chinook Salmon that were radio-tagged and released at the Middle River fishwheels, 21 (3.6 percent) fish were recaptured at least once at the Middle River fishwheels, of which two fish were recaptured a second time. Most of the large Chinook Salmon recaptured for the first time were caught at the Site 3 fishwheel (14 fish), which was located the farthest upstream, while the remainder were caught at the Site 1 (6 fish) and Site 2 (1 fish) fishwheels. The elapsed time between tag and recapture events ranged from 14 minutes to 21 days (median = 4.1 days; n = 17). The fish recaptured 21 days after release was noted as a ‘post-spawn’ fish by the crew. Of the 21 fish recaptured at least once, the radio tag was coded for 17 fish. Of these 17 recaptured fish, 53 percent were subsequently assigned spawning destinations (i.e., classified into a specific tributary or mainstem spawning location); this percentage was low compared to the overall rate of 80 percent (χ2 = 7.4; P < 0.01). Neither of the two fish recaptured twice were assigned spawning destinations. The lower proportion of assigned destinations and relatively long times between capture events suggested that the behavior of large Chinook Salmon handled twice may have been negatively influenced by the recapture events, thus failing to dismiss the possible impacts of the original capture event Three (9.4 percent) of the 32 radio-tagged small Chinook Salmon released at the Middle River fishwheels were recaptured (median time between capture events was 13.3 days; n = 3), all of which were assigned spawning destinations, suggesting that the additional handling did not substantially influence the behavior of these fish. The negligible impacts of second handling events suggest that the original handling of these fish was benign. Ten Pink, 8 Chum, 5 Coho, and 4 Sockeye salmon released at the Middle River fishwheels were also recaptured. The radio tag was not coded upon recapture for one Chum and one Pink salmon. Of the recaptured fish with known tag codes, the elapsed time between capture events was longest for Sockeye (median = 1.4 days, n = 4), followed by Chum (median = 0.9 days, n = 7), Pink (median = 0.9 days, n = 9), and Coho (median = 0.8 days, n = 5) salmon. Seventy-one percent of recaptured Chum Salmon were assigned spawning destinations, which was similar to the overall rate of 80 percent (χ2 = 0.3; P = 0.60). All of the recaptured Pink, Sockeye, and Coho salmon were assigned spawning destinations. The relatively short time period between capture events and the large proportion of recaptured fish that were assigned spawning destinations suggests the additional handling did not substantially influence the behavior of Chum, Coho, Pink, or Sockeye salmon. Moreover, it was assumed that the short recapture period indicated the fish had sufficiently recovered from the first handling event, having rejoined the migration of untagged fish. The negligible impacts of second handling events suggest that the original handling of these species was benign. Another way to quantify handling-induced effects was to examine swim speeds in a given river reach, comparing newly tagged fish to those that have had time to recover from handling. Few fixed-station receivers monitored both Lower and Middle River frequencies, hence the best test was to compare travel speeds of Lower-River-tagged fish from Sunshine to Lane to those of Middle-River-tagged fish from release (near Curry) to Gateway. If the newly tagged fish were STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 44 October 2015 experiencing handling-induced effects, their travel speeds should have been slower than those of nearby Lower-River-tagged fish. In all cases, the recently-handled Middle-River-tagged fish travelled faster than the unhandled (Lower-River-tagged) conspecifics (Figure 5.1-3), with effects being statistically significant for Coho Salmon (χ2 = 9.7, P = 0.0018; Chinook Salmon: χ2 = 0.8, P = 0.77; Pink Salmon: χ2 = 3.5, P = 0.061). As a comparison among tagging sites of the relative magnitude of potential handling-induced effects, the proportion of fish that either never left the release area or that moved only in a downstream direction was examined. For large Chinook Salmon, the proportion was 4 percent at the Lower River fishwheels, 10 percent at the Middle River fishwheels, and 19 percent in the Yentna River. For Coho Salmon, the proportion was 1 percent at the Lower River fishwheels, 11 percent at the Middle River fishwheels, and 12 percent in the Yentna River. For Pink Salmon, the proportion was 10 percent at the Lower River fishwheels, and 3 percent at the Middle River fishwheels. 5.2. Objective 2: Determine the migration behavior and spawning locations of radio-tagged fish in the Lower, Middle, and Upper Susitna River Migration behavior and spawning locations were relatively consistent among years. Of the fish tagged in the Lower River that could be categorized to a destination, the majority (97–99 percent of the large Chinook Salmon, 90 percent of the Chum Salmon, 93–97 percent of the Coho Salmon, 84–99 percent of the Pink Salmon, and 100 percent of the Sockeye Salmon) had tributary destinations, rather than mainstem ones. The Talkeetna, Yentna, Deshka, and Chulitna rivers were the main tributary destinations. For the fish tagged in the Middle River a similar pattern was observed, showing predominant use of tributaries for all species (tributaries were used by 90–94 percent of the large Chinook Salmon, 83–93 percent of the small Chinook Salmon, 76–90 percent of the Chum Salmon, 84–94 percent of the Coho Salmon, and 91–94 percent of the Pink Salmon) except Sockeye Salmon. Sockeye Salmon tagged in the Middle River had the greatest tendency to have a mainstem destination (only 21–54 percent had tributary destinations), which was markedly different behavior from their Lower River conspecifics, and from all other salmon species. For fish tagged in the Middle River, Portage Creek and Indian River were the main tributary destinations for all species (AEA 2013a, 2014c). Detailed results related to the migration behavior and spawning locations of radio-tagged fish in 2014 are presented below and in Appendices D and E. Destinations and potential spawning sites for radio-tagged fish across years are shown in Figures D-1 to D-20. 5.2.1. Chinook Salmon 5.2.1.1. Fish tagged in Lower River 5.2.1.1.1. Tag Returns Of the large Chinook Salmon radio tags deployed in the Lower River, 24 were recovered by anglers (23 tags) or project field staff (1 tag; Table E-1). Large Chinook Salmon tags were recovered in the Chulitna (1), Deshka (16), and Lower Susitna (1) rivers, as well as Clear (2), Deception (1), Lake (1), Peter’s (1), and Sheep (1) creeks. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 45 October 2015 5.2.1.1.2. Stock Classifications and Spawning and Holding Locations Of the 656 large Chinook Salmon tagged in the Lower River, 581 (89 percent) were classified by destination (Table 5.2-1). Of the remaining 75 tags, 44 were ignored (zero or one detection, never moved from release site, or moved only in the downstream direction) and 31 exhibited movements that prevented conclusive assignment to the mainstem or tributaries (see “Other Classifications” in Table 5.2-1). Of the 581 Chinook Salmon tracked to a destination, 7 (1 percent) went to destinations in the mainstem Susitna River (Table 5.2-1; Figure 5.2-1). There were six potential spawning and holding sites documented in the Lower River, including 3 within slough/side channel, 2 within main channel, and 1 within tributary mouth habitats. There were no potential spawning sites identified in the Middle River downstream or upstream of Devils Canyon, but there was one potential mainstem spawning site within Devils Canyon, located at the mouth of Cheechako Creek. Spawning was not confirmed at any of the potential spawning sites for Chinook Salmon. Lack of confirmation was largely due to high turbidity precluding visual observation. Of the 581 Chinook Salmon tracked to a destination, 574 (99 percent) went to tributaries. Of these, 94.6 percent used Lower River tributaries, mainly the Yentna, Deshka, Talkeetna or Chulitna rivers (Figure 5.2-2). The remaining 5.4 percent had tributary destinations in the Middle River Segment below Devils Canyon, mainly in Indian River or Portage Creek. One fish had a tributary destination above Impediment 1, in Cheechako Creek. 5.2.1.1.3. Roaming Behavior in the Middle River Of the large Chinook Salmon tagged in the Lower River that entered the Middle River, 13.9 percent of the fish with known-classification subsequently moved downstream into a tributary (Table 5.2-2). Roamers eventually went to either the Chulitna or Talkeetna rivers, or Montana Creek. Some roaming fish moved far up into the Middle River before dropping back to enter downstream tributaries (Table 5.2-3). For example, one Chinook Salmon tagged in Lower River moved upstream to the area below Impediment 1 before dropping back to enter the Chulitna River. 5.2.1.2. Fish Tagged in Yentna River 5.2.1.2.1. Tag Returns Of the large Chinook Salmon radio tags deployed in the Yentna River, four were recovered by anglers (Table E-1). Large Chinook Salmon tags were recovered in the Yentna at the mouth of Lake Creek (2), or in Lake Creek itself (2). 5.2.1.2.2. Stock Classifications and Spawning Locations Chinook Salmon radio-tagged in the Yentna River were expected to stay within this major tributary, and significant movement to other Susitna River tributaries was not expected (relative to Chinook Salmon tagged in the Lower River). As expected, 219 of the 295 Chinook Salmon released in the Yentna River (74 percent) were classified with a Yentna destination, and 8 (3 percent) were classified in other Lower Susitna River tributaries (Little Willow and Willow STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 46 October 2015 creeks, or Deshka or Chulitna rivers; Table 5.2-1). Of the remaining 68 tags, 64 were ignored (zero or one detection, never moved from release site, or moved only in the downstream direction) and four exhibited movements that prevented conclusive assignment to the mainstem or tributaries (see “Other Classifications” in Table 5.2-1). 5.2.1.2.3. Roaming Behavior in the Middle River No fish tagged in the Yentna River migrated into the Middle River and subsequently moved downstream into a tributary. 5.2.1.3. Fish Tagged in Middle River 5.2.1.3.1. Tag Returns Of the large Chinook Salmon radio tags deployed in the Middle River, thirteen were recovered by anglers (3 tags), project field staff (6 tags), or others (4 tags; Table E-2). Thirteen large Chinook Salmon tags were recovered in the Indian (5) and Middle Susitna (Indian River confluence; 1) rivers, at the mouth of Jack Long Creek (1), and at the Site 3 fishwheel (6). 5.2.1.3.2. Stock Classifications and Spawning Locations Of the 590 large Chinook Salmon radio-tagged in the Middle River, 472 (80 percent) were classified by spawning destination (Table 5.2-1). Of the remaining 118 tags, 58 were ignored (zero or one detection, never moved from release site, or moved only in the downstream direction) and 60 exhibited movements that prevented conclusive assignment to the mainstem or tributaries (Table 5.2-1). Of the 472 large Chinook Salmon tracked to a spawning and holding destination, 34 (7 percent) went to mainstem destinations, all in the Middle River downstream of Devils Canyon (Table 5.2- 1; Figure 5.2-1; Table D-2), including 8 within slough/side channel, 4 within main channel, and 22 within tributary mouth habitats. The other 438 fish (93 percent) went to tributaries. Of these, 11 percent used Lower River tributaries (mainly Talkeetna or Chulitna rivers; Figure 5.2-3), 88.4 percent used tributaries in the Middle River below Devils Canyon (mainly Indian River or Portage Creek; Figure 5.2-3), 2 fish (0.5 percent) used a tributary within the canyon (Cheechako Creek), and 1 fish (0.2 percent) used an Upper River tributary (Kosina Creek). In addition to the large Chinook Salmon described above, 32 small Chinook Salmon were radio- tagged and released in the Middle River. For these small Chinook Salmon, 24 (75 percent) were classified by destination (Table 5.2-1). Of the remaining 8 small Chinook Salmon tagged, 5 were ignored (never moved from release site, or moved only in the downstream direction) and 3 exhibited movements that prevented conclusive assignment to the mainstem or tributaries (see “Other Classifications” in Table 5.2-1). Of the 32 small Chinook Salmon tracked to a spawning destinations, 4 (17 percent) went to destinations in the mainstem Middle River downstream from Devils Canyon (Table 5.2-1; Table D-2; Figure 5.2-1): 3 were at the mouth of Indian River, and one was in a side channel habitat. The other 20 fish (83 percent) went to tributaries Of these, 1 fish (5 percent) used a Lower River STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 47 October 2015 tributary (Talkeetna River; Figure 5.2-3), and the remaining 19 fish (95 percent) used tributaries in the Middle River below Devils Canyon (mainly Indian River or Portage Creek; Figure 5.2-3). Chinook Salmon (small and large) were tracked to 18 potential mainstem spawning and holding sites in the Middle River between PRM 111.0 and PRM 155.9 (Table D-2). There were five sites at which more than one radio-tagged Chinook Salmon was detected (#3, 10, 13, 15, 17). No potential mainstem spawning sites for Chinook Salmon were identified above PRM 160. To assess if there was spawning activity, all 18 sites were visually examined during aerial telemetry, boat, or foot surveys (Habitat Suitability Criteria [HSC]; Table D-1); and turbid water precluded visual confirmation of spawning activity at all sites. A DIDSON unit was used at six of the sites; however the remaining 12 sites were not surveyed using sonar due to lack of boat access or bathymetric features that were not conducive to sonar sampling. Holding behavior was observed at one main channel (#10) and three tributary mouth habitats (#5, 13, 17), but no fish were observed spawning at any of the 18 sites. 5.2.1.3.3. Roaming Behavior in the Middle River Several fish tagged at the Curry fishwheels moved downstream into a Lower or Middle River tributary (Table 5.2-2). For large Chinook Salmon tagged at the Curry fishwheels, 472 had known classifications, of which 54 (11.4 percent) exhibited this roaming behavior. Most roamers eventually went to either the Chulitna or Talkeetna rivers. Some roaming fish moved far up into the Middle River before dropping back to enter downstream tributaries (Table 5.2-3). For example, two Chinook Salmon tagged in Middle River moved upstream to the area below Impediment 1 before dropping back to enter the Talkeetna River. For small Chinook Salmon tagged at the Curry fishwheels, 24 had known classifications of which 5 (20.8 percent) exhibited roaming behavior. The farthest upstream any of these fish reached was the Gateway site, and the farthest downstream tributary was Talkeetna River. 5.2.2. Chum Salmon 5.2.2.1. Fish Tagged in Middle River 5.2.2.1.1. Tag Returns Of the Chum Salmon radio tags deployed in the Middle River, one was recovered by field crew at the mouth of 4th of July Creek (Table E-2). 5.2.2.1.2. Stock Classifications and Spawning Locations Of the 200 Chum Salmon tagged in the Middle River, 159 (80 percent) were classified by destination (Table 5.2-1). Of the remaining 41 tags, 10 were ignored (zero or one detection, never moved from release site, or moved only in the downstream direction) and 31 exhibited movements that prevented conclusive assignment to the mainstem or tributaries (Table 5.2-1). Of the 159 Chum Salmon tracked to a spawning destination, 18 (11 percent) went to destinations in the mainstem Middle River (Table 5.2-1; Table D-3; Figure 5.2-1), including 11 within slough/side channel, 2 within main channel, and 5 within tributary mouth habitats. The other 141 (89 percent) went to tributaries. Of these, 30.5 percent used Lower River tributaries (mainly STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 48 October 2015 Talkeetna or Chulitna rivers; Figure 5.2-3), and 69.5 percent used tributaries in the Middle River below Devils Canyon (mainly Indian River or Portage Creek; Figure 5.2-3). Chum Salmon were tracked to 14 potential mainstem spawning and holding sites in the Middle River between PRM 111.3 and PRM 155.9 (Table D-3). There were two sites at which more than one radio-tagged Chum Salmon was detected (#7, 9). No potential mainstem spawning sites for Chum Salmon were identified above PRM 152.3. To assess if there was spawning activity, all 14 sites were visually examined during aerial telemetry, boat, or foot surveys. Eleven sites were surveyed aerially (#1–6, 8, 10–14), and ten sites by boat/foot (#2, 5–13). No fish were observed at three of the sites (#3, 4, 7; Table D-3). Six sites were too turbid to see fish during aerial surveys (#1, 2, 6, 10, 11, 13) even though they were regularly monitored. Holding behavior was observed at two slough/side channel (#5, 9) and three tributary mouth habitats (#8, 12, 14), while fish were observed spawning at two of the 14 sites (#5, 12). Both confirmed spawning sites were also confirmed in 2012 and 2013. 5.2.2.1.3. Roaming Behavior in the Middle River Several fish tagged at the Curry fishwheels moved downstream into a Lower or Middle River tributary (Table 5.2-2). Of the classified Chum Salmon tagged at the Curry fishwheels, 28 percent returned to a downstream tributary. Chum Salmon roamed as far as the mouth of Portage Creek before turning downstream. Most of the roamers eventually went to either the Chulitna or Talkeetna rivers, and three went as far as Montana Creek. 5.2.3. Coho Salmon 5.2.3.1. Fish Tagged in Lower River 5.2.3.1.1. Tag Returns Of the Coho Salmon radio tags deployed in the Lower River, 36 were recovered by anglers (35 tags) or project field staff (1 tags; Table E-1). Coho Salmon tags were recovered in the Deshka (9), Kashwitna (2), Talachulitna (1), and Talkeetna (6) rivers; and Clear (4), Little Willow (1), Montana (2), Rabideaux (2), Sheep (1), Sunshine (2), Trapper (2), Troublesome (1), Whiskers (1), and Willow (2) creeks. 5.2.3.1.2. Stock Classifications and Spawning Locations Of the 640 Coho Salmon tagged in the Lower River, 581 (91 percent) were classified by destination (Table 5.2-1). Of the remaining 59 tags, 10 were ignored (zero or one detection, never moved from release site, or moved only in the downstream direction) and 49 exhibited movements that prevented conclusive assignment to the mainstem or tributaries (see “Other Classifications” in Table 5.2-1). Of the 581 Coho Salmon tracked to a destination, 16 (3 percent) went to destinations in the mainstem Susitna River (Table 5.2-1; Figure 5.2-1). All sixteen potential spawning and holding sites were in the Lower River. These sites included 13 slough/side channel and 3 tributary mouth habitats. During the survey period, mainstem water clarity was ideal for visual STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 49 October 2015 confirmation of spawning activity; however, none of the potential spawning sites were confirmed for spawning. Of the 581 Coho Salmon tracked to a destination, 565 (97 percent) went to tributaries. Of these, 94.2 percent used Lower River tributaries, mainly the Yentna, Deshka, Talkeetna, or Chulitna river (Figure 5.2-2). The remaining 5.8 percent had tributary destinations in Middle River downstream from Devils Canyon, mainly in Whiskers Creek or Indian River. 5.2.3.1.3. Roaming Behavior in the Middle River Of the Coho Salmon tagged in the Lower River that entered the Middle River, 40 percent of the fish with known-classification subsequently moved downstream into a tributary (Table 5.2-2). Most roamers eventually went to Chase or Whiskers creeks, or Chulitna River. Roaming fish moved as far up into the Middle River as the mouth of Indian River before dropping back to enter downstream tributaries (Table 5.2-3). 5.2.3.2. Fish Tagged in Yentna River 5.2.3.2.1. Tag Returns Of the Coho Salmon radio tags deployed in the Yentna River, two were recovered (Table E-1), both from Deshka River. 5.2.3.2.2. Stock Classifications and Spawning Locations Coho Salmon radio-tagged in the Yentna River were expected to stay within this major tributary, and significant movement to other Susitna River tributaries was not expected (relative to Coho Salmon tagged in the Lower River). As expected, 43 of the 60 Coho Salmon released in the Yentna River (72 percent) were classified with a Yentna destination, and 5 (8 percent) were classified in other Lower Susitna River tributaries (Deshka or Chulitna rivers; Table 5.2-1). The remaining 12 tags were ignored (zero or one detection, never moved from release site, or moved only in the downstream direction; see “Other Classifications” in Table 5.2-1). 5.2.3.2.3. Roaming Behavior in the Middle River No fish tagged in the Yentna River migrated into the Middle River and subsequently moved downstream into a tributary. 5.2.3.3. Fish Tagged in Middle River 5.2.3.3.1. Tag Returns Of the Coho Salmon radio tags deployed in the Middle River, two were recovered: one in the Talkeetna River and one in Portage Creek (Table E-2). 5.2.3.3.2. Stock Classifications and Spawning Locations Of the 230 Coho Salmon tagged in the Middle River, 184 (80 percent) were classified by destination (Table 5.2-1). Of the remaining 46 tags, 26 were ignored (zero or one detection, STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 50 October 2015 never moved from release site, or moved only in the downstream direction) and 20 exhibited movements that prevented conclusive assignment to the mainstem or tributaries (Table 5.2-1). Of the 184 Coho Salmon tracked to a spawning destination, 11 (6 percent) went to mainstem destinations: one in the Lower River (a side channel near the mouth of the Chulitna), and ten to the Middle river (Table 5.2-1; Table D-4; Figure 5.2-1), including 5 within slough/side channel, 2 within main channel, and 3 within tributary mouth habitats. The other 173 fish (94 percent) went to tributaries. Of these, 15 percent used Lower River tributaries (mainly Talkeetna River; Figure 5.2-3), and 85 percent used tributaries in the Middle River downstream from Devils Canyon (mainly Indian River or Portage Creek; Figure 5.2-3). Coho Salmon were tracked to eight potential mainstem spawning and holding sites in the Middle River between PRM 111.3 and PRM 155.9 (Table D-4). There were two sites at which more than one radio-tagged Coho Salmon was detected (#3, 6). No potential mainstem spawning and holding sites for Coho Salmon were identified above PRM 152.3. To assess if there was spawning activity, seven sites were visually examined during aerial telemetry, boat, or foot surveys (HSC). Seven sites were surveyed aerially (#1–6, 8), and two sites by boat/foot (#2, 7). No fish were observed at five of the sites (#3-7; Table D-4). Two sites were too turbid to see fish during aerial surveys (#1, 2) even though they were regularly monitored. Holding behavior was observed at one tributary mouth habitat (#8), but no fish were observed spawning at any of the eight sites (despite ideal water clarity for visual inspection). 5.2.3.3.3. Roaming Behavior in the Middle River Several fish tagged at the Curry fishwheels moved downstream into a Lower or Middle River tributary (Table 5.2-2). For Coho Salmon tagged at the Curry fishwheels, 29 percent of the classified fish exhibited this roaming behavior. Middle River Coho Salmon roamed as far as the mouth of Portage Creek before turning downstream, and one fish moved far enough downstream to enter Yenta River. 5.2.4. Pink Salmon 5.2.4.1. Fish Tagged in Lower River 5.2.4.1.1. Tag Returns Of the Pink Salmon radio tags deployed in the Lower River, two were recovered (Table E-1): one from Willow Creek and one from Montana Creek. 5.2.4.1.2. Stock Classifications and Spawning Locations Of the 199 Pink Salmon tagged in the Lower River, 156 (78 percent) were classified by destination (Table 5.2-1). Of the remaining 43 tags, 20 were ignored (never moved from release site) and 22 exhibited movements that prevented conclusive assignment to the mainstem or tributaries (see “Other Classifications” in Table 5.2-1). Of the 156 Pink Salmon tracked to a destination, only 1 fish (< 1 percent) was classified as having a destination in the mainstem Susitna River (Table 5.2-1; Figure 5.2-1). It was a main STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 51 October 2015 channel site in the Lower River. Spawning was not confirmed at this location due to high turbidity precluding visual observation. Of the 156 Pink Salmon tracked to a destination, 155 (~99 percent) went to tributaries. Of these, 97.4 percent used Lower River tributaries, mainly the Yenta, Deshka or Chulitna rivers, or Willow Creek (Figure 5.2-2). The remaining 2.6 percent (4 fish) had tributary destinations in Middle River below Devils Canyon, specifically, in 4th of July Creek or Indian River. 5.2.4.1.3. Roaming Behavior in the Middle River Of the Pink Salmon tagged in the Lower River that entered the Middle River, 33 percent of the fish with known-classification subsequently moved downstream into a tributary, either to the Talkeetna or Chulitna rivers. These Pink Salmon roamed as far as the mouth of Portage Creek before turning downstream. 5.2.4.2. Fish Tagged in Middle River 5.2.4.2.1. Tag Returns Of the Pink Salmon radio tags deployed in the Middle River, two were recovered: one in 4th of July Creek, and one at the Montana Creek weir (Table E-2). 5.2.4.2.2. Stock Classifications and Spawning Locations Of the 201 Pink Salmon tagged in the Middle River, 176 (88 percent) were classified by destination (Table 5.2-1). Of the remaining 25 tags, 8 were ignored (zero or one detection, never moved from release site, or moved only in the downstream direction) and 17 exhibited movements that prevented conclusive assignment to the mainstem or tributaries (Table 5.2-1). Of the 176 Pink Salmon tracked to a spawning destination, 12 (7 percent) went to destinations in the mainstem Middle River (Table 5.2-1; Table D-5; Figure 5.2-1), including 5 with slough/side channel, and 7 with tributary mouth habitats. The other 164 fish (93 percent) went to tributaries. Of these, 23.9 percent used Lower River tributaries (mainly Talkeetna or Chulitna rivers; Figure 5.2-3), and 67.1 percent used tributaries in the Middle River downstream from Devils Canyon (mainly 4th of July Creek or Indian River; Figure 5.2-3). Pink Salmon were tracked to eight potential mainstem spawning and holding sites in the Middle River between PRM 111.3 and PRM 155.9 (Table D-5). There were three sites at which more than one radio-tagged Pink Salmon was detected (#1, 3, 7). No potential mainstem spawning and holding sites for Pink Salmon were identified above PRM 148. To assess if there was spawning activity, all eight sites were visually examined during aerial telemetry, boat, or foot surveys (HSC). Four sites were surveyed aerially (#1, 5, 6, 8), and four sites by boat/foot (#1, 5, 6, 8). No fish were observed at two of the sites (#2, 4). Three sites were too turbid to see fish during aerial surveys (#5, 6, 8) even though they were regularly monitored. Holding behavior was observed at two tributary mouth habitats (#3, 7), but no fish were observed spawning at any of the 8 sites (despite that water clarity was ideal for visual inspection). The two potential spawning sites that Pink Salmon were observed holding in 2014 had been spawning locations in at least one of the previous years (2012 and 2013). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 52 October 2015 5.2.4.2.3. Roaming Behavior in the Middle River Several fish tagged at the Curry fishwheels moved downstream into a Lower or Middle River tributary (Table 5.2-2). For Pink Salmon tagged at the Middle River fishwheels, 39 percent of the classified fish exhibited this roaming behavior. Most of the roamers eventually went to either to Talkeetna River, Whiskers Creek or Chulitna River, although one fish moved far enough downstream to enter the Yenta River. Most Middle River Pink Salmon roamed as far upstream as the mouth of Indian River, others reached the mouth of Portage Creek, before turning downstream. 5.2.5. Sockeye Salmon 5.2.5.1. Fish Tagged in Middle River 5.2.5.1.1. Tag Returns Of the Sockeye Salmon radio tags deployed in the Middle River, five were recovered (Table E-2) in the Middle Susitna River (PRM 141 and 145; 2); as well as in Chase (1), Disappointment (1), and Larson (1) creeks. 5.2.5.1.2. Stock Classifications and Spawning Locations Of the 200 Sockeye Salmon tagged in the Middle River, 142 (71 percent) were classified by destination (Table 5.2-1). Of the remaining 58 tags, 9 were ignored (never moved from release site, or moved only in the downstream direction) and 49 exhibited movements that prevented conclusive assignment to the mainstem or tributaries (Table 5.2-1). Of the 142 Sockeye Salmon tracked to a spawning destination, 66 (46 percent) went to destinations in the mainstem Middle River (Table 5.2-1; Table D-6; Figure 5.2-1), all in slough/side channel habitats. The other 76 fish (54 percent) went to tributaries. Of these, 82.2 percent used Lower River tributaries (mainly Talkeetna or Chulitna rivers; Figure 5.2-3), and 11.8 percent used tributaries in the Middle River downstream from Devils Canyon (mainly Indian River or Portage Creek; Figure 5.2-3). Sockeye Salmon were tracked to eight potential mainstem spawning and holding sites in the Middle River between PRM 111.3 and PRM 155.9 (Table D-6). At seven of the eight sites, more than one radio-tagged Sockeye Salmon was detected (#2–8). No potential mainstem spawning sites for Sockeye Salmon were identified above PRM 146. To assess if there was spawning activity, all eight sites were visually examined during aerial telemetry, boat, or foot surveys (HSC). Four sites were surveyed aerially (#1, 2, 6, 7) and seven sites by boat/foot (#2– 8). No fish were observed at three of the sites (#1, 3, 4). Holding and spawning behavior was observed at five sites (#2, 5–8). All confirmed spawning sites were also confirmed in 2012 and 2013. 5.2.5.1.3. Roaming Behavior in the Middle River Sockeye Salmon tagged at the Curry fishwheels were the most likely species to roam (Table 5.2-2): 49 percent of the classified Sockeye Salmon did so. These Sockeye Salmon moved far up STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 53 October 2015 into the Middle River before dropping back to enter downstream tributaries. For example, three Sockeye Salmon moved upstream to Impediment 1 (two above and one below) before dropping back. Middle River Sockeye Salmon moved downstream to a variety of Lower River locations. Most of the roamers eventually went to either the Chulitna or Talkeetna rivers, but one moved far enough downstream to enter the Deshka River. 5.3. Objective 3: Characterize adult salmon migration behavior and timing within and above Devils Canyon 5.3.1. Chinook Salmon From 2012 to 2014, 17 large Chinook Salmon passed three impediments into the area above Devils Canyon (12 fish in 2012, 3 in 2013, and 2 in 2014; Table F-1). Of these, seven had final destinations that were above the proposed dam site, all in the Kosina Creek watershed (6 in 2012, 1 in 2014). A further three fish had destinations that were upstream of Devils Canyon but downstream of Watana (2 in Devil Creek and 1 in Tsusena Creek). Detailed results related to adult salmon migration behavior and timing within and above Devils Canyon in 2014 are presented below (see Tables 5.3-1 and 5.3-2) and in Appendices F and G. Of the 590 radio-tagged large Chinook Salmon tagged and released at the Middle River fishwheels in 2014, 491 were detected at or above Gateway Station (PRM 130.1) after tagging (Table 5.3-1). Of these 491 fish, 11 (2.2 percent) were tracked above Impediment 1 (PRM 155.2), 8 (1.6 percent) above Impediment 2 (PRM 160.2), and 2 (0.4 percent) above Impediment 3 (PRM 164.8). Thirty-four of the 656 large Chinook Salmon radio-tagged and released at the Lower River fishwheels were detected above Gateway Station, of which two were tracked above Impediment 1; one of which subsequently passed Impediment 2 (Tables 5.3-1 and 5.3-2; Figure 5.3-1). In addition, 32 radio-tagged small Chinook Salmon were tagged and released at the Middle River fishwheels. Of these, 24 were detected above Gateway Station after tagging and none passed Impediment 1 (Table 5.3-2; Figure 5.3-2). 5.3.1.1. Size of Chinook Salmon Tracked In and Above Devils Canyon In 2014, 38 radio-tagged Chinook Salmon passed and/or approached to within 1 km (0.6 mi) downstream of Impediment 1 (6 tagged in Lower River, 32 tagged in Middle River). The mean length of these fish (78.9 cm [31.1 in]) was significantly larger than that of radio-tagged fish which did not approach Impediment 1 (71.9 cm [28.3 in]; t522 = 3.10, P = 0.002). However, there was not a significant difference in length between fish that passed Impediment 1 (77.3 cm [30.4 in]) versus those that approached to within 1 km (0.6 mi) downstream of the Impediment (79.7 cm [31.4 in]); Table 5.3-1; t36 = 0.53, P = 0.60). The mean length of fish that approached, but did not pass Impediment 3 (79.0 cm [31.1 in], n = 4) was similar to that of fish that passed Impediment 3 (n = 2; Table 5.3-1). These observations suggest that length may have been a factor in approaching to within 1 km (0.6 mi) downstream of Impediment 1 for Chinook Salmon, but was not a factor in successful passing of the impediments (although sample sizes for statistical assessment of Impediment 3 passage were small). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 54 October 2015 5.3.1.2. Behavior of Chinook Salmon At Devils Canyon Fish showed noticeable milling or holding behavior below Impediment 1 and Impediment 3. Fish that moved past Impediment 1 held below it for an average of 3.9 days; similar in duration to individuals that did not pass (average 4.5 days; Table D-7). Four fish that passed Impediment 1 did not attempt to pass Impediment 2; rather they moved into Cheechako Creek, backed downstream to Portage Creek, or dropped downstream and died. All of the fish that approached Impediment 2 passed it quickly (≤ 1 day; Table D-7). Three fish that passed Impediment 2 did not attempt to pass Impediment 3; rather, they explored the area around Chinook Creek, and eventually dropped back downstream. When considering patterns for the six fish that approached Impediment 3, the hold times were shorter and approach dates were later for the fish that passed as compared with those that did not pass. The two fish that passed Impediment 3 held below it for an average of 6.8 days, whereas those that did not pass, held for an average of 11.3 days before moving downstream (Figure 5.3-3). The two fish that passed approached on or after July 30, where approach dates of the non-passing fish ranged from July 2 to 28. The destination for the 13 Chinook Salmon tracked above Impediment 1 are provided in Table 5.3-3. Three of four Chinook Salmon that did not pass Impediment 2 could be conclusively assigned to a spawning destination, with one (33 percent) of those being downstream of Impediment 1. Three of seven Chinook Salmon that did not pass Impediment 3 could be conclusively assigned to a spawning destination, and all 3 (100 percent) had a destination downstream of Impediment 2. Overall, seven of the 13 Chinook Salmon that passed at least one of the three impediments could be conclusively assigned to a spawning destination; and 4 of 7 destinations (57 percent) were downstream of the last impediment passed – two of these went to Portage Creek, one to Cheechako Creek, and one to the mainstem near the mouth of Cheechako Creek. 5.3.1.3. Passage Timing and Flows In 2014, the first Chinook Salmon to successfully pass Impediment 1 passed on June 30 when flow at the Tsusena Creek gauge was 19,400 cfs (26,000 cfs at the Gold Creek gauge; Figure 5.3-1). Other fish passed Impediment 1 on July 1 and July 6, during flows of 23,200 cfs or greater at the Tsusena Creek gauge (27,900 cfs or greater at the Gold Creek gauge). No other fish passed Impediment 1 until the period from July 18 to August 1, when Tsusena Creek gauge flows ranged between 15,500 and 23,400 cfs (18,800–27,100 cfs at the Gold Creek gauge). There was a period from July 7 to July 17 during which no fish passed, and flows ranged from 19,900 to 35,300 cfs at the Tsusena Creek gauge (24,200–36,500 at the Gold Creek gauge; Table 5.3-1; Figure 5.3-1). Both Chinook Salmon that later passed Impediment 3, had passed Impediment 1 on the same day (July 20; Table D-7). Discharge when the two fish passed Impediment 3 ranged from 15,900 cfs (July 30) to 16,400 cfs (August 4) at the Tsusena Creek gauge (19,200–19,400 cfs at the Gold Creek gauge). 5.3.1.4. Behavior Upstream of Devils Canyon Two Chinook Salmon passed Impediment 3, each showing markedly different behaviors (Figures F-1 and F-2). One Chinook Salmon just barely passed Impediment 3, subsequently returned STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 55 October 2015 downstream of it, and eventually died in the mainstem downstream of Devils Canyon (Figure F- 1). The other Chinook Salmon travelled directly into Kosina Creek, spent 6 days therein, then took 5 days to swim to and return from Oshetna River (40 km [25 mi] each way), before returning to Kosina Creek (Figure F-2). This latter fish stayed in Kosina Creek for another 6 days, and then drifted out, settling just downstream of the mouth of Fog Creek. 5.3.1.5. Programmatic Summary There was not strong or consistent evidence to suggest that body length influenced passage success through Devils Canyon. In 2012, Chinook Salmon that passed Impediments 1 and 3 were larger on average than those than dropped back, but the differences were not statistically significant (AEA 2013a). In 2013 and 2014, Chinook Salmon that passed the impediments were slightly smaller on average than those that dropped back, and again the differences were not statistically significant (AEA 2014c). In 2014, the Chinook Salmon that approached Devils Canyon were significantly larger on average than the fish that never came within 1 km of Impediment 1, but in 2012 and 2013 the same pattern was not observed. For large Chinook Salmon tagged at the Middle River fishwheels in 2012 and 2014, the mean length of fish that passed Impediment 3 was longer than that of the rest of the population (samples sizes were too small for rigorous statistical analysis), yet the pattern was opposite in 2013 (unpublished data). Overall, Chinook Salmon that ascended the impediments were at least 61 cm METF, but Sockeye Salmon that passed Impediment 1 ranged from 45–49 cm METF. Given the inconsistent results, it remains uncertain whether length is an important factor in successful passage above the impediments. In 2012, there were results to suggest that river flow rates influenced passage success through Devils Canyon (AEA 2013a). In 2013, results similarly suggested that Impediment 3 passage occurred only when Susitna River flows were lower than 19,000 cfs near Tsusena Creek (20,000 cfs at the Gold Creek gauge). In 2014, fish passage at Impediment 3 occurred only during periods in which flow at the Tsusena Creek gauge dropped below 16,500 cfs (19,500 cfs at the Gold Creek gauge). In all years, results were limited in sample size, and were not part of a formal control-treatment experiment. Therefore, the degree to which lower flows played a primary causal role in the passage of Chinook Salmon is not quantifiable. Of particular concern is the potential confounding effects of “time of year.” Since, in all years, fish moved above the final impediment during lower-flow periods that occurred at around the same timeframe (July 17–20 in 2012; July 13–30 in 2013; July 30 to August 4 in 2014), it is not possible to separate out the relative importance of flows versus time of year. Adult salmon migration behavior and timing within and above Devils Canyon has several consistent characteristics. As we currently understand it, only large Chinook Salmon (METF ≥ 50 cm [19.7 in]) regularly move upstream of Devils Canyon. Individuals that move upstream of the canyon are not significantly different in size than other large Chinook Salmon. Passage generally occurs in mid or late July. Passage events have not been observed during the highest flow conditions (Table 5.3-1, Figure 5.3-1), yet specific flows that may trigger canyon- passage behaviors were not evident from the data, other than that many occurred near the 10% exceedance level. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 56 October 2015 The main spawning destination in the Upper River is Kosina Creek, where peak counts have ranged from 26 July to 5 August (Table 5.3-4; Figure 5.3-4; i.e., more than 2 weeks after the radio-tagged fish were observed passing Impediment 3). Middle River spawning destinations that are upstream of Devils Canyon and downstream of the dam site include Tsusena, Fog and Devil creeks, where peak counts have ranged from 21 July (in Fog Creek in 1984) to 15 August (in Fog Creek in 2013; Table 5.3-5; i.e., 2-4 weeks after radio-tagged fish were observed passing Impediment 3). Within the canyon, Chinook Salmon spawn in Cheechako and Chinook creeks, where peaks counts have generally been in late July or early August, although the peak count in 1985 was quite late (23 August in Chinook Creek; Table 5.3-5). Small Chinook (METF < 50 cm [19.7 in]) and Sockeye salmon have successfully passed Impediment 1, but have always returned downstream shortly thereafter without passing Impediment 2. 5.3.1.6. Aerial Spawner Surveys Chinook Salmon was the only salmon species observed during aerial spawner surveys that were flown from Cheechako Creek upstream to the Oshetna River in 2014. Adult Chinook Salmon were observed in Middle River tributaries between Impediments 1 and 2 (Cheechako Creek), between Impediments 2 and 3 (Chinook Creek), and above Impediment 3 (Devil and Fog creeks). No adult salmon were observed in the mainstem Susitna River or in any Upper River tributaries in 2014 (Table D-8). In streams where they were observed, adult Chinook Salmon were not consistently seen over the course of the season. They were documented in one stream during Survey 1, two streams during Surveys 2 and 3, four streams during Surveys 4 and 5, one stream during Survey 6, and two streams during Survey 7 (Table D-8). Peak observations occurred on July 19 in Cheechako and Chinook creeks, July 31 in Fog Creek, and August 6 in Devil Creek. In Cheechako Creek, the number of Chinook Salmon observed increased from 11 fish on July 14 to 16 fish on July 19. A steady decline in fish numbers was observed following July 31, and by the final survey on August 18, zero fish were seen. Chinook Creek followed a similar trend with five fish observed during the July 19 and July 25 surveys, two fish on the July 31 and August 6 surveys, and zero fish observed on the last two surveys. Cheechako Creek is located between Impediment 1 and Impediment 2. It has high-gradient, step-pools within steep-walled canyons. Chinook Salmon habitat terminates within three miles of the Susitna River confluence at large waterfalls. The turbulent water and confined canyon walls presented challenges to salmon observation. Nevertheless, salmon were observed throughout this lower 3-mile reach of this creek, and groups of fish were seen holding in pools. In Cheechako Creek, a no-fly zone around a raptor nest within the canyon prevented aerial observation of approximately 0.5 miles of stream during the first two surveys. Chinook Creek is located between Impediment 2 and Impediment 3. Chinook Creek has long stretches of high-gradient cascades, but it does not have a barrier to Chinook Salmon migration below 3,000 ft in elevation where the surveys terminated (at approximately tributary river mile 9). Regardless, fish were not seen beyond 0.5 miles upstream of the Chinook Creek confluence with the Susitna River. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 57 October 2015 Devil Creek is located just upstream of Devils Canyon. Devil Creek has similar geomorphology to Cheechako Creek, featuring high-gradient, step-pools within steep-walled canyons. Chinook Salmon habitat terminates within three miles of the Susitna River confluence at large impassable waterfalls. The turbulent water and confined canyon walls presented challenges to salmon observation. Nevertheless, fish were observed throughout the anadromous reach of this creek, and groups of fish were seen holding in pools. Low numbers of adult Chinook Salmon were observed in Fog Creek. The lower reach of Fog Creek, where all the Chinook Salmon were observed, is dominated by high-gradient riffle confined within a steep-walled canyon. The upper reach is much lower gradient and has long stretches of gravel and cobble substrate that appeared suitable for Chinook Salmon spawning. An additional five miles of a tributary to Fog Creek was also surveyed. Three adult Chinook Salmon were observed in Fog Creek during Survey 4 on July 31; two fish were seen on Survey 5; zero on survey 6; and then one fish was observed during the final survey on August 18. The farthest upstream observation was approximately three miles from the Fog Creek confluence with the Susitna River. Overall, weather conditions were favorable for observation throughout the study duration. While weather was variable, it was not a limiting factor in observing fish and did not delay survey completion (Table D-9). Low clouds and rain were the most influential weather factors; low light conditions occasionally reduced visibility into the deeper water and rain on the aircraft windows sometimes created difficult viewing conditions. Sun glare on the water was not a major factor in limiting fish observations; polarized glasses, helicopter orientation, and survey direction (some streams were surveyed from upstream to downstream) worked to improve visibility when glare was present. Tall trees and overhanging vegetation partially obscured some areas of most streams (Table D-10). Overall, most streams had very clear water. The Black and Oshetna rivers were the only streams of glacial origin within the study area and turbidity severely limited visibility in the Black River and the Oshetna River downstream of the Black River confluence during all surveys. Visibility in Watana and Jay creeks was typically poor in the lower few miles due to erosion produced from landslides; whereas upstream of the landslides the water was clear. Kosina Creek experienced several minor erosion events upstream of the study area which reduced visibility slightly. The most prevalent impairment to seeing fish was white-water turbulence, which was significant within all streams surveyed; white water was noted as limiting the observer’s ability to find fish during all surveys (Table D-10). 5.3.1.7. Using Sonar to Enumerate Salmon at the Proposed Dam Site Detailed results pertaining to 2014 field activities are provided in Appendix G, and a brief summary is provided here. During sonar operations from July 6 to August 22 at the proposed dam site, a total net-upstream count of 24 Chinook Salmon was estimated based on the number of fish measuring 50 cm (19.7 in) TL or greater. Passage of these fish at Watana was observed from July 10 through August 22, and compared well with the passage of radio-tags at Impediment 3 on July 20. Aerial spawner surveys were conducted between July 14 and August 19, so multiple forms of STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 58 October 2015 monitoring were occurring in the region over the same time period. The sonar array covered approximately 58 percent of the channel width where the substrate was in the field of view and water velocities outside the sonar range were too high (greater than 1.8 m/s [6 ft/s]) to be a primary migration corridor. All observations of fish were within 4 m (13.1 ft) of the transducers with most occurring at 3 m (9.8 ft). No apparent diel passage patterns were observed. Size estimates for upstream migrants ranged from 50 to 110 cm (19.7–43.3 in) TL, with an average of 78 cm (30.7 in) TL. In addition, 213 fish measuring 40–49 cm (15.7-19.3 in) TL, and 1,044 fish measuring less than 40 cm (15.7 in) TL, were counted at the sonar stations. Assuming the length distribution of Chinook Salmon at Watana was the same as that at the Middle River fishwheels, then an estimated 24.1 percent of Chinook Salmon at Watana were less than 50 cm (19.7 in). This percentage would be a conservative estimate based on the length analysis presented in Section 5.3.1.1 that demonstrated bigger fish were the ones approaching the impediments in Devils Canyon. Even so, applying this conservative estimate to the sonar data, 24 Chinook Salmon at Watana (targets ≥ 50 cm [19.7 in]) then represent 75.9 percent of the Chinook Salmon that passed, and 8 additional Chinook Salmon measuring less than 50 cm (19.7 in) could have passed (= 24 * 24.1 / 75.9). Note however, based on radio telemetry, no tagged Chinook Salmon measuring less than 50 cm (19.7 in) METF have passed Impediment 3 in three seasons of study. Considering all available information, it was estimated that greater than 90 percent of Chinook Salmon migrating past Watana were observed by the sonar, or that about an additional 3 passage events could have occurred. 5.3.2. Sockeye Salmon Of the 200 radio-tagged Sockeye Salmon released at the Middle River fishwheels, 146 were detected at or above Gateway Station (PRM 130.1; Table 5.3-1). Of these 146 fish, 3 (2.1 percent) were tracked above Impediment 1 (Tables 5.3-1 and 5.3-2; Figure 5.3-2). From 2012 – 2014, a total of 409 Sockeye Salmon were radio-tagged in the Middle River, and of these, 3 were tracked above Impediment 1 (i.e., no Sockeye Salmon passed Impediment 1 in 2012 or 2013). 5.3.2.1. Size of Sockeye Salmon Tracked At Devils Canyon Sixteen radio-tagged Sockeye Salmon passed and/or approached to within 1 km (0.6 mi) downstream of Impediment 1. The mean length of these fish (48.5 cm [19.1 in]) was not significantly different from that of fish that did not approach Impediment 1 (50.8 cm [20.0 in]; t144 = 1.7, P = 0.09). The mean body length of fish that approached, but did not pass Impediment 1 (48.8 cm [19.2 in]) was not significantly different from that of fish that passed Impediment 1 (47.3 cm [18.6 in]; Table 5.3-1; t14 = 0.44, P = 0.66). These observations indicate length was not a factor in determining approach or successful passage of Impediment 1 for Sockeye Salmon (although sample sizes for statistical assessment of impediment passage were small). 5.3.2.2. Behavior of Sockeye Salmon At Devils Canyon The first Sockeye Salmon to successfully pass Impediment 1 passed on July 27 (Table D-7) when flow at the Tsusena Creek gauge was 18,900 cfs (Figure 5.3-2; 23,900 cfs at the Gold Creek gauge). No other Sockeye Salmon passed Impediment 1 until September 8, when two fish were detected just above Impediment 1, but which returned downstream by the next day. From July 27 to September 10, flows were relatively stable, averaging 15,600 cfs and ranging from STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 59 October 2015 12,900 to 18,900 cfs at the Tsusena Creek gauge (Figure 5.3-2; at Gold Creek gauge, flows averaged 18,972, and ranged from 16,200 to 23,900 cfs). Sockeye Salmon that moved past Impediment 1 held downstream for an average of 1.5 days before passing upstream; this is a shorter holding time than for individuals that did not pass (average 4.7 days; Table D-7). None of the three Sockeye Salmon that passed Impediment 1 approached Impediment 2. All three of the Sockeye that passed Impediment 1 were later tracked to spawning destinations that were downstream of the impediment: two went to the Chulitna River, and one to Jack Long Creek. 5.3.2.3. Aerial Spawner Surveys No adult Sockeye Salmon, nor Chinook Salmon, were observed during 2014 aerial surveys of the two lakes in the Tsisi Creek drainage. Surveys were conducted approximately two weeks after the timing of documented salmon passage above the Devils Canyon impediments and thus were consistent with the timing of salmon presence in the Upper River. In addition, opportunistic fish sampling was conducted in one of these lakes in late August and September and demonstrated the presence of Lake Trout in the size range of 372-456 mm fork length (R2 Resource Consultants 2015). 5.3.3. Other Species No salmon species other than Chinook and Sockeye salmon were detected within Devils Canyon in any of the three recent years of telemetry study and only Chinook Salmon were documented above Impediment 3 of the canyon (AEA 2013a, 2014c). 5.4. Objective 4: Use available technology to document salmon spawning locations in turbid water Radio telemetry surveys were used to locate potential spawning locations for Chinook Salmon in turbid water habitats of the Middle River from 2012–2014. In total, radio telemetry identified 98 radio-tagged Chinook Salmon suspected of spawning within turbid mainstem habitats of the Middle River. The locations of these Chinook Salmon led ground crews to sample 44 potential spawning areas, of which 18 were sampled using sonar technologies (side-scan, DIDSON, and ARIS). Seven of the 44 potential spawning locations were repeat locations in at least two of the three sampling years (2012-2014). Of the 18 locations sampled with sonar over the three study years (sampling dates ranged from July 19 to July 31), Chinook Salmon spawning activity (i.e., nest-guarding behavior) was observed at one location in the mainstem Susitna River: near the mouth of the Indian River (Table 5.4-1). At four turbid water locations (4th of July Creek Mouth, Portage Creek Mouth, and two main channel habitats), Chinook Salmon were present or holding, but exhibited no behavior indicative of spawning. The remaining 26 locations were either not accessible by boat, were not suitable as spawning habitat, or did not contain Chinook Salmon when viewed with sonar. Additionally, Chinook Salmon spawning behavior was confirmed using sonar at the mouth of Jack Long Creek (2014); however, this location was not identified by radio telemetry analysis. During the latter part of the Chinook Salmon migration in early August, the presence of Chum Salmon at some locations made confirmation of Chinook Salmon difficult. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 60 October 2015 Spawning activity was not confirmed for any other salmon species using sonar technology. The utility of using sonar to document spawning in turbid water was limited for several reasons. First, Chum, Coho, Pink, and Sockeye salmon have a complete overlap in body size and run timing making confirmation of species in turbid water using sonar impossible. Finally, due to bathymetry and substrate size at many locations sonar imagery was not effective for detecting features indicative of redds. Similarly, due to the slope of the river bed, redd digging behavior could not be captured despite collecting several hours of imagery containing Chinook Salmon and other salmon species in areas considered suitable for spawning. Worthy of mention, sonar was able to confirm spawning behavior for a species of salmon at 4th of July Side Channel; however, species ID was not able to be confirmed until water clarity increased later in the season allowing visual confirmation of species. 5.4.1. Programmatic Summary Over the three-year period, several limitations of the sonar method were identified. Redd depressions were often difficult to observe in sonar imagery because of the slope of the river bed or size of the substrate relative to the orientation of the redd depression. Tiffan et al. (2004) found that DIDSON was more effective when Chinook Salmon redds exhibited sufficient morphology (i.e., well-developed tailspills) and where the topography of the river bed was somewhat smooth so that redds would not be confused with other bottom features. When a fish swam into a redd depression (e.g., to dig), it could not be detected by the sonar. Given proper orientation, fish could be observed on the sonar holding, swimming in and out of areas considered to be redds, and displaying nest-guarding behavior. However, when a fish was not oriented perpendicular to the sonar beam its acoustic signal became weak which made identifying behavioral observations difficult. At times when multiple fish species with a large degree of overlap in body length could be present (e.g., Chinook/Chum or Chum/Coho/Pink/Sockeye), sonar was not able to positively identify species. This was less of an issue for Chinook Salmon which spawned in part during a period when few other species were present, and because they are typically much larger than most other salmon species. And lastly, the equipment needed to operate a sonar was heavy and required a boat with a suitable gunnel- mount. Many of the potential spawning locations were in side channels or sloughs that were too shallow for the boat, so this limited the number of sites that could be sampled using the equipment. Still, the study team successfully met this objective by demonstrating that sonar technologies could be used to document spawning behaviors, but that the utility was limited, given the current state of the technology, by bed topography and shallow depths at which salmon spawn. 5.5. Objective 6: Generate counts of adult Chinook Salmon spawning in the Susitna River and its tributaries In July 2012, stream walks (visual counts) and aerial-tracking surveys proved to be ineffective for generating counts and establishing mark rates in sections of the Indian River and Portage Creek due to bear predation on Chinook Salmon. Thus, no escapement estimates for Chinook Salmon above Devils Canyon were made in 2012. In 2013, a weir and underwater video system were used to estimate an escapement of 1,137 large Chinook Salmon (mark rate of 6.3 percent) to the Indian River. These data were used to estimate escapements of 6,952 large Chinook Salmon above Gateway, and 48 large Chinook Salmon migrated above Devils Canyon in 2013. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 61 October 2015 In 2014, the Indian River weir was rendered inoperable due to a flood so the study team used the AUC method to estimate an escapement of 1,297 large Chinook Salmon in the Indian River (mark rate of 13.2 percent). Detailed results related to counts of adult Chinook Salmon spawning in the Susitna River and its tributaries in 2014 are presented below and in Appendix H. 5.5.1. Indian River Escapement Estimate Chinook Salmon abundance in the Indian River was estimated using the AUC method (Appendix H). From July 7 to August 19, 16 aerial spawner surveys were conducted in the Indian River (Table 4.3-1). In all three river sections combined, 127 Chinook Salmon were counted on the first survey (July 7) and three fish were counted on the last survey (August 19; Table H-1). Observed counts peaked at 798 Chinook Salmon on July 22. Estimated observer efficiencies ranged from 40 to 80 percent (Table H-1). The lowest observer efficiencies occurred on July 19 due to poor weather conditions. For most surveys, conditions ranged from good to excellent. Chinook Salmon counts in the Indian River were very similar during concurrent surveys conducted by the study team and ADF&G. ADF&G counted 558 Chinook Salmon on July 31 (S. Ivey, ADF&G, Sport Fish Division, personal communication) and the study team observers counted 544 Chinook Salmon on August 1. For the purpose of analysis, it was assumed that no Chinook Salmon were present in the Indian River prior to June 26 or after August 19 (Figure H-1). No Chinook Salmon were observed at the Indian River weir through June 25, and survey crews indicated that the three remaining live Chinook Salmon observed on August 19 were moribund. Residence times above Bridge 1 for radio-tagged large Chinook Salmon averaged 15.0 days and ranged from 0.1 to 60.6 days (median = 15.6 days, n = 184; Figure H-2). Based on the observed aerial survey counts, estimated observer efficiencies, and median residence time of radio -tagged fish, the estimated escapement of large Chinook Salmon above Bridge 1 in 2014 was 1,297 fish (Table H-2). Over the study period, 184 radio-tagged large Chinook Salmon were detected above Bridge 1, of which 171 were tagged in the Middle River and 13 were tagged in the Lower River. Thus, the mark rate of large Chinook Salmon at the Middle River fishwheels was estimated to be 13.2 percent (171 tags/1,297 fish; Table H-2). In 2013, estimated observer efficiencies for Chinook Salmon in the Indian River during the study team and ADF&G aerial spawner surveys ranged from 35.8 to 46.3 percent (Table H-1 in AEA 2014c). Based on the 2013 results, it is possible the 2014 estimates of observer efficiency were biased high. For this reason the study team conducted a sensitivity analysis of the 2014 escapement estimate and mark rate to determine how sensitive calculations were to a small decrease in observer efficiency. All else remaining constant, decreasing observer efficiencies by 15 percent for each survey and river reach led to a 29 percent increase in the escapement estimate (from 1,297 to 1,674 fish) and a 23 percent decrease in the mark rate (from 13.2 to 10.2 percent). 5.5.1.1. Programmatic Summary In the Middle River, efforts to count Chinook Salmon in tributaries from 2012 to 2014 were focused on Indian River (with some minor effort in Portage Creek in 2012) for the purposes of STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 62 October 2015 estimating survey-area mark rates (to test the representativeness of tagging efforts), estimating abundance above the tagging site, and making inferences about the relative abundance among recovery locations. Visual counts (stream walks) in the Indian River and Portage Creek in 2012 proved difficult due to the removal of Chinook Salmon by bears. In 2013, 1,137 large Chinook Salmon were inspected for tags at the Indian River weir, of which 72 (6.3 percent) were radio- tagged. Based on the number of fish entering the study area, mark -recapture methods were used to estimate that 6,952 (SE = 682) large Chinook Salmon passed the Gateway Station in the Middle River from June 18 to July 28 in 2013. In 2014, since the Indian River weir was rendered inoperable, AUC methods were used to generate an escapement estimate of 1,297 large Chinook Salmon, of which 13.2 percent were tagged. 5.5.2. Estimated Abundance of Chinook Salmon Upstream of Devils Canyon Abundance of Chinook Salmon upstream of Devils Canyon was estimated using two methods. The first method expanded the counts of radio-tagged fish by the ‘mark rate’ (i.e., the proportion of fish that were marked). In 2014, the mark rate was 13.2 percent: approximately one in every 7.6 large Chinook Salmon at the Middle River fishwheels was tagged (Table H-2). For this method, it is important to understand that the positions of the fixed-station receivers and the extensive mobile survey effort made it unlikely that any radio-tagged fish passed upstream of Devils Canyon undetected. Since, in 2014, two radio-tagged large Chinook Salmon passed Impediment 3, and one tagged fish passed the Watana Dam site (Tables 5.3-1 and 5.3-2), the study team would expect that 15.2 large Chinook Salmon (2 divided by 13.2 percent) passed Impediment 3, and 7.6 fish (1 divided by 13.2 percent) passed Watana Dam site. In 2014, a second independent method was available to estimate the number of salmon that passed the dam site. Specifically, a sonar was deployed at the Watana site, and a total of 24 salmon-sized targets passed during the study period. Given the species composition of the radio- tagged fish that passed Impediment 3, coupled with that from spawner surveys, it is probable that all of these targets were Chinook Salmon. Together, these two methods suggest that the abundance of Chinook Salmon upstream of the dam site likely ranged from 7.6 to 24 fish. 5.5.2.1. Programmatic Summary The abundance of Chinook Salmon passing Devils Canyon was not directly estimated in 2013 or 2014 (e.g., using mark-recapture methods). However, mark rates established for large Chinook Salmon in the Indian River, combined with the number of radio-tagged fish present above Devils Canyon, were used to make inferences about the relative abundance of large Chinook Salmon above Devils Canyon. In 2013, an estimated 48 large Chinook Salmon migrated above Devils Canyon (3 radio tags present above Devils Canyon expanded by 6.3 percent mark rate). Although too few radio-tagged fish migrated above Devils Canyon to develop a statistically precise estimate, an estimate of similar magnitude was produced when the peak aerial spawner count was expanded by the estimated observer efficiency (29 fish counted expanded by 46.3 percent observer efficiency = 63 fish total). In 2014, results from three independent study components indicated that the abundance of large Chinook Salmon above Devils Canyon was likely on the order of magnitude of 50 fish or less, with a portion of that number passing above the potential dam site. First, aerial spawner surveys STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 63 October 2015 of clear-water tributaries above Devils Canyon had a peak count of 12 Chinook Salmon (10 in Devil Creek, 2 in Fog Creek). If the observer efficiencies on these surveys were as low as 40–50 percent, the peak count would expand to only 24–30 fish. Second, a net-upstream count of 24 salmon-sized fish was obtained at the Watana Canyon sonar site in 2014. Although this count was considered a minimum estimate, there was no evidence to suggest that fish passage at the site was significantly greater than that observed. And third, based on a 13.2 percent mark rate for large Chinook Salmon at the Middle River tag site, the two radio-tagged fish that migrated above Devils Canyon represented approximately 15 fish in total. 5.6. Objective 7: Collect tissue samples to support the Fish Genetics Study Genetic samples were collected from 4,016 Chinook, 395 Chum, 2,074 Coho, 798 Pink, and 336 Sockeye salmon across the three study years and sample locations. In 2012, 443 Chinook Salmon were sampled. In 2013, genetic samples were collected from 1,999 Chinook, 201 Chum, 1,016 Coho, 399 Pink, and 138 Sockeye salmon. In 2014, genetic samples were collected from 659 Chinook, 640 Coho, and 198 Pink salmon in the Lower River. Genetic samples were also collected from 296 Chinook Salmon in the Yentna River and 204 Coho Salmon in Montana Creek. In the Middle River, genetic samples were collected from 619 Chinook (small and large fish combined), 194 Chum, 214 Coho (199 at fishwheels, 15 in gillnets), 201 Pink, and 198 Sockeye salmon in 2014 (Table A-6). All genetic tissue samples were delivered to ADF&G’s Gene Conservation Lab for analysis. Results were reported by the Genetic Baseline Study for Selected Fish Species (Study 9.14). The study team completed all tasks required to fulfill the requirements of study Objective 7. Genetic samples were collected from adult anadromous salmon in conjunction with addressing study objectives 1 and 2. All samples were delivered to ADF&G’s Gene Conser vation Lab for analysis, and results were reported by the Genetic Baseline Study for Selected Fish Species (Study 9.14). 5.7. Objective 8: Estimate the system-wide Chinook Salmon escapement and the Coho Salmon escapement to the Susitna River above the Yentna River, and the distribution of those fish among tributaries of the Susitna River In 2013, the abundance of Chinook Salmon measuring 50 cm (19.7 in) METF or greater spawning in the Susitna River above the Lower River mainstem tagging site was 89,463 (SE = 9,523). Based on radio-telemetry tracking results (see Table 5.2-1 in the ISR), it is estimated that Deshka, Talkeetna and Chulitna Rivers were the main destinations (Figure 5.7-1). As discussed in Study 9.7 ISR Appendix I, no estimate was produced for the Yentna River in 2013. In 2014, the system-wide escapement estimate for Chinook Salmon was 68,225 [SE = 10,615] upstream of the confluence of the Yentna River and 22,267 [SE = 2,871] in the Yentna River). Based on radio-telemetry tracking results (Table 5.2-1), it is estimated that Deshka, Talkeetna and Chulitna Rivers were the main destinations (Figure 5.7-2) upstream of Yentna River. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 64 October 2015 The 2014 escapement estimate (84,879; SE = 9,550) for Coho Salmon measuring 40 cm (15.7 in) METF or greater above the Lower River tagging site was 35 percent lower than the 2013 estimate (130,026; SE = 24,342). Based on radio-telemetry tracking results (Table 5.2-1), it is estimated that Deshka, Talkeetna and Chulitna Rivers were the main destinations in both years (Figures 5.7-3 and 5.7-4). Detailed results related to the 2014 escapement and distribution estimates as part of Objective 8 are provided in Appendix I. 6. DISCUSSION This Discussion section focuses on Objective 5 of the RSP: “Compare historical and current data on run timing, distribution, relative abundance, and specific locations of spawning and holding salmon.” Recent and historic data pertinent to these aspects of salmon ecology are reviewed by species for the Lower and Middle Susitna River. Fish abundance as calculated by mark-recapture experiments (i.e., escapement estimates) were determined in the early 1980’s (1981-1984), and recently as part of the Susitna-Watana project. Marking target species was accomplished with visual tags or radio-tags via capture by fishwheels. A portion of the tags were then recovered at weirs or by telemetry monitoring at strategic locations in the basin. Historically, escapement estimates were derived for the Lower Susitna (Sunshine Station), the Middle Susitna (Talkeetna Station), and select tributaries. More recent (2000’s) Lower River estimates are available for salmon species except Pink Salmon, and recent Middle River estimates are available for Chinook and Coho salmon. All studies partition out abundance of the Yentna River so that general comparisons can be made between the historical and recent estimates upstream of its confluence. Estimating Chinook and Coho salmon abundance in 2013 and 2014 accomplishes Objective 8 of the Study Plan. Habitat classifications described during studies conducted in the 1980’s (Barrett et al. 1985a,b; Thompson et al. 1986) were not defined exactly as in the 2012-2014 studies, and should be taken into consideration when making comparisons. Specifically, spawning habitat studies conducted from 1981 to 1983 classified a “tributary mouth” as including the lower 1/3 mile of the tributary, and survey sections did not sample the zone of confluence with tributary and the Susitna River. However, in 1984, the methodological approach was modified, and spawning behavior at the confluence of the tributary was documented for several streams in the Middle River (Barrett et al. 1985a,b). For the purpose of analysis, only historic spawning locations that specifically mentioned the confluence with the Susitna River were used for comparison with recent spawning locations. 6.1. Chinook 6.1.1. Timing of Migration  Lower River Fishwheel catches were compared among years to assess variability in the timing of migration through the Lower River; however, it should be noted that some conditions varied across years STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 65 October 2015 (e.g., number of fishwheels operated, location of fishwheels, operational period, daily fishing effort, river discharge, relative fish abundance). From 2012 to 2014, fishwheels were operated in the Lower River (PRM 33–34) and Chinook Salmon were captured as early as May 22 and as late as August 19. Peak catches occurred in early to mid-June in 2012, 2013, and 2014. This run timing from recent studies is considerably earlier that than reported from studies in the 1980s. In 1982 and 1983, Chinook Salmon were captured from June 28 to September 2 at the Lower River fishwheels (ADF&G 1983b, 1984). At the Flathorn fishwheels (PRM 26–28), Chinook Salmon were caught between June 30 and August 27 in 1984 and 1985 (Barrett et al. 1985a,b; Thompson et al. 1986). And in 1983, Chinook Salmon were captured at the Sunshine fishwheels (~PRM 84) from June 5 to August 18 (ADF&G 1984).  Middle River (downstream of Devils Canyon) Fishwheels were operated in the Middle River near Curry from 1981 to 1985 and 2012 to 2014. Again, it should be noted that many factors related to fishwheel sampling were not constant across years. From 2012 to 2014, Chinook Salmon (all sizes combined) were captured at the Middle River fishwheels as early as June 11 and as late as August 28 (Table A-8, Figure A-13). This run timing was similar to historic records from the 1980s, when the earliest that a Chinook Salmon was captured at the Middle River fishwheels was June 9 (1984) and the latest was August 20 (1981; Table A-8, Figure A-13). The midpoint of Chinook Salmon catches ranged from June 30 to July 3 for the years 2012 to 2014, and from June 24 to July 9 for the years 1981 to 1985. The dates of peak catch were similar over the recent 3-year period (July 2 in 2012 and 2013, and July 1 in 2014).  Middle River (upstream of Devils Canyon), Watana Dam, Upper River Migration timing for radio-tagged Chinook Salmon passing Impediment 3 in the Middle River was fairly consistent in recent study years and ranged from July 17–20 in 2012 (n = 12), July 13– 30 in 2013 (n = 3), and July 30 to August 4 in 2014 (n = 2). This timing was similar to that of Chinook Salmon observed upstream of Impediment 3 during two years of aerial spawner surveys (2012 and 2013). Furthermore, at the proposed Watana Dam site, shore-based sonar units detected fish determined as Chinook Salmon, from July 10 to August 22 in 2014 (Appendix G) and on July 20, 2013. There was no run timing data upstream of Devils Canyon from the 1980s studies to compare to recent studies. 6.1.2. Timing of Spawning  Lower River and Tributaries The migration timing of Chinook Salmon in the Lower River was slightly earlier and more protracted for recent studies as compared to timing data from the 1980s. For Chinook Salmon classified to the Yentna, Deshka, Chulitna or Talkeetna rivers, the 10th and 90th percentile dates when fish were spawning ranged from June 24 to August 10. Each potential mainstem spawner had a single location position that was selected as representative of its ‘cluster’ (see Section 4.2.3.2), and each of those representative locations had an associated date. The 10th and 90th percentile dates for Chinook Salmon Lower River mainstem destinations ranged from June 22 to September 2. In comparison, from 1981 through 1984, the spawning period for Chinook Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 66 October 2015 in the Lower River ranged from mid-July through mid-August, with peak spawning occurring during the last week of July (summarized in Barrett et al. 1985a,b).  Middle River (within and downstream of Devils Canyon) and Tributaries For Chinook Salmon classified to Indian River or Portage Creek, the 10th and 90th percentile dates during which fish were in the tributary to which they were classified spanned from July 8 to August 6. The 10th and 90th percentile ‘representative’ dates for Chinook Salmon Middle River mainstem destinations downstream of Devils Canyon ranged from July 12 to August 3. In comparison, from 1981 through 1984, the spawning period for Chinook Salmon in the Middle River was very similar ranging from mid-July through mid-August, with peak spawning occurring during the last week of July (summarized in Barrett et al. 1985a,b). For Chinook Salmon classified to Devils Canyon tributaries (Cheechako or Chinook creeks), the 10th and 90th percentile dates during which fish were in the tributary to which they were classified spanned from July 17 to August 7. From 2012-2014, two Chinook Salmon were classified as having mainstem destinations within Devils Canyon. The ‘representative’ dates for the two fish were July 21 and September 2.  Middle River (upstream of Devils Canyon), Watana Dam, Upper River and Tributaries. For Middle River tributaries upstream of Devils Canyon (Devils or Tsusena creeks), the 10th and 90th percentile dates ranged from July 22 to August 9. In the Upper River (i.e., in Kosina Creek and its tributaries), date ranges were July 23 to August 11. Aerials surveys conducted in the 1980s documented few spawners in the areas upstream of Devils Canyon. In 1983, the single aerial survey over Devil Creek (August 1) documented the presence of one fish. In 1984, four flights over Fog Creek found a peak count of two fish on July 21. Other than these few data points, the historical timing of spawning for Chinook Salmon upstream of Devils Canyon was not previously documented. 6.1.3. Distribution to Mainstem and Tributaries 6.1.3.1. Radio-tags applied in Lower River  Lower River and Tributaries During 2012-2014, Chinook Salmon tagged in the Lower River moved mostly into Lower River tributaries. The main Lower River tributary destinations were the Yentna (13.2 percent), Deshka (24.5 percent), Talkeetna (16.2 percent) and Chulitna (16.6 percent) rivers (Figure D-1). In addition, 0.6 to 2.4 percent of the tagged Chinook Salmon in the Lower River were classified as having mainstem destinations in the Lower River. Note that the percentages presented here were not weighted according to size-specific tag rates, and are not directly comparable to the weighted percentages. Similarly, from 1976-1984, most Chinook Salmon caught in the Lower River moved into tributaries of the Lower River (summarized in Jennings 1985). In addition, estimated percent distributions of Chinook Salmon were higher, historically, to the Yentna (20.0 percent) and Deshka (35.0 percent) rivers and lower to the Chulitna River (5.0 percent); however, the contribution of Chinook Salmon to the Talkeetna River was similar (Jennings 1985). Chinook Salmon had no Lower River mainstem destinations during historic surveys. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 67 October 2015  Middle River (within and downstream of Devils Canyon) and Tributaries Each year during AEA’s studies, Chinook Salmon tagged in the Lower River moved into Middle River tributaries. Fish used Indian River, and Whiskers, 4th of July and Portage creeks, together accounting for 5.2 percent of the overall destinations of the Lower-River-tagged Chinook Salmon (Figure D-1). In addition, between 0 and 0.5 percent were classified as having mainstem destinations in the Middle River downstream of the canyon. In 2013, two fish (0.3 percent) moved into a tributary destination that was in Devils Canyon (Cheechako Creek). In 2014, one fish went to Cheechako Creek, and one fish had a mainstem destination at the mouth of Cheechako Creek (combined, 0.3 percent of the overall destinations of the 2013 Lower-River-tagged Chinook Salmon, or 0.2 percent as an average of the annual usage over three years; Figure D-1). Similar to 2012-2014, 5.0 percent of the Chinook Salmon caught in the Lower River, from 1976- 1984, had destinations within the Middle River (downstream from Devils Canyon) and tributaries of the Middle River (Jennings 1985).  Middle River (upstream of Devils Canyon), Watana Dam, Upper River and Tributaries. In 2012, two Lower-River-tagged Chinook Salmon passed Devils Canyon and moved into Kosina Creek, an Upper River tributary. These fish represent 0.5 percent of the overall destinations of the 2012 Lower-River-tagged Chinook Salmon, or 0.2 percent as an average of the annual usage over three years (Figure D-1). In 2012-2014, no Lower-River-tagged Chinook Salmon had mainstem destinations upstream of Devils Canyon. In comparison, from 1976-1984, < 0.5 percent of Chinook Salmon had destinations upstream of Devils Canyon (Jennings 1985). 6.1.3.2. Radio-tags applied in Middle River  Lower River and Tributaries Annually, there were Middle-River-tagged Chinook Salmon that moved to tributaries in the Lower River. The main Lower River tributary destinations were the Talkeetna and Chulitna rivers. From 2012-2014, these two tributaries represented an average of 11.3 percent of the overall destinations of the Middle-River-tagged Chinook Salmon (Figure D-2). In 2013, two fish (0.4 percent) were classified to mainstem destinations in the Lower River, but there were none so classified in 2012 or 2014.  Middle River (within and downstream of Devils Canyon) and Tributaries The majority of the Middle-River-tagged Chinook Salmon moved into tributaries in the Middle River downstream of Devils Canyon. Indian River and Portage Creek were the main destinations. From 2012-2014, Indian River and Portage Creek accounted for an average of 26.0 percent and 41.9 percent of the overall destinations of the Middle-River-tagged Chinook Salmon, respectively (Figure D-2). Each year, a few Middle-River-tagged Chinook Salmon travelled to destinations within Devils Canyon, including Cheechako (average 1.0 percent annually) and Chinook (average 0.4 percent annually) creeks. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 68 October 2015 Each year, a portion of the Middle-River-tagged Chinook Salmon were classified as having a mainstem destination, and most of these were located in the Middle River downstream of Devils Canyon. From 2012-2014, Middle-River mainstem destinations ranged from 5.3 to 9.8 percent of the overall destinations of the Middle-River-tagged Chinook Salmon. In 2013, a single fish (0.2 percent) had a mainstem destination within Devils Canyon (in the mainstem proper near the mouth of Chinook Creek), whereas no Middle River-tagged Chinook Salmon had mainstem locations within Devils Canyon in 2012 or 2014.  Middle River (upstream of Devils Canyon), Watana Dam, Upper River and Tributaries. In 2014, two radio-tagged Chinook Salmon passed Devils Canyon, which was down from three in 2013, and 12 in 2012, despite the fact that more large Chinook Salmon were tagged in the Middle River during each successive year (590 in 2014, 536 in 2013, 352 in 2012). From 2012- 2014, tributary use upstream of Devils Canyon averaged 0.2 percent in Devil Creek, 0.1 percent in Tsusena Creek, and 0.5 percent in Kosina Creek (Figure D-2). Wandering behavior was evident in all three years of tagging although the degree that individual fish wandered varied considerably. In 2012-2014, no Middle-River-tagged Chinook Salmon had mainstem destinations upstream of Devils Canyon. 6.1.4. Mainstem Habitat and Tributary Use  Lower River and Tributaries Telemetry results indicated that the majority of Chinook Salmon tagged in the Lower River likely spawned in tributaries. Proportions of tributary spawners were relatively consistent among years for Chinook Salmon (97–99 percent). Chinook Salmon used the Yentna, Deshka, Talkeetna, and Chulitna rivers in all years. From 2012 through 2014, 23 radio-tagged Chinook Salmon were identified with potential spawning sites within mainstem macro habitats of the Lower River (Figure D-11). These radio- tagged fish represented 14 distinct spawning locations (a cluster of points or an isolated point represented one spawning location) of which three were associated with tributary mouths/confluences. None of these potential spawning locations were visually confirmed. Historic surveys only indicated spawning within tributaries and there was no indication of spawning behavior observed at the confluence of tributaries in the Lower River (Barrett et al.1985a,b, Thompson et al.1986).  Middle River (downstream of Devils Canyon) and Tributaries Telemetry results indicated that most of the Chinook Salmon tagged in the Middle River were most likely to have spawned in tributaries. Proportions in 2014 (93 percent) were similar to those estimated in 2012 (90 percent; AEA 2013a) and 2013 (94 percent; AEA 2014c), as was the main suite of Middle River tributary destinations (Indian River, Portage Creek), although relative use of these tributaries varied among years. Analysis of salmon detection histories identified several potential mainstem spawning locations in the Middle River which included the mouths of tributaries, side channels, and main channel habitats. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 69 October 2015 From 2012 to 2014, 100 radio-tagged Chinook Salmon were identified with potential spawning sites within mainstem macrohabitats of the Middle River (Figure D-12; see section 5.4 for a summary of results). Although numerous radio- tagged Chinook Salmon were tracked to mainstem habitats, spawning activity was only confirmed at the confluence of tributary mouths. These results are consistent with studies conducted in the 1980’s where Chinook Salmon were only documented spawning within mainstem habitats at the confluence of five tributary mouths (Chinook, Cheechako, Portage, and 4th of July creeks, and Indian River; Figure D-12; Barrett et al. 1985a,b). Results from three years of tagging, including the number of fish tagged and tracked via fixed stations and mobile surveys, and the number of detections across river segments and habitats, characterize the distribution of salmon throughout the Middle and Lower River. Specifically, the data collected demonstrates that the licensing participant concern (study dispute issue) as to whether radio-telemetry efforts as designed in the Study Plan were sufficient to determine Chinook Salmon spawning sites in the Middle River between PRM102 (confluence of Middle River with Lower River) and PRM124 (Curry) was not realized. During the period 2012 through 2014, 1,681 and 1,478 Chinook Salmon were radio-tagged in the Lower and Middle river, respectively. This resulted in the detection of 88 Lower River origin and 500 Middle River origin Chinook Salmon tags at the Lane Creek telemetry fixed station located at PRM 116.8. Also over these years and over the salmon migration season, a total of 80 complete aerial telemetry surveys were conducted over the river from PRM102 to PRM124. Mobile telemetry surveys resulted in identifying a total of five potential spawning-holding locations for Chinook Salmon in mainstem habitats (tributary confluence), and 10 tags having their destination in tributaries (Lane, Whiskers, and Chase creeks) for that section of river. This compares to a total of 95 potential spawning-holding locations identified for Chinook Salmon in mainstem habitats, and 1,092 in tributaries upstream of PRM124. Radio-tagged Chinook Salmon of Lower River origin had the same opportunity to choose destinations in either section of the river. Further, documented roaming behavior supports that Chinook Salmon of Middle River origin also have substantial opportunity to choose destinations downstream of PRM124. These results indicate that the Middle River upstream of PRM124 includes a substantially higher proportion of spawning (95.0% of mainstem locations, 99.1% of tributaries) than that downstream of PRM124 (5.0% of mainstem locations, 0.9% of tributaries), and is consistent with historical studies (Barrett et al 1985). Therefore, the methodological approach outlined in the Study Plan and respective variances provided sufficient sample size and telemetry detections to evaluate spawning locations for adult Chinook Salmon in the Middle River downstream and upstream of PRM124 and meet the objective of the study.  Middle River (within and upstream of Devils Canyon), Watana Dam, Upper River and Tributaries. No radio-tagged Chinook Salmon were tracked to potential mainstem, slough, or side-channel locations in the Middle River upstream of Devils Canyon. The preponderance of evidence to date suggests that the relatively low numbers (e.g., 50 – 100) of Chinook Salmon in the Upper River are using tributaries for spawning. Tributaries and tributary mouths upstream of Devils STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 70 October 2015 Canyon with documented Chinook Salmon spawning included Cheechako, Fog, Devil, Tsusena, and Kosina Creeks and the Oshetna River. The first evidence of spawning within or upstream of Devils Canyon came in 1982 when ADF&G found adult Chinook Salmon in Cheechako (n = 16) and Chinook (n = 5) creeks, along with redds containing live eggs (ADF&G 1983b). The first scientific observations of Chinook Salmon upstream of Devils Canyon came in 1983, when ADF&G found one adult fish in Devil Creek (ADF&G 1984). In 1984, Chinook Salmon spawning was observed in Chinook (n = 15) and Fog (n = 2) creeks. In 2003, presence of juvenile Chinook Salmon in Fog Creek, Kosina Creek, and the Oshetna River, also implied past spawning activity (Buckwalter 2011). In 2012, 2013 and 2014, radio-tagged Chinook Salmon were detected and appeared to spawn in Kosina, Tsusena or Devil creeks (AEA 2013a, 2014c). In total, AEA tracked 17 Chinook Salmon which migrated above Impediment 3 (Figure F-1 through Figure F-17). 6.1.5. Abundance Estimates  Lower River (RM30) Historically, Chinook Salmon average escapement was 88,000 (range 53,000 to 122,000) in the Lower River at Sunshine Station (i.e., upstream of the Yentna River). This compares closely to an average of 79,000 (approximate range 68,000 to 89,000) for 2013 and 2014 for the Susitna River (upstream of the confluence of the Yentna River, Appendix I).  Middle River (RM120 / Curry) Historically, Chinook Salmon average escapement was 13,000 (range 9,700 to 18,000) for the Middle River at Talkeetna Station. This is 1.8 times higher than an average of 7,100 (range 6,600 to 7,700) for 2013 and 2014 (Appendix I).  Middle River (upstream of Devils Canyon), Watana Dam, and Upper River Recent studies conducted by AEA from 2012 to 2014 provide the only available information on the abundance of Chinook Salmon upstream of Devils Canyon. Radio-telemetry documented Chinook Salmon passage through the canyon and into tributaries, and ARIS sonar made counting of Chinook Salmon passing the Watana Dam site possible. Counts of 12, 3 and 2 radio-tags passed Impediment 3 in 2012, 2013, and 2014 respectively. These counts were used to generate escapement estimates for 2013 and 2014 of 23 and 24 salmon respectively. These three independent metrics indicate that the population of Chinook Salmon upstream of Devils Canyon appears to be on an order of magnitude of <100 adults, with a portion of that upstream of Watana Dam. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 71 October 2015 6.2. Chum 6.2.1. Timing of Migration  Lower River Chum Salmon migrate through the Lower River from early June through at least early September. In 2013 and 2014, Chum Salmon were captured at the Lower River fishwheels (PRM 33-34) from June 11 to August 26, and fish were still being captured on the last day of fishwheel operation in each year so it was likely the run continued beyond these dates. Chum Salmon daily catches peaked on July 20 in 2013 and August 7 in 2014. This migration timing is similar to historic reports with an earlier onset of the run in recent studies. Chum Salmon were captured in the Lower River from June 30 to September 4 during fishwheel operations in 1981 and 1982 (ADF&G 1981, 1983b).  Middle River (downstream of Devils Canyon) Chum Salmon begin their migration through the Middle River about a month later than in the Lower River, early July, and continue through late September. From 2012 to 2014, Chum Salmon were caught at the Middle River as early as July 5 and as late as September 28. This migration timing was similar to the 1980s, when Chum Salmon were caught in fishwheels between as early as July 10 (1983) and as late as September 15 (1981) (Table A-8, Figure A-14). The midpoint of runs as indicated by catch was August 5–12 in 2012 to 2014, and from August 3–17 in the 1980s. 6.2.2. Timing of Spawning  Lower River and Tributaries For Chum Salmon classified to the Yentna, Chulitna or Talkeetna rivers, the 10th and 90th percentile dates when fish were in spawning tributaries spanned from August 7 to September 13. This estimate of spawning timing is shorter by a couple of weeks in comparison with historic data. From 1981 through 1984, the spawning period for Chum Salmon in the Lower River ranged from mid-August through the first week of October, with peak spawning occurring during the last week of August through the first week of September (summarized in Barrett et al. 1985a,b).  Middle River (downstream of Devils Canyon) and Tributaries For spawning Chum Salmon classified to Indian River or Portage Creek, the 10th and 90th percentile dates spanned from August 5 to 30. The 10th and 90th percentile dates for Chum Salmon Middle River mainstem destinations downstream of Devils Canyon ranged from August 8 to September 9 indicating a slightly longer spawning window. However, both of these recent estimates of spawn timing are shorter in comparison with historic estimates. From 1981 through 1984, the spawning period for Chum Salmon in the Middle River ranged from late July through the first week of October, (summarized in Barrett et al. 1985a,b). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 72 October 2015 6.2.3. Distribution to Mainstem and Tributaries 6.2.3.1. Radio-tags applied in Lower River  Lower River and Tributaries In 2012, 400 Chum Salmon tagged in the Lower River moved mostly into Lower River tributaries. The primary Lower River tributary destinations were the Yentna (33.6 percent), Talkeetna (2.1 percent) and Chulitna (9.1 percent) rivers (Figure D-3). In addition, 9.2 percent of the Lower-River-tagged Chum Salmon were classified as having mainstem destinations in the Lower River. No Chum Salmon were tagged in the Lower River in 2013 or 2014. In comparison, historical Chum Salmon tagging results (Merizon et al. 2010) showed that 96 percent had destinations within the Lower River and tributaries of the Lower River. The historical contributions of Chum Salmon to the Yentna (41.0 percent) and Chulitna (4.0 percent) rivers were similar to recent results. Historical Chum Salmon contributions to the Lower River mainstem (26.0 percent) and Talkeetna River (11.0 percent) were higher, as compared to the 2012 tracking results.  Middle River and Tributaries In 2012, a few of the Chum Salmon tagged in the Lower River moved into Middle River tributaries. Fish used Indian River, 4th of July and Portage creeks, together accounting for 4.2 percent of the overall destinations of the Lower-River-tagged Chum Salmon (Figure D-3). In addition, three fish (1.0 percent) were classified as having mainstem destinations in the Middle River. Similarly in 2009, 4.0 percent of the Lower River caught Chum Salmon had a destination in the Middle River and tributaries (downstream of Devils Canyon; Merizon et al. 2010). Tributary use also was similar in a 1984 study, with use of the Indian River, Portage Creek, and 4th of July Creek. However, the proportion of mainstem use was higher historically with more than half of the Chum Salmon having spawning destinations in mainstem habitats of the Middle River (Barrett et al. 1985a,b). No Lower River Chum Salmon had destinations within or upstream of Devils Canyon during recent or historic studies. 6.2.3.2. Radio-tags applied in Middle River  Lower River and Tributaries There were Middle-River-tagged Chum Salmon that moved to tributaries in the Lower River each year from 2012 to 2014. The main Lower River tributary destinations were the Talkeetna and Chulitna rivers. These two tributaries represented an average of 12.7 and 2.6 percent of the overall destinations of the Middle-River-tagged Chum Salmon, respectively (Figure D-4). In three years of tracking, no Middle-River-tagged Chum Salmon were classified to mainstem destinations in the Lower River.  Middle River and Tributaries The majority of the Middle-River-tagged Chum Salmon moved into tributaries in the Middle River downstream of Devils Canyon. Indian River and Portage Creek were the main destinations. From 2012-2014, Indian River and Portage Creek accounted for an average of 27.5 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 73 October 2015 percent and 23.6 percent of the overall destinations of the Middle-River-tagged Chum Salmon, respectively (Figure D-4). Each year, a portion (ranging from 10.4 to 23.9 percent) of the Middle-River-tagged Chum Salmon were classified as having a mainstem destination, all of which were located in the Middle River downstream of Devils Canyon. In three years of tracking, no Middle-River-tagged Chum Salmon had destinations within or upstream of Devils Canyon. 6.2.4. Mainstem Habitat and Tributary Use  Lower River From 2012 to 2014, 31 radio-tagged Chum Salmon were identified with potential spawning sites within mainstem habitat of the Lower River as based on 400 tags applied in the Lower River in 2012, and 4680 tags applied in the Middle River from 2012 – 2014 (Figure D-13). None of the potential spawning locations were visually confirmed. During historic surveys mainstem spawning activity was documented at 33 locations (Barrett et al. 1985a,b). Six of these historic locations were closely associated with a potential spawning location indicated by recent studies (Figure D-13).  Middle River and Tributaries Recent telemetry results indicated that most of the Chum Salmon tagged in the Middle River likely spawned in tributaries. Proportions varied among years: 76 percent in 2012 (AEA 2013a), 90 percent in 2013 (AEA 2014c), and 89 percent in 2014. Although the main suite of Middle- River tributaries was relatively stable (Indian River, Portage Creek), the proportional use of each tributary varied among years. Analysis of salmon detection histories identified several potential mainstem spawning locations in the Middle River. As for the types of mainstem spawning habitats used, Chum Salmon appeared to prefer the mouths of tributaries or side channels. No radio-tagged Chum Salmon were tracked to potential mainstem, slough, or side-channel locations in the Middle River upstream of Devils Canyon or in the Upper River. From 2012 to 2014, 75 radio-tagged Chum Salmon were identified with potential spawning sites in mainstem habitat of the Middle River (Figures D-14 and D-15). Through ground surveys, aerial surveys, and opportunistic visual surveys, spawning was visually confirmed at seven locations indicated by radio telemetry and 16 locations visited opportunistically. Of the visually confirmed spawning locations, 12 were sloughs, six were side channels, and five were tributary mouths/confluences. No Chum Salmon were confirmed spawning in main channel habitats. Chum Salmon were observed present or holding at five potential spawning locations, but spawning behavior was not exhibited. In comparison, historic surveys visually confirmed spawning at 73 locations within mainstem habitats (main channel, side channel, slough, and tributary mouth confluence) of the Middle River. Of all the slough habitats that were visually confirmed as spawning locations during this study, all were also visually confirmed during 1980’s studies (Table 6.2-1). Similarly, all but 13 mainstem spawning locations documented during the 1980’s surveys were closely associated with a potential spawning location indicated by recent radio telemetry analysis (Figures D-14 and D-15). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 74 October 2015 Results from three years of tagging, including the number of fish tagged and tracked via fixed stations and mobile surveys, and the number of detections across river segments and habitats, characterize the distribution of salmon throughout the Middle and Lower River. Specifically, the data collected demonstrates that the license participant concern (study dispute issue) as to whether radio-telemetry efforts as designed in the Study Plan were sufficient to determine Chum Salmon spawning sites in the Middle River between PRM102 (confluence of Middle River with Lower River) and PRM124 (Curry) was not realized. During the period 2012 through 2014, 400 and 680 Chum Salmon were radio-tagged in the Lower and Middle river, respectively. This resulted in the detection of 24 Lower River origin and 215 Middle River origin Chum Salmon tags at the Lane Creek telemetry fixed station located at PRM 116.8. Also, over these years and over the salmon migration season, a total of 80 complete aerial telemetry surveys were conducted over the river from PRM102 to PRM124. Mobile telemetry surveys resulted in identifying a total of three potential spawning-holding locations for Chum Salmon in mainstem habitats (tributary confluence), and 11 tags having their destination in tributaries (Lane Creek, Whiskers Creek) for that section of river. This compares to a total of 72 potential spawning-holding locations identified for Chum Salmon in mainstem habitats, and 378 in tributaries upstream of PRM124. Radio-tagged Chum Salmon of Lower River origin had the same opportunity to choose destinations in either section of the river. Further, documented roaming behavior supports that Chum Salmon of Middle River origin also have substantial opportunity to choose destinations downstream of PRM124. These results indicate that the Middle River upstream of PRM124 includes a substantially higher proportion of spawning (96.0% of mainstem locations, 97.2% of tributaries) than that downstream of PRM124 (4.0% of mainstem locations, 2.8% of tributaries), and is consistent with historical studies (Barrett et al 1985). Therefore, implementation of the Study Plan for radio telemetry including all variances provided sufficient sample size and telemetry detections to evaluate spawning locations for adult Chum Salmon in the Middle River downstream upstream of PRM124 and meet the objective of the study. 6.2.5. Abundance Estimates  Lower River (RM30) Historically, Chum Salmon average escapement was 431,000 (range 263,000 to 765,000) in the Lower River at Sunshine Station (i.e., upstream of the Yentna River). This is lower but modestly similar to an average of 616,000 (range 151,000 to 1,500,000) for 2010 - 2012 for the Susitna River (upstream of the confluence of the Yentna River; Cleary et al. 2013, Cleary et al. in prep A, Cleary et al. in prep B).  Middle River (RM120 / Curry) and Tributaries Historically, Chum Salmon average escapement was 28,000 (range 13,000 to 49,000) for the Middle River at Talkeetna Station. This is very similar to an average of 26,000 for 2012 - 2014 using the proportion of radio-tags with a destination in the Middle River (4.2 percent of the Lower River escapement). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 75 October 2015 License participants raised the concern as to whether use of existing and recent escapement information (2010 - 2012) in combination with new distribution data in the Middle River (2012 – 2014) as designed in the Study Plan were sufficient to evaluate Chum Salmon distribution and abundance. AEA completed three years of radio-tagging on Chum Salmon in the Middle River, and one year in the Lower River. In combination, these data produce the recent average abundance estimate presented above and meet the objective of the study. 6.3. Coho 6.3.1. Timing of Migration  Lower River Based on fishwheel catches, the migration timing of Coho Salmon through the Lower River ranged from the end of June to early September. In 2013 and 2014 Coho Salmon were captured from June 28 to August 31. In comparison, Coho Salmon collections were later in 1981 and 1982, ranging July 4 to September 4 (ADF&G 1981, 1983b).  Middle River (downstream of Devils Canyon) The timing of migration for Coho Salmon through the Middle River extends from late July to at least the end of September. In 2014, the first Coho Salmon was captured at the Middle River fishwheels on July 22, which was similar to 2013 (July 23) but 6 days earlier than in 2012 (July 28; Table A-8, Figure A-15). In the 1980s, the beginning of Coho Salmon collections was between July 18 (1984) and August 4 (1981). The midpoint of catches in 2014 (August 20) occurred 5 days later than in 2012 and 2013 (August 15). In the 1980s, the midpoint of catches ranged from August 12–23. The last Coho Salmon was captured on September 30 in 2014. Also, sonar data collected at Curry indicated that salmon-sized fish (presumably Chum and Coho Salmon) continued to migrate past Curry through the end of September in 2014 (Figures A-19 and A-20). Prior to 2014, the latest that a Coho Salmon had been captured at the Middle River fishwheels was September 21 (2013), although the fishwheels were shut down for the season between September 12 and September 21 in the 1980s. 6.3.2. Timing of Spawning  Lower River and Tributaries For Coho Salmon classified to the Yentna, Deshka, Chulitna or Talkeetna rivers, the 10th and 90th percentile dates when fish were in assigned spawning tributaries spanned from August 7 to September 23. Spawn timing in the mainstem appeared similar with the 10th and 90th percentile dates for Coho Salmon Lower River mainstem destinations ranging from August 9 to September 30. This spawning timing appears to be protracted and shifted earlier in comparison with historic data. From 1981-1984, the spawning period for Coho Salmon in the Lower River ranged from late September through mid-October, with peak spawning occurring during the last week of September (summarized in Barrett et al. 1985a,b). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 76 October 2015  Middle River (downstream of Devils Canyon) and Tributaries For Coho Salmon classified to Indian River or Portage Creek, the 10th and 90th percentile dates when fish were in assigned spawning tributaries spanned from August 20 to October 9. The 10th and 90th percentile dates for Coho Salmon Middle River mainstem destinations downstream of Devils Canyon ranged from August 20 to September 26. This spawn timing appears to be shifted earlier than historically. During the 1980s, the spawning period for Coho Salmon in the Susitna River ranged from late September through mid-October, with peak spawning occurring during the last week of September (summarized in Barrett et al. 1985a,b). 6.3.3. Distribution to Mainstem and Tributaries 6.3.3.1. Radio-tags applied in Lower River  Lower River and Tributaries During 2012-2014, Coho Salmon tagged in the Lower River moved mostly into Lower River tributaries. The primary Lower River tributary destinations were the Yentna (22.8 percent), Deshka (12.1 percent), Talkeetna (11.6 percent) and Chulitna (24.8 percent) rivers (Figure D-5). In addition, 2.8 to 6 percent of the Lower-River-tagged Coho Salmon were classified as having mainstem destinations in the Lower River. Similarly, Merizon et al. (2010) estimated that most (98 percent) Coho Salmon caught in the Lower River had destination in the Lower River and tributaries. Using data collected in the 1980’s and 2009, the distribution of Coho Salmon to the Yentna River ranged from 23.0 percent (1980’s) to 47.0 percent (2009). The Talkeetna and Chulitna rivers had a combined contribution of 29.0 (1980’s) to 34.0 (2009) percent. The remaining Lower River, including the Deshka River had contributions of 27.0 (2009) to 46.0 percent (1980’s; Barrett et al. 1985a, b; Merizon et al. 2010).  Middle River and Tributaries Each year during recent studies, Coho Salmon tagged in the Lower River moved into Middle River tributaries. Fish used Indian River, and Whiskers, Chase, Lane and Portage creeks, together accounting for 2.6 percent of the overall destinations of the Lower-River-tagged Coho Salmon (Figure D-5). In addition, between 0 and 0.6 percent were classified as having mainstem destinations in the Middle River. In comparison, of the estimated Coho Salmon that entered the Middle River from 1981-1984, most had destinations in Gash, Whiskers, and Chase creeks and Indian River (Barrett et al. 1985a, b). No Coho Salmon from recent or historic studies had destination upstream of Devils Canyon. 6.3.3.2. Radio-tags applied in Middle River  Lower River and Tributaries There were Middle-River-tagged Coho Salmon that moved to tributaries in the Lower River during each year of recent telemetry studies. The main Lower River tributary destinations were the Talkeetna and Chulitna rivers. From 2012-2014, these two tributaries represented an average of 5.6 and 13.4 percent of the overall destinations of the Middle-River-tagged Coho Salmon, respectively (Figure D-6). In three years of tracking, there were four Middle-River-tagged Coho STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 77 October 2015 Salmon (annual percentages ranged from 0 to 1.7 percent) that were classified to mainstem destinations in the Lower River.  Middle River and Tributaries The majority of the Middle-River-tagged Coho Salmon moved into tributaries in the Middle River downstream of Devils Canyon. Indian River and Portage Creek were the main destinations. From 2012-2014, Indian River and Portage Creek accounted for an average of 37.6 percent and 9.0 percent of the overall destinations of the Middle-River-tagged Coho Salmon, respectively (Figure D-6). Each year, a portion (ranging from 5.4 to 15.9 percent) of the Middle- River-tagged Coho Salmon were classified as having a mainstem destination in the Middle River downstream of Devils Canyon. In three years of tracking, no Middle-River-tagged Coho Salmon had destinations within or upstream of Devils Canyon. 6.3.4. Mainstem Habitat and Tributary Use  Lower River and Tributaries Telemetry results indicated that the majority of Coho Salmon tagged in the Lower River likely spawned in tributaries. Proportions of tributary spawners were relatively consistent among years for Coho Salmon (93–96 percent). Coho Salmon used the Yentna, Deshka, Talkeetna, and Chulitna rivers in all years. From 2012 to 2014, 40 radio-tagged Coho Salmon were identified with potential spawning sites in mainstem habitats of the Lower River (Figure D-16). Seven suspected spawning locations were at tributary mouths/confluences and the remaining were either main channel, side channel, or slough locations. None of these potential spawning locations were visually confirmed for spawning. During surveys conducted in the 1980’s, six mainstem spawning locations were visually confirmed for Coho Salmon. Five of these historic spawning locations were closely associated with locations indicated from 2012- 2014 analyses.  Middle River and Tributaries Telemetry results indicated that most of the Coho Salmon tagged in the Middle River likely spawned in tributaries. Proportions in 2014 (94 percent) were similar to those estimated in 2012 (84 percent; AEA 2013a) and 2013 (89 percent; AEA 2014c). The specific tributary destinations were also similar, although relative use of these tributaries varied among years. Analysis of salmon detection histories identified several potential mainstem spawning locations in the Middle River. As for the types of mainstem spawning habitats used, Coho Salmon appeared to prefer the mouths of tributaries or side channels. No radio-tagged Coho Salmon were tracked to potential mainstem, slough, or side-channel locations in the Middle River upstream from Devils Canyon or in the Upper River. From 2012 to 2014, 35 radio-tagged Coho Salmon were identified with potential spawning sites in mainstem habitat of the Middle River (Figure D-17). Through ground surveys, aerial surveys, and opportunistic visual surveys, spawning was confirmed at three locations visited opportunistically. Of the visually-confirmed spawning locations one was a slough, and two were at tributary mouths/confluences (Skull and Whiskers creeks). Coho Salmon were present or STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 78 October 2015 holding at two locations (Indian River and Portage Creek) indicated by radio telemetry analysis. During spawning surveys conducted in the 1980’s, 12 mainstem spawning locations were visually confirmed for Coho Salmon and seven were closely associated with a location indicated by radio telemetry analyses. Results from three years of tagging, including the number of fish tagged and tracked via fixed stations and mobile surveys, and the number of detections across river segments and habitats, characterize the distribution of salmon throughout the Middle and Lower River. Specifically, the data collected demonstrates that the licensing participant concern (study dispute issue) as to whether radio-telemetry efforts as designed in the Study Plan were sufficient to determine Coho Salmon spawning sites in the Middle River between PRM102 (confluence of Middle River with Lower River) and PRM124 (Curry) was not realized. During the period 2012 through 2014, 1,653 and 638 Coho Salmon were radio-tagged in the Lower and Middle river, respectively. This resulted in the detection of 58 Lower River origin and 315 Middle River origin Coho Salmon tags at the Lane Creek telemetry fixed station located at PRM 116.8. Also over these years and over the salmon migration season, a total of 80 complete aerial telemetry surveys were conducted over the river from PRM102 to PRM124. Mobile telemetry surveys resulted in identifying a total of four potential spawning-holding locations for Coho Salmon in mainstem habitats (tributary confluence), and 61 tags having their destination in tributaries (Lane, Whiskers, Chase, Stash, and Gash creeks) for that section of river. This compares to a total of 31 potential spawning-holding locations identified for Coho Salmon in mainstem habitats, and 320 in tributaries upstream of PRM124. Radio-tagged Coho Salmon of Lower River origin had the same opportunity to choose destinations in either section of the river. Further, documented roaming behavior supports that Coho Salmon of Middle River origin also have substantial opportunity to choose destinations downstream of PRM124. These results indicate that the Middle River upstream of PRM124 includes a substantially higher proportion of spawning (88.6% of mainstem locations, 84.0 % of tributaries) than that downstream of PRM124 (11.4% of mainstem locations, 16.0% of tributaries), and is consistent with historical studies (Barrett et al 1985). Therefore, implementation of the Study Plan for radio telemetry including all variances provided sufficient sample size and telemetry detections to evaluate spawning locations for adult Coho Salmon in the Middle River downstream and upstream of PRM124 and meet the objective of the study. 6.3.5. Abundance Estimates  Lower River (RM30) Historically, Coho Salmon average escapement was 44,000 (range 15,000 to 95,000) in the Lower River at Sunshine Station (i.e., upstream of the Yentna River). This is almost half of an average of 102,000 (range 73,000 to 132,000) for the period 2010 - 2014 for the Susitna River (upstream of the confluence of the Yentna River; Cleary et al. 2013, Cleary et al. in prep A, Cleary et al. in prep B, Appendix I). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 79 October 2015  Middle River (RM120 / Curry) and Tributaries Historically, Coho Salmon average escapement was 1,600 (range 800 to 2,400) for the Middle River at Talkeetna Station. This is approximately on quarter of recent estimate of 7,200 (range 6,200 to 8,300) based on 2013 and 2014 data (Cleary et al. 2013, Cleary et al. in prep A, Cleary et al. in prep B, Appendix I). 6.4. Pink 6.4.1. Timing of Migration  Lower River Pink Salmon migrate through the Lower River from late June to the end of August. In 2013 and 2014, Pink Salmon were captured at the Lower River fishwheels from June 23 to August 25. This was very similar to historic timing from the early 1980s. In 1981 and 1982, Pink Salmon catches occurred from June 28 to August 30 (ADF&G 1981, 1983b) and in 1985Pink Salmon were caught at the Flathorn fishwheels from June 22 to September 1 (Thompson et al. 1986).  Middle River (downstream of Devils Canyon) From 2012 to 2014, Pink Salmon were captured at the Middle River fishwheels as early as July 8 and as late as September 4 (Table A-8, Figure A-16). In the 1980s, Pink Salmon were caught with a similar migration timing, as early as July 7 in 1984 to as late as August 29 in 1981 and 1984. The midpoint of catches from 2012 to 2014 ranged from July 31 to August 8, which was similar to those observed in the 1980s (August 1–8). 6.4.2. Timing of Spawning  Lower River and Tributaries For Pink Salmon classified to the Yentna, Deshka, Chulitna or Talkeetna rivers, the 10th and 90th percentile dates when fish were in spawning tributaries spanned from August 6 to 29. The 10th and 90th percentile dates for Pink Salmon Lower River mainstem destinations ranged from July 31 to August 30. In comparison, from 1981 through1984, the spawning period for Pink Salmon in the Lower River was similar. Historic Pink Salmon spawning ranged from late July through early September, with peak spawning occurring during the first two weeks of August (summarized in Barrett et al. 1985a, b).  Middle River (downstream of Devils Canyon) and Tributaries For Pink Salmon classified to Indian River or to 4th of July or Portage creeks for spawning, the 10th and 90th percentile dates when fish were in the spawning tributaries spanned from July 31 to August 20. The 10th and 90th percentile dates for Pink Salmon Middle River mainstem destinations downstream of Devils Canyon ranged from August 5 to 27. In comparison the historic spawn timing was similar from 1981 through 1984, the spawning period for Pink Salmon in the Middle River ranged from late July through early September, with peak spawning occurring during the second and third week of August (summarized in Barrett et al. 1985a,b). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 80 October 2015 6.4.3. Distribution to Mainstem and Tributaries 6.4.3.1. Radio-tags applied in Lower River  Lower River and Tributaries During 2012-2014, Pink Salmon tagged in the Lower River moved mostly into Lower River tributaries. The main Lower River tributary destinations were the Yentna (21.6 percent), Deshka (12 percent), Talkeetna (5 percent) and Chulitna (21.3 percent) rivers, and Willow (7 percent) and Montana (5.5 percent) creeks (Figure D-7). In addition, 0.6 to 14.7 percent of the Lower- River-tagged Pink Salmon were classified as having a mainstem destination in the Lower River. Similarly, Barrett et al. (1985a, b) indicated that, historically, most of the Pink Salmon caught in the Lower River also had destinations in the Lower River and Tributaries. In 1984, this included about 10.0 percent to the Yentna, 60.0 percent to tributaries between the Yentna and Sunshine Station, and the remaining 30.0 percent had destination upstream of Sunshine Station.  Middle River and Tributaries Each year, Pink Salmon tagged in the Lower River moved into Middle River tributaries. Fish used Indian River, and Lane, 4th of July, Gold and Portage creeks, together accounting for 3 percent of the overall destinations of the Lower-River-tagged Pink Salmon (Figure D-7). In 2013, one fish (0.9 percent) was classified as having mainstem destinations in the Middle River (at the mouth of 4th of July Creek). Similarly, from 1981-1984, most Pink Salmon with destinations in the Middle River ended up in tributaries. The three most important tributary destinations were tributaries used in recent years, Indian River, 4th of July Creek and Lane Creek (Barrett et al. 1985a,b). No Lower-River-tagged Pink Salmon had destinations within or upstream of Devils Canyon either historically or in recent studies. 6.4.3.2. Radio-tags applied in Middle River  Lower River and Tributaries Annually, there were Middle-River-tagged Pink Salmon that moved to tributaries in the Lower River during each year of recent studies. The main Lower River tributary destinations were the Talkeetna and Chulitna rivers. From 2012-2014, these two tributaries represented an average of 11.7 and 2.2 percent of the overall destinations of the Middle-River-tagged Pink Salmon, respectively (Figure D-8). In three years of tracking, no Middle-River-tagged Pink Salmon were classified to mainstem destinations in the Lower River.  Middle River and Tributaries The majority of the Middle-River-tagged Pink Salmon moved into tributaries in the Middle River downstream of Devils Canyon. Indian River and 4th of July and Portage creeks were the main destinations, accounted for an average of 34.7, 9.6 and 10.7 percent of the overall destinations of the Middle-River-tagged Pink Salmon, respectively (Figure D-8). Each year, a portion (ranging from 5.5 to 9.0 percent) of the Middle-River-tagged Pink Salmon were classified as having a mainstem destination, all of which were located in the Middle River STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 81 October 2015 downstream of Devils Canyon. In three years of tracking, no Middle-River-tagged Pink Salmon had destinations within or upstream of Devils Canyon. 6.4.4. Mainstem Habitat and Tributary Use  Lower River and Tributaries Telemetry results indicated that the majority of Pink Salmon tagged in the Lower River likely spawned in tributaries. Proportions of tributary spawners were more variable for Pink Salmon (84–97 percent) compared to Chinook or Coho salmon. Pink Salmon used the Yentna and Deshka rivers in all three years, and used Willow and Montana creeks and Talkeetna and Chulitna rivers to extents that varied among years (AEA 2013a, 2014c). In contrast to 2013, when 16 percent of the Lower River Pink Salmon were classified to mainstem destinations (AEA 2014c), 1–3 percent were so classified in 2012 and 2014 (AEA 2013a). The difference can most likely be explained by lower tracking effort in tributaries in 2013, thus biasing Pink Salmon classifications away from tributary designations, and creating the appearance of a higher proportion of mainstem spawning. With higher effort of tributary tracking in 2012 and 2014, the mainstem spawning results were likely more realistic than in 2013. From 2012 through 2014, 12 radio-tagged Pink Salmon were identified with potential spawning sites within mainstem habitats of the Lower River (Figure D-18). Of these, eight were associated with tributary mouths/confluences. Spawning was not visually confirmed for any Lower River potential spawning locations for Pink Salmon. Similarly, during spawning surveys conducted in the 1980’s, Pink Salmon spawning was not observed within mainstem habitats of the Lower River.  Middle River and Tributaries Telemetry results indicated that most of the Pink Salmon tagged in the Middle River likely spawned in tributaries. Proportions in 2014 (93 percent) were similar to those estimated in 2012 (94 percent; AEA 2013a) and 2013 (91 percent; AEA 2014c), as were the primary Middle River tributary destinations (4th of July Creek, Indian River, Portage Creek), although the relative use of these tributaries varied among years. Analysis of salmon detection histories identified several potential mainstem spawning locations in the Middle River. As for the types of mainstem spawning habitats used, Pink Salmon appeared to prefer the mouths of tributaries or side channels. No radio-tagged Pink Salmon were tracked to potential mainstem, slough, or side- channel locations in the Middle River upstream of Devils Canyon nor in the Upper River. From 2012 through 2014, 21 radio-tagged Pink Salmon were identified with potential spawning sites within mainstem habitats of the Middle River (Figure D-19). Spawning was visually confirmed at three locations (Indian River Mouth, 4th of July Creek Mouth, and Lane Creek Mouth) as indicated by radio telemetry and at three additional locations (Portage, Gold, and 5th of July creek mouths) visually confirmed during opportunistic surveys. Spawning surveys conducted in the 1980’s visually confirmed spawning by Pink Salmon at twenty locations; four were tributary mouth confluences (Fourth of July, Skull, and Portage creeks and Indian River) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 82 October 2015 and 16 were slough habitats (Table 6.2-1). Nine of the historic spawning locations were closely associated with potential spawning locations indicated by recent studies. Results from three years of tagging, including the number of fish tagged and tracked via fixed stations and mobile surveys, and the number of detections across river segments and habitats, characterize the distribution of salmon throughout the Middle and Lower River. Specifically, the data collected demonstrates that the licensing participant concern (study dispute issue) as t o whether radio-telemetry efforts as designed in the Study Plan were sufficient to determine Pink Salmon spawning sites in the Middle River between PRM102 (confluence of Middle River with Lower River) and PRM124 (Curry) was not realized. During the period 2012 through 2014, 796 and 631 Pink Salmon were radio-tagged in the Lower and Middle river, respectively. This resulted in the detection of 28 Lower River origin and 328 Middle River origin Pink Salmon tags at the Lane Creek telemetry fixed station located at PRM 116.8. Also over these years and over the salmon migration season, a total of 80 complete aerial telemetry surveys were conducted over the river from PRM102 to PRM124. Mobile telemetry surveys resulted in identifying a total of three potential spawning-holding locations for Pink Salmon in mainstem habitats (tributary confluence), and 39 tags having their destination in tributaries (Lane and Whiskers creeks) for that section of river. This compares to a total of 18 potential spawning-holding locations identified for Pink Salmon in mainstem habitats, and 373 in tributaries upstream of PRM124. Radio-tagged Pink Salmon of Lower River origin had the same opportunity to choose destinations in either section of the river. Further, documented roaming behavior supports that Pink Salmon of Middle River origin also have substantial opportunity to choose destinations downstream of PRM124. These results indicate that the Middle River upstream of PRM124 includes a substantially higher proportion of spawning (85.7% of mainstem locations, 90.5% of tributaries) than that downstream of PRM124 (14.3% of mainstem locations, 9.5% of tributaries), and is consistent with historical studies (Barrett et al 1985). Therefore, implementation of the Study Plan for radio telemetry including all variances provided sufficient sample size and telemetry detections to evaluate spawning locations for adult Pink Salmon in the Middle River downstream and upstream of PRM124 and meet the objective of the study. 6.4.5. Abundance Estimates  Lower River (RM30) and Middle River (RM120 / Curry) Historically, Pink Salmon average escapement was 388,000 (range 40,000 to 1,017,000) in the Lower River at Sunshine Station (i.e., upstream of the Yentna River), and average escapement was 46,000 (range 1,000 to 117,000) for the Middle River at Talkeetna Station. No escapement estimates were made for recent studies. Licensing participants posited that an escapement estimate was needed for Pink Salmon (FERC 2013b). In the 1980’s Pink Salmon abundance estimates varied 120- to 50-fold from year to year and seven-fold between even year returns (ADF&G 1983b, 1985a,b). Recent data from ADF&G’s Deshka River weir showed that even year returns varied 40-fold from 2004-2012 (AEA 2013b). Due to this wide inter-annual variation documented for Pink Salmon abundance, STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 83 October 2015 two years of abundance estimates would provide little, or no, additional value for determining impacts of project developments. 6.5. Sockeye 6.5.1. Timing of Migration  Lower River Sockeye Salmon migrate through the Lower River from approximately late May to the start of September. In recent years (2013 and 2014), Sockeye Salmon were captured at the Lower River fishwheels from June 4 to August 26. This migration timing is similar to historic records. Based on Lower River fishwheel catches from 1982 to 1984, the migration timing for the first run of Sockeye Salmon ranged from May 26 - June 28 with peak migration occurring during mid-June (Barrett et al. 1985a,b; Thompson et al. 1986). Similarly, fishwheel catches from 1981-1984 describe the migration timing for the second run of Sockeye Salmon as occurring from June 27 (1982) to September 12 (1982) with peak migrations occurring from mid-to late July (Barrett et al. 1985,a,b; Thompson et al. 1986)  Middle River (downstream of Devils Canyon) Migration timing for Sockeye Salmon in the Middle River has been relatively consistent across the years. From 2012 to 2014, Sockeye Salmon were captured between June 15 and September 10 (Table A-8, Figure A-17). This run timing and duration was similar to the 1980s, when the earliest that a Sockeye Salmon was captured was June 26 (1984) and the latest was September 18 (1982). Consistent with 2012 and 2013, catches of Sockeye Salmon in 2014 were relatively low and sporadic through July and August. The midpoint of catch in 2014 (August 1) was consistent with previous records, although earlier than 1981 to 1983, 1985, and 2013 (range: August 5–7), and similar to 1984 and 2012 (range: July 30 to August 1). 6.5.2. Timing of Spawning  Lower River and Tributaries For Sockeye Salmon classified as spawning in the Yentna, Chulitna or Talkeetna rivers, the 10th and 90th percentile dates when fish were spawning spanned from August 7 to September 13. From 2012-2014, three Sockeye Salmon were classified as having mainstem destinations in the Lower River. The spawn timing for the three fish was earlier than that estimated for tributary spawning (July 17 and 25) but was similar to historic timing. In 1981 through 1984, the spawning period for Sockeye Salmon in the Lower River ranged from mid-to late July for the first run and from late August through early October for the second run. Peak spawning for the second run occurred during the last week of August through mid-September (summarized in Barrett et al. 1985a, b).  Middle River (downstream of Devils Canyon) and Tributaries For Sockeye Salmon classified as spawning in Indian River or Portage Creek, the 10th and 90th percentile dates when fish were in the spanned from July 26 to September 7. The 10th and 90th STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 84 October 2015 percentile dates for Sockeye Salmon Middle River mainstem destinations downstream of Devils Canyon were later than in tributaries and occurred over a shorter duration. Mainstem dates ranged from August 16 to September 20 during recent studies. Historic data indicates that Middle River Sockeye Salmon spawning was more similar to timing of recent mainstem spawners and was protracted as spawning continued into October. From 1981 through 1984, the spawning period for Sockeye Salmon in the Susitna River ranged from late August through early October, with the peak spawning occurring during the last week of August through mid- September (summarized in Barrett et al. 1985a, b). 6.5.3. Distribution to Mainstem and Tributaries 6.5.3.1. Radio-tags applied in Lower River  Lower River and Tributaries. In 2012, 100 Sockeye Salmon tagged in the Lower River moved mostly into Lower River tributaries. The overwhelming majority moved into the Yenta River (96 percent), however fish also used Deshka (1 percent) and Chulitna (2 percent) rivers (Figure D-9). None of the Lower- River-tagged Sockeye Salmon were classified as having mainstem destinations in the Lower River. Similarly, most Sockeye Salmon destinations from historic surveys in the Lower River were within tributaries (Fair 2009; Yanusz et al. 2011a, b). The Yentna River received up to 80.0 percent of the Sockeye Salmon distribution while the Talkeetna and Chulitna river basins followed in importance with 14.5 and 5.0 percent distribution, respectively. In the 1980s, however, the Yentna River was estimated as having 48.0 percent of the Sockeye Salmon distribution while the Talkeetna and Chulitna Rivers made up the 46.0 percent (ADFG 1981, 1982; Barrett et al. 1985a, b; Thompson et al. 1986).  Middle River and Tributaries Sockeye Salmon tagged in the Lower River in 2012 did not use any Middle River tributaries. However, a single fish (1 percent) was classified as having a mainstem destination in the Middle River. Historically, about 1.0 (1980’s) to 2.0 (2007-2008) percent of Sockeye Salmon had destinations within the Middle River and tributaries (ADFG 1981, 1982; Barrett et al. 1985a,b; Thompson et al. 1986; Fair 2009; Yanusz et al. 2011a,b). Of the Sockeye Salmon that had destinations in the Middle River, 98.0 percent had mainstem destinations (sloughs) while < 1.0 percent had a destination in Indian River. No Sockeye Salmon, from either the recent or historic data set, had destinations within or upstream of Devils Canyon. 6.5.3.2. Radio-tags applied in Middle River  Lower River and Tributaries There were Middle-River-tagged Sockeye Salmon that moved to tributaries in the Lower River during each year of recent studies. The main Lower River tributary destinations were the Talkeetna and Chulitna rivers. From 2012-2014, these two tributaries represented an average of 10.2 and 9.2 percent of the overall destinations of the Middle-River-tagged Sockeye Salmon, respectively (Figure D-10). In 2013 three fish (3.3 percent) were classified to mainstem destinations in the Lower River, but there were none so classified in 2012 or 2014. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 85 October 2015  Middle River and Tributaries Middle-River-tagged Sockeye Salmon were the most likely group to be classified with a mainstem destination in the Middle River, with annual proportions ranging from 46.5 to 78.7 percent in 2012-2014. Many of the remaining fish moved into tributaries in the Middle River downstream from Devils Canyon. Indian River, and 4th of July and Portage creeks were the main destinations, accounting for an average of 3.9, 1.3 and 4.9 percent of the overall destinations of the Middle-River-tagged Sockeye Salmon, respectively (Figure D-10). In three years of tracking, no Middle-River-tagged Sockeye Salmon had destinations within or upstream of Devils Canyon. 6.5.4. Mainstem Habitat and Tributary Use  Lower River and Tributaries From 2012 through 2014, no radio-tagged Sockeye Salmon were identified with potential spawning sites in mainstem habitats of the Lower River (based on 100 tags applied in the Lower River in 2012, and 409 tags applied in the Middle River from 2012 – 2014). Similarly, spawning surveys conducted during the 1980’s found no evidence of Sockeye Salmon spawning in Lower River mainstem habitats.  Middle River and Tributaries For Sockeye Salmon, the percent tracked into tributaries varied among years (21–54 percent), but never approached the levels seen for other salmon species in the Middle River, or for Sockeye Salmon in the Lower River. Primary Middle River tributaries used included Indian River and Portage Creek. Analysis of salmon detection histories identified several potential mainstem spawning locations in the Middle River. As for the types of mainstem spawning habitats used, Sockeye Salmon showed a stronger preference for sloughs. Of particular interest to Sockeye Salmon in all three years were Slough 8A, Slough 9, and Slough 11 (e.g., in 2014, 46 spawning Sockeye Salmon were classified to these three sloughs, i.e., 32 percent of the number classified to a destination, and 70 percent of the total number that were classified to a mainstem destination). No radio-tagged Sockeye Salmon were tracked to potential mainstem, slough, or side-channel locations in the Middle River upstream of Devils Canyon or in the Upper River. From 2012 through 2014, 104 radio-tagged Sockeye Salmon were identified with potential spawning sites within mainstem habitats of the Middle River. Ten locations indicated by radio telemetry were visually confirmed for spawning by Sockeye Salmon (Figure D-20). This included nine sloughs (Table 6.2-1) and one side channel (Side Channel 21). Sockeye Salmon were present or holding at two locations (Portage Creek Mouth and 4th of July Side Channel), but spawning behavior was not observed. Additionally, opportunistic surveys visually confirmed spawning at two locations. Spawning surveys conducted in the 1980’s visually confirmed 27 mainstem spawning locations for Sockeye Salmon (Figure D-20; Table 6.2-1). Of these, 23 were within slough habitats, three were within main channel or side channel habitats, and one was at the confluence of a tributary mouth (Portage Creek). Thirteen of the 27 historic spawning locations for Sockeye Salmon were closely associated with a potential spawning location indicated by radio telemetry. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 86 October 2015 Results from three years of tagging, including the number of fish tagged and tracked via fixed stations and mobile surveys, and the number of detections across river segments and habitats, characterize the distribution of salmon throughout the Middle and Lower River. Specifically, the data collected demonstrates that the licensing participant concern (study dispute issue) as to whether radio-telemetry efforts as designed in the Study Plan were sufficient to determine Sockeye Salmon spawning sites in the Middle River between PRM102 (confluence of Middle River with Lower River) and PRM124 (Curry) was not realized. During the period 2012 through 2014, 100 and 409 Sockeye Salmon were radio-tagged in the Lower and Middle river, respectively. This resulted in the detection of three Lower River origin and 237 Middle River origin Sockeye Salmon tags at the Lane Creek telemetry fixed station located at PRM 116.8. Also over these years and over the salmon migration season, a total of 80 complete aerial telemetry surveys were conducted over the river from PRM102 to PRM124. Mobile telemetry surveys resulted in identifying one potential spawning/holding location for Sockeye Salmon and zero tags having their destination in tributaries for that section of river. This compares to a total of 104 potential spawning-holding locations identified for Sockeye Salmon in mainstem habitats, and 41 in tributaries upstream of PRM124. Radio-tagged Sockeye Salmon of Lower River origin had the same opportunity to choose destinations in either section of the river. Further, documented roaming behavior supports that Sockeye Salmon of Middle River origin also have substantial opportunity to choose destinations downstream of PRM124. These results indicate that the Middle River upstream of PRM124 includes a substantially higher proportion of spawning (100% of mainstem locations, 97.6% of tributaries) than that downstream of PRM124 (0% of mainstem locations, 2.4% of tributaries), and is consistent with historical studies (Barrett et al 1985). Earlier Sockeye Salmon studies, applying ~550 radio-tags to Sockeye Salmon in the Lower River (Yanusz et al. 2011a,b), did not track any of those fish to destinations in the Middle River downstream of PRM124. Therefore, implementation of the Study Plan for radio telemetry including all variances and other recent studies provided sufficient sample size and telemetry detections to evaluate spawning locations for adult Sockeye Salmon in the Middle River downstream and upstream of PRM124 and meet the objective of the study. 6.5.5. Abundance Estimates  Lower River (RM30) Historically, Sockeye Salmon average escapement was 122,000 (range 71,000 to 152,000) in the Lower River at Sunshine Station (i.e., upstream of the Yentna River). This is higher than but modestly comparable to an average of 88,000 (range 70,000 to 107,000) for 2006 - 2008 for the Susitna River (upstream of the confluence of the Yentna River; Yanusz et al. 2007, 2011a, b).  Middle River (RM120 / Curry) and Tributaries Historically, Sockeye Salmon average escapement was 2,400 (range 1,300 to 3,600) for the Middle River at Talkeetna Station. This is very similar to an average of 2,000 for 2007 - 2008 using the proportion of radio-tags with a destination in the Middle River (2.4 percent of the Lower River escapement). None of the radio-tags applied in the Lower River in 2012 had a destination in the Middle River. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 87 October 2015 License participants raised the concern as to whether using existing and recent escapement information (2006 - 2008) in combination with new distribution data in the Middle River (2012 – 2014) as designed in the Study Plan were sufficient to evaluate Sockeye Salmon distribution and abundance. AEA completed three years of radio-tagging on Sockeye Salmon in the Middle River, and one year in the Lower River. In combination, these data produced confirming information of the distribution of sockeye salmon and the recent average abundance estimate presented above and met the objective of the study. License participants raised the concern as to whether a contemporary escapement estimate was not needed for Sockeye Salmon (FERC 2013b). ADFG released a total of 1,524 radio tags in Sockeye Salmon from 2006-2008 which provided Susitna River basin wide distribution and abundances (327,732 – 418,197) for each year (Yanusz et al. 2007, 2011a,b). Furthermore, recent research were consistent with historical data from the 1980’s, as well as AEA studies which demonstrated the low proportional contribution of Sockeye Salmon to the Middle River relative to the Susitna River basin. 7. CONCLUSIONS From 2012 to 2014, the study team completed three consecutive years of adult salmon escapement studies on the Susitna River. The 2012 Adult Salmon Distribution and Habitat Utilization Study (AEA 2012) was an AEA-sponsored initiative that successfully met all seven study objectives and helped to refine the scope and methods of the 2013–2014 studies. The 2013 Salmon Escapement Study (Study 9.7; AEA 2014c) met seven of the eight study objectives outlined in RSP Section 9.7.1.2 and adopted both of the modifications outlined in FERC’s February 1 SPD as part of the approved Study Plan. The 2014 Salmon Escapement Study (Study 9.7), as reported herein and earlier by AEA (2014a), successfully met all eight study objectives as outlined in the Study Plan. The study team has completed all field work, data analysis, and reporting related to the Salmon Escapement Study (Study 9.7). 8. LITERATURE CITED AEA. 2012. Revised Study Plan: Susitna-Watana Hydroelectric Project FERC Project No. 14241. December 2012. Prepared for the Federal Energy Regulatory Commission by the Alaska Energy Authority, Anchorage, Alaska. http://www.susitna-watanahydro.org /study-plan. AEA. 2013a. Adult Salmon Distribution and Habitat Utilization Study. Susitna-Watana Hydroelectric Project, FERC Project No. 14241. February 2013. Anchorage, Alaska. http://www.susitna-watanahydro.org/wp-content/uploads/2013/03/Attachment-A-AS.pdf AEA. 2013b. Synthesis of Existing Fish Population Data. Susitna-Watana Hydroelectric Project, FERC Project No. 14241. February 2013. Prepared by R2 Research Associates for Alaska Energy Authority, February 2013. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 88 October 2015 AEA. 2014a. 2014 Implementation and Preliminary Results Technical Memorandum, Salmon Escapement Study, Study Plan Section 9.7. Susitna-Watana Hydroelectric Project, FERC Project No. 14241. September 2014. Anchorage, Alaska. http://www.susitna- watanahydro.org/wp-content/uploads/2014/09/09.07_ESCAPE_TM_Short- Version_new.pdf. AEA. 2014b. Initial Study Report. Fish and Aquatics Instream Flow Study, Study Plan Section 8.5, Part C. Susitna-Watana Hydroelectric Project, FERC Project No. 14241. June 2014. Anchorage, Alaska. 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Alaska Department of Fish and Game, Fishery Data Series No. 11-19 Anchorage. Yanusz, R.J., R.A. Merizon, T.M. Willette, D.G. Evans, and T.R. Spencer. 2011b. Inriver abundance and distribution of spawning Susitna River sockeye salmon Oncorhynchus nerka, 2008. Alaska Fishery Data Series No. 11-12. Department of Fish and Game, Divisions of Sport and Fish and Commercial Fisheries. Yanusz, R.J., P. Cleary, S. Ivey, J.W. Erickson, D.J. Reed, R.A. Neustel, and J. Bullock. 2013. Distribution of spawning Susitna River Chinook Oncorhynchus tshawytscha and pink salmon O. gorbuscha, 2012. Prepared by the Alaska Department of Fish and Game, Division of Sport Fish, for the Alaska Energy Authority, Susitna-Watana Hydroelectric Project. Zar. J.H. 1984. Biostatistical analysis. Prentice-Hall Inc., Englewood Cliffs, NJ. Zwane, E.N., and P.G.M. van der Heijden. 2003. Implementing the parametric bootstrap in capture–recapture models with continuous covariates. Statistics & Probability Letters, 65: 121–125. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 92 October 2015 9. TABLES STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 93 October 2015 Table 4.3-1. Aerial spawner surveys conducted in the Middle and Upper River by location and date, 2014. River Section Waterbody Miles Surveyed Middle River -Cheechako Creek Susitna 155.9 2.4 14-Jul 19-Jul 26-Jul 31-Jul 6-Aug 12-Aug 18-Aug Below Impediment 3 Chinook Creek Susitna 160.4 8.7 14-Jul 19-Jul 26-Jul 31-Jul 6-Aug 12-Aug 18-Aug Middle River -Devil Creek Susitna 164.8 2.5 14-Jul 19-Jul 26-Jul 31-Jul 6-Aug 12-Aug 18-Aug Above Impediment 3 Fog Creek Susitna 179.3 19.3 14-Jul 19-Jul 25-Jul 31-Jul 6-Aug 12-Aug 18-Aug Fog Creek Tributary L1 Fog 5.1 7.6 14-Jul 19-Jul 25-Jul 31-Jul 6-Aug 12-Aug 18-Aug Bear Creek Susitna 184.0 5.7 14-Jul 19-Jul 25-Jul 31-Jul 6-Aug 12-Aug 18-Aug Bear Creek Tributary R1 Bear 0.8 8.2 14-Jul 19-Jul 25-Jul 31-Jul 6-Aug 12-Aug 18-Aug Tsusena Creek Susitna 184.4 3.6 15-Jul 19-Jul 25-Jul 31-Jul 6-Aug 12-Aug 18-Aug Upper River -Deadman Creek Susitna 188.4 0.3 15-Jul 19-Jul 25-Jul 31-Jul 7-Aug 13-Aug 19-Aug Within Reservoir Watana Creek Susitna 196.9 21.3 15-Jul 20-Jul 25-Jul 1-Aug 7-Aug 13-Aug 19-Aug Watana Creek Tributary R5 Watana 8.6 8.6 15-Jul 20-Jul 25-Jul 1-Aug 7-Aug 13-Aug 19-Aug Kosina Creek Susitna 209.2 18.8 15-Jul 20-Jul 25-Jul 1-Aug 7-Aug 12-Aug 18-Aug Gilbert Creek Kosina 6.2 6 15-Jul NS2 NS2 1-Aug 7-Aug 13-Aug 19-Aug Tsisi Creek Kosina 7.3 6.4 15-Jul NS2 26-Jul 1-Aug 7-Aug 12-Aug 18-Aug Tsisi Lake 1 Tsisi 7.2 2.8 NS1 NS1 26-Jul 1-Aug 7-Aug 12-Aug NS1 Tsisi Lake 2 Tsisi 10.6 5.2 NS1 NS1 26-Jul 1-Aug 7-Aug 12-Aug 18-Aug Jay Creek Susitna 211.0 13.3 15-Jul 20-Jul 26-Jul 1-Aug 7-Aug 13-Aug 19-Aug Upper River -Goose Creek Susitna 232.9 11.2 15-Jul 20-Jul 26-Jul 1-Aug 7-Aug 13-Aug 19-Aug Above Reservoir Oshetna River Susitna 235.1 26.3 15-Jul 20-Jul 26-Jul 1-Aug NS2 13-Aug 19-Aug Black River Oshetna 6.2 15-Jul 20-Jul 26-Jul 1-Aug NS2 13-Aug 19-Aug 1 No survey - surveys targeting Sockeye Salmon began July 25-26. 2 No survey - high and/or turbid water prevented survey. Confluence Project River Mile Survey Dates STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 94 October 2015 Table 5.1-1. Number of adult salmon radio-tagged in the Susitna River Basin from 2012 to 2014, by species, fish size, and tagging location. Tagging Total Species (and Fish Size)Location 2012 2013 2014 (All Years) Chinook Salmon (Largea)Lower River 442 580 659 1,681 Yentna River 0 425 296 721 Middle River 352 536 590 1,478 Total (All Locations)794 1,541 1,545 3,880 Chinook Salmon (Smalla)Middle River 0 67 32 99 Chum Salmon Lower River 400 0 0 400 Middle River 279 201 200 680 Total (All Locations)679 268 232 1,179 Coho Salmon Lower River 399 596 658 1,653 Middle River 184 242 212 638 Total (All Locations)583 838 870 2,291 Pink Salmon Lower River 401 197 198 796 Middle River 230 200 201 631 Total (All Locations)631 397 399 1,427 Sockeye Salmon Lower River 100 0 0 100 Middle River 70 139 200 409 Total (All Locations)170 139 200 509 Total (All Species)Lower River 1,742 1,373 1,515 4,630 Yentna River 0 425 296 721 Middle River 1,115 1,385 1,435 3,935 Total (All Locations)2,857 3,183 3,246 9,286 a MEF ≥ 50 cm for large Chinook Salmon; MEF < 50 cm for small Chinook Salmon. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 95 October 2015 Table 5.2-1. Classifications for radio-tagged salmon in 2014, by species and release location. Chinook (< 50 cm) Sockeye Salmon Chum Salmon Classification (PRM) Lower River Middle River Yentna River Middle River Lower River Middle River Lower River Middle River Yentna River Middle River Middle River Tributary Destinations (total)574 438 227 20 155 164 565 173 48 76 141 Yentna River (32.4)113 0 219 0 20 1 46 1 43 0 0 Deshka River (44.9)136 0 4 0 24 0 89 0 3 1 0 Willow Creek (52.2)30 0 2 0 13 1 15 0 0 0 0 Little Willow Creek (55.6)22 0 1 0 5 0 15 0 0 0 0 Kashwitna River (64.7)16 1 0 0 5 1 9 1 0 0 0 Caswell Creek (67.4)0 0 0 0 1 0 15 0 0 0 0 Sheep Creek (70.1)6 0 0 0 1 0 4 1 0 0 0 Goose Creek (76.9)3 1 0 0 0 1 10 0 0 0 0 Montana Creek (80.9)16 5 0 0 7 5 11 2 0 0 3 Rabideux Creek (87.4)0 0 0 0 0 0 10 1 0 0 0 Sunshine Creek (88.1)1 0 0 0 0 0 26 4 0 0 0 Birch Creek (93.5)2 1 0 0 1 0 10 3 0 0 0 Trapper Creek (95.0)0 0 0 0 0 1 12 1 0 0 0 Talkeetna River (101.1)89 25 0 1 5 33 88 8 0 41 30 Chulitna River (102.4)109 15 1 0 69 11 172 4 2 25 10 Whiskers Creek (104.8)1 1 0 1 0 11 11 9 0 1 0 Chase Creek (110.5)0 1 0 0 0 0 6 6 0 0 0 Trib 113.7 (113.7)0 0 0 0 0 0 0 1 0 0 0 Slash Creek (114.8)0 0 0 0 0 0 0 2 0 0 0 Gash Creek (115.1)0 0 0 0 0 1 0 4 0 0 0 Lane Creek (117.1)0 3 0 2 0 0 1 4 0 0 0 5th of July Creek (127.3)0 0 0 0 0 1 0 0 0 0 0 4th of July Creek (134.3)0 8 0 0 2 26 0 4 0 0 3 Gold Creek (140.1)0 6 0 0 0 0 0 0 0 0 0 Indian River (141.8)17 182 0 8 2 61 14 98 0 2 49 Jack Long Creek (148.2)0 3 0 0 0 0 0 2 0 1 0 Portage Creek (152.3)12 183 0 8 0 10 1 17 0 5 46 Cheechako Creek (155.9)1 2 0 0 0 0 0 0 0 0 0 Kosina Creek (209.1)0 1 0 0 0 0 0 0 0 0 0 Pink SalmonChinook Salmon (≥ 50 cm)Coho Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 96 October 2015 Table 5.2-1. Continued. Chinook (< 50 cm) Sockeye Salmon Chum Salmon Classification (PRM) Lower River Middle River Yentna River Middle River Lower River Middle River Lower River Middle River Yentna River Middle River Middle River Mainstem Destinations (total)7 34 0 4 1 12 16 11 0 66 18 Mainstem Proper 2 4 0 0 1 0 0 2 0 0 2 Downstream of Lane (117.1)2 0 0 0 1 0 0 0 0 0 1 no prior spawn location 2 0 0 0 1 0 0 0 0 0 1 Upstream of Lane (117.1)0 4 0 0 0 0 0 2 0 0 1 no prior spawn location 0 3 0 0 0 0 0 2 0 0 1 was in Portage Creek 0 1 0 0 0 0 0 0 0 0 0 Tributary Mouths 2 22 0 3 0 7 3 3 0 0 5 Trapper Mouth (44.9)0 0 0 0 0 0 1 0 0 0 0 Montana Mouth (80.9)0 0 0 0 0 0 1 0 0 0 0 Rabideux Mouth (87.4)0 0 0 0 0 0 1 0 0 0 0 Talkeetna Mouth (101.0)1 0 0 0 0 0 0 0 0 0 0 Lane Mouth (117.1)0 1 0 0 0 0 0 0 0 0 0 no prior spawn location 0 0 0 0 0 0 0 0 0 0 0 was up Talkeetna River 0 1 0 0 0 0 0 0 0 0 0 5th of July Mouth (127.3)0 3 0 0 0 1 0 0 0 0 1 4th of July Mouth (134.3)0 3 0 0 0 3 0 0 0 0 1 no prior spawn location 0 1 0 0 0 2 0 0 0 0 1 was up 4th of July Creek 0 1 0 0 0 1 0 0 0 0 0 was up Indian River 0 1 0 0 0 0 0 0 0 0 0 Gold Mouth (140.1)0 1 0 0 0 0 0 0 0 0 1 Indian Mouth (141.8)0 10 0 3 0 3 0 2 0 0 1 no prior spawn location 0 8 0 1 0 3 0 2 0 0 1 was up Indian River 0 2 0 2 0 0 0 0 0 0 0 Portage Mouth (152.3)0 4 0 0 0 0 0 1 0 0 1 no prior spawn location 0 2 0 0 0 0 0 0 0 0 1 was up Portage Creek 0 2 0 0 0 0 0 1 0 0 0 Cheechako Mouth (155.9)1 0 0 0 0 0 0 0 0 0 0 Pink SalmonChinook Salmon (≥ 50 cm)Coho Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 97 October 2015 Table 5.2-1. Continued. Chinook (< 50 cm) Sockeye Salmon Chum Salmon Classification (PRM) Lower River Middle River Yentna River Middle River Lower River Middle River Lower River Middle River Yentna River Middle River Middle River Side Channels & Sloughs 3 8 0 1 0 5 13 6 0 66 11 Slough 8A (129.2)0 0 0 0 0 0 0 0 0 23 0 Slough 9 (131.4)0 0 0 0 0 0 0 0 0 10 1 Slough 11 (138.6)0 1 0 0 0 0 0 0 0 13 0 no prior spawn location 0 1 0 0 0 0 0 0 0 12 0 was up Portage Creek 0 0 0 0 0 0 0 0 0 1 0 Slough 21 (145.1)0 0 0 0 0 0 0 0 0 0 0 Other areas 3 7 0 1 0 5 13 6 0 20 10 no prior spawn location 3 6 0 1 0 5 11 5 0 20 9 was up Deshka River 0 0 0 0 0 0 2 0 0 0 0 was up Chulitna River 0 0 0 0 0 0 0 0 0 0 1 was up Indian River 0 1 0 0 0 0 0 0 0 0 0 was up Portage Creek 0 0 0 0 0 0 0 1 0 0 0 Other Classifications (total)75 118 68 8 43 25 59 46 12 58 41 Other Mainstem 31 60 4 3 22 17 49 20 0 49 31 Max Zone downstream of Lane 30 0 4 0 22 0 48 0 0 0 0 Max Zone upstream of Lane 1 60 0 3 0 17 1 20 0 49 31 Downstream Only 16 40 46 4 0 4 4 17 6 8 1 Near Release Site 13 17 10 1 20 3 2 8 1 1 7 No or Single Detections 15 1 8 0 1 1 4 1 5 0 2 Total Tags Released 656 590 295 32 199 201 640 230 60 200 200 Notes: Fish that were detected on several occasions within a limited area were classified with a 'Mainstem Destination' (either in side-channel/slough locations, in a tributary mouth, or in the mainstem proper). Some of the fish that showed the ‘Mainstem Destination’ detection pattern did so after entering a spawning tributary (those that had at least one live detection in the mainstem location and that spent less than 6 days in the tributary location are noted in the table – otherwise the mainstem detection was ignored and the fish was assigned to the tributary location). Tags that were recovered or returned were included in this table either under the 'Other Mainstem' classification (if the recovery date was outside of the range of probable spawning dates) or within the row that was associated with the recovery location (if recoveries were from within a tributary, or were in a possible mainstem spawning location). Pink SalmonChinook Salmon (≥ 50 cm)Coho Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 98 October 2015 Table 5.2-2. The proportions of radio-tagged salmon of known destination that were detected in the Middle and Upper rivers, and that subsequently returned downstream to enter a Lower River tributary, or that appeared to have a mainstem destination in the Lower River, 2014. Tagged in Lower River Classification Chinook Salmon Pink Salmon Coho Salmon Reached Lane Station 37 6 26 Unknown Destination 1 0 1 Known Classification (a)1 36 6 25 Mid/Upper-Susitna Tributary 30 4 15 Mid/Upper-Susitna Mainstem 1 0 0 Returned Downstream (b)1 5 2 10 Lane Creek 0 0 1 Chase Creek 0 0 2 Whiskers Creek 0 0 3 Chulitna River 3 1 3 Talkeetna River 1 1 0 Sunshine Creek 0 0 1 Montana Creek 1 0 0 Proportion Roaming (c)1 13.9%33.3%40.0% Tagged in Middle River Classification / Fate Chinook Salmon (≥ 50 cm) Chinook Salmon (< 50 cm) Pink Salmon Coho Salmon Sockeye Salmon Chum Salmon Tagged at Curry 590 32 201 230 200 200 Other Classification (from Table Table 5.2-1)118 8 25 46 58 41 Other Mainstem 60 3 17 20 49 31 Downstream Only 40 4 4 17 8 1 Near Release Site 17 1 3 8 1 7 No / Single Detections 1 0 1 1 0 2 Known Classification (a)1 472 24 176 184 142 159 Tributary above Curry 385 16 98 121 8 98 Susitna Mainstem above Curry 33 3 10 9 65 17 Returned Downstream (b)1 54 5 68 54 69 44 Mainstem Destination 1 1 2 2 1 1 Lane Creek 3 2 0 4 0 0 Gash Creek 0 0 1 4 0 0 Slash Creek 0 0 0 2 0 0 Trib 113.7 0 0 0 1 0 0 Chase Creek 1 0 0 6 0 0 Whiskers Creek 1 1 11 9 1 0 Chulitna River 15 0 11 4 25 10 Talkeetna River 25 1 33 8 41 30 Trapper Creek 0 0 1 1 0 0 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 99 October 2015 Table 5.2-2. Continued. Tagged in Middle River Classification / Fate Chinook Salmon (≥ 50 cm) Chinook Salmon (< 50 cm) Pink Salmon Coho Salmon Sockeye Salmon Chum Salmon Birch Creek 1 0 0 3 0 0 Rabideux Creek 0 0 0 1 0 0 Sunshine Creek 0 0 0 4 0 0 Montana Creek 5 0 5 2 0 3 Sheep Creek 0 0 0 1 0 0 Goose Creek 1 0 1 0 0 0 Kashwitna River 1 0 1 1 0 0 Willow Creek 0 0 1 0 0 0 Deshka River 0 0 0 0 1 0 Yentna River 0 0 1 1 0 0 Proportion Roaming (c)1 11.4%20.8%38.6%29.3%48.6%27.7% Notes: 1 c = b / a STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 100 October 2015 Table 5.2-3. Farthest upstream detection locations for radio-tagged fish that were eventually assigned to mainstem or tributary spawning locations downstream of Lane Station (top panel: fish released in the Lower River; bottom panel: fish released in the Middle River), 2014. Project river miles are shown in parentheses. Tagged in Lower River Farthest Upstream Location Chinook Salmon Pink Salmon Coho Salmon Lane Station (116.7)2 1 8 Near Curry (124.2)1 0 0 4th of July Ck. Mouth (134.3)0 0 1 Indian R. mouth (141.8)0 0 1 Powerline (145.7)1 0 0 Portage Ck. Mouth (152.3)0 1 0 Below Impediment 1 (155.2)1 0 0 Total number that reached Lane Station, then were assigned a downriver destination 5 2 10 Tagged in Middle River Farthest Upstream Location Chinook Salmon (≥ 50 cm) Chinook Salmon (< 50 cm) Pink Salmon Coho Salmon Sockeye Salmon Chum Salmon Near Curry (124.2)32 3 27 30 41 35 Gateway (130.1)6 2 9 6 5 2 4th of July mouth (134.3)1 0 0 1 0 0 Slough 11 mouth (138.7)0 0 0 1 0 0 Gold Creek mouth (140.1)0 0 0 0 1 0 Indian R. mouth (141.8)8 0 29 10 7 2 Powerline (145.7)1 0 0 1 3 1 Jack Long Ck. Mouth (148.2)0 0 0 0 3 1 Portage Ck. Mouth (152.3)4 0 3 5 6 3 Below Impediment 1 (155.2)2 0 0 0 1 0 Above Impediment 1 (155.2)0 0 0 0 2 0 Total number released in the Middle River, then were assigned a downriver destination 54 5 68 54 69 44 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 101 October 2015 Table 5.3-1. Number of salmon radio-tagged in the Lower and Middle rivers, and the number of radio- tagged salmon that were detected at or above the Gateway Station, above each impediment, and above the proposed dam site, 2014. Radio At or Above Above Above Above Above Species Tags Gateway Impediment 1 Impediment 2 Impediment 3 Dam Site Tag Site Applied (PRM 130.1)(PRM 155.2)(PRM 160.2)(PRM 164.8)(PRM 187.1) Chinook Salmon (Large) Tagged in Lower River 659 34 2 1 0 0 Tagged in Middle River 590 491 11 8 2 1 Total Tagged 1,249 525 13 9 2 1 Chinook Salmon (Small) Tagged in Middle River 32 24 0 0 0 0 Chum Salmon Tagged in Middle River 200 154 0 0 0 0 Coho Salmon Tagged in Lower River 640 17 0 0 0 0 Tagged in Middle River 230 170 0 0 0 0 Total Tagged 870 187 0 0 0 0 Pink Salmon Tagged in Lower River 198 5 0 0 0 0 Tagged in Middle River 201 164 0 0 0 0 Total Tagged 399 169 0 0 0 0 Sockeye Salmon Tagged in Middle River 200 146 3 0 0 0 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 102 October 2015 Table 5.3-2. Details of the radio-tagged salmon that approached or passed the Middle River impediments, 2014. Chinook Salmon (≥ 50 cm) that Passed Impediment 3 (PRM 164.4) Tag Number Capture/ Release Site Capture Date METF Length (cm)Sex First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Comments 537 Curry, Site Three 4 Jul 80 Male 20 Jul 20 Jul 4 Aug Just above I3, then mort DS 787 Curry, Site Two 11 Jul 78 Undetermined 20 Jul 20 Jul 30 Jul Kosina (8/2-8/7), Oshetna (8/9), then Kosina (8/12-18), drfited to below Fog Ck. Chinook Salmon (≥ 50 cm) that Passed Impediment 2 (PRM 160.2) but not Impediment 3 (PRM 164.4) Tag Number Capture/ Release Site Capture Date METF Length (cm)Sex First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Comments 17 Curry, Site One 14 Jun 70 Undetermined 30 Jun 30 Jun -Below I3, then Cheechako (7/10) then Portage (7/14- 8/6) then mort DS 139 Curry, Site One 21 Jun 61 Undetermined 24 Jul 28 Jul -Cheechako (7/25-26) then mort near Chinook Creek 222 Curry, Site Two 24 Jun 75 Undetermined 6 Jul 18 Jul -Below I3, then mort DS 516 Curry, Site One 4 Jul 87 Undetermined 1 Aug 1 Aug -Cheechako to Chinook mouths, then Cheechako (8/9) then out, mort at mouth 882 Curry, Site Three 16 Jul 51 Undetermined 25 Jul 1 Aug -Chinook mouth then Cheechako (8/3-9) then mort DS 903 Curry, Site Three 17 Jul 78 Undetermined 23 Jul 24 Jul -Below I3, mort between Chinook and I3 5531 Lower River, gill net 12 Jun 93 n/a 18 Jul 18 Jul -Below I3, then in Cheechako (8/12) and at mouth (8/15- 9/2) Chinook Salmon (≥ 50 cm) that Passed Impediment 1 (PRM 155.2) but not Impediment 2 (PRM 160.2) Tag Number Capture/ Release Site Capture Date METF Length (cm)Sex First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Comments 221 Curry, Site One 24 Jun 92 Undetermined 20 Jul --Portage (7/10), just Above I1, then Below I1, drifted as mort DS 828 Curry, Site Three 13 Jul 55 Undetermined 18 Jul --Cheechako Stn, then Portage 868 Curry, Site Three 15 Jul 94 Male 23 Jul --Cheechako (7/31-8/1 and 8/6-8/12), mouth (to 8/18) then drifted DS to below Portage 5702 Lower River, gill net 23 May 91 n/a 1 Jul --0.75 mi above Cheechako Stn, then in Cheechako STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 103 October 2015 Table 5.3-2. Continued. Chinook Salmon (≥ 50 cm) that Approached Impediment 1 (PRM 155.2) but did not Pass Tag Number Capture/ Release Site Capture Date METF Length (cm)Sex First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Comments 23 Curry, Site Two 14 Jun 63 Undetermined ---Below I1, Portage (7/25-8/4), then mort DS 33 Curry, Site Two 15 Jun 63 Undetermined ---Below I1, then Talkeetna 40 Curry, Site Two 16 Jun 68 Undetermined ---Below I1, then Portage 91 Curry, Site Three 19 Jun 92 Undetermined ---Below I1, then Portage 103 Curry, Site One 20 Jun 81 Undetermined ---mort Below I1 108 Curry, Site Two 20 Jun 99 Undetermined ---Below I1, Portage (7/22-23) then DS 111 Curry, Site Three 20 Jun 97 Undetermined ---Portage (7/4-7/5), Below I1, Portage (7/19-onwards) 166 Curry, Site One 22 Jun 63 Undetermined --- Below I1, Portage (7/14), Portage mouth (7/22-8/4), mort DS 198 Curry, Site One 23 Jun 78 Undetermined ---Below I1, then Talkeetna 237 Curry, Site One 25 Jun 93 Male ---Below I1, Indian (7/22-8/6) then DS 239 Curry, Site One 25 Jun 87 Female ---Below I1, then Portage 244 Curry, Site Two 25 Jun 84 Undetermined --- Below I1, then Portage (mid-Aug onward, incl mort 8/20) 264 Curry, Site One 28 Jun 78 Undetermined ---Below I1, then Gold Creek 300 Curry, Site One 29 Jun 66 Undetermined ---Portage mouth , Below I1, then up Portage 359 Curry, Site Three 30 Jun 59 Undetermined ---Below I1, then mort DS 562 Curry, Site One 5 Jul 79 Undetermined ---Below I1, then Portage 611 Curry, Site Three 5 Jul 91 Undetermined ---Below I1, then Portage 621 Curry, Site One 6 Jul 87 Undetermined ---Below I1, then Portage 668 Curry, Site Three 6 Jul 80 Undetermined ---Below I1, then Portage 716 Curry, Site One 8 Jul 95 Undetermined ---Below I1, then mort DS 818 Curry, Site Two 13 Jul 64 Undetermined ---Below I1, then Indian (7/26-8/5) then mort DS of mouth 5242 Lower River, East Bank 4 Jun 75.5 n/a ---Chulitna, Below I1, then Chulitna 5255 Lower River, East Bank 7 Jun 83 n/a ---Deshka, Below I1, then Portage 5384 Lower River, West Bank 17 Jun 73.5 n/a ---Below I1, then mort DS 5408 Lower River, gill net 31 May 93 n/a ---Below I1, then Portage STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 104 October 2015 Table 5.3-2. Continued. Chinook Salmon (< 50 cm) that Approached Impediment 1 (PRM 155.2) but did not Pass Tag Number Capture/ Release Site Capture Date METF Length (cm)Sex First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Comments 574 Curry, Site One 5 Jul 41 Undetermined ---Below I1, Portage (7/25-8/4), then Indian (8/9), then back to Portage (8/15-onwards) Sockeye Salmon That Passed Impediment 1 (PRM 155.2) but not Impediment 2 (PRM 160.2) Tag Number Capture/ Release Site Capture Date METF Length (cm)Sex First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Comments 785 Curry, Site Two 11 Jul 48 Undetermined 27 Jul -- Cheechako Stn, dropback, held below I1, moved into Jack Long (9/5), then mort DS 955 Curry, Site Three 19 Jul 49 Undetermined 8 Sep -- Detected above I1 for a single survey (9/8), then moved into Chulitna 2214 Curry, Site Three 8 Aug 45 Undetermined 8 Sep -- Chulitna (8/13-19), moved above I1 for a single survey (9/8), then wandered Sockeye Salmon That Approached Impediment 1 (PRM 155.2) but did not Pass Tag Number Capture/ Release Site Capture Date METF Length (cm)Sex First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Comments 647 Curry, Site Two 6 Jul 60 Undetermined ---Below I1, then mort DS 837 Curry, Site Two 14 Jul 47 Undetermined ---Below I1, then mort DS 1053 Curry, Site One 23 Jul 46 Undetermined --- Portage (1 survey), Below I1, then Side-channel outside Slough 21 1260 Curry, Site Three 26 Jul 48 Undetermined --- Below I1 four times interspersed by wandering, then mort DS 1293 Curry, Site Two 27 Jul 55 Undetermined ---Below I1, then Talkeetna 1346 Curry, Site Two 28 Jul 45 Undetermined ---Below I1 , then mort DS 1404 Curry, Site Two 29 Jul 43 Undetermined ---Below I1 , then mort DS 1851 Curry, Site Two 4 Aug 54 Female ---Below I1 , then mort DS 2076 Curry, Site Two 7 Aug 45 Undetermined ---Below I1, then into 4th of July Slough 2156 Curry, Site One 8 Aug 42 Undetermined ---Below I1 , then mort DS STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 105 October 2015 Table 5.3-2. Continued. Sockeye Salmon That Approached Impediment 1 (PRM 155.2) but did not Pass Tag Number Capture/ Release Site Capture Date METF Length (cm)Sex First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Comments 2157 Curry, Site One 8 Aug 45 Undetermined ---Below I1, Portage, Below I1, Side Channel 21 2606 Curry, Site One 13 Aug 51 Undetermined --- Below I1 three times interspersed by wandering, then mort DS 2770 Curry, Site One 15 Aug 53 Male --- Below I1 twice interspersed by wandering, the Side Channel 21 Notes: Fish characteristics include 'tag numbers' (unique numbers assigned to each individual radio-tagged fish), capture and release site, capture date, METF (mid-eye to fork length, in cm) and sex. Tracking details include the date of first detections above each impediment, and a comment about the general movments of the fish. Top panel: Chinook salmon (≥ 50 cm) that passed Impediment 3. Second panel: Chinook salmon (≥ 50 cm) that passed Impediment 2, but not Impediment 3. Third panel: Chinook salmon (≥ 50 cm) that passed Impediment 1, but not Impediment 2. Fouth panel: Chinook salmon (≥ 50 cm) that approached within 1 km of Impediment 1, but did not pass. Fifth panel: Chinook salmon (< 50 cm) that approached within 1 km of Impediment 1, but did not pass. Fifth panel: Chinook salmon (< 50 cm) that approached within 1 km of Impediment 1, but did not pass. Sixth panel: Sockeye salmon that passed Impediment 1, but not Impediment 2. Seventh panel: Sockeye salmon that approached within 1 km of Impediment 1, but did not pass. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 106 October 2015 Table 5.3-3. Destinations of radio-tagged salmon that passed each Middle River impediment, 2014. Sockeye Salmon Passed I1 but not I2 Passed I2 but not I3 Passed I3 Total Passed I1 but not I2 Grand Total Classification Tributary Destinations Chulitna River 2 2 Jack Long Creek 1 1 Portage Creek 1 1 2 2 Cheechako Creek 2 1 3 3 Kosina Creek 1 1 1 Mainstem Destinations Mouth of Cheechako 1 1 1 Unknown Destination 1 4 1 6 6 Total 4 7 2 13 3 16 Downstream from Impediment Number 1 3 0 4 3 7 Percent 33%100%0%57%100%70% Notes: An “I” refers to “impediment.” Shaded cells refer to areas that are located downstream of the impediment in question. Chinook Salmon (≥ 50 cm) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 107 October 2015 Table 5.3-4. Dates on which radio-tagged fish were first detected upstream of Impediment 3 (2012-2014), with corresponding flows as measured at Tsusena and Gold creeks. Flow at I-1 Passage (cfs) Flow at I-2 Passage (cfs) Flow at I-3 Passage (cfs) Flow at I-1 Passage (cfs) Flow at I-2 Passage (cfs) Flow at I-3 Passage (cfs) Chinook Salmon (≥ 50 cm) that Passed Impediment 3 in 2012 27 15 Jul 2012 16 Jul 2012 18 Jul 2012 15,800 16,500 15,200 18,608 19,252 17,774 52 7 Jul 2012 7 Jul 2012 17 Jul 2012 17,800 17,800 15,800 21,302 21,302 19,042 94 8 Jul 2012 12 Jul 2012 17 Jul 2012 20,800 16,400 15,800 26,550 20,060 19,042 104 18 Jul 2012 18 Jul 2012 20 Jul 2012 15,200 15,200 15,000 17,774 17,774 17,320 113 15 Jul 2012 15 Jul 2012 19 Jul 2012 15,800 15,800 14,900 18,608 18,608 17,407 219 15 Jul 2012 16 Jul 2012 19 Jul 2012 15,800 16,500 14,900 18,608 19,252 17,407 246 13 Jul 2012 14 Jul 2012 20 Jul 2012 15,600 15,500 15,000 18,755 18,275 17,320 257 15 Jul 2012 16 Jul 2012 20 Jul 2012 15,800 16,500 15,000 18,608 19,252 17,320 266 15 Jul 2012 16 Jul 2012 19 Jul 2012 15,800 16,500 14,900 18,608 19,252 17,407 359 12 Jul 2012 12 Jul 2012 17 Jul 2012 16,400 16,400 15,800 20,060 20,060 19,042 5005 17 Jul 2012 17 Jul 2012 17 Jul 2012 15,800 15,800 15,800 19,042 19,042 19,042 5019 9 Jul 2012 17 Jul 2012 18 Jul 2012 24,600 15,800 15,200 31,067 19,042 17,774 Chinook Salmon (≥ 50 cm) that Passed Impediment 3 in 2013 241 13 Jul 2013 14 Jul 2013 16 Jul 2013 14,383 15,410 16,672 16,178 18,233 18,632 272 13 Jul 2013 14 Jul 2013 30 Jul 2013 14,383 15,410 18,848 16,178 18,233 19,657 395 11 Jul 2013 12 Jul 2013 13 Jul 2013 16,876 15,058 14,383 20,272 17,985 16,178 Chinook Salmon (≥ 50 cm) that Passed Impediment 3 in 2014 537 20 Jul 2014 20 Jul 2014 4 Aug 2014 21,400 21,400 16,400 25,900 25,900 19,200 787 20 Jul 2014 20 Jul 2014 30 Jul 2014 21,400 21,400 15,900 25,900 25,900 19,400 Gold CreekTsusena CreekFirst Detection Above I-3 First Detection Above I-2 First Detection Above I-1 Tag Number STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 108 October 2015 Table 5.3-5. Aerial Chinook Salmon spawning escapement surveys. Number of flights, and date and magnitude of peak counts per stream and survey year. The number of radio-tagged Chinook Salmon that were classified to each stream (see Table 5.2-1) is included for 2012-2014. Stream # FlightsDate of Peak CountPeak Count# FlightsDate of Peak CountPeak Count# FlightsDate of Peak CountPeak Count# FlightsDate of Peak CountPeak CountCheechako Cr 9 6 Aug 16 2 1 Aug 25 7 1 Aug 29 11 24 Jul 18 Chinook Cr 5 6 Aug 5 2 1 Aug 8 7 1 Aug 15 11 23 Aug 1 Devil Cr 5 na 0 1 1 Aug 1 6 na 0 11 na 0 Fog Cr 0 0 4 21 Jul 2 3 na 0 Bear Cr 0 0 4 na 0 3 na 0 Tsusena Cr 0 0 4 na 0 3 na 0 Deadman Cr 0 0 3 na 0 0 Watana Cr 0 0 2 na 0 0 Kosina Cr 0 0 0 0 Jay Cr 0 0 0 0 Goose Cr 0 0 0 0 Oshetna Cr 0 0 0 0 19831982 19851984 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 109 October 2015 Table 5.3-5. Continued. Stream # FlightsDate of Peak CountPeak CountClassified Radio-tagged Fish# FlightsDate of Peak CountPeak CountClassified Radio-tagged Fish# FlightsDate of Peak CountPeak CountClassified Radio-tagged FishCheechako Cr 4 30 Jul 5 6 5 26 Jul 40 6 6 19 Jul 16 3 Chinook Cr 4 5 Aug 5 3 5 26 Jul 2 1 6 19/25 Jul 5 0 Devil Cr 4 5 Aug 7 1 5 26 Jul 25 1 6 6 Aug 10 0 Fog Cr 4 30 Jul 1 0 5 9/15 Aug 2 0 6 31 Jul 3 0 Bear Cr 4 na 0 0 5 na 0 0 6 na 0 0 Tsusena Cr 4 na 0 0 5 9 Aug 4 1 6 na 0 0 Deadman Cr 4 na 0 0 5 na 0 0 6 na 0 0 Watana Cr 4 na 0 0 5 na 0 0 6 na 0 0 Kosina Cr 4 5 Aug 16 6 5 26 Jul 3 0 6 na 0 1 Jay Cr 4 na 0 0 5 na 0 0 6 na 0 0 Goose Cr 4 na 0 0 5 na 0 0 6 na 0 0 Oshetna Cr 4 na 0 0 5 na 0 0 5 na 0 0 2012 2013 2014 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 110 October 2015 Table 5.4-1. Survey effort and observations using DIDSON to identify Chinook Salmon spawning behavior in turbid water, 2014. Date Sample Location Latitiude Longitude DIDSON Used Chinook Observed Spawning Observed Redds Observed Comments 19-Jul Gateway Slough 62.67643 -149.89302 Yes No -- 19-Jul Mainstem gravel bar, d/s PRM 133 62.70674 -149.84082 No No -- 19-Jul 4th of July Slough (60 m u/s of outlet)62.71587 -149.80301 Yes No -- 19-Jul Mainstem side channel, PRM 135.5 62.72485 -149.75978 No No --Inaccessible by boat 19-Jul Slough 10 62.73838 -149.74134 No No --No potential sampling sites 19-Jul Slough 11 62.74281 -149.72163 No No --Inaccessible by boat 20-Jul Slough ?1 "Hidden Slough"62.58162 -150.04994 Yes No -- 20-Jul Side channel, near PRM 117 62.53213 -150.10708 Yes No --Entrained air and river velocity precluded usable sonar imagery 20-Jul Mainstem d/s 4th of July Creek mouth 62.71481 -149.80823 Yes Yes No No Individuals observed milling/holding 20-Jul 4th of July Slough (30 m u/s of outlet)62.71558 -149.80345 No No -- 20-Jul 4th of July Slough (100 m u/s outlet)62.71701 -149.80208 No No --Large cobble substrate 21-Jul Portage Creek mouth, river right 62.83034 -149.38153 No No -- 21-Jul Mainstem d/s Portage Cr. mouth, river right 62.83035 -149.38403 Yes Yes No No Individuals observed milling/holding 21-Jul Mainstem d/s Portage Cr. mouth, river right 62.83116 -149.38715 No No -- 21-Jul Mainstem u/s Jack Long Cr. mouth, river left 62.82270 -149.49220 No No --Sand substrate 21-Jul Mainstem u/s Jack Long Cr. mouth, river left 62.82264 -149.49434 No No --Large cobble substrate 21-Jul Mainstem d/s Jack Long Cr. mouth, river left 62.82243 -149.49821 Yes Yes Yes Yes Individual observed guarding and holding 21-Jul Mainstem d/s Jack Long Cr. mouth, river left 62.82150 -149.50507 Yes No -- 21-Jul Mainstem d/s Gold Cr. mouth, river left 62.76779 -149.69141 No No -- 21-Jul Mainstem d/s Sherman Cr. mouth, river left 62.7131 -149.81103 No No -- 21-Jul Mainstem d/s Skull Cr. mouth, river left 62.67699 -149.86920 No No -- 22-Jul Side channel entrance u/s Indian R., river right 62.79191 -149.62464 No No --Areas of upwelling 22-Jul Side channel exit u/s Indian R., river right 62.78956 -149.63977 No No -- 22-Jul Mainstem below side channel, river right 62.78861 -149.64438 No No --Sand substrate 22-Jul Mainstem at Beaver impoundment exit, river right 62.78752 -149.65044 No No -- STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 111 October 2015 Table 5.4-1. Continued. Date Sample Location Latitiude Longitude DIDSON Used Chinook Observed Spawning Observed Redds Observed Comments 22-Jul Mainstem d/s Indian R. delta (10 m), river right 62.78514 -149.65891 Yes Yes No No Individuals observed milling/holding 22-Jul Mainstem d/s Indian R. delta, over flow channel, river right 62.78413 -149.66248 No No -- 22-Jul Mainstem d/s Indian R. slough entrance 62.78296 -149.66805 Yes Yes No No Individuals observed milling/holding 22-Jul Mainstem d/s Indian R., river right 62.78145 -149.67789 No No -- 22-Jul Mainstem d/s Indian R. slough exit 62.77943 -149.68706 Yes No -- 23-Jul Slough u/s Gold Cr., river left 62.77146 -149.68672 No No --Sand and large cobble substrate 23-Jul Mainstem d/s Gold Cr., river right 62.76829 -149.69449 No No -- 23-Jul Mainstem d/s Gold Cr., river right 62.76650 -149.71121 Yes Yes No No Traveling u/s observed 23-Jul Mainstem channel d/s Curry unnamed tributary delta, river right 62.59989 -150.03344 No No -- 25-Jul Confirmation: d/s Portage Cr Mouth 62.83044 -149.38871 Yes Yes No No Individuals observed milling/holding 25-Jul Confirmation: d/s Jack Long Cr Mouth 62.82243 -149.49821 Yes Yes No No Individual observed milling/holding 25-Jul Confirmation: d/s 4th of July Cr Mouth 62.71475 -149.80908 Yes Yes No Yes Individual observed milling/holding STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 112 October 2015 Table 6.2-1. Confirmed spawning salmon in slough habitats of the Middle River from 1981 to 1985, and confirmed spawning locations in 2012, 2013, and 2014. Project River Slough Mile 2012 2013 2014 2012 2013 2014 2012 2013 2014 2012 2013 2014 1 103.2 X X 2 104.3 X X X 3B 105.5 X X X X X 3A 105.7 X X X 4 108.9 5 111.3 X X X 6 111.9 6A 115.9 X X X 7 116.6 8 117.2 X X X Bushrod 117.8 X X 8D 125.2 X X 8C 125.2 X X X 8B 125.7 X X X Moose 126.7 X X X A'128.0 X X A 128.3 X X X 8A 129.5 X X X X X X X X X X X B 130.0 X X X 9 131.7 X X X X X X X X 9B 132.5 X X 9A 136.3 X X X X X X 10 137.1 X X X X X 11 138.7 X X X X X X X X X 12 138.2 13 138.9 X X X 14 139.4 X 15 140.6 X X X 16 141.1 X X Confirmed Spawning Coho Salmon (1981-1985) Confirmed Spawning Chum Salmon Coho Salmon Pink Salmon Sockeye Salmon Sockeye SalmonChum Salmon Pink Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 113 October 2015 Table 6.2-1. Continued. Project River Slough Mile 2012 2013 2014 2012 2013 2014 2012 2013 2014 2012 2013 2014 17 142.3 X X X 18 142.5 X - 19 143.2 X X X X X X 20 143.6 X X X 21 145.2 X X X X X X X X X 22 147.6 X X X X X 21A 148.6 X X Note: Historic data (1981 - 1985) was synthesized from Barrett et al. (1985) and Thompson et al. (1986) Results from 2012 - 2014 also include some confirmed spawning locations provided by R2USA (1981-1985) Confirmed Spawning Chum Salmon Coho Salmon Pink Salmon Sockeye Salmon Chum Salmon Coho Salmon Pink Salmon Sockeye Salmon Confirmed Spawning STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 114 October 2015 10. FIGURES STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 115 October 2015 Figure 3-1. Susitna River watershed showing fish capture sites (fishwheels) and the locations of fixed-station telemetry receiver sites, 2014. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 116 October 2015 Figure 3-1 (cont). Susitna River watershed showing fish capture sites (fishwheels) and the locations of fixed-station telemetry receiver sites, 2014. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 117 October 2015 Figure 4.1-1. Middle River Segment showing sites for fish capture (Site 1, PRM 124.1; Site 2, PRM 123.0; and Site 3, PRM 126.0), sonar (ARIS; PRM 124.0), Curry camp (PRM 124.2), and the Lane Creek (PRM 116.7) and ‘Gateway’ (PRM 130.1) fixed-station receiver sites, 2014. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 118 October 2015 Figure 4.3-1. Extent of aerial spawner surveys in the Indian River and tributaries in and above Devils Canyon, 2014. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 119 October 2015 Figure 5.1-1. Daily discharge of the Susitna River at the Gold Creek gauge from April to November in 2012, 2013, and 2014. Historical (1949-2011) median, and 10th and 90th percentile discharges are shown for reference. Source: USGS National Water Information System (http://waterdata.usgs.gov/nwis). Figure 5.1-2. Daily discharge of the Susitna River at the Tsusena Creek gauge from April to November in 2012, 2013, and 2014. Source: USGS National Water Information System (http://waterdata.usgs.gov/nwis). 0 15,000 30,000 45,000 60,000 75,000 90,000 4-14-114-215-15-115-215-316-106-206-307-107-207-308-98-198-299-89-189-2810-810-1810-2811-711-1711-27Discharge (cfs)Date (m/d) 2014 2013 2012 10/90th Percentiles (1949-2011)Median (1949-2011) Susitna at Gold Creek 0 15,000 30,000 45,000 60,000 75,000 4-14-114-215-15-115-215-316-106-206-307-107-207-308-98-198-299-89-189-2810-810-1810-2811-711-1711-27Discharge (cfs)Date (m/d) 2014 2013 2012 Susitna at Tsusena Creek STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 120 October 2015 Figure 5.1-3. Median travel speeds of radio-tagged fish in four major river reaches, by species. Error bars represent 95% confidence in the median value (generated using the method recommended in Zar 1984). Statistical comparisons (see text) were done using Kruskal-Wallis tests; overlapping error bars do not preclude statistical significance. 0 5 10 15 20 25 30 35 Chinook salmon Chum salmon Coho salmon Pink salmon Sockeye salmon Travel Speed (km/d) Gateway to Indian Curry to Gateway Sunshine to Lane Deshka to Sunshine STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 121 October 2015 Figure 5.2-1. Classifications for radio-tagged salmon released in the Lower River (left panels) or Middle River (right panels), by species/life history stage, 2014. Top panels: Relative use of tributary and mainstem destinations, shown as a percentage of the total number of fish that were classified to a destination. Middle Panels: Relative use of mainstem habitats (side-channel/slough locations, tributary mouths, and the mainstem proper), shown as a percentage of the total number of fish that were classified with a 'Mainstem Destination'. Bottom Panel: Relative use of various sloughs and side-channel locations, shown as a percentage of the total number of fish that were classified with a 'Side-channel/Slough Mainstem Destination'. See text and Table 5.2-1 for more detailed classifications. 0% 20% 40% 60% 80% 100% Chinook Salmon Pink Salmon Coho SalmonRelative FrequencyTributary Mainstem General Classification Releases from Lower River 0% 20% 40% 60% 80% 100% Chinook Salmon Pink Salmon Coho SalmonRelative FrequencyMainstem Proper Tributary Mouths Side-channel / Sloughs Mainstem Use Releases from Lower River 0% 20% 40% 60% 80% 100% Chinook (≥ 50 cm) Chinook (< 50 cm) Pink Salmon Coho Salmon Sockeye Salmon Chum Salmon General Classification Releases from Middle River 0% 20% 40% 60% 80% 100% Chinook (≥ 50 cm) Chinook (< 50 cm) Pink Salmon Coho Salmon Sockeye Salmon Chum Salmon Mainstem Use Releases from Middle River 0% 20% 40% 60% 80% 100% Chinook (≥ 50 cm) Chinook (< 50 cm) Pink Salmon Coho Salmon Sockeye Salmon Chum SalmonRelative FrequencySlough 8A Slough 9 Slough 11 Slough 21 Side-channels Side -channel / Slough Use -Releases from Middle River STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 122 October 2015 Figure 5.2-2. Relative frequencies of tributary use by radio-tagged salmon released in the Lower River, by species, 2014. Shown as a percentage of all fish classified to a tributary destination. Shown as a percentage of all fish classified to a tributary destination. Relative Frequency0% 10% 20% 30% 40% 50%Pink Salmon 0% 10% 20% 30% 40% 50%YentnaDeshkaWillowLittle WillowKashwitnaCaswellGooseSheepMontanaSunshineBirchRabideuxTrapperTalkeetnaChulitnaWhiskersChaseLane4th of JulyIndianPortageCheechakoKosinaOtherStock Coho Salmon 0% 10% 20% 30% 40% 50% Chinook Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 123 October 2015 Figure 5.2-3. Relative frequencies of tributary use by radio-tagged salmon released in the Middle River, by species, 2014.hown as a percentage of all fish classified to a tributary destination. Relative Frequency0% 15% 30% 45% 60%Chinook Salmon (≥ 50 cm) 0% 15% 30% 45% 60%Coho Salmon 0% 15% 30% 45% 60%Chinook Salmon (< 50 cm) 0% 15% 30% 45% 60%Pink Salmon 0% 15% 30% 45% 60%MontanaTalkeetnaChulitnaOther Lower SuWhiskersChaseTrib 113.7SlashGashLane5th of July4th of JulyGoldIndianJack LongPortageCheechakoKosinaStock Chum Salmon 0% 15% 30% 45% 60%Sockeye Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 124 October 2015 Figure 5.3-1. Daily numbers of radio-tagged large Chinook Salmon that approached and passed each of the three Middle River impediments in 2014. Orange bars: fish that approached but did not pass. Blue bars: fish that approached and successfully passed. Figures show the date of first detection above the impediment (blue) or the date of first detection below the impediment (orange). Also shown is the average daily flow of the Susitna River as measured at the Tsusena Creek gauge. An “I” refers to “Impediment.” 0 1 2 3 4 5 6 7 8 0 5,000 10,000 15,000 20,000 25,000 30,000 35,000 40,000 6/10 6/13 6/16 6/19 6/22 6/25 6/28 7/1 7/4 7/7 7/10 7/13 7/16 7/19 7/22 7/25 7/28 7/31 8/3 8/6 8/9 8/12 8/15 8/18 8/21 8/24 8/27 8/30 9/2 9/5 9/8 9/11Number of Fish at I1Tsusena Discharge (cfs)Date (m/d) Chinook Salmon (≥ 50 cm) Impediment 1 (PRM 155.2) 0 1 2 3 4 5 6 7 8 0 5,000 10,000 15,000 20,000 25,000 30,000 35,000 40,000 6/10 6/13 6/16 6/19 6/22 6/25 6/28 7/1 7/4 7/7 7/10 7/13 7/16 7/19 7/22 7/25 7/28 7/31 8/3 8/6 8/9 8/12 8/15 8/18 8/21 8/24 8/27 8/30 9/2 9/5 9/8 9/11Number of Fish at I3Tsusena Discharge (cfs)Passed Approached (did not pass) Chinook Salmon (≥ 50 cm) Impediment 3 (PRM 164.4) 0 1 2 3 4 5 6 7 8 0 5,000 10,000 15,000 20,000 25,000 30,000 35,000 40,000 6/10 6/13 6/16 6/19 6/22 6/25 6/28 7/1 7/4 7/7 7/10 7/13 7/16 7/19 7/22 7/25 7/28 7/31 8/3 8/6 8/9 8/12 8/15 8/18 8/21 8/24 8/27 8/30 9/2 9/5 9/8 9/11Number of Fish at I2Tsusena Discharge (cfs)Chinook Salmon (≥ 50 cm) Impediment 2 (PRM 160.2) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 125 October 2015 Figure 5.3-2. Daily numbers of radio-tagged small Chinook (top panel) and Sockeye (bottom panel) salmon that approached and passed Middle River Impediment 1 in 2014. Orange bars: fish that approached but did not pass. Blue bars: fish that approached and successfully passed. Figures show the date of first detection above the impediment (blue) or the date of first detection below the impediment (orange). Also shown is the average daily flow of the Susitna River as measured at the Tsusena Creek gauge. An “I” refers to “Impediment.” No Chinook Salmon measuring less than 50 cm (19.7 in) METF or Sockeye Salmon approached or passed Impediment 2 or Impediment 3. 0 1 2 3 4 5 6 7 8 0 5,000 10,000 15,000 20,000 25,000 30,000 35,000 40,000 6/10 6/13 6/16 6/19 6/22 6/25 6/28 7/1 7/4 7/7 7/10 7/13 7/16 7/19 7/22 7/25 7/28 7/31 8/3 8/6 8/9 8/12 8/15 8/18 8/21 8/24 8/27 8/30 9/2 9/5 9/8 9/11Number of Fish at I1Tsusena Discharge (cfs)Date Approached (did not pass) Passed Sockeye Salmon Impediment 1 (PRM 155.2) 0 1 2 3 4 5 6 7 8 0 5,000 10,000 15,000 20,000 25,000 30,000 35,000 40,000 6/10 6/13 6/16 6/19 6/22 6/25 6/28 7/1 7/4 7/7 7/10 7/13 7/16 7/19 7/22 7/25 7/28 7/31 8/3 8/6 8/9 8/12 8/15 8/18 8/21 8/24 8/27 8/30 9/2 9/5 9/8 9/11Number of Fish at I1Tsusena Discharge (cfs)Date Approached (did not pass) Passed Chinook Salmon (< 50 cm) Impediment 1 (PRM 155.2) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 126 October 2015 Figure 5.3-3. Daily number of radio-tagged Chinook Salmon that held below Impediment 3 in 2014. Each of the two fish is shown using a unique color. Passage dates can be read by noting the date after which each of the tags disappears from the chart. Also shown is the average daily flow of the Susitna River as measured at the Tsusena Creek gauge. 0 5,000 10,000 15,000 20,000 25,000 30,000 35,000 40,000 45,000 0 1 2 3 6/21 6/23 6/25 6/27 6/29 7/1 7/3 7/5 7/7 7/9 7/11 7/13 7/15 7/17 7/19 7/21 7/23 7/25 7/27 7/29 7/31 8/2 8/4 8/6Tsusena Discharge (cfs)Radio-tagged Large Chinook SalmonDate Tag 787 Tag 537 Flow Chinook Salmon (≥ 50 cm)that Passed Impediment 3 (PRM 164.4) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 127 October 2015 Figure 5.3-4. Flows (measured at Gold Creek) in 2012, 2013 and 2014, along with median (solid black line), and the 10th and 90th percentile (dotted lines) historical flows. Plotted along the flow curves are passage events (yellow dots), where the dot size is scaled to indicate the number of fish that passed. Passage events and corresponding discharge are presented in Table 5.3-4. 10,000 15,000 20,000 25,000 30,000 35,000 40,000 45,000 50,000 55,000 1-Jun8-Jun15-Jun22-Jun29-Jun6-Jul13-Jul20-Jul27-Jul3-Aug10-Aug17-Aug24-Aug31-AugGold Creek Discharge (cfs)Chinook Salmon Passage at Devils Canyon Impediment 1 2012 2013 2014 10,000 15,000 20,000 25,000 30,000 35,000 40,000 45,000 50,000 55,000 1-Jun8-Jun15-Jun22-Jun29-Jun6-Jul13-Jul20-Jul27-Jul3-Aug10-Aug17-Aug24-Aug31-AugGold Creek Discharge (cfs)Chinook Salmon Passage at Devils Canyon Impediment 2 2012 2013 2014 10,000 15,000 20,000 25,000 30,000 35,000 40,000 45,000 50,000 55,000 1-Jun8-Jun15-Jun22-Jun29-Jun6-Jul13-Jul20-Jul27-Jul3-Aug10-Aug17-Aug24-Aug31-AugGold Creek Discharge (cfs)Chinook Salmon Passage at Devils Canyon Impediment 3 2012 2013 2014 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 128 October 2015 Figure 5.7-1. Destinations for radio-tagged Chinook Salmon released in the Lower River in 2013. Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination. Data from Table 5.2-1 in the ISR. Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 129 October 2015 Figure 5.7-2. Destinations for radio-tagged Chinook Salmon released in the Lower River in 2014. Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination. Data from Table 5.2-1. Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 130 October 2015 Figure 5.7-3. Destinations for radio-tagged Coho Salmon released in the Lower River in 2013 (yellow circles). Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination. Data from Table 5.2-1 in the ISR. Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Page 131 October 2015 Figure 5.7-4. Destinations for radio-tagged Coho Salmon released in the Lower River in 2014 (yellow circles). Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination. Data from Table 5.2-1. Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 October 2015 Appendix A: Fish Capture and Tagging STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 1 October 2015 Table A-1. Number of salmon caught and radio-tagged at two fishwheel sites and from gillnets in the Lower River, PRM 33.4–34.2, 2014. Table A-2. Number of Chinook Salmon caught and radio-tagged at fishwheel sites and in gillnets in the Yentna River (RM 6 and RM 18), 2014. Radio Tag Species Target West Bank East Bank Gillnet Total Chinook Salmon Caught 921 959 168 2,048 Tagged2 700 259 271 129 659 Chum Salmon Caught 2,295 4,282 0 6,577 Coho Salmon Caught 910 603 0 1,513 Tagged 600 337 303 640 Pink Salmon Caught 10,063 3,871 0 13,934 Tagged 200 106 92 198 Sockeye Salmon Caught 396 453 4 853 Total Caught 14,585 10,168 172 24,925 (all species)Tagged 1,500 702 666 129 1,497 1 Total caught includes all adult salmon regardless of size, as well as all recaptured fish. 2 Adult fish measuring 50 cm METF or greater. FishwheelCaught1/ Tagged South North Gillnet Total Yentna River (RM 6)Caught 1,213 1,413 399 3,025 Tagged2 300 95 95 106 296 East West Yentna River (RM 18)Caught 1,440 743 122 2,305 1 Total caught includes all adult salmon regardless of size, as well as all recaptured fish. 2 All tagged Chinook Salmon measured 50 cm METF or greater. Radio Tag Target FishwheelCaught1/ TaggedLocation STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 2 October 2015 Table A-3. Number of salmon radio-tagged at three fishwheel sites and in gillnets in the Middle River, by size category, 2014. Species Large Small Total Large Small Total Large Small Total Large Small Total Large Small Total Chinook Salmon 247 17 264 75 2 77 268 13 281 0 0 0 590 32 622 Chum Salmon 60 0 60 53 0 53 87 0 87 0 0 0 200 0 200 Coho Salmon 73 0 73 14 0 14 125 0 125 18 0 18 230 0 230 Pink Salmon 73 0 73 27 0 27 101 0 101 0 0 0 201 0 201 Sockeye Salmon 54 0 54 89 0 89 57 0 57 0 0 0 200 0 200 Total 507 17 524 258 2 260 638 13 651 18 0 18 1,421 32 1,453 Notes: Large Chinook Salmon measured 50 cm METF or greater; and large Chum, Coho, Pink, and Sockeye salmon measured 40 cm METF or greater. Fishwheels Gill Net All Gear CombinedSite 1 (PRM 124.1)Site 2 (PRM 123.0)Site 3 (PRM 126.0) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A -- Page 3 October 2015 Table A-4. Number of salmon caught in the Lower River and Yentna River and their length statistics, 2014. Location Total Species Name Catch Min Max Mean n Lower River Chinook Salmon (≥ 50 cm)a 1,471 50.0 105.0 67.6 1,431 Chinook Salmon (< 50 cm)a 577 27.0 49.5 39.1 561 Total Chinook Salmon 2,048 Chum Salmon 6,577 ---- Coho Salmon 1,513 28.0 68.0 52.5 1,361 Pink Salmon 13,934 40.0 56.5 44.8 198 Sockeye Salmon 849 32.4 69.0 49.3 592 Yentna River (RM 6) Chinook Salmon (≥ 50 cm)a 1,357 50.0 109.0 66.9 1,355 Chinook Salmon (< 50 cm)a 1,668 21.5 49.5 34.9 1,666 Total Chinook Salmon 3,025 Yentna River (RM 18) Chinook Salmon (≥ 50 cm)a 1,375 50.0 110.0 66.8 1,367 Chinook Salmon (< 50 cm)a 930 24.0 49.5 37.0 924 Total Chinook Salmon 2,305 a Total catch by size category estimated from length samples. Length (cm METF) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A -- Page 4 October 2015 Table A-5. Number of salmon captured at three fishwheel sites and in gillnets in the Middle River, by size category, 2014. Species Large Small Total Large Small Total Large Small Total Large Small Total Large Small Total Chinook Salmon 273 84 357 79 18 97 320 102 422 0 1 1 672 205 877 Chum Salmon 581 0 581 417 0 417 471 0 471 83 0 83 1,552 0 1,552 Coho Salmon 111 1 112 25 0 25 197 1 198 42 0 42 375 2 377 Pink Salmon 3,011 0 3,011 73 0 73 4,389 0 4,389 0 0 0 7,473 0 7,473 Sockeye Salmon 59 4 63 92 2 94 62 4 66 10 1 11 223 11 234 Total 4,035 89 4,124 686 20 706 5,439 107 5,546 135 2 137 10,295 218 10,513 Notes: Totals include all tagged fish recaptured at the fishwheels (30 large Chinook, 2 small Chinook, 8 Chum, 5 Coho, 10 Pink, and 3 Sockeye salmon). Large Chinook Salmon measured 50 cm METF or greater; and large Chum, Coho, Pink, and Sockeye salmon measured 40 cm METF or greater. Gill Net Fishwheels All Gear CombinedSite 1 Site 2 Site 3 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 5 October 2015 Table A-6. Number of fish caught, tagged, and biosampled at the Middle River fishwheels, 2014. Total Radio- Species Name Catch a tagged Min Max Mean n Chinook Salmon (≥ 50 cm)672 590 50 108 71.6 624 589 Chinook Salmon (< 50 cm)204 32 27 49 35.9 198 30 Chum Salmon 1,469 200 45 69 57.9 1,102 194 Coho Salmon 333 212 33 65 54.0 324 199 Pink Salmon 7,473 201 32 67 45.7 955 201 Sockeye Salmon 213 200 33 61 49.3 217 198 Arctic Grayling 17 -13 36 20.9 16 - Burbot 2 -15 15 15.0 2 - Dolly Varden 8 -20 43 32.4 5 6 Longnose Sucker 13 -14 38 29.8 13 - Rainbow Trout 68 -16 53 27.3 63 - Round Whitefish 67 -13 37 26.2 65 - Humpback Whitefish 13 -18 41 31.0 11 9 b Total catch includes recaptures for all salmon, and small (MEF < 40 cm) Coho and Sockeye salmon. a Salmon are measured from mid-eye to fork of tail (MEF); other species are fork lengths (FL). d An additonal 18 Coho Salmon were captured using gillnets and radio-tagged; of which 15 were tissue-sampled. MEF / Fork Length (cm) b Tissue Samplesc c No tissue samples were collected from Arctic Grayling, Burbot, Longnose Sucker, Rainbow Trout, or Round Whitefish because the required sample sizes had been met prior to the 2014 field season. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 6 October 2015 Table A-7. Daily fishing effort and the number of salmon caught and radio-tagged during gillnet operations in the vicinity of Curry, 2014. Date Chinook Salmon (Small) Sockeye Salmon Chum Salmon Coho Salmon 24-Jun 1 1.8 1 0 0 0 0 10-Sep 2 16.4 0 2 16 14 3 12-Sep 2 16.0 0 1 5 5 3 14-Sep 1 8.0 0 0 7 7 3 16-Sep 1 8.0 0 3 1 2 1 18-Sep 2 16.0 0 2 17 9 5 20-Sep 2 16.0 0 2 19 4 2 22-Sep 2 16.0 0 0 9 0 0 24-Sep 2 16.0 0 1 3 0 0 26-Sep 2 16.0 0 0 3 0 0 28-Sep 2 16.0 0 0 3 0 0 30-Sep 1 6.0 0 0 0 1 1 Total 152.1 1 11 83 42 18 Notes: Two of the coho salmon captured on September 20 were recaptures. One of the 11 sockeye salmon caught was a jack (MEF < 40 cm). Fishing sites were located between PRM 121.9 and 126.0. Coho Salmon Tagged Total Effort (net hours) Number of Crews Adult Salmon Catch STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 7 October 2015 Table A-8. Summary of run-timing and catch information for salmon captured in fishwheels located in the Middle River near Curry, by year and species. Species 1981 1982 1983 1984 1985 2012 2013 2014 Date First Fish Caught 6-15 6-15 6-10 6-9 6-20 6-18 6-16 6-11 Date Last Fish Caught 8-20 8-6 7-31 7-29 8-16 8-9 8-28 8-24 Midpoint of Catch Date (50%)6-24 7-3 6-25 6-25 7-9 7-3 6-30 7-2 Peak Daily Catch (Date)6-23 7-4 6-22 6-22 7-8 7-2 7-2 7-1 Peak Daily Catch (Fish)31 55 82 165 98 62 78 70 Total Catch (Fish)284 791 1,064 1,589 1,098 566 952 876 Date First Fish Caught 7-20 7-25 7-10 7-15 7-17 7-10 7-13 7-5 Date Last Fish Caught 9-15 9-14 9-9 9-7 9-12 9-1 9-9 9-5 Midpoint of Catch Date (50%)8-17 8-12 8-3 8-6 8-7 8-7 8-5 8-12 Peak Daily Catch (Date)8-6 8-13 8-2 8-5 8-6 8-2 8-3 8-11 Peak Daily Catch (Fish)87 168 78 366 166 181 259 89 Total Catch (Fish)1,276 1,736 861 4,228 1,305 1,734 3,417 1,469 Date First Fish Caught 8-4 8-2 7-22 7-18 8-3 7-28 7-23 7-22 Date Last Fish Caught 9-19 9-11 9-6 8-31 9-12 8-31 9-21 9-3 Midpoint of Catch Date (50%)8-23 8-18 8-12 8-12 8-18 8-15 8-15 8-20 Peak Daily Catch (Date)8-29 8-19 8-15 8-4 8-20 8/11,8/15 8-17 8-22 Peak Daily Catch (Fish)16 15 10 21 18 21 139 30 Total Catch (Fish)182 229 93 350 203 265 1,723 335 Date First Fish Caught 7-18 7-22 7-20 7-7 7-15 7-16 7-8 7-16 Date Last Fish Caught 8-29 8-26 8-23 8-29 8-28 8-31 9-4 8-30 Midpoint of Catch Date (50%)8-8 8-6 8-1 8-4 8-5 8-6 8-3 7-31 Peak Daily Catch (Date)8-6 8-5 8-1 8-5 8-6 8-2 8-3 7-29 Peak Daily Catch (Fish)39 1,199 67 2,052 147 548 1,422 979 Total Catch (Fish)234 7,302 589 17,394 1,172 4,705 15,695 7,473 Date First Fish Caught 7-17 7-16 7-6 6-26 7-16 7-2 6-15 6-29 Date Last Fish Caught 9-12 9-18 9-4 9-8 9-4 8-22 9-10 9-3 Midpoint of Catch Date (50%)8-5 8-5 8-5 8-1 8-7 7-30 8-5 8-1 Peak Daily Catch (Date)8-5 8-5 8/2,8/12,8/13 7-30 8-6 7-21 8-15 7-28 Peak Daily Catch (Fish)44 16 10 34 29 8 16 13 Total Catch (Fish)469 161 201 379 324 100 276 223 Sockeye Salmon Year Chinook Salmon Chum Salmon Coho Salmon Pink Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 8 October 2015 Table A-9. Comparisons between the cumulative length-frequency distributions of fish sampled in the Lower River and Yentna River using the Kolmogorov-Smirnov (KS) two-sample test, 2014. Sample 1 Sample 2 n1 n 2 Dmax P-value Lower River Chinook Salmon East bank fishwheel West bank fishwheel 937 937 0.111 < 0.001 East bank fishwheel Gillnet 937 164 0.387 < 0.001 West bank fishwheel Gillnet 937 164 0.442 < 0.001 Total catch (METF ≥ 50 cm)Tagged (METF ≥ 50 cm)1,503 656 0.145 < 0.001 Total catch (METF ≥ 58 cm)Tagged (METF ≥ 58 cm)1,086 569 0.030 0.797 Coho Salmon East bank fishwheel West bank fishwheel 551 810 0.083 0.011 Total catch (METF ≥ 40 cm)Tagged (METF ≥ 40 cm)1,349 640 0.023 0.886 Pink Salmon (METF ≥ 40 cm) East bank fishwheel West bank fishwheel 90 107 0.145 0.140 Yentna River Chinook Salmon @ RM6 South bank fishwheel North bank fishwheel 1,213 1,410 0.155 < 0.001 South bank fishwheel Gillnet 1,213 398 0.519 < 0.001 North bank fishwheel Gillnet 1,410 398 0.658 < 0.001 Total catch (METF ≥ 50 cm)Tagged (METF ≥ 50 cm)1,355 1,277 0.005 1.000 Chinook Salmon @ RM18 South bank fishwheel North bank fishwheel 739 1,435 0.250 < 0.001 South bank fishwheel Gillnet 739 118 0.528 < 0.001 North bank fishwheel Gillnet 1,435 118 0.671 < 0.001 Length-Frequency Distributions Sample Size STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 9 October 2015 Table A-10. Comparisons between the cumulative length-frequency distributions of fish sampled in the Middle River using the Kolmogorov-Smirnov (KS) two-sample test, 2014. Sample 1 Sample 2 n 1 n 2 Dmax P-value Chinook Salmon (Large) Site 1 fishwheel catch Site 2 fishwheel catch 339 95 0.12 0.22 Site 1 fishwheel catch Site 3 fishwheel catch 339 388 0.04 1.00 Site 2 fishwheel catch Site 3 fishwheel catch 95 388 0.12 0.19 Total catch (METF ≥ 50 cm)Tagged (METF ≥ 50 cm)624 589 0.01 1.00 Chinook Salmon (Small) Total catch (METF < 50 cm)Tagged (METF < 50 cm)198 32 0.77 < 0.01 Chum Salmon Site 1 fishwheel catch Site 2 fishwheel catch 399 342 0.14 0.00 Site 1 fishwheel catch Site 3 fishwheel catch 399 361 0.04 0.93 Site 2 fishwheel catch Site 3 fishwheel catch 342 361 0.15 < 0.01 Total catch (METF ≥ 40 cm)Tagged (METF ≥ 40 cm)1,102 200 0.08 0.25 Coho Salmon Site 1 fishwheel catch Site 2 fishwheel catch 108 25 0.24 0.20 Site 1 fishwheel catch Site 3 fishwheel catch 108 191 0.05 1.00 Site 2 fishwheel catch Site 3 fishwheel catch 25 191 0.24 0.15 Total catch (METF ≥ 40 cm)Tagged (METF ≥ 40 cm)324 212 0.04 1.00 Pink Salmon Site 1 fishwheel catch Site 2 fishwheel catch 440 66 0.06 1.00 Site 1 fishwheel catch Site 3 fishwheel catch 440 449 0.09 0.04 Site 2 fishwheel catch Site 3 fishwheel catch 66 449 0.1 0.66 Total catch Tagged 955 201 0.15 < 0.01 Sockeye Salmon Site 1 fishwheel catch Site 2 fishwheel catch 60 93 0.1 0.93 Site 1 fishwheel catch Site 3 fishwheel catch 60 64 0.08 1.00 Site 2 fishwheel catch Site 3 fishwheel catch 93 64 0.09 1.00 Total catch (METF ≥ 40 cm)Tagged (METF ≥ 40 cm)217 200 0.04 1.00 Length-Frequency Distributions Sample Size STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 10 October 2015 Figure A-1. Daily fishing effort (hours) at two fishwheel sites in the Lower River, 2014. Figure A-2. Daily gillnet effort (hours) in the Lower River, by mesh size, 2014. Fishing Effort (h) Date (m/d) 0 3 6 9 12 15 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/25Effort West Bank 0 3 6 9 12 15 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/25East Bank 0 1 2 3 4 5 5/225/245/265/285/306/16/36/56/76/96/116/136/156/176/196/216/236/256/27Fishing Effort (h)Date (m/d) 7.5-in Mesh 5.5-in Mesh Gillnet STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 11 October 2015 Figure A-3. Daily fishing effort (hours) at four fishwheel sites in the Yentna River, 2014. Fishing Effort (h) Date (m/d) 0 5 10 15 20 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/25RM 6 -South Bank 0 5 10 15 20 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/25RM 6 -North Bank 0 5 10 15 20 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/25RM 18 -South Bank 0 5 10 15 20 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/25RM 18 -North Bank STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 12 October 2015 Figure A-4. Daily gillnet effort (hours) during tagging (top panel) and recovery (bottom panel) operations in the Yentna River, by mesh size, 2014. Figure A-5. Daily fishing effort (hours) and rotational speed (RPM) at three fishwheel sites in the Middle River, 2014. Fishing Effort (hours) Date (m/d) 0 5 10 15 20 5/225/245/265/285/306/16/36/56/76/96/116/136/156/176/196/216/236/256/276/297.5-in Mesh 5.5-in Mesh Yentna River (RM 6) 0 5 10 15 20 5/225/245/265/285/306/16/36/56/76/96/116/136/156/176/196/216/236/256/276/29Yentna River (RM 18 ) Date (m/d)Fishing Effort (h)Rotational Speed (RPM)0 2 4 6 8 0 4 8 12 16 20 24 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/4Effort RPM Site 1 (west bank) 0 2 4 6 8 0 4 8 12 16 20 24 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/4Site 2 (east bank) 0 2 4 6 8 0 4 8 12 16 20 24 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/4Site 3 (west bank) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 13 October 2015 Figure A-6. Daily number of radio tags applied to adult salmon species captured at two fishwheel sites and in gillnets in the Lower River, 2014. Date (m/d)Radio Tags Applied0 10 20 30 40 5/225/296/56/126/196/267/37/107/177/247/318/78/148/21Gillnet East Bank West Bank Chinook Salmon 0 5 10 15 20 5/225/296/56/126/196/267/37/107/177/247/318/78/148/21Pink Salmon 0 20 40 60 80 5/225/296/56/126/196/267/37/107/177/247/318/78/148/21Coho Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 14 October 2015 Figure A-7. Daily number of radio tags applied to adult salmon species captured at two fishwheel sites and in gillnets in the Yentna River (RM 6), 2014. Radio Tags Applied Date (m/d) 0 5 10 15 20 25 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/15Gillnet North Bank South Bank Chinook Salmon 0 1 2 3 4 5 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/15Coho Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 15 October 2015 Figure A-8. Daily number of radio tags applied to adult salmon species captured at three fishwheel sites and in gillnets in the Middle River, 2014. Date (m/d)Radio Tags Applied0 15 30 45 60 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Gillnet Site 3 (west bank) Site 2 (east bank) Site 1 (west bank) Large Chinook Salmon (≥ 50 cm METF) 0 1 2 3 4 5 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Small Chinook Salmon (< 50 cm METF) 0 5 10 15 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Sockeye Salmon 0 5 10 15 20 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Pink Salmon 0 5 10 15 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Chum Salmon 0 5 10 15 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Coho Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 16 October 2015 Figure A-9. Daily catch-per-unit-effort of adult salmon species at the Lower River fishwheels, and the Susitna River discharge at Sunshine, 2014. Catch-per-unit-effort (fish per hour)Discharge (cfs)Date (m/d) 0 20,000 40,000 60,000 80,000 100,000 0 1 2 3 4 5 6 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/20Chinook Salmon 0 20,000 40,000 60,000 80,000 100,000 0 20 40 60 80 100 120 140 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/20Discharge East West Pink Salmon 0 20,000 40,000 60,000 80,000 100,000 0 20 40 60 80 100 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/20Coho Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 17 October 2015 Figure A-10. Daily catch-per-unit-effort for Chinook and Coho salmon at the Yentna River fishwheels, by site, 2014. Date (m/d)Catch-per-unit-effort (fish per hour)0 2 4 6 8 10 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/25North Fishwheel South Fishwheel RM 6 -Chinook Salmon 0 3 6 9 12 15 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/25RM 6 -Coho Salmon 0 2 4 6 8 10 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/25RM 18 -Chinook Salmon 0 5 10 15 20 25 30 35 5/225/276/16/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/25RM 18 -Coho Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 18 October 2015 Figure A-11. Number of radio tags deployed in species of salmon at the Middle River fishwheels in 2014 relative to fishwheel catches in 2012, 2013, and 2014. Date (m/d)Fishwheel CatchTags Deployed0 5 10 15 20 0 250 500 750 1,000 1,250 1,500 6-66-136-206-277-47-117-187-258-18-88-158-228-299-52012 catch (4705) 2013 catch (15695) 2014 catch (7472) 2014 tags (200) Pink Salmon 0 2 4 6 8 10 12 14 0 50 100 150 200 250 300 6-66-136-206-277-47-117-187-258-18-88-158-228-299-52012 catch (1734) 2013 catch (3417) 2014 catch (1469) 2014 tags (200) Chum Salmon 0 3 6 9 12 15 0 50 100 150 6-66-136-206-277-47-117-187-258-18-88-158-228-299-52012 catch (264) 2013 catch (1711) 2014 catch (334) 2014 tags (212) Coho Salmon 0 10 20 30 40 50 60 0 10 20 30 40 50 60 70 6-66-136-206-277-47-117-187-258-18-88-158-228-299-52012 catch (422) 2013 catch (616) 2014 catch (672) 2014 tags (590) Chinook Salmon 0 2 4 6 8 10 12 14 0 2 4 6 8 10 12 14 6-66-136-206-277-47-117-187-258-18-88-158-228-299-52012 catch (92) 2013 catch (176) 2014 catch (213) 2014 tags (200) Sockeye Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 19 October 2015 Figure A-12. Daily catch-per-unit-effort at the Middle River fishwheels, by species, and the Susitna River discharge at Gold Creek, 2014. Date (m/d)Catch-per-unit-effort (fish per hour)Discharge (cfs)0 10,000 20,000 30,000 40,000 50,000 0.0 0.5 1.0 1.5 2.0 6/96/146/196/246/297/47/97/147/197/247/298/38/88/138/188/238/289/29/7Discharge Site 1 Site 2 Site 3 Large Chinook Salmon 0 10,000 20,000 30,000 40,000 50,000 0.0 0.2 0.4 0.6 0.8 6/96/146/196/246/297/47/97/147/197/247/298/38/88/138/188/238/289/29/7Sockeye Salmon 0 10,000 20,000 30,000 40,000 50,000 0 10 20 30 40 50 60 6/96/146/196/246/297/47/97/147/197/247/298/38/88/138/188/238/289/29/7Pink Salmon 0 10,000 20,000 30,000 40,000 50,000 0 1 2 3 4 6/96/146/196/246/297/47/97/147/197/247/298/38/88/138/188/238/289/29/7Chum Salmon 0 10,000 20,000 30,000 40,000 50,000 0.0 0.5 1.0 1.5 2.0 6/96/146/196/246/297/47/97/147/197/247/298/38/88/138/188/238/289/29/7Coho Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 20 October 2015 Figure A-13. Comparison of Chinook Salmon catches (top panel), relative proportion of catches (middle panel), and cumulative proportion of catches (bottom panel), at the Middle River fishwheels near Curry, by year. These data include Chinook Salmon of all size categories and catches at two (1981-2012) or three (2013 and 2014) fishwheels. Date (m/d) 0.0 0.2 0.4 0.6 0.8 1.0 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Cumulative Proportion of Catch0 30 60 90 120 150 180 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Catch (# fish)1981 1982 1983 1984 1985 2012 2013 2014 Chinook Salmon 0.00 0.02 0.04 0.06 0.08 0.10 0.12 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Relative Proportion of Catch STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 21 October 2015 Figure A-14. Comparison of Chum Salmon catches (top panel), relative proportion of catches (middle panel), and cumulative proportion of catches (bottom panel), at the Middle River fishwheels near Curry, by year. These data include adult Chum Salmon of all size categories and catches at two (1981-2012) or three (2013 and 2014) fishwheels. Date (m/d) 0.0 0.2 0.4 0.6 0.8 1.0 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Cumulative Proportion of Catch0 50 100 150 200 250 300 350 400 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Catch (# fish)1981 1982 1983 1984 1985 2012 2013 2014 Chum Salmon 0.00 0.02 0.04 0.06 0.08 0.10 0.12 0.14 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Relative Proportion of Catch STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 22 October 2015 Figure A-15. Comparison of Coho Salmon catches (top panel), relative proportion of catches (middle panel), and cumulative proportion of catches (bottom panel), at the Middle River fishwheels near Curry, by year. These data include adult Coho Salmon of all size categories and catches at two (1981-2012) or three (2013 and 2014) fishwheels. Date (m/d) 0.0 0.2 0.4 0.6 0.8 1.0 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Cumulative Proportion of Catch0 20 40 60 80 100 120 140 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Catch (# fish)1981 1982 1983 1984 1985 2012 2013 2014 Coho Salmon 0.00 0.02 0.04 0.06 0.08 0.10 0.12 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Relative Proportion of Catch STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 23 October 2015 Figure A-16. Comparison of Pink Salmon catches (top panel), relative proportion of catches (middle panel), and cumulative proportion of catches (bottom panel), at the Middle River fishwheels near Curry, by year. These data include adult Pink Salmon of all size categories and catches at two (1981-2012) or three (2013 and 2014) fishwheels. Date (m/d) 0.0 0.2 0.4 0.6 0.8 1.0 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Cumulative Proportion of Catch0 500 1,000 1,500 2,000 2,500 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Catch (# fish)1981 1982 1983 1984 1985 2012 2013 2014 Pink Salmon 0.00 0.02 0.04 0.06 0.08 0.10 0.12 0.14 0.16 0.18 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Relative Proportion of Catch STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 24 October 2015 Figure A-17. Comparison of Sockeye Salmon catches (top panel), relative proportion of catches (middle panel), and cumulative proportion of catches (bottom panel), at the Middle River fishwheels near Curry, by year. These data include adult Sockeye Salmon of all size categories and catches at two (1981-2012) or three (2013 and 2014) fishwheels. Date (m/d) 0.0 0.2 0.4 0.6 0.8 1.0 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Cumulative Proportion of Catch0 10 20 30 40 50 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Catch (# fish)1981 1982 1983 1984 1985 2012 2013 2014 Sockeye Salmon 0.00 0.02 0.04 0.06 0.08 0.10 6/66/116/166/216/267/17/67/117/167/217/267/318/58/108/158/208/258/309/49/99/149/199/249/29Relative Proportion of Catch STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 25 October 2015 Figure A-18. Daily sampling effort, and the amount of imagery reviewed (review effort), for an ARIS sonar unit operated immediately downstream of the fishwheel at Site 1 in the Middle River, 2014. Figure A-19. Catch-per-unit-effort, or the number of targets counted per hour of imagery reviewed, on the ARIS unit located immediately downstream of the Site 1 fishwheel, 2014. ARIS data were included, regardless of whether the Site 1 fishwheel was operational. Imagery collected after June 17 was subsampled and the counts were expanded to full hourly counts. From June 3 to June 25, targets measuring 50 cm or greater were included; whereas from August 29 to September 30, targets measuring 40 cm or greater were included. Data on two days when CPUE was less than zero were excluded (-0.8 fish/hour on September 25 and -1.1 fish/hour on September 27). Turbidity measurements recorded at Site 1 and an overlay of Susitna River discharge at the Gold Creek gauge are also shown. 0 4 8 12 16 20 24 6/36/86/136/186/236/287/37/87/137/187/237/288/28/78/128/178/228/279/19/69/119/169/219/26Effort (h)Date (m/d) Sampling Effort (h) Review Effort (h) Discharge at Gold Cr 0 50 100 150 200 250 300 350 0 5 10 15 20 25 30 35 40 45 6/36/86/136/186/236/287/37/87/137/187/237/288/28/78/128/178/228/279/19/69/119/169/219/26Turbidity (NTU)ARIS CPUE (fish/hour)Date (m/d) CPUE Turbidity STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 26 October 2015 Figure A-20. Comparison of the catch-per-unit-effort of adult salmon at the Site 1 fishwheel and concurrent net upstream counts of fish on the ARIS unit located immediately downstream of the fishwheel, 2014. Imagery collected after June 17 was subsampled and the counts were expanded to full hourly counts. From June 3 to June 25, targets measuring 50 cm or greater were included; whereas from August 29 to September 30, targets measuring 40 cm or greater were included. An overlay of Susitna River discharge at the Gold Creek gauge is also shown. Figure A-21. Relative percentage of fish counted using ARIS at Site 1 as a function of the distance where they were first detected in the field of view, by time period, 2014. From June 3 to June 25, targets measuring 50 cm or greater were included; whereas from August 29 to September 30, targets measuring 40 cm or greater were included. Discharges (cfs) refer to the Susitna River at the Gold Creek gauge. Discharge at Gold Cr 0.0 0.2 0.4 0.6 0.8 1.0 1.2 0 50 100 150 200 250 6/36/86/136/186/236/287/37/87/137/187/237/288/28/78/128/178/228/279/19/69/119/169/219/26Fishwheel CPUE (fish/hour)Net Upstream ARIS CountDate (m/d) ARIS Count Fishwheel CPUE 0 3 6 9 12 15 18 21 24 27 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16Percent of ARIS CountsDistance from ARIS Unit (m) June 3-15, n = 255 (13,300-19,800 cfs) June 16-25, n = 770 (21,800-25,700 cfs) Aug 29-Sep 30, n = 1305 (6,190-22,500 cfs) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 27 October 2015 Figure A-22. Diel migration of upstream-moving fish counted using ARIS at Site 1, by size category and time period, 2014. Top panel: 24 hours of imagery collected, all imagery reviewed. Middle panel: 24 hours of imagery collected, 8 hours of imagery reviewed each day. Chinook Salmon were the main species being captured at the Site 1 fishwheel through late June. Bottom panel: 24 hours of imagery collected, 8 hours of imagery reviewed each day. Chum and Coho salmon were presumably the predominant species migrating through the Middle River in September. ARIS CountsTime of Day (hour) 0 10 20 30 40 01234567891011121314151617181920212223METF ≥ 50 cm (n = 522) METF 40-49 cm (n = 29) June 4-9 and June 12-17 0 5 10 15 20 25 30 01234567891011121314151617181920212223METF ≥ 50 cm (n = 342) METF = 40-49 cm (n = 37) June 19-24 0 10 20 30 40 50 60 01234567891011121314151617181920212223METF ≥ 50 cm (n = 864) METF = 40-49 cm (n = 119) September 1 -20 and September 22 -29 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 28 October 2015 Figure A-23. Cumulative length-frequency distributions for Chinook Salmon captured in the Lower River, by capture site, 2014. Figure A-24. Cumulative length-frequency distributions for Chinook and Pink salmon caught and radio- tagged in the Lower River, by species, 2014. Mid-eye to Fork Length (cm)Cumulative Proportion0.0 0.2 0.4 0.6 0.8 1.0 20 30 40 50 60 70 80 90 100 110 Chinook Salmon West Bank East Bank Gillnet Mid-eye to Fork Length (cm)Cumulative Proportion0.0 0.2 0.4 0.6 0.8 1.0 30 40 50 60 70 80 90 100 110 120 130 Chinook Salmon Caught Radio-tagged 0.0 0.2 0.4 0.6 0.8 1.0 30 35 40 45 50 55 60 Pink Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 29 October 2015 Figure A-25. Cumulative length-frequency distributions for Chinook Salmon radio-tagged in the Lower River and inspected and recaptured at the Deshka River and Montana Creek weir sites, 2014. Figure A-26. Cumulative length-frequency distributions for Chinook Salmon captured at RM 6 (left panel) and RM 18 (right panel) in the Yentna River, 2014. Cumulative Proportion Mid-eye to Fork Length (cm) 0.0 0.2 0.4 0.6 0.8 1.0 30 40 50 60 70 80 90 100 110 120 Montana Weir Chinook Salmon Tagged in Lower River Inspected at Weir Recaptures at Weir 0.0 0.2 0.4 0.6 0.8 1.0 30 40 50 60 70 80 90 100 110 120 Deshka Weir Chinook Salmon Cumulative ProportionMid-eye to Fork Length (cm) 0.0 0.2 0.4 0.6 0.8 1.0 20 30 40 50 60 70 80 90 100 110 120 RM 6 South Bank North Bank Gillnet 0.0 0.2 0.4 0.6 0.8 1.0 20 30 40 50 60 70 80 90 100 110 120 RM 18 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 30 October 2015 Figure A-27. Cumulative length-frequency distributions for Chinook Salmon measuring 50 cm METF or greater that were caught and dart-tagged in the Yentna River (RM 6), 2014. Figure A-28. Cumulative length-frequency distributions for Chinook Salmon dart-tagged at Yentna RM 6 and inspected and recaptured at Yentna RM 18 (fishwheels and gillnets combined), 2014. 0.0 0.2 0.4 0.6 0.8 1.0 50 60 70 80 90 100 110Cumulative ProportionMid-eye to Fork Length (cm) Caught Dart-tagged Mid-eye to Fork Length (cm)Cumulative Proportion0.0 0.2 0.4 0.6 0.8 1.0 20 30 40 50 60 70 80 90 100 110 120 Chinook Salmon Tagged at Yentna RM 6 Inspected for Tags Recaptures STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 31 October 2015 Figure A-29. Cumulative length-frequency distributions for salmon captured in the Middle River fishwheels, by species and capture site, 2014. Mid-eye to Fork Length (cm)Cumulative Proportion0.0 0.2 0.4 0.6 0.8 1.0 25 35 45 55 65 75 85 95 105 115 Chinook Salmon (Large) Site 1 Site 2 Site 3 0.0 0.2 0.4 0.6 0.8 1.0 30 35 40 45 50 55 60 65 70 Sockeye Salmon 0.0 0.2 0.4 0.6 0.8 1.0 30 35 40 45 50 55 60 65 70 Pink Salmon 0.0 0.2 0.4 0.6 0.8 1.0 30 35 40 45 50 55 60 65 70 Coho Salmon 0.0 0.2 0.4 0.6 0.8 1.0 30 35 40 45 50 55 60 65 70 Chum Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix A – Page 32 October 2015 Figure A-30. Cumulative length-frequency distributions for salmon caught and radio-tagged in the Middle River, by species, 2014. Mid-eye to Fork Length (cm)Cumulative Proportion0.0 0.2 0.4 0.6 0.8 1.0 45 55 65 75 85 95 105 115 Chinook Salmon (≥ 50 cm METF) Captured in the Middle River Radio-tagged 0.0 0.2 0.4 0.6 0.8 1.0 30 35 40 45 50 55 60 65 70 75 Sockeye Salmon 0.0 0.2 0.4 0.6 0.8 1.0 30 35 40 45 50 55 60 Pink Salmon 0.0 0.2 0.4 0.6 0.8 1.0 30 35 40 45 50 55 60 65 70 75 80 Chum Salmon 0.0 0.2 0.4 0.6 0.8 1.0 30 35 40 45 50 55 60 65 70 Coho Salmon 0.0 0.2 0.4 0.6 0.8 1.0 25 30 35 40 45 50 Chinook Salmon (< 50 cm METF) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 October 2015 Appendix B: Daily fish passage at weir and sonar sites in the Lower and Middle rivers STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix B – Page 1 October 2015 Table B-1. Deshka River weir daily passage rates and tag recaptures, by species, 2014. Daily Cum.Radio Daily Cum.Daily Cum.Daily Cum.Daily Cum. Date Count Count Tags Count Count Count Count Count Count Count Count 19-May 2 2 0 0 0 0 0 0 0 20-May 3 5 0 0 0 0 0 0 0 0 21-May 4 9 0 0 0 0 0 0 0 0 22-May 10 19 0 0 0 0 0 0 0 0 23-May 8 27 1 0 0 0 0 0 0 0 24-May 9 36 1 0 0 0 0 0 0 0 25-May 15 51 0 0 0 0 0 0 0 0 26-May 25 76 0 0 0 0 0 0 0 0 27-May 6 82 2 0 0 0 0 0 0 0 28-May 7 89 0 0 0 0 0 0 0 0 29-May 93 182 1 0 0 0 0 0 0 0 0 30-May 14 196 4 0 0 0 0 0 0 0 0 31-May 39 235 3 0 0 0 0 0 0 0 0 01-Jun 13 248 4 0 0 0 0 0 0 0 0 02-Jun 166 414 6 0 0 0 0 0 0 0 0 03-Jun 214 628 9 0 0 0 0 0 0 0 0 04-Jun 86 714 8 0 0 0 0 0 0 0 0 05-Jun 454 1,168 8 0 0 0 0 0 0 0 0 06-Jun 735 1,903 10 0 0 0 0 0 0 0 0 07-Jun 715 2,618 5 0 0 0 0 0 0 0 0 08-Jun 608 3,226 6 0 0 0 0 0 0 0 0 09-Jun 1,065 4,291 5 0 0 0 0 0 0 0 0 10-Jun 2,279 6,570 8 0 0 0 0 0 0 0 0 11-Jun 1,463 8,033 7 0 0 0 0 0 0 0 0 12-Jun 1,033 9,066 5 0 0 0 0 0 0 0 0 13-Jun 994 10,060 8 0 0 0 0 0 0 0 0 14-Jun 666 10,726 1 0 0 0 0 0 0 0 0 15-Jun 758 11,484 0 0 0 0 0 0 0 0 0 16-Jun 955 12,439 4 0 0 0 0 0 0 0 0 17-Jun 712 13,151 3 0 0 0 0 0 0 0 0 18-Jun 455 13,606 5 0 0 0 0 0 0 0 0 Chinook Salmon Coho Salmon Chum Salmon Sockeye SalmonPink Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix B – Page 2 October 2015 Table B-1. Continued. Daily Cum.Radio Daily Cum.Daily Cum.Daily Cum.Daily Cum. Date Count Count Tags Count Count Count Count Count Count Count Count 19-Jun 320 13,926 1 0 0 0 0 0 0 0 0 20-Jun 432 14,358 1 0 0 0 0 0 0 0 0 21-Jun 278 14,636 5 0 0 0 0 0 0 0 0 22-Jun 206 14,842 1 0 0 0 0 0 0 0 0 23-Jun 556 15,398 0 0 0 0 0 0 0 0 0 24-Jun 92 15,490 2 0 0 0 0 0 0 0 0 25-Jun 163 15,653 0 0 0 0 0 0 0 0 0 26-Jun 94 15,747 0 0 0 0 0 0 0 0 0 27-Jun 14 15,761 0 0 0 0 0 0 0 0 0 28-Jun 0 15,761 0 0 0 0 0 0 0 0 0 29-Jun 0 15,761 1 0 0 0 0 0 0 0 0 30-Jun 10 15,771 0 0 0 0 0 0 0 0 0 01-Jul 36 15,807 0 0 0 0 0 0 0 0 0 02-Jul 37 15,844 0 0 0 0 0 0 0 0 0 03-Jul 101 15,945 0 0 0 0 0 0 0 0 0 04-Jul 11 15,956 0 5 5 1 1 0 0 0 0 05-Jul 43 15,999 0 2 7 0 1 0 0 0 0 06-Jul 18 16,017 0 0 7 9 10 0 0 0 0 07-Jul 15 16,032 0 2 9 21 31 0 0 0 0 08-Jul 11 16,043 0 4 13 12 43 0 0 0 0 09-Jul 10 16,053 0 0 13 6 49 0 0 0 0 10-Jul 33 16,086 0 3 16 26 75 2 2 0 0 11-Jul 28 16,114 0 11 27 68 143 0 2 0 0 12-Jul 19 16,133 0 16 43 207 350 1 3 1 1 13-Jul 29 16,162 0 18 61 156 506 0 3 0 1 14-Jul 4 16,166 0 9 70 172 678 1 4 0 1 15-Jul 11 16,177 0 23 93 564 1,242 2 6 0 1 16-Jul 11 16,188 0 36 129 3,012 4,254 7 13 6 7 17-Jul 13 16,201 0 11 140 3,100 7,354 13 26 0 7 18-Jul 10 16,211 0 34 174 6,466 13,820 5 31 0 7 19-Jul 4 16,215 0 85 259 10,630 24,450 4 35 0 7 Chinook Salmon Coho Salmon Chum Salmon Sockeye SalmonPink Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix B – Page 3 October 2015 Table B-1. Continued. Daily Cum.Radio Daily Cum.Daily Cum.Daily Cum.Daily Cum. Date Count Count Tags Count Count Count Count Count Count Count Count 20-Jul 2 16,217 0 88 347 4,350 28,800 1 36 0 7 21-Jul 12 16,229 0 132 479 11,144 39,944 5 41 4 11 22-Jul 3 16,232 0 82 561 3,351 43,295 2 43 0 11 23-Jul 7 16,239 0 155 716 10,275 53,570 2 45 0 11 24-Jul 0 16,239 0 139 855 7,837 61,407 4 49 2 13 25-Jul 2 16,241 0 136 991 3,686 65,093 2 51 3 16 26-Jul 3 16,244 0 487 1,478 3,367 68,460 3 54 1 17 27-Jul 3 16,247 0 296 1,774 2,976 71,436 2 56 1 18 28-Jul 1 16,248 0 79 1,853 1,610 73,046 1 57 0 18 29-Jul 0 16,248 0 58 1,911 731 73,777 1 58 1 19 30-Jul 2 16,250 0 412 2,323 1,650 75,427 3 61 0 19 31-Jul 2 16,252 0 123 2,446 642 76,069 4 65 0 19 01-Aug 0 16,252 0 19 2,465 107 76,176 1 66 0 19 02-Aug 0 16,252 0 80 2,545 482 76,658 1 67 0 19 03-Aug 2 16,254 0 65 2,610 240 76,898 0 67 0 19 04-Aug 5 16,259 0 12 2,622 144 77,042 9 76 0 19 05-Aug 2 16,261 0 152 2,774 194 77,236 5 81 2 21 06-Aug 1 16,262 0 308 3,082 192 77,428 0 81 0 21 07-Aug 2 16,264 0 262 3,344 152 77,580 1 82 1 22 08-Aug 1 16,265 0 364 3,708 134 77,714 3 85 0 22 09-Aug 3 16,268 0 385 4,093 79 77,793 1 86 0 22 10-Aug 0 16,268 0 376 4,469 73 77,866 0 86 0 22 11-Aug 2 16,270 0 418 4,887 31 77,897 2 88 2 24 12-Aug 3 16,273 0 177 5,064 15 77,912 0 88 1 25 13-Aug 3 16,276 0 210 5,274 14 77,926 0 88 0 25 14-Aug 6 16,282 0 179 5,453 41 77,967 2 90 0 25 15-Aug 5 16,287 0 265 5,718 19 77,986 3 93 0 25 16-Aug 5 16,292 0 380 6,098 17 78,003 2 95 0 25 17-Aug 7 16,299 0 2,810 8,908 34 78,037 5 100 0 25 18-Aug 7 16,306 0 153 9,061 11 78,048 4 104 0 25 19-Aug 8 16,314 0 184 9,245 19 78,067 2 106 0 25 Chinook Salmon Coho Salmon Chum Salmon Sockeye SalmonPink Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix B – Page 4 October 2015 Table B-1. Continued. Daily Cum.Radio Daily Cum.Daily Cum.Daily Cum.Daily Cum. Date Count Count Tags Count Count Count Count Count Count Count Count 20-Aug 1 16,315 0 128 9,373 12 78,079 0 106 0 25 21-Aug 5 16,320 0 103 9,476 3 78,082 1 107 0 25 22-Aug 1 16,321 0 130 9,606 2 78,084 0 107 0 25 23-Aug 0 16,321 0 137 9,743 3 78,087 0 107 0 25 24-Aug 0 16,321 0 102 9,845 1 78,088 1 108 0 25 25-Aug 2 16,323 0 142 9,987 2 78,090 1 109 1 26 26-Aug 0 16,323 0 159 10,146 1 78,091 0 109 0 26 27-Aug 2 16,325 0 284 10,430 3 78,094 1 110 0 26 28-Aug 5 16,330 0 418 10,848 8 78,102 0 110 0 26 29-Aug 0 16,330 0 649 11,497 9 78,111 0 110 0 26 30-Aug 0 16,330 0 0 11,497 0 78,111 0 110 0 26 31-Aug 2 16,332 0 28 11,525 0 78,111 0 110 0 26 01-Sep 3 16,335 0 31 11,556 0 78,111 0 110 0 26 02-Sep 0 16,335 0 22 11,578 0 78,111 0 110 0 26 Total 16,335 125 11,578 78,111 110 26 Chinook Salmon Coho Salmon Chum Salmon Sockeye SalmonPink Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix B – Page 5 October 2015 Table B-2. Montana Creek weir daily passage rates and tag recaptures, by species, 2014. Daily Cum.Radio Daily Cum.Daily Cum.Daily Cum. Date Count Count Tags Count Count Count Count Count Count Comment 29-May 1 30-May 0 31-May 0 01-Jun 1 02-Jun 0 03-Jun 0 04-Jun 0 0 0 0 0 0 0 0 0 Weir installed 6/4 05-Jun 0 0 1 0 0 0 0 0 0 06-Jun 0 0 0 0 0 0 0 0 0 07-Jun 0 0 1 0 0 0 0 0 0 08-Jun 0 0 1 0 0 0 0 0 0 09-Jun 0 0 2 0 0 0 0 0 0 10-Jun 0 0 2 0 0 0 0 0 0 11-Jun 0 0 0 0 0 0 0 0 0 12-Jun 0 0 1 0 0 0 0 0 0 13-Jun 0 0 0 0 0 0 0 0 0 14-Jun 0 0 0 0 0 0 0 0 0 15-Jun 0 0 2 0 0 0 0 0 0 16-Jun 0 0 0 0 0 0 0 0 0 17-Jun 0 0 2 0 0 0 0 0 0 18-Jun 0 0 0 0 0 0 0 0 0 19-Jun 0 0 0 0 0 0 0 0 0 20-Jun 0 0 0 0 0 0 0 0 0 21-Jun 0 0 0 0 0 0 0 0 0 22-Jun 0 1 0 0 0 0 0 0 0 23-Jun 1 1 0 0 0 0 0 0 0 24-Jun 0 4 0 0 0 0 0 0 0 25-Jun 3 4 0 0 0 0 0 0 0 26-Jun 0 4 1 0 0 0 0 0 0 Weir topped 27-Jun 0 4 0 0 0 0 0 0 0 Weir topped 28-Jun 0 4 0 0 0 0 0 0 0 Weir topped Chinook Salmon Coho Salmon Pink Salmon Chum Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix B – Page 6 October 2015 Table B-2. Continued. Daily Cum.Radio Daily Cum.Daily Cum.Daily Cum. Date Count Count Tags Count Count Count Count Count Count Comment 29-Jun 0 4 0 0 0 0 0 0 0 Weir topped 30-Jun 0 4 0 0 0 0 0 0 0 Weir topped 01-Jul 0 11 0 0 0 0 0 0 0 02-Jul 7 25 0 0 0 0 0 0 0 03-Jul 14 57 0 0 0 0 0 0 0 04-Jul 32 85 0 0 0 0 0 0 0 05-Jul 28 119 0 0 0 0 0 0 0 06-Jul 34 253 0 0 0 0 0 0 0 07-Jul 134 320 0 0 0 0 0 1 1 08-Jul 67 324 0 0 0 0 0 0 1 09-Jul 4 403 0 0 0 0 0 0 1 10-Jul 79 455 0 0 0 0 0 2 3 11-Jul 52 477 0 0 0 0 0 10 13 12-Jul 22 493 0 0 0 0 0 1 14 13-Jul 16 545 0 0 0 0 0 2 16 14-Jul 52 594 0 0 0 0 0 9 25 15-Jul 49 2 0 0 0 0 0 3 28 16-Jul 12 615 0 0 0 0 0 4 32 17-Jul 9 645 0 0 0 0 0 12 44 18-Jul 30 647 0 0 0 0 0 9 53 19-Jul 2 768 0 0 0 0 0 8 61 20-Jul 121 797 0 0 0 0 0 28 89 21-Jul 29 823 0 0 0 1 1 6 95 22-Jul 26 900 0 0 0 0 1 16 111 23-Jul 77 905 0 0 0 2 3 45 156 24-Jul 5 997 0 0 0 8 11 43 199 25-Jul 92 1,031 0 0 0 118 129 161 360 26-Jul 34 1,033 0 0 0 34 163 74 434 27-Jul 2 1,040 0 0 0 17 180 15 449 28-Jul 7 1,071 0 0 0 23 203 59 508 29-Jul 31 1,120 0 0 0 36 239 121 629 Chinook Salmon Coho Salmon Pink Salmon Chum Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix B – Page 7 October 2015 Table B-2. Continued. Daily Cum.Radio Daily Cum.Daily Cum.Daily Cum. Date Count Count Tags Count Count Count Count Count Count Comment 30-Jul 49 1,129 0 0 0 28 267 71 700 31-Jul 9 1,134 0 0 0 34 301 89 789 01-Aug 5 1,139 0 0 0 64 365 159 948 02-Aug 5 1,146 0 0 0 69 434 189 1,137 03-Aug 7 1,159 0 2 2 46 480 202 1,339 04-Aug 13 1,172 0 0 2 68 548 372 1,711 05-Aug 13 1,177 0 0 2 91 639 357 2,068 06-Aug 5 1,188 0 1 3 70 709 152 2,220 07-Aug 11 1,192 0 3 6 59 768 239 2,459 08-Aug 4 1,193 0 0 6 49 817 97 2,556 09-Aug 1 1,193 0 1 7 67 884 171 2,727 10-Aug 0 1,196 0 2 9 37 921 127 2,854 11-Aug 3 1,199 0 3 12 42 963 179 3,033 12-Aug 3 1,200 0 7 19 49 1,012 140 3,173 13-Aug 1 1,201 0 3 22 35 1,047 227 3,400 14-Aug 1 1,203 0 7 29 29 1,076 196 3,596 15-Aug 2 1,204 0 21 50 57 1,133 524 4,120 16-Aug 1 1,205 0 5 55 12 1,145 90 4,210 17-Aug 1 1,207 0 6 61 17 1,162 60 4,270 18-Aug 2 1,208 0 2 63 21 1,183 66 4,336 19-Aug 1 1,208 0 6 69 12 1,195 114 4,450 20-Aug 0 1,208 0 3 72 8 1,203 90 4,540 21-Aug 0 1,208 0 6 78 4 1,207 111 4,651 22-Aug 0 1,208 0 4 82 10 1,217 134 4,785 23-Aug 0 1,208 0 1 83 3 1,220 149 4,934 24-Aug 0 1,211 0 3 86 3 1,223 135 5,069 25-Aug 3 1,213 0 32 118 9 1,232 249 5,318 26-Aug 2 1,215 0 5 123 4 1,236 92 5,410 27-Aug 2 1,215 0 52 175 6 1,242 134 5,544 Weir partially topped 28-Aug 0 1,215 0 87 262 2 1,244 112 5,656 Weir topped 29-Aug 0 1,215 0 1 263 0 1,244 3 5,659 Weir partially topped Chinook Salmon Coho Salmon Pink Salmon Chum Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix B – Page 8 October 2015 Table B-2. Continued. Daily Cum.Radio Daily Cum.Daily Cum.Daily Cum. Date Count Count Tags Count Count Count Count Count Count Comment 30-Aug 0 1,215 0 3 266 1 1,245 11 5,670 Weir topped 31-Aug 0 1,215 0 4 270 0 1,245 40 5,710 Weir topped until 15:30 01-Sep 0 1,215 0 2 272 8 1,253 50 5,760 02-Sep 0 1,215 0 26 298 3 1,256 97 5,857 03-Sep 0 1,215 0 62 360 6 1,262 71 5,928 04-Sep 0 1,215 0 9 369 0 1,262 38 5,966 05-Sep 0 1,215 0 46 415 3 1,265 69 6,035 06-Sep 0 1,215 0 59 474 1 1,266 110 6,145 07-Sep 0 1,215 0 12 486 0 1,266 46 6,191 08-Sep 0 1,215 0 8 494 2 1,268 45 6,236 09-Sep 0 1,215 0 16 510 0 1,268 60 6,296 10-Sep 0 1,217 0 113 623 1 1,269 40 6,336 11-Sep 2 1,217 0 82 705 1 1,270 48 6,384 12-Sep 0 1,217 0 12 717 0 1,270 18 6,402 13-Sep 0 1,217 0 41 758 0 1,270 17 6,419 14-Sep 0 1,217 0 45 803 0 1,270 25 6,444 15-Sep 0 1,217 0 31 834 0 1,270 17 6,461 16-Sep 0 1,217 0 23 857 0 1,270 15 6,476 17-Sep 0 1,217 0 16 873 0 1,270 15 6,491 18-Sep 0 1,217 0 32 905 0 1,270 12 6,503 19-Sep 0 1,217 0 25 930 0 1,270 5 6,508 Weir partially topped 20-Sep 0 1,217 0 0 930 0 1,270 0 6,508 21-Sep 0 1,217 0 4 934 0 1,270 3 6,511 Total 1,217 15 934 1,270 6,511 Chinook Salmon Coho Salmon Pink Salmon Chum Salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix B – Page 9 October 2015 Table B-3. Length statistics for tagged and untagged adult salmon sampled at the Deshka River and Montana Creek weirs, by species, 2014. Table B-4. Daily amount of video imagery collected and reviewed at the Indian River weir, and the net upstream count of fish, by species, 2014. Species Tagged Not Tagged Total Tagged Not Tagged Total Chinook Salmon (≥ 50 cm METF) Min 50.0 50.0 50.0 50.0 51.0 50.0 Max 98.0 94.5 98.0 88.5 107.0 107.0 Mean 66.1 68.8 67.9 66.4 69.1 68.7 n 125 251 376 15 226 241 Chinook Salmon (< 50 cm METF) Min -34.5 34.5 -48.0 48.0 Max -49.5 49.5 -48.0 48.0 Mean -46.8 46.8 --- n -45 45 -1 1 Coho Salmon (≥ 40 cm METF) Min 40.0 44.0 40.0 52.0 40.0 40.0 Max 61.0 64.5 64.5 58.5 64.0 64.0 Mean 54.4 56.2 55.8 55.1 56.6 56.6 n 68 304 372 4 213 217 Deshka River Weir Montana Creek Weir Date Sample Effort (h) Review Effort (h) Rainbow Trout Round Whitefish 22-Jun 10.5 10.5 0 0 23-Jun 24.0 24.0 0 1 24-Jun 24.0 24.0 1 0 25-Jun 24.0 24.0 2 1 26-Jun 6.5 1.3 0 0 Total 89.0 83.8 3 2 Net Upstream Count STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix B – Page 10 October 2015 Table B-5. Daily number of Chinook Salmon inspected for tags, and the number of dart-tag recaptures, at RM 18 sites on the Yentna River, 2014. Daily Cum.Dart Cum. Date Count Count Tags Tags 24-May 3 3 0 0 25-May 9 12 0 0 26-May 16 28 0 0 27-May 38 66 3 3 28-May 17 83 1 4 29-May 10 93 0 4 30-May 16 109 0 4 31-May 4 113 1 5 01-Jun 6 119 0 5 02-Jun 19 138 1 6 03-Jun 118 256 3 9 04-Jun 149 405 1 10 05-Jun 171 576 3 13 06-Jun 135 711 5 18 07-Jun 114 825 0 18 08-Jun 113 938 5 23 09-Jun 112 1,050 4 27 10-Jun 104 1,154 3 30 11-Jun 94 1,248 2 32 12-Jun 127 1,375 3 35 13-Jun 131 1,506 2 37 14-Jun 99 1,605 5 42 15-Jun 110 1,715 5 47 16-Jun 91 1,806 2 49 17-Jun 48 1,854 2 51 18-Jun 40 1,894 3 54 19-Jun 44 1,938 1 55 20-Jun 66 2,004 1 56 21-Jun 53 2,057 1 57 22-Jun 20 2,077 0 57 23-Jun 23 2,100 0 57 24-Jun 43 2,143 1 58 25-Jun 45 2,188 2 60 26-Jun 16 2,204 0 60 27-Jun 5 2,209 0 60 28-Jun 5 2,214 0 60 29-Jun 4 2,218 0 60 30-Jun 10 2,228 1 61 01-Jul 3 2,231 0 61 02-Jul 5 2,236 0 61 03-Jul 2 2,238 0 61 04-Jul 9 2,247 0 61 05-Jul 6 2,253 0 61 06-Jul 5 2,258 0 61 07-Jul 5 2,263 0 61 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix B – Page 11 October 2015 Table B-5. Continued. Daily Cum.Dart Cum. Date Count Count Tags Tags 09-Jul 0 2,266 0 61 10-Jul 0 2,266 0 61 11-Jul 1 2,267 0 61 12-Jul 1 2,268 0 61 13-Jul 4 2,272 0 61 14-Jul 3 2,275 0 61 15-Jul 2 2,277 0 61 16-Jul 0 2,277 0 61 17-Jul 3 2,280 0 61 18-Jul 0 2,280 0 61 19-Jul 2 2,282 0 61 20-Jul 0 2,282 0 61 21-Jul 1 2,283 0 61 22-Jul 2 2,285 0 61 23-Jul 2 2,287 0 61 24-Jul 2 2,289 0 61 25-Jul 2 2,291 0 61 26-Jul 0 2,291 0 61 27-Jul 1 2,292 0 61 28-Jul 1 2,293 0 61 29-Jul 2 2,295 0 61 30-Jul 3 2,298 0 61 31-Jul 0 2,298 0 61 01-Aug 0 2,298 0 61 02-Aug 0 2,298 0 61 03-Aug 1 2,299 0 61 04-Aug 0 2,299 0 61 05-Aug 0 2,299 0 61 06-Aug 1 2,300 0 61 07-Aug 1 2,301 0 61 08-Aug 1 2,302 0 61 09-Aug 1 2,303 0 61 10-Aug 0 2,303 0 61 11-Aug 0 2,303 0 61 12-Aug 0 2,303 0 61 13-Aug 0 2,303 0 61 14-Aug 0 2,303 0 61 15-Aug 0 2,303 0 61 16-Aug 0 2,303 0 61 17-Aug 1 2,304 0 61 18-Aug 0 2,304 0 61 19-Aug 0 2,304 0 61 20-Aug 0 2,304 0 61 21-Aug 0 2,304 0 61 22-Aug 1 2,305 0 61 Total 2,305 61 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 October 2015 Appendix C: Fixed-station receiver sites (setup and performance) and mobile-tracking survey effort STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 1 October 2015 Table C-1. Location and antenna orientation of fixed-station receivers in the Susitna River drainage, 2014. Site Location Receiver No. Project River Mile Latitude Longitude River Bank Antenna Antenna Orientation Lower Yentna 12 61.66359 -150.62567 Right 1 Downstream Yentna River 2 Upstream Yentna River Skwentna 14 61.87268 -151.35259 Right 1 Downstream Skwentna River 2 Upstream Skwentna River Upper Yentna 17 62.16243 -151.53392 Left 1 Downstream Yentna River 2 Upstream Yentna River Deshka Mouth 8 40 61.69127 -150.30632 Right 1 Downstream Sustina River 2 Upstream Susitna River Sunshine 18 83 62.17300 -150.17428 Left 1 Downstream Sustina River 2 Upstream Susitna River Talkeetna 9 62.34754 -150.01463 Left 1 Downstream Talkeetna River 2 Upstream Talkeetna River Chulitna 29 62.55397 -150.23167 Left 1 Downstream Chulitna River 2 Upstream Chulitna River Deshka Weir 20 61.78585 -150.34572 Right 1 Downstream Deshka River 2 Upstream Deshka River Montana Creek Weir 27 62.10556 -150.04861 Right 1 Downstream Montana Creek 2 Upstream Montana Creek Middle Fork Chulitna Weir 28 63.05900 -149.58222 Left 1 Downstream Middle Fork Chulitna River 2 Upstream Middle Fork Chulitna River Lane Creek 5,10 117 62.52792 -150.11407 Right 1 Downstream Susitna River 2 Upstream Susitna River 3 Across Susitna River Gateway 15 130 62.67645 -149.89303 Right 1 Downstream Susitna River 2 Upstream Susitna River Indian River 25 142 62.78530 -149.65793 Right 1 Downstream Susitna River 2 Upstream Susitna River 3 Up Indian River Cheechako 40 157 62.80794 -149.25392 Left 1 Downstream Susitna River 2 Upstream Susitna River Chinook Creek 45 160 62.80176 -149.16079 Left 1 Downstream Susitna River 2 Upstream Susitna River STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 2 October 2015 Table C-1. Continued. Site Location Receiver No. Project River Mile Latitude Longitude River Bank Antenna Antenna Orientation Devils Island 50 167 62.80926 -149.00268 Mainstem island 1 Downstream Susitna River 2 Upstream Susitna River Watana Dam site 56, 57 187 62.82342 -148.53477 Right 1 Downstream Susitna River 2 Upstream Susitna River Watana Creek 59 197 62.82987 -148.25578 Right 1 Downstream Susitna River 2 Upstream Susitna River 3 Up Watana Creek Kosina Creek 60 209 62.78389 -147.93802 Right 1 Downstream Susitna River 2 Upstream Susitna River 3 Up Kosina Creek Oshetna River 65 235 62.63997 -147.38348 Left 1 Downstream Susitna River 2 Upstream Susitna River 3 Up Oshetna River 1 These stations were located primarily on tributaries; river bank orientations are with respect to the tributary not the Susitna River. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 3 October 2015 Table C-2. Monitoring efficiency (percent operational) of fixed-station receivers in the Lower River Basin in 2014, by week. Week Lower Yentna Upper Yentna Skwentna River Deshka Mouth Deshka Weir Montana Weir Sunshine Mouth Talkeetna Station Chulitna Station Middle Chulitna 4/28 - 5/4 nd nd nd nd nd nd nd nd nd nd 5/5 - 5/11 nd nd nd nd nd nd nd nd nd nd 5/12 - 5/18 nd nd nd nd nd nd nd nd nd nd 5/19 - 5/25 100 nd nd 100 100 nd 100 nd nd nd 5/26 - 6/1 100 100 100 100 100 nd 100 100 nd nd 6/2 - 6/8 100 100 100 100 100 100 100 100 100 nd 6/9 - 6/15 100 100 100 100 100 100 100 100 100 nd 6/16 - 6/22 100 100 100 100 100 100 100 100 100 100 6/23 - 6/29 100 100 100 100 100 100 100 100 100 100 6/30 - 7/6 100 50 100 100 100 100 100 100 100 100 7/7 - 7/13 100 0 100 100 54 100 100 100 100 100 7/14 - 7/20 100 0 100 100 25 100 100 100 100 100 7/21 - 7/27 100 0 100 100 91 100 100 100 100 100 7/28 - 8/3 100 44 100 100 100 100 100 100 100 100 8/4 - 8/10 100 43 100 100 100 100 100 100 100 100 8/11 - 8/17 100 64 100 100 100 100 100 100 100 nd 8/18 - 8/24 100 13 100 100 100 100 100 100 100 nd 8/25 - 8/31 100 12 nd 100 100 100 100 100 100 nd 9/1 - 9/7 100 7 nd 100 100 100 100 100 100 nd 9/8 - 9/14 100 100 nd 100 nd 100 100 100 100 nd 9/15 - 9/21 100 nd nd 100 nd 100 100 100 100 nd 9/22 - 9/28 100 nd nd 100 nd 100 100 100 100 nd 9/29 - 10/5 nd nd nd nd nd nd nd nd nd nd 10/6 - 10/12 nd nd nd nd nd nd nd nd nd nd 10/13 - 10/19 nd nd nd nd nd nd nd nd nd nd 10/20 - 10/26 nd nd nd nd nd nd nd nd nd nd 10/27 - 11/2 nd nd nd nd nd nd nd nd nd nd Notes: Grey: Receiver not scanning; Red: Unknown malfunction. Percentages were calculated as the number of hours of recorded receiver activity divided by the number of hours for which it was deployed, summed by week; "nd" = 'not deployed'. Receivers were considered active in a given hour if at least one fish detection, beacon-tag hit, or noise event was recorded during the hour. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 4 October 2015 Table C-3. Monitoring efficiency (percent operational) of fixed-station receivers in the Middle and Upper River basins in 2014, by week. Week Lane Station (LR Tags) Lane Station (MR Tags)Gateway Indian River Cheechako Chinook Devils Station Watana Dam Site Watana Creek Kosina Creek Oshetna Creek 4/28 - 5/4 nd nd nd 100 nd nd nd nd nd 100 nd 5/5 - 5/11 nd nd nd 100 nd nd nd nd nd 100 nd 5/12 - 5/18 nd nd nd 100 nd nd nd nd nd 94 nd 5/19 - 5/25 100 100 nd 100 nd nd nd nd nd 78 100 5/26 - 6/1 100 100 nd 100 nd nd nd nd nd 100 100 6/2 - 6/8 99 100 nd 100 nd nd nd nd nd 100 100 6/9 - 6/15 100 100 100 100 100 100 34 nd 62 100 100 6/16 - 6/22 100 100 100 100 100 100 93 nd 91 100 100 6/23 - 6/29 100 9 100 100 100 100 99 nd 24 100 100 6/30 - 7/6 100 94 58 100 100 100 88 nd 79 100 100 7/7 - 7/13 100 100 100 100 100 100 100 100 100 100 100 7/14 - 7/20 100 100 100 100 100 100 100 100 100 100 100 7/21 - 7/27 100 100 100 100 100 100 100 100 100 100 100 7/28 - 8/3 100 100 100 100 100 100 74 100 100 100 100 8/4 - 8/10 100 100 100 100 100 100 100 100 100 100 100 8/11 - 8/17 100 100 100 100 100 100 100 100 100 100 100 8/18 - 8/24 100 100 100 100 100 100 100 100 100 100 100 8/25 - 8/31 100 100 100 100 100 100 100 100 100 100 100 9/1 - 9/7 100 100 100 100 100 100 100 30 100 100 100 9/8 - 9/14 100 100 100 100 100 100 100 94 100 100 100 9/15 - 9/21 100 100 100 100 100 100 100 100 100 100 100 9/22 - 9/28 100 100 100 100 100 100 100 100 100 100 100 9/29 - 10/5 100 100 nd 100 100 100 100 100 100 100 100 10/6 - 10/12 nd nd nd 100 100 nd 100 100 100 100 100 10/13 - 10/19 nd nd nd 100 nd nd 100 100 nd 100 nd 10/20 - 10/26 nd nd nd 100 nd nd 100 nd nd nd nd 10/27 - 11/2 nd nd nd 100 nd nd 100 nd nd nd nd Notes: Two receivers were deployed at Lane Station, one to monitor tags released in the lower river ('LR tags') and one for tags released in the middle river ('MR Tags'). Yellow: Low power/dead battery; Grey: Receiver not scanning; Orange: station damaged by wildlife. Percentages were calculated as the number of hours of recorded receiver activity divided by the number of hours for which it was deployed, summed by week; "nd" = 'not deployed'. Receivers were considered active in a given hour if at least one fish detection, beacon-tag hit, or noise event was recorded during the hour. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 5 October 2015 Table C-4. List of the aerial telemetry surveys conducted in 2014, by location, date, and vehicle type (helicopter, fixed-wing). Zone From:To:6-116-126-166-176-186-216-226-246-256-266-286-297-17-27-47-57-77-87-97-107-117-127-13MOB - Little Susitna River -- MOB - Beyond Confluence --H MOB - Confluence - Yentna 3.5 32.4 H H F H H H F H MOB - Yentna River 32.4 -H H H F F H H H F H MOB - Yentna - Deshka 32.4 45.0 H H H F H H H F H MOB - Deshka River 44.9 -H H H F H H H F H MOB - Willow and Little Willow cr 52.2 55.6 H H H F H H H F H MOB - Kashwitna River 64.7 -H H H H H H F H MOB - Deshka - Kashwitna 45.0 64.7 H H H F H H H F H MOB - Caswell Creek 67.4 - MOB - Sheep Creek 70.1 -H H H H F H MOB - Goose Creek 76.9 -F H MOB - Kashwitna - Montana 64.7 80.7 H H H F H H H F H MOB - Montana Creek 80.9 -H H H H H H F H MOB - Montana - Sunshine 80.7 88.5 H H H F H H H F H MOB - Sunshine Creek 88.1 -H H H H MOB - Rabideux Creek 87.4 -H H H H H H H MOB - Birch Creek 93.5 - MOB - Talkeetna River 101.0 -H H H F H H H H F H MOB - Chulitna River 101.7 -H H F H H H H F H MOB - Sunshine - Talkeetna 88.5 102.3 H H H F H H H H H F H MOB - Talkeetna - Lane 102.3 116.7 H H H H H H H H H H MOB - Whiskers Creek 104.8 -H H H H H H H H H MOB - Trib off zone 95 110.5 - MOB - Lane - Gateway 116.7 130.1 H H H H H H H H H H H MOB - Lane Creek 117.1 -H H H H H H MOB - 5th of July Creek 127.3 -H MOB - Slough 8A 129.2 129.8 H H H H H H H MOB - Gateway - 4th of July 130.1 134.3 H H H H H H H H MOB - Slough 9 131.4 133.5 H H H H H H MOB - Sherman Creek 134.1 - PRM Survey Date (m-d) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 6 October 2015 Table C-4. Continued. Zone From:To:6-116-126-166-176-186-216-226-246-256-266-286-297-17-27-47-57-77-87-97-107-117-127-13MOB - 4th of July Creek 134.3 -H H H H H H H H MOB - 4th of July - Slough 11 134.3 140.2 H H H H H H H H MOB - Slough 11 138.6 -H H H H H H H MOB - Gold Creek 140.1 -H H H H H H H H MOB - Slough 11 - Indian 140.1 142.0 H H H H H H H H MOB - Indian trib 141.8 -H H H H H H H H MOB - Indian - Slough 21 142.0 145.7 H H H H H H H H MOB - Slough 21 145.1 145.6 H H H H H H H H MOB - above Powerline 145.7 146.0 H H H H H H H H MOB - abv Powerline - Portage 146.0 152.3 H H H H H H H H H MOB - Jack Long Creek 148.2 -H H H H H MOB - Portage trib 152.3 -H H H H H H H H H H H MOB - Portage - Impediment1 152.3 155.2 H H H H H H H H H H H MOB - Impediment1 - Cheechako 155.2 157.3 H H H H H H H H H H H H MOB - Cheechako Creek 155.9 -H H H H H H H H H H MOB - Cheechako - Impediment2 157.3 160.2 H H H H H H H H H H H MOB - Impediment2 - Chinook 160.2 160.4 H H H H H H H H H H H MOB - Chinook Creek 160.4 -H H H H H H H MOB - Chinook - Impediment3 160.4 164.8 H H H H H H H H H H H MOB - Devils Creek 164.8 -H H H H H H H H H MOB - Impediment3 - Devil Stn 164.8 166.9 H H H H H H H H H H H H MOB - Devil Stn - Fog 166.9 179.4 H H H H H H MOB - Fog Creek 179.3 -H H H H H H MOB - Fog - Dam Site 179.4 187.2 H H H H H H MOB - Tsusena Creek 184.5 -H H H H H H MOB - Dam Site - Deadman 187.2 189.4 H H H H H H MOB - Deadman Creek 189.4 -H H H H H MOB - Deadman - Watana 189.4 196.9 H H H H H H MOB - 'Creek 192'194.8 -H MOB - Watana Creek 196.9 -H H H H H H H MOB - Wantana - Kosina 196.9 209.2 H H H H H H PRM Survey Date (m-d) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 7 October 2015 Table C-4. Continued. Zone From:To:6-116-126-166-176-186-216-226-246-256-266-286-297-17-27-47-57-77-87-97-107-117-127-13MOB - Kosina Creek 209.2 -H H H H H H MOB - Kosina - Jay Creek 209.2 211.0 H H H H H MOB - Jay Creek 211.0 -H H H H H MOB - Jay - Goose 211.0 232.9 H H H H H MOB - Goose Creek (Upper River)232.9 -H H H H H MOB - Goose - Oshetna 232.9 235.1 H H H H H MOB - Oshetna River 235.1 -H H H H H MOB - Oshetna - Tyone 235.1 247.3 H MOB - Tyone River 247.3 -H MOB - Tyone - Clearwater Cr 247.3 266.6 MOB - Clearwater Creek 266.6 - MOB - above Clearwater Cr 266.6 - PRM Survey Date (m-d) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 8 October 2015 Table C-4. Continued. Zone From:To:7-147-167-177-197-207-217-227-237-257-267-287-297-318-18-38-48-58-68-78-88-98-108-11MOB - Little Susitna River -- MOB - Beyond Confluence --H H MOB - Confluence - Yentna 3.5 32.4 H H F H H H F H H MOB - Yentna River 32.4 -H H F H H H H F H F MOB - Yentna - Deshka 32.4 45.0 H H F H H H F H H MOB - Deshka River 44.9 -H H F/H H H F F/H H MOB - Willow and Little Willow cr 52.2 55.6 H H F/H H H F F/H H MOB - Kashwitna River 64.7 -H H F F/H H H F/H H MOB - Deshka - Kashwitna 45.0 64.7 H H F H H H F H H MOB - Caswell Creek 67.4 -H H H H H H MOB - Sheep Creek 70.1 -H H F H H H H F/H H MOB - Goose Creek 76.9 -H H F H H H F/H H MOB - Kashwitna - Montana 64.7 80.7 H H F H H H H F H H MOB - Montana Creek 80.9 -H H F H H H H F/H H MOB - Montana - Sunshine 80.7 88.5 H H F H H H H H F H H MOB - Sunshine Creek 88.1 -H H H H H H H H H MOB - Rabideux Creek 87.4 -H H H H H H H H H H MOB - Birch Creek 93.5 -H H H H H H MOB - Talkeetna River 101.0 -H H F H H H H H H F H H MOB - Chulitna River 101.7 -H H F H H H H H F F/H H MOB - Sunshine - Talkeetna 88.5 102.3 H H F H H H H H H F H H MOB - Talkeetna - Lane 102.3 116.7 H H H H H H H H H H MOB - Whiskers Creek 104.8 -H H H H H H H H H MOB - Trib off zone 95 110.5 -H MOB - Lane - Gateway 116.7 130.1 H H H H H H H H H H H MOB - Lane Creek 117.1 -H H H H H H H H H H MOB - 5th of July Creek 127.3 -H H H MOB - Slough 8A 129.2 129.8 H H H H H H H H H H MOB - Gateway - 4th of July 130.1 134.3 H H H H H H H H H H MOB - Slough 9 131.4 133.5 H H H H H H H H H H MOB - Sherman Creek 134.1 - PRM Survey Date (m-d) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 9 October 2015 Table C-4. Continued. Zone From:To:7-147-167-177-197-207-217-227-237-257-267-287-297-318-18-38-48-58-68-78-88-98-108-11MOB - 4th of July Creek 134.3 -H H H H H H H H H H MOB - 4th of July - Slough 11 134.3 140.2 H H H H H H H H H H MOB - Slough 11 138.6 -H H H H H MOB - Gold Creek 140.1 -H H H H H H H H H H MOB - Slough 11 - Indian 140.1 142.0 H H H H H H H H H H MOB - Indian trib 141.8 -H H H H H H H H H H H MOB - Indian - Slough 21 142.0 145.7 H H H H H H H H H H MOB - Slough 21 145.1 145.6 H H H H H H H H H H MOB - above Powerline 145.7 146.0 H H H H H H H H H H MOB - abv Powerline - Portage 146.0 152.3 H H H H H H H H H H H MOB - Jack Long Creek 148.2 -H H H H H H H H H H MOB - Portage trib 152.3 -H H H H H H H H H H H H MOB - Portage - Impediment1 152.3 155.2 H H H H H H H H H H H H H H H H MOB - Impediment1 - Cheechako 155.2 157.3 H H H H H H H H H H H H H H H H MOB - Cheechako Creek 155.9 -H H H H H H H H H H H H H H H H MOB - Cheechako - Impediment2 157.3 160.2 H H H H H H H H H H H H H H H H MOB - Impediment2 - Chinook 160.2 160.4 H H H H H H H H H H H H H H H H MOB - Chinook Creek 160.4 -H H H H H H H H H H H H H H H H MOB - Chinook - Impediment3 160.4 164.8 H H H H H H H H H H H H H H H H MOB - Devils Creek 164.8 -H H H H H H H H H H H H H H H H MOB - Impediment3 - Devil Stn 164.8 166.9 H H H H H H H H H H H H H H H H MOB - Devil Stn - Fog 166.9 179.4 H H H H H H H MOB - Fog Creek 179.3 -H H H H H MOB - Fog - Dam Site 179.4 187.2 H H H H H H MOB - Tsusena Creek 184.5 -H H H H H MOB - Dam Site - Deadman 187.2 189.4 H H H H H MOB - Deadman Creek 189.4 -H H H H H MOB - Deadman - Watana 189.4 196.9 H H H H H MOB - 'Creek 192'194.8 -H MOB - Watana Creek 196.9 -H H H H H MOB - Wantana - Kosina 196.9 209.2 H H H H H PRM Survey Date (m-d) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 10 October 2015 Table C-4. Continued. Zone From:To:7-147-167-177-197-207-217-227-237-257-267-287-297-318-18-38-48-58-68-78-88-98-108-11MOB - Kosina Creek 209.2 -H H H H MOB - Kosina - Jay Creek 209.2 211.0 H H H MOB - Jay Creek 211.0 -H H H MOB - Jay - Goose 211.0 232.9 H H MOB - Goose Creek (Upper River)232.9 -H H H MOB - Goose - Oshetna 232.9 235.1 H H H MOB - Oshetna River 235.1 -H H MOB - Oshetna - Tyone 235.1 247.3 H MOB - Tyone River 247.3 -H MOB - Tyone - Clearwater Cr 247.3 266.6 H MOB - Clearwater Creek 266.6 -H MOB - above Clearwater Cr 266.6 -H PRM Survey Date (m-d) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 11 October 2015 Table C-4. Continued. Zone From:To:8-128-138-158-168-178-188-198-218-228-248-258-278-288-298-308-319-29-39-49-59-69-89-9MOB - Little Susitna River -- MOB - Beyond Confluence --H H H MOB - Confluence - Yentna 3.5 32.4 H F H H H H F/H H H MOB - Yentna River 32.4 -H F H H H H H F/H H H MOB - Yentna - Deshka 32.4 45.0 H H F H H H H H F/H H H MOB - Deshka River 44.9 -H H F H H H H H F/H H H MOB - Willow and Little Willow cr 52.2 55.6 H H F H H H H H H F H H MOB - Kashwitna River 64.7 -H H F H H H H H H F H H MOB - Deshka - Kashwitna 45.0 64.7 H H F H H H H H F/H H H MOB - Caswell Creek 67.4 -H H H H H H H H H MOB - Sheep Creek 70.1 -H H F H H H H H F H H MOB - Goose Creek 76.9 -H H F H H H H H H F H H MOB - Kashwitna - Montana 64.7 80.7 H H F H H H H H F/H H H MOB - Montana Creek 80.9 -H H F H H H H H H F H H MOB - Montana - Sunshine 80.7 88.5 H H F H H H H H F/H H H MOB - Sunshine Creek 88.1 -H H H H H H H H H H MOB - Rabideux Creek 87.4 -H H H H H H H H H H MOB - Birch Creek 93.5 -H H H H H H H H H H MOB - Talkeetna River 101.0 -H H F F/H H H H H F/H H H MOB - Chulitna River 101.7 -H H F F/H H H H H F H H MOB - Sunshine - Talkeetna 88.5 102.3 H H F H H H H H F/H H H H MOB - Talkeetna - Lane 102.3 116.7 H H H H H H H H H H MOB - Whiskers Creek 104.8 -H H H H H H H H H H MOB - Trib off zone 95 110.5 -H MOB - Lane - Gateway 116.7 130.1 H H H H H H H H H H H H MOB - Lane Creek 117.1 -H H H H H H H H H H MOB - 5th of July Creek 127.3 -H H H H H H MOB - Slough 8A 129.2 129.8 H H H H H H H H H H MOB - Gateway - 4th of July 130.1 134.3 H H H H H H H H H H MOB - Slough 9 131.4 133.5 H H H H H H H H H H MOB - Sherman Creek 134.1 - PRM Survey Date (m-d) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 12 October 2015 Table C-4. Continued. Zone From:To:8-128-138-158-168-178-188-198-218-228-248-258-278-288-298-308-319-29-39-49-59-69-89-9MOB - 4th of July Creek 134.3 -H H H H H H H H H H MOB - 4th of July - Slough 11 134.3 140.2 H H H H H H H H H H MOB - Slough 11 138.6 -H H H H H H H H H H MOB - Gold Creek 140.1 -H H H H H H H H H H MOB - Slough 11 - Indian 140.1 142.0 H H H H H H H H H H MOB - Indian trib 141.8 -H H H H H H H H H H MOB - Indian - Slough 21 142.0 145.7 H H H H H H H H H H MOB - Slough 21 145.1 145.6 H H H H H H H H H H MOB - above Powerline 145.7 146.0 H H H H H H H H H H MOB - abv Powerline - Portage 146.0 152.3 H H H H H H H H H H MOB - Jack Long Creek 148.2 -H H H H H H H H H H MOB - Portage trib 152.3 -H H H H H H H H H H MOB - Portage - Impediment1 152.3 155.2 H H H H H H H H H H MOB - Impediment1 - Cheechako 155.2 157.3 H H H H H H H H H H MOB - Cheechako Creek 155.9 -H H H H H H H H H H MOB - Cheechako - Impediment2 157.3 160.2 H H H H H H H H H H MOB - Impediment2 - Chinook 160.2 160.4 H H H H H H H H H H MOB - Chinook Creek 160.4 -H H H H H H H H H H MOB - Chinook - Impediment3 160.4 164.8 H H H H H H H H H H MOB - Devils Creek 164.8 -H H H H H H H H H H MOB - Impediment3 - Devil Stn 164.8 166.9 H H H H H H H H H H MOB - Devil Stn - Fog 166.9 179.4 H H H H H H H MOB - Fog Creek 179.3 -H H H H H H H MOB - Fog - Dam Site 179.4 187.2 H H H H H H MOB - Tsusena Creek 184.5 -H H H H MOB - Dam Site - Deadman 187.2 189.4 H H H H H MOB - Deadman Creek 189.4 -H H H H H MOB - Deadman - Watana 189.4 196.9 H H H H H MOB - 'Creek 192'194.8 -H H MOB - Watana Creek 196.9 -H H H H H MOB - Wantana - Kosina 196.9 209.2 H H H H H H PRM Survey Date (m-d) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 13 October 2015 Table C-4. Continued. Zone From:To:8-128-138-158-168-178-188-198-218-228-248-258-278-288-298-308-319-29-39-49-59-69-89-9MOB - Kosina Creek 209.2 -H H H H H H MOB - Kosina - Jay Creek 209.2 211.0 H H H H MOB - Jay Creek 211.0 -H H H MOB - Jay - Goose 211.0 232.9 H H H H MOB - Goose Creek (Upper River)232.9 -H H H H MOB - Goose - Oshetna 232.9 235.1 H H H H MOB - Oshetna River 235.1 -H H H H MOB - Oshetna - Tyone 235.1 247.3 H MOB - Tyone River 247.3 - MOB - Tyone - Clearwater Cr 247.3 266.6 H MOB - Clearwater Creek 266.6 - MOB - above Clearwater Cr 266.6 - PRM Survey Date (m-d) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 14 October 2015 Table C-4. Continued. Zone From:To:9-119-149-159-169-179-189-209-219-239-249-259-3010-110-210-710-810-910-1410-1510-1610-1710-2810-2910-30MOB - Little Susitna River -- MOB - Beyond Confluence --H H H MOB - Confluence - Yentna 3.5 32.4 H H H H H H H MOB - Yentna River 32.4 -H H H H H H H MOB - Yentna - Deshka 32.4 45.0 H H H H H H H MOB - Deshka River 44.9 -H H F H H H H H MOB - Willow and Little Willow cr 52.2 55.6 H F H H H H H H MOB - Kashwitna River 64.7 -H F H H H H H H H MOB - Deshka - Kashwitna 45.0 64.7 H F H H H H H H H MOB - Caswell Creek 67.4 -H H H H H H H MOB - Sheep Creek 70.1 -H F H H H H H H H MOB - Goose Creek 76.9 -H F H H H H H H MOB - Kashwitna - Montana 64.7 80.7 H F H H H H H H H MOB - Montana Creek 80.9 -H F H H H H H H H MOB - Montana - Sunshine 80.7 88.5 H H H F H H H H H MOB - Sunshine Creek 88.1 -H H H H H H H H MOB - Rabideux Creek 87.4 -H H H H H H H H MOB - Birch Creek 93.5 -H H H H H H H MOB - Talkeetna River 101.0 -H H H F H H H H H MOB - Chulitna River 101.7 -H H H F H H H H H H MOB - Sunshine - Talkeetna 88.5 102.3 H H H F H H H H H H H MOB - Talkeetna - Lane 102.3 116.7 H H H H H H H H MOB - Whiskers Creek 104.8 -H H H H H H H H H MOB - Trib off zone 95 110.5 - MOB - Lane - Gateway 116.7 130.1 H H H H H H H H H H H MOB - Lane Creek 117.1 -H H H H H H H H H H MOB - 5th of July Creek 127.3 -H H MOB - Slough 8A 129.2 129.8 H H H H H H H H H H H MOB - Gateway - 4th of July 130.1 134.3 H H H H H H H H H H H MOB - Slough 9 131.4 133.5 H H H H H H H H H H H MOB - Sherman Creek 134.1 - PRM Survey Date (m-d) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 15 October 2015 Table C-4. Continued. Zone From:To:9-119-149-159-169-179-189-209-219-239-249-259-3010-110-210-710-810-910-1410-1510-1610-1710-2810-2910-30MOB - 4th of July Creek 134.3 -H H H H H H H H H H MOB - 4th of July - Slough 11 134.3 140.2 H H H H H H H H H H H MOB - Slough 11 138.6 -H H H H H H H H H H H MOB - Gold Creek 140.1 -H H H H H H H H H MOB - Slough 11 - Indian 140.1 142.0 H H H H H H H H H H H MOB - Indian trib 141.8 -H H H H H H H H H H H MOB - Indian - Slough 21 142.0 145.7 H H H H H H H H H H H MOB - Slough 21 145.1 145.6 H H H H H H H H H H H MOB - above Powerline 145.7 146.0 H H H H H H H H H H H MOB - abv Powerline - Portage 146.0 152.3 H H H H H H H H H H H MOB - Jack Long Creek 148.2 -H H H H H H H H H MOB - Portage trib 152.3 -H H H H H H H H H H H MOB - Portage - Impediment1 152.3 155.2 H H H H H H H H H H H H MOB - Impediment1 - Cheechako 155.2 157.3 H H H H H H H H H H H MOB - Cheechako Creek 155.9 -H H H H H H H H H MOB - Cheechako - Impediment2 157.3 160.2 H H H H H H H H H MOB - Impediment2 - Chinook 160.2 160.4 H H H H H H H H H MOB - Chinook Creek 160.4 -H H H H H H H H MOB - Chinook - Impediment3 160.4 164.8 H H H H H H H H H MOB - Devils Creek 164.8 -H H H H H H H H MOB - Impediment3 - Devil Stn 164.8 166.9 H H H H H H H H H MOB - Devil Stn - Fog 166.9 179.4 H H H MOB - Fog Creek 179.3 -H H H MOB - Fog - Dam Site 179.4 187.2 H H H MOB - Tsusena Creek 184.5 -H H H MOB - Dam Site - Deadman 187.2 189.4 H H H MOB - Deadman Creek 189.4 -H H H MOB - Deadman - Watana 189.4 196.9 H H H MOB - 'Creek 192'194.8 -H MOB - Watana Creek 196.9 -H H H MOB - Wantana - Kosina 196.9 209.2 H H H PRM Survey Date (m-d) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix C – Page 16 October 2015 Table C-4. Continued. Zone From:To:9-119-149-159-169-179-189-209-219-239-249-259-3010-110-210-710-810-910-1410-1510-1610-1710-2810-2910-30MOB - Kosina Creek 209.2 -H H H MOB - Kosina - Jay Creek 209.2 211.0 H H H MOB - Jay Creek 211.0 -H H H MOB - Jay - Goose 211.0 232.9 H H H MOB - Goose Creek (Upper River)232.9 -H H H MOB - Goose - Oshetna 232.9 235.1 H H H MOB - Oshetna River 235.1 -H H H MOB - Oshetna - Tyone 235.1 247.3 H H H MOB - Tyone River 247.3 -H H H MOB - Tyone - Clearwater Cr 247.3 266.6 MOB - Clearwater Creek 266.6 - MOB - above Clearwater Cr 266.6 - PRM Survey Date (m-d) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 October 2015 Appendix D: Spawning destinations STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 1 October 2015 Table D-1. Summary of monitoring effort at potential spawning sites, by species, as part of the Habitat Suitability Criteria (HSC) component of the Fish and Aquatics Instream Flow Study (RSP Section 8.5), 2014. Survey Date Presence Spawning Presence Spawning Presence Spawning 11-Aug Slough 10 North Fork Slough 10 East Fork Slough 9A 4th of July Side Channel X 12-Aug Oxbow 1 Unnamed Slough RR (PRM 121.3 ) Slough 9 5th of July Slough 13-Aug 4th of July Side Channel X 4th of July Creek Mouth X X Sherman Creek Mouth Skull Creek Mouth Deadhorse Creek Mouth Oxbow 2 14-Aug Jack Long Creek Mouth Indian River Mouth X X Slough 22 X X 30-Aug Unnammed Slough RL (near PRM 119) Mainstem RL a (near PRM 114) Oxbow 1 Slough 22 X X X X 31-Aug Slough 21 Slough 19 X X X X Slough 16 Slough 15 Slough 10 North Fork X Slough 10 East Fork X X Slough 9A X X Side Channel (PRM 131.5)X X Side Channel(PRM 137)X X Notes: a Sample site located bewteen Gash Creek and Oxbow 1. RL indicates a sampling site located on river left; RR indicates a site on river right. Sockeye Pink Chum STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 2 October 2015 Table D-2. Summary of monitoring effort at potential spawning sites for Chinook Salmon in the Middle River, 2014. Habitat Survey Survey Site #Fish #Location Type Date Type None Presence Holding Spawning 1 681 Lane Creek Tributary Mouth 20-Jul DIDSON X 2a 515 Lane Creek - Gateway Side Channel 20-Jul Boat *Aerial 3 373, 749, 677 5th of July Creek Tributary Mouth *Aerial 4 663 Lane Creek - Gateway Main Channel NA Boat 5 319 4th of July Creek Tributary Mouth 20-Jul DIDSON X 25-Jul DIDSON X 6 809 4th of July Side Channel Side Channel 19-Jul DIDSON X 20-Jul Boat/Visual X 7a 233 Gateway - Slough 11 Main Channel 19-Jul Boat 8a 673 Slough/Side Channel 10 Side Channel 19-Jul Boat 9a 768 Slough/Side Channel 11 Side Channel 19-Jul Boat/Visual 10 291, 634, 708 Gateway - Slough 11 Main Channel 23-Jul DIDSON X 11 695 Gold Creek Tributary Mouth 21-Jul Boat X 12a 335 Slough 11 - Slough 21 Side Channel 23-Jul Boat 13 cluster Indian River Tributary Mouth 22-Jul DIDSON X 14a 714 Slough 11 - Slough 21 Main Channel NA NA 15a 390, 540 Side Channel 21 Side Channel *Boat/Aerial 16 444 Slough 21 - Portage Creek Main Channel NA NA 17 cluster Portage Creek Tributary Mouth 21-Jul DIDSON X 25-Jul DIDSON X 18a 5531 Chinook Creek Tributary Mouth *Aerial a Indicates a site that was visually assessed and considered too difficult to access with boat-mounted DIDSON. * Indicates a site that was monitored on a regular basis, but spawning was not observed or confirmed. Most are turbid water locations. Too turbid to assess Site not surveyed Site not surveyed Site not surveyed Site not visited Too turbid to assess Site not visited Site not surveyed Fish Observed Site not surveyed Too turbid to assess Too turbid to assess Site not visited STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 3 October 2015 Table D-3. Summary of monitoring effort at potential spawning sites for Chum Salmon in the Middle River, 2014. Habitat Survey Survey Site #Fish #Location Type Date Type None Presence Holding Spawning 1 2024 Talkeetna - Lane Creek Main Channel *Aerial 2 2628 Lane Creek - Gateway Side Channel *Aerial 3 2201 5th of July Creek Tributary Mouth 12-Aug HSC X *Aerial X 4 3331 Slough 8D Slough *Aerial X 5 3082 Slough 8A Slough 10-Sep Aerial X X 24-Sep HSC X X 6 1923 Gateway - Slough 11 Side Channel *Aerial/Boat 7 1998, 2120, 2169 Slough 9 Slough 12-Aug HSC X 8 2981 4th of July Creek Tributary Mouth 13-Aug HSC X *Aerial X 9 2078, 3238, 3426 4th of July Side Channel Side Channel 11-Aug HSC X 13-Aug HSC X 10 2929 Slough 11 - Indian River Main Channel *Aerial/Boat 11 2177 Slough 11 - Indian River Main Channel *Aerial/Boat 12 2746 Indian River Tributary Mouth 14-Aug HSC X 10-Sep Aerial X X 13 3094 Indian River - Slough 21 Main Channel *Aerial/Boat 14 2329 Portage Creek Tributary Mouth *Aerial X * Indicates a site that was monitored on a regular basis, but spawning was not observed or confirmed. Most are turbid water locations. Too turbid to assess Fish Observed Too turbid to assess Too turbid to assess Too turbid to assess Too turbid to assess Too turbid to assess STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 4 October 2015 Table D-4. Summary of monitoring effort at potential spawning sites for Coho Salmon in the Middle River, 2014. Habitat Survey Survey Site #Fish #Location Type Date Type None Presence Holding Spawning 1a 3639 Lane Creek - Gateway Main Channel *Aerial 2 2901 Oxbow 2 Side Channel *Aerial/Boat 3 2932, 3371 Gateway - Slough 11 Side Channel *Aerial X 4 1830 Slough 13 Slough *Aerial X 5 1881 Slough 11 - Indian River Main Channel *Aerial X 6 3370, 3398 Indian River Tributary Mouth *Aerial X 7 3396 Slough 22 Slough 30-Aug HSC X 8 1960 Portage Creek Tributary Mouth 10-Sep Aerial X a Indicates a site that was visually assessed and not considered suitable for spawning based on physical characteristics * Indicates a site that was monitored on a regular basis, but spawning was not observed or confirmed. Most are turbid water locations. Fish Observed Too turbid to assess Too turbid to assess STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 5 October 2015 Table D-5. Summary of monitoring effort at potential spawning sites for Pink Salmon in the Middle River, 2014. Habitat Survey Survey Site #Fish #Location Type Date Type None Presence Holding Spawning 1 1709, 1856 Side Channel 6A Side Channel *Boat/Aerial 2 2019 5th of July Creek Tributary Mouth 12-Aug HSC X 3 1663, 1664, 2926 4th of July Creek Tributary Mouth 11-Aug HSC X X 13-Aug HSC X X 4 2432 4th of July Side Channel Side Channel 11-Aug HSC X 13-Aug HSC X 5 2423 Gateway - Slough 11 Main Channel *Boat/Aerial 6 1537 Slough 11 - Slough 21 Side Channel *Boat/Aerial 7 1300, 2033 Indian River Tributary Mouth 14-Aug HSC X X 8 2198 Side Channel 21 Side Channel *Boat/Aerial * Indicates a site that was monitored on a regular basis, but spawning was not observed or confirmed. Most are turbid water locations. Fish Observed Too turbid to assess Too turbid to assess Too turbid to assess Too turbid to assess STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 6 October 2015 Table D-6. Summary of monitoring effort at potential spawning sites for Sockeye Salmon in the Middle River, 2014. Habitat Survey Survey Site #Fish #Location Type Date Type None Presence Holding Spawning 1 2056 Slough 8D Slough *Aerial X 2 cluster Slough 8A Slough 10-Sep Aerial X X 24-Sep HSC X X 3 cluster Slough 9 Slough 12-Aug HSC X 4 1392, 1914, 2076 4th of July Side Channel Side Channel 11-Aug HSC X 13-Aug HSC X 5 cluster Slough 10 Slough 11-Aug HSC X 31-Aug HSC X X 23-Sep HSC X X 6 cluster Slough 11 Slough 10-Sep Aerial X X 21-Sep HSC X X 7 cluster Slough/Side Channel 21 Slough/SideChannel 30-Aug Aerial X X 31-Aug HSC X 10-Sep Aerial X X 22-Sep HSC X X 8 cluster Slough 22 Slough 14-Aug HSC X 30-Aug HSC X X b Indicates a site that was visually assessed and not considered suitable for spawning based on physical characteristics * Indicates a site that was monitored on a regular basis, but spawning was not observed or confirmed. Most are turbid water locations. Fish Observed STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 7 October 2015 Table D-7. Details of impediment-passage events for radio-tagged fish, 2014. Chinook Salmon (≥ 50 cm) that Passed Impediment 3 Tag Number First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Hold Time Below I1 (d) Hold Time Below I2 (d) Hold Time Below I3 (d) Flow at I-1 Passage (cfs) Flow at I-2 Passage (cfs) Flow at I-3 Passage (cfs) 537 20 Jul 20 Jul 4 Aug 4.5 0.5 8.0 21,400 21,400 16,400 787 20 Jul 20 Jul 30 Jul 2.5 0.5 5.5 21,400 21,400 15,900 Average 20 Jul 20 Jul 2 Aug 3.5 0.5 6.8 21,400 21,400 16,150 Chinook Salmon (≥ 50 cm) That Passed Impediment 2 but not Impediment 3 Tag Number First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Hold Time Below I1 (d) Hold Time Below I2 (d) Hold Time Below I3 (d) Flow at I-1 Passage (cfs) Flow at I-2 Passage (cfs) Flow at I-3 Passage (cfs) 17 30 Jun 30 Jun -1.0 0.5 4.5 19,600 19,600 - 139 24 Jul 28 Jul -7.0 1.0 d.n.a.18,100 16,800 - 222 6 Jul 18 Jul -1.0 0.5 17.0 23,800 19,000 - 516 1 Aug 1 Aug -10.5 0.5 d.n.a.16,000 16,000 - 882 25 Jul 1 Aug -4.5 0.5 d.n.a.17,900 16,000 - 903 23 Jul 24 Jul -0.5 0.5 10.5 18,100 18,100 - 5531 18 Jul 18 Jul -2.5 0.5 13.0 19,000 19,000 - Average 18 Jul 22 Jul 3.9 0.6 11.3 18,929 17,786 Chinook Salmon (≥ 50 cm) That Passed Impediment 1 but not Impediment 2 Tag Number First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Hold Time Below I1 (d) Hold Time Below I2 (d) Hold Time Below I3 (d) Flow at I-1 Passage (cfs) Flow at I-2 Passage (cfs) Flow at I-3 Passage (cfs) 221 20 Jul --4.5 d.n.a.-21,400 -- 828 18 Jul --2.5 d.n.a.-19,000 -- 868 23 Jul --5.5 d.n.a.-18,100 -- 5702 1 Jul --3.0 d.n.a.-23,300 -- Average 16 Jul 3.9 -20,450 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 8 October 2015 Table D-7. Continued. Chinook Salmon (≥ 50 cm) That Approached Impediment 1 but did not Pass Tag Number First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Hold Time Below I1 (d) Hold Time Below I2 (d) Hold Time Below I3 (d) Flow at I-1 Passage (cfs) Flow at I-2 Passage (cfs) Flow at I-3 Passage (cfs) 23 ---3.0 ----- 33 ---4.5 ----- 40 ---1.5 ----- 91 ---4.5 ----- 103 ---25.5 ----- 108 ---5.0 ----- 111 ---4.5 ----- 166 ---1.5 ----- 198 ---1.5 ----- 237 ---4.0 ----- 239 ---1.5 ----- 244 ---13.5 ----- 264 ---7.5 ----- 300 ---2.5 ----- 359 ---1.5 ----- 562 ---1.5 ----- 611 ---2.5 ----- 621 ---12.0 ----- 668 ---0.5 ----- 716 ---0.5 ----- 818 ---1.0 ----- 5242 ---2.0 ----- 5255 ---3.0 ----- 5384 ---5.5 ----- 5408 ---1.5 ----- Average 4.5 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 9 October 2015 Table D-7. Continued. Chinook Salmon (< 50 cm) That Approached Impediment 1 but did not Pass Tag Number First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Hold Time Below I1 (d) Hold Time Below I2 (d) Hold Time Below I3 (d) Flow at I-1 Passage (cfs) Flow at I-2 Passage (cfs) Flow at I-3 Passage (cfs) 574 ---1.5 ----- Average -1.5 - Sockeye Salmon That Passed Impediment 1 but not Impediment 2 Tag Number First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Hold Time Below I1 (d) Hold Time Below I2 (d) Hold Time Below I3 (d) Flow at I-1 Passage (cfs) Flow at I-2 Passage (cfs) Flow at I-3 Passage (cfs) 785 27 Jul --0.5 d.n.a.-18,900 -- 955 8 Sep --1.0 d.n.a.-14,400 -- 2214 8 Sep --3.0 d.n.a.-14,400 -- Average 25 Aug 1.5 -15,900 Sockeye Salmon That Approached Impediment 1 but did not Pass Tag Number First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Hold Time Below I1 (d) Hold Time Below I2 (d) Hold Time Below I3 (d) Flow at I-1 Passage (cfs) Flow at I-2 Passage (cfs) Flow at I-3 Passage (cfs) 647 ---1.5 ----- 837 ---1.5 ----- 1053 ---1.5 ----- 1260 ---20.5 ----- 1293 ---2.5 ----- 1346 ---1.5 ----- 1404 ---1.5 ----- 1851 ---3.0 ----- 2076 ---3.5 ----- 2156 ---1.0 ----- 2157 ---10.5 ----- 2606 ---5.5 ----- STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 10 October 2015 Table D-7. Continued. Sockeye Salmon That Approached Impediment 1 but did not Pass Tag Number First Detection Above I-1 First Detection Above I-2 First Detection Above I-3 Hold Time Below I1 (d) Hold Time Below I2 (d) Hold Time Below I3 (d) Flow at I-1 Passage (cfs) Flow at I-2 Passage (cfs) Flow at I-3 Passage (cfs) 2770 ---7.0 ----- Average 4.7 Notes: Details include the date of first detections above each impediment, the duration of holding time below each impediment, and the flow (measured at Tsusena Creek) at the time of the first detection upstream of the impediment. "d.n.a" = Did Not Approach next upstream impediment. Top panel: Chinook salmon (≥ 50 cm) that passed Impediment 3. Second panel: Chinook salmon (≥ 50 cm) that passed Impediment 2, but not Impediment 3. Third panel: Chinook salmon (≥ 50 cm) that passed Impediment 1, but not Impediment 2. Fouth panel: Chinook salmon (≥ 50 cm) that approached within 1 km of Impediment 1, but did not pass. Fifth panel: Chinook salmon (< 50 cm) that approached within 1 km of Impediment 1, but did not pass. Sixth panel: Sockeye salmon that passed Impediment One, but not Impediment Two. Seventh panel: Sockeye salmon that approached within 1 km of Impediment 1, but did not pass. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 11 October 2015 Table D-8. Number of Chinook Salmon counted during aerial spawner surveys, by location and survey period, 2014. River Section Waterbody Miles Surveyed Jul 14 - Jul 15 Jul 19 - Jul 20 Jul 25 - Jul 26 Jul 31 - Aug 1 Aug 6 - Aug 7 Aug 12 - Aug 13 Aug 18- Aug 19 Between Impediment 1 & 2 Cheechako Creek Susitna 155.9 2.4 11 16 8 13 7 0 0 Between Impediment 2 & 3 Chinook Creek Susitna 160.4 8.7 0 5 5 2 2 0 0 Middle River -Devil Creek Susitna 164.8 2.5 0 0 0 2 10 5 2 Above Impediment 3 Fog Creek Susitna 179.3 19.3 0 0 0 3 2 0 1 Fog Creek Tributary L1 Fog 5.1 7.6 0 0 0 0 0 0 0 Bear Creek Susitna 184.0 5.7 0 0 0 0 0 0 0 Bear Creek Tributary R1 Bear 0.8 8.2 0 0 0 0 0 0 0 Tsusena Creek Susitna 184.4 3.6 0 0 0 0 0 0 0 Upper River -Deadman Creek Susitna 188.4 0.3 0 0 0 0 0 0 0 Within Reservoir Watana Creek Susitna 196.9 21.3 0 0 0 0 0 0 0 Watana Creek Tributary R5 Watana 8.6 8.6 0 0 0 0 0 0 0 Kosina Creek Susitna 209.2 18.8 0 0 0 0 0 0 0 Gilbert Creek Kosina 6.2 6 0 NS2 NS2 0 0 0 0 Tsisi Creek Kosina 7.3 6.4 0 NS2 0 0 0 0 NS1 Tsisi Lake 1 Tsisi 7.2 2.8 NS1 NS1 0 0 0 0 0 Tsisi Lake 2 Tsisi 10.6 5.2 NS1 NS1 0 0 0 0 0 Jay Creek Susitna 211.0 13.3 0 0 0 0 0 0 0 Upper River -Goose Creek Susitna 232.9 11.2 0 0 0 0 0 0 0 Above Reservoir Oshetna River Susitna 235.1 26.3 0 0 0 0 NS2 0 0 Black River Oshetna 6.2 0 0 0 0 NS2 0 1 No survey - surveys targeting Sockeye Salmon began July 25-26. 2 No survey - high and/or turbid water prevented survey. Confluence Project River Mile Survey Dates STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 12 October 2015 Table D-9. Summary of weather variability during the adult salmon aerial spawner surveys in the Middle and Upper rivers, 2014. Table D-10. Summary of survey condition rankings during the adult salmon aerial spawner surveys in the Middle and Upper rivers, 2014. Date Sunny Partly Cloudy Overcast Showers Rain Wind July 14-15 X X X X July 19-20 X X X July 25-26 X X X X X July 31-Aug 1 X X X X Aug 6-7 X X X Aug 12-13 X X Aug 18-19 X X X X Weather Condition Variable Average Rank Observed Range Standard Deviation Sun Glare 4 3 to 4 0.5 Water clarity 3 0 to 4 0.8 Vegetation Cover 3 1 to 4 0.8 Notes: Variables were ranked from 0 to 4, with 4 being optimal and 0 being poor. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 13 October 2015 Figure D-1. Destinations for radio-tagged Chinook Salmon released in the Lower River in 2012-2014. Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination. Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 14 October 2015 Figure D-2. Destinations for radio-tagged Chinook Salmon released in the Middle River in 2012-2014. Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 15 October 2015 Figure D-3. Destinations for radio-tagged Chum Salmon released in the Lower River in 2012. Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 16 October 2015 Figure D-4. Destinations for radio-tagged Chum Salmon released in the Middle River in 2012-2014. Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 17 October 2015 Figure D-5. Destinations for radio-tagged Coho Salmon released in the Lower River in 2012-2014. Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 18 October 2015 Figure D-6. Destinations for radio-tagged Coho Salmon released in the Middle River in 2012-2014. Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 19 October 2015 Figure D-7. Destinations for radio-tagged Pink Salmon released in the Lower River in 2012-2014. Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 20 October 2015 Figure D-8. Destinations for radio-tagged Pink Salmon released in the Middle River in 2012-2014. Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 21 October 2015 Figure D-9. Destinations for radio-tagged Sockeye Salmon released in the Lower River in 2012. Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 22 October 2015 Figure D-10. Destinations for radio-tagged Sockeye Salmon released in the Middle River in 2012-2014. Proportions classified to tributary destinations are shown in yellow circles. The proportion classified to any mainstem destination is shown in an arbitrarily-placed pink circle. In the green circle shows the proportion of fish that were tracked but that could not be conclusively assigned to a destination Proportions are calculated from the total numbers of tags released, after excluding fish with one or fewer detections, that never moved, or moved only downstream. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 23 October 2015 Figure D-11. Potential mainstem spawning sites for radio-tagged Chinook Salmon in the Lower River, PRM 40–104, 2012-2014. Red dots indicate locations of individual radio-tagged fish. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 24 October 2015 Figure D-12. Potential mainstem spawning sites for radio-tagged Chinook Salmon in the Middle River (red and yellow dots), PRM 103–157, 2012 - 2014. Colored dots (red, yellow, or green) indicate individual radio- tagged fish. Green dots also include locations that did not have a radio-tagged fish, but spawning was confirmed during opportunistic surveys. Black dots indicate spawning locations by project river mile confirmed during historic surveys (summarized in Barrett et al. 1985a,b and Thompson et al. 1986). Multiple green dots within an inset indicate a location that was confirmed for spawning and not each individual fish. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 25 October 2015 Figure D-13. Potential mainstem spawning sites for radio-tagged Chum Salmon in the Lower River, PRM 103–157, 2012 - 2014. Colored dots (red, yellow, or green) individual radio-tagged fish. Black dots indicate spawning locations confirmed during historic surveys (summarized in Barrett et al. 1985a,b and Thompson et al. 1986). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 26 October 2015 Figure D-14. Potential mainstem spawning sites for radio-tagged Chum Salmon in the northern half of the Middle River, PRM 40–104, 2012 - 2014. Colored dots (red, yellow, or green) indicate individual radio- tagged fish. Green dots also include locations that did not have a radio-tagged fish, but spawning was confirmed during opportunistic surveys. Black dots indicate spawning locations by project river mile confirmed during historic surveys (summarized in Barrett et al. 1985a,b and Thompson et al. 1986). Multiple green dots within an inset indicate a location that was confirmed for spawning and not each individual fish. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 27 October 2015 Figure D-15. Potential mainstem spawning sites for radio-tagged Chum Salmon in the southern half of the Middle River, PRM 103–157, 2012 -2014. Colored dots (red, yellow, or green) indicate individual radio- tagged fish. Green dots also include locations that did not have a radio-tagged fish, but spawning was confirmed during opportunistic surveys. Black dots indicate spawning locations by project river mile confirmed during historic surveys (summarized in Barrett et al. 1985a,b and Thompson et al. 1986). Multiple green dots within an inset indicate a location that was confirmed for spawning and not each individual fish. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 28 October 2015 Figure D-16. Potential mainstem spawning sites for radio-tagged Coho Salmon in the Lower River, PRM 40– 104, 2012 - 2014. Red dots represent individual radio-tagged fish. Black dots indicate spawning locations confirmed during historic surveys (summarized in Barrett et al. 1985a,b and Thompson et al. 1986). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 29 October 2015 Figure D-17. Potential mainstem spawning sites for radio-tagged Coho Salmon in the Middle River, PRM 103–157, 2012 - 2014. Colored dots (red, yellow, or green) indicate individual radio-tagged fish. Green dots also include locations that did not have a radio-tagged fish, but spawning was confirmed during opportunistic surveys. Black dots indicate spawning locations by project river mile confirmed during historic surveys (summarized in Barrett et al. 1985a,b and Thompson et al. 1986). Multiple green dots within an inset indicate a location that was confirmed for spawning and not each individual fish. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 30 October 2015 Figure D-18. Potential mainstem spawning sites for radio-tagged Pink Salmon in the Lower River, PRM103– 157, 2012 -2014. Red dots represent individual radio-tagged fish. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 31 October 2015 Figure D-19. Potential mainstem spawning sites for radio-tagged Pink Salmon in the Middle River, PRM 103–157, 2012 - 2014. Colored dots (red, yellow, or green) indicate individual radio-tagged fish. Green dots also include locations that did not have a radio-tagged fish, but spawning was confirmed during opportunistic surveys. Black dots indicate spawning locations by project river mile confirmed during historic surveys (summarized in Barrett et al. 1985a,b and Thompson et al. 1986). Multiple green dots within an inset indicate a location that was confirmed for spawning and not each individual fish. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix D – Page 32 October 2015 Figure D-20. Potential mainstem spawning sites for radio-tagged Sockeye Salmon in the Middle River, PRM 103–157, 2012 - 2014. Colored dots (red, yellow, or green) indicate individual radio-tagged fish. Green dots also include locations that did not have a radio-tagged fish, but spawning was confirmed during opportunistic surveys. Black dots indicate spawning locations by project river mile confirmed during historic surveys (summarized in Barrett et al. 1985a,b and Thompson et al. 1986). Multiple green dots within an inset indicate a location that was confirmed for spawning and not each individual fish. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 October 2015 Appendix E: Radio Tag Recoveries STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix E – Page 1 October 2015 Table E-1. Radio tag recovery information for fish released in the Lower River and Yentna River, 2014. Species Tag Date Recovery (Release Location)Number Recovered Description Latitude Longitude Method Chinook Salmon,5245 23 Jun Clear Cr., Talkeetna trib.62.3799 -150.0162 angler METF ≥ 50 cm 5285 22 Jun Deshka River mouth 61.6976 -150.3173 angler (Lower River)5337 30 Jun Clear Creek (Chunilna), river mile 1.75 62.3799 -150.0162 angler 5340 14 Jun Deshka River 61.7294 -150.3192 angler 5374 18 Jun Deshka River mouth 61.6976 -150.3173 angler 5378 18 Jun Deshka River 61.7294 -150.3192 angler 5402 31 May Deshka River mouth 61.6976 -150.3173 angler 5416 4 Jun Deshka River mouth 61.6976 -150.3173 angler 5476 16 Jun Deshka River mouth 61.6976 -150.3173 angler 5492 1 Jul Deshka River, river mile 17 61.7886 -150.3398 angler 5494 30 Jun Lake Creek mouth 61.9092 -150.9096 angler 5552 15 Jun Susitna R., 3/4 mi. below Montana Cr.62.0720 -150.1022 angler 5563 11 Aug Peters Creek, 8mi. upstream from Petersville bridge 62.4611 -150.7463 angler 5566 5 Aug Middle Fork Chulitna River, by Parks Highway crossing 63.2553 -149.2454 angler 5686 14 Jul Sheep Creek mouth 61.9712 -150.0875 angler 5693 1 Jul Deshka River, 3 miles up 61.7418 -150.3119 angler 5755 14 Jun Deshka River above river mile 7 61.7886 -150.3398 angler 5757 13 Jun Deshka River mouth 61.6976 -150.3173 angler 5774 14 Jun Deshka River mouth 61.6976 -150.3173 angler 5871 14 Jun Deshka River mouth 61.6976 -150.3173 angler 5876 14 Jun Deshka River 61.7294 -150.3192 angler 5881 22 Jun Deshka River, 3/4mi. below Laub's homestead 61.7475 -150.3215 angler 5889 1 Aug Deception Creek, at Four Mile Road 61.7479 -149.9343 field crew 5893 25 Jun Deshka River, 2 mi. upstream 61.7294 -150.3192 angler Chinook Salmon,6513 28 Jul Lake Creek 61.9307 -150.9127 angler METF ≥ 50 cm 6696 11 Jul Lake Cr. about 1 mi. upstream from Yentna 61.9307 -150.9127 angler (Yentna River)6724 20 Jun Yentna River, mouth of Lake Cr.61.9092 -150.9096 angler 6753 16 Jun Yentna River, mouth of Lake Creek, McDougal Slough 61.9092 -150.9096 angler Recovery Location 1 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix E – Page 2 October 2015 Table E-1. Continued. Species Tag Date Recovery (Release Location)Number Recovered Description Latitude Longitude Method Coho Salmon 5298 30 Jul Little Willow Creek 61.8101 -150.1020 angler (Lower River)5300 1 Aug Deshka River mouth 61.6976 -150.3173 angler 5398 21 Aug Talkeetna River 62.3393 -150.1042 angler 5946 11 Aug Sunshine Creek 62.1782 -150.1032 angler 5983 24 Aug Trapper Creek [assume at the mouth]62.2551 -150.1723 angler 5985 8 Aug Deshka mouth 61.6976 -150.3173 angler 6031 22 Aug Talkeetna River, foot of Mainstem 62.3287 -150.1136 angler 6055 9 Aug Talkeetna River, 2.5 miles upstream 62.3484 -150.0578 angler 6105 9 Aug Talkeenta River 62.3393 -150.1042 angler 6108 18 Jul Deshka River mouth 61.6976 -150.3173 angler 6127 1 Aug Deshka River 61.7294 -150.3192 angler 6137 4 Aug Willow Creek mouth 61.7833 -150.1671 angler 6140 6 Aug Kashwitna River, 0.25 up from mouth 61.9146 -150.0964 angler 6156 24 Aug Trapper Creek [assume at the mouth]62.2531 -150.1680 angler 6157 16 Aug Rabideux Creek mouth 62.1750 -150.1933 angler 6182 16 Aug Clear Creek 62.3799 -150.0162 angler 6202 4 Aug Whiskers Creek 62.3770 -150.1720 angler 6210 15 Aug Deshka River (Moose Creek at Oilwell Rd.)61.7538 -150.3265 angler 6213 15 Aug Clear Creek 62.3799 -150.0162 angler 6244 6 Aug Deshka mouth 61.6976 -150.3173 angler 6253 21 Aug Talkeetna River, ~2 miles up 62.3463 -150.0754 angler 6260 10 Aug Clear Creek mouth (Chunilna)62.3799 -150.0162 angler 6260 31 Aug Clear Creek (Talkeenta River)62.3799 -150.0162 angler 6264 1 Aug Deshka River mouth 61.6976 -150.3173 angler 6285 27 Aug Kashwitna River, 0.5 mile up from RR Bridge 61.9383 -150.0455 angler 6296 15 Aug Willow Creek mouth 61.7833 -150.1671 angler 6341 11 Aug Montana Creek mouth 62.1045 -150.0757 angler 6393 23 Aug Talachulitna, Mouth 61.8658 -151.4143 angler 6413 20 Aug Troublesome Creek, Mouth 62.6546 -150.2399 angler 6420 15 Aug Deshka River, about 1 mile upstream 61.7294 -150.3192 angler Recovery Location 1 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix E – Page 3 October 2015 Table E-1. Continued. Species Tag Date Recovery (Release Location)Number Recovered Description Latitude Longitude Method Coho Salmon 6429 16 Aug Sunshine Creek, 100 yards above Susitna confluence 62.1782 -150.1032 angler (Lower River)6459 31 Jul Deshka River 61.7294 -150.3192 field crew 6488 17 Aug Little Willow mouth 61.7986 -150.1519 angler 6489 17 Aug Montana Creek mouth 62.1045 -150.0757 angler 6495 19 Aug Sheep Creek 61.9915 -150.0725 angler 6498 29 Aug Talkeetna River 62.3393 -150.1042 angler Coho Salmon 6806 24 Aug Deshka River, ~4 miles up 61.7538 -150.3265 other (Yentna River)6851 6 Aug Deshka weir 61.7856 -150.3450 field crew Pink Salmon 5058 6 Aug Willow Creek, downstream of Deception Creek mouth 61.7788 -150.1259 angler (Lower River)5174 30 Jul Montana Creek 62.1056 -150.0486 angler 1 Recovery coordinates were estimated based on the site description; radio tag recoveries only. Recovery Location 1 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix E – Page 4 October 2015 Table E-2. Radio tag recovery information for fish released in the Middle River, 2014. Tag Date Recovery Species Number Recovered Description Latitude Longitude Method Chinook Salmon 48 16 Jul Indian River 62.8263 -149.6484 other (METF ≥ 50 cm)48 16 Jul Indian River 62.8263 -149.6484 other 160 19 Aug Indian River (mile 266.7 on RR)62.8394 -149.6435 other 245 3 Jul Susitna River at Indian 62.7845 -149.6596 angler 297 7 Aug Twin Bridges South of Curry 62.8394 -149.6435 angler 333 1 Jul LGL Fishwheel 3 62.6374 -149.9775 field crew 388 2 Jul LGL Fishwheel 3 62.6374 -149.9775 field crew 459 25 Aug LGL Fishwheel 3 62.6374 -149.9775 field crew 564 29 Jul LGL Fishwheel 3 62.6374 -149.9775 field crew 590 29 Jul LGL Fishwheel 3 62.6374 -149.9775 field crew 739 22 Jul LGL Fishwheel 3 62.6374 -149.9775 field crew 806 3 Oct Mouth of Jack Long 62.8227 -149.4976 other 812 10 Aug Indian River mouth 62.7845 -149.6596 angler Chum Salmon 1680 4 Sep 4th of July Mouth 62.7156 -149.8053 field crew Coho Salmon 2130 22 Aug Portage Creek 62.8384 -149.3741 angler 2810 27 Aug Talkeetna River, just below Clear Creek 62.3799 -150.0162 angler Pink Salmon 1664 4 Sep 4th of July Mouth 62.7156 -149.8053 field crew 2073 20 Aug Montana Creek Weir 62.1056 -150.0486 field crew Sockeye Salmon 1346 26 Sep in a slough at PRM141 62.7832 -149.6696 other 1927 12 Oct RM 145, ouside Slough 21 62.8137 -149.5802 other 1950 7 Aug Larson Creek 62.3708 -149.8563 angler 2330 10 Oct Chase Creek 62.4447 -150.1321 other 2609 11 Sep Disappointment Creek (trib to Talkeetna R)62.4556 -149.6672 other 1 Recovery coordinates were estimated based on the site description Recovery Location 1 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 October 2015 Appendix F: Tracking histories of chinook salmon above Impediment 3 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 1 October 2015 Table F-1. Summary of migration and spawning behavior for radio-tagged Chinook Salmon after they passed Impediment 3, 2012–2014. Max Max Max Max Total Tag Capture Length Spawning First Last Upstream Upstream Downstream Downstream Live Number Date (cm)Sex Area Live Live Days Location Distance Days Location Distance Days Days1 2012 27 22 Jun 78 TL Undetermined Chinook 28 Jul 5 Aug 8 Kosina Mouth 80 11 Curry 60 8 26 52 25 Jun 89 TL Undetermined Kosina 20 Jul 9 Aug 20 ------28 94 29 Jun 81 TL Undetermined Devil 23 Jul 5 Aug 13 Fog 30 4 Cheechako 19 12 31 104 29 Jun 66 TL Undetermined Portage 24 Jul 30 Jul 6 Above Devil Creek 30 10 ---10 113 30 Jun 84 TL Undetermined Kosina 26 Jul 7 Aug 12 ------19 219 2 Jul 73 TL Male Kosina 23 Jul 26 Jul 3 Above Kosina 30 1 ---7 246 3 Jul 85 TL Female Kosina 23 Jul 26 Jul 3 ------6 257 3 Jul 89 TL Female Portage 30 Jul 17 Aug 18 Devil Creek 24 13 ---28 266 4 Jul 101 TL Male Portage 24 Jul 6 Aug 13 Near Fog 44 15 ---19 359 6 Jul 93 TL Male Portage 6 Aug 11 Aug 5 Kosina 93 26 ---25 5005 26 May -Undetermined Kosina 23 Jul 31 Jul 8 ---Portage 103 16 30 5019 28 May 87 MEF Undetermined Kosina 23 Jul 11 Aug 19 ------24 2013 241 21 Jun 64 MEF Undetermined Unknown -30 Aug -Near headwater --Below Talkeetna --45 272 23 Jun 64 MEF Undetermined Devils 30 Jul 12 Aug 14 Devils Creek 0 0 Devils Creek 0 0 13 395 26 Jun 65 MEF Undetermined Tsesena 22 Jul 1 Aug 11 near Deadman Creek 6.5 1 Tsusena Creek 0 0 19 2014 537 4 Jul 80 MEF Male Unknown -9 Aug -Just Above I-3 -----36 787 11 Jul 78 MEF Undetermined Kosina 2 Aug 18 Aug *17 *Oshetna 40 5 ---20 * * Motion sensor malfunctioned. Mortality date is approximate. 1 Total days the fish was alive after passing Impediment 3 (accounts for the 1 day that tags must be motionless before going into mortality mode). Notes: Distances are in kilometers (1 km = 0.62 mi) Spawning Period Explorations Before Spawning Downstream After Spawning STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 2 October 2015 Figure F-1. Tracking history of a radio-tagged Chinook Salmon (tag #537) that was detected above Impediment 3, PRM 123–167, 2014. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 3 October 2015 Figure F-2. Tracking history of a radio-tagged Chinook Salmon (tag #787) that was detected above Impediment 3, PRM 97–245, 2014. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 4 October 2015 Figure F-3. Tracking history of a radio-tagged Chinook Salmon (tag #27) that was detected above Impediment 3, 2012. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 5 October 2015 Figure F-4. Tracking history of a radio-tagged Chinook Salmon (tag #52) that was detected above Impediment 3, 2012. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 6 October 2015 Figure F-5. Tracking history of a radio-tagged Chinook Salmon (tag #94) that was detected above Impediment 3, 2012. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 7 October 2015 Figure F-6. Tracking history of a radio-tagged Chinook Salmon (tag #104) that was detected above Impediment 3, 2012. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 8 October 2015 Figure F-7. Tracking history of a radio-tagged Chinook Salmon (tag #113) that was detected above Impediment 3, 2012. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 9 October 2015 Figure F-8. Tracking history of a radio-tagged Chinook Salmon (tag #219) that was detected above Impediment 3, 2012. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 10 October 2015 Figure F-9. Tracking history of a radio-tagged Chinook Salmon (tag #246) that was detected above Impediment 3, 2012. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 11 October 2015 Figure F-10. Tracking history of a radio-tagged Chinook Salmon (tag #257) that was detected above Impediment 3, 2012. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 12 October 2015 Figure F-11. Tracking history of a radio-tagged Chinook Salmon (tag #266) that was detected above Impediment 3, 2012. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 13 October 2015 Figure F-12. Tracking history of a radio-tagged Chinook Salmon (tag #359) that was detected above Impediment 3, 2012. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 14 October 2015 Figure F-13. Tracking history of a radio-tagged Chinook Salmon (tag #5005) that was detected above Impediment 3, 2012. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 15 October 2015 Figure F-14. Tracking history of a radio-tagged Chinook Salmon (tag #5019) that was detected above Impediment 3, 2012. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 16 October 2015 Figure F-15. Tracking history of a radio-tagged Chinook Salmon (tag #241) that was detected above Impediment 3, 2013. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 17 October 2015 Figure F-16. Tracking history of a radio-tagged Chinook Salmon (tag #272) that was detected above Impediment 3, 2013. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix F – Page 18 October 2015 Figure F-17. Tracking history of a radio-tagged Chinook Salmon (tag #395) that was detected above Impediment 3, 2013. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 October 2015 Appendix G: Counts of chinook salmon at Watana Canyon using sonar STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 1 October 2015 1. INTRODUCTION The Alaska Energy Authority (AEA) has selected a site for the proposed development of a hydroelectric dam within Watana Canyon on the Upper Susitna River. The degree to which fish, in particular adult salmon, use this area as a migration corridor is currently uncertain. Reliable estimates of the number of fish that migrate through Watana Canyon will help describe the potential impacts on fishery resources of constructing and operating a hydropower project. Additionally, information regarding fish use of Watana Canyon as a migration corridor will inform discussions concerning fish passage facilities at the hydropower project. In its study plan determination (SPD), the FERC (Federal Energy Regulatory Commission) requested that AEA modify Study 9.7 to include an evaluation of the future feasibility of counting fish at or near the Watana Dam Site as follows (FERC 2013a, page B-20): “We recommend the study be modified to require AEA to include in the initial study report an evaluation, based on site-specific data obtained during the 2013 study season, of the feasibility of putting in a weir or sonar counting station at or near the dam site during the 2014 study season to provide an accurate count of any resident or anadromous fish that are successfully able to migrate upstream through Devils Canyon in the project area.” AEA followed up on FERC’s recommendation and determined that operation of a weir near the dam site was not feasible due to the physical impossibility of any structure handling the normal levels and range of discharge for the mainstem Susitna River. Further, it did not appear feasible to provide an accurate count of resident fish, or to know if a fish has migrated upstream through Devils Canyon (except for anadromous adult salmon). In 2013, the study team assessed the feasibility of placing a sonar counting station at or near the dam site (see Appendix G in AEA 2014c). Given several assumptions, it was likely feasible to count salmon-sized fish (50 cm [19.7 in] TL or greater) in Watana Canyon, in particular Chinook Salmon, and to quantify the accuracy of those counts using corroboration with the passage of radio-tagged Chinook Salmon (Appendix G in AEA 2014c). In 2014, the study team implemented the methods for achieving the objectives of sonar monitoring near the proposed Watana Dam site as described in the ISR. This appendix report describes the use of multi-beam imaging sonar for estimating the number of Chinook Salmon (fish 50 cm [19.7 in] TL or greater), as well as those measuring less than 50 cm (19.7 in) TL, that passed the proposed Watana Dam Site from July 6 to August 22, 2014. In addition, the study team collected bathymetry and water velocity profiles at the monitoring sites. 2. Study Goal and Objectives Following FERC’s recommendations, the primary goal of the study as modified by the feasibility assessment was to provide an accurate count of anadromous fish (specifically Chinook Salmon) near the proposed dam site (i.e., Watana Canyon). The specific objectives for this study were to: 1. Estimate the number of net upstream-moving Chinook Salmon that passed through the sonar beams; and STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 2 October 2015 2. Describe temporal (daily and hourly) and spatial (range of passage) patterns of Chinook Salmon observations. 3. STUDY AREA The study area was located in the Upper Susitna River near the location of the proposed Watana Dam Site. The section of river is characterized by a single channel with steep canyon walls, undisturbed forested areas, and gravel bars with occasional sand bars. 4. METHODS 4.1. Site Selection A reconnaissance flight was conducted on July 5, 2014, to assess the suitability of various locations for setting up sonar count stations. A location immediately downstream of the proposed Watana Dam Site at PRM 187.2 was determined to be the most suitable within the areas that were accessible. To find the optimal location for sonar sampling along the selected reach, an ARIS 1200 unit was used on July 6, 2014 to map the bathymetry along multiple transects from the right and left banks. Bottom-profiling allows for determination of optimal sonar alignment and aiming angles, and determination of the presence of depressions or troughs in the field-of-view (FOV) that would allow for fish to move past the sonar undetected (Maxwell and Smith 2007; Faulkner and Maxwell 2009). A single sonar location was established on each side of the river just below the proposed Watana Dam Site (Figure G-1). 4.2. Data Collection ARIS systems were used to monitor for Chinook Salmon escapement from each side of the river. Two different models of ARIS units were used: the ARIS 1200, which has operating frequencies of 0.7 and 1.2 megahertz (MHz), was installed on the left bank. The ARIS 1800, which has operating frequencies of 1.1 and 1.8 MHz, was installed on the right bank. Each ARIS system consisted of the sonar head, data transmission cable, switch box, Ethernet cable, laptop computer and an external hard drive. The sonar heads were deployed using aluminum H-mounts equipped with boat-trailer jacks that allowed for adjustment of sonar tilt angles. The mounts were typically positioned 5 to 10 ft from the edge of the wetted width (Figure G-2). The sonar heads were typically positioned between 0.2 and 1.5 ft off the bottom, aimed towards the opposite bank and tilted down to allow the sampling beams to spread across the substrate throughout the majority of the sampling windows. Sand bags filled with cobble and gravel were placed on the feet of the mounts to keep them secured to the substrate. The mounts, depth of sonar heads and tilt angles were adjusted periodically as river levels fluctuated throughout the study. Rock wall barriers were constructed to prevent fish from passing behind the mounts. Electronic components were housed in aluminum environmental boxes fastened to trees above mean high water level (Figure G-3). The systems were powered using banks of six, 6-V batteries that were recharged each day with Honda 2000 gasoline-powered generators. Data were acquired using ARISScope software (version 1.0 for the 1800 unit and version 2.0 for the 1200 unit, SoundMetrics Corp., Bellevue, WA). Data collection started at the left bank STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 3 October 2015 station at 4:51 P.M. on July 6 and at 12:12 P.M. on July 7 at the right bank station (see Table G-1 for sample site details). Data were collected continuously in consecutive 10-minute files until the study period ended on August 22 (with the exception of the period 10:40 A.M. on July 30 through 1:45 P.M. on August 7 when the left bank station was shut down). Data were ported directly to 1-terabyte external hard drives. Hard drives were changed daily and the data were backed up and archived to additional hard drives each day. The maximum sample ranges used were based on the extent to which substrate was visible in the FOVs. The gradually sloping bottom along the left bank allowed for substrate to be evident out to 37 m (121.4 ft) in range, whereas the bottom dropped off at 16 m (52.4 ft) in range along the right bank (Figure G-4). Seeing substrate throughout the FOV ensures that no depressions or troughs exist that would allow for fish to move past the ensonified area undetected. An aerial photograph of the sampling locations with depictions of the ensonified areas in plan -view is shown in Figure G-5. Operating frequencies and typical data collection parameters used for the two sonar stations are shown in Table G-2. There were sometimes slight adjustments to data collection parameters when mounts where repositioned to accommodate variable river levels (see Table G-6 for daily data collection parameters). 4.3. Data Processing Processing of ARIS data involved reviewing the data files using ARISFish software (version 1.0, SoundMetrics Corp.). Technicians in the field reviewed files by displaying the data in echogram form, which is a visual representation of the entire image file with time plotted along the horizontal axis and range plotted along the vertical axis. Fish targets that move across the FOV are shown as tracks. Reviewers scrolled through the echograms to locate tracks, and when tracks were observed the tracks were framed with the cursor to prompt the sonar imagery of the track to display (see Figure G-6 for example of this process). Sonar images of fish were enlarged and fish lengths were estimated using the software’s sizing tool. Fish targets were classified as resident fish or Chinook Salmon based on their estimated size. Chinook Salmon were defined as any fish targets that were 50 cm (19.7 in) or greater in estimated total length. For each Chinook Salmon detected, the following parameters were recorded: estimated total length, range at first and last detection, and direction of travel. Lengths of some fish could not be estimated when they passed immediately in front of the sonar units. If greater than or equal to 50 cm TL (19.7 in) of length could be measured for targe ts larger than the width of the FOV, then these fish were classified as Chinook with an unknown estimated length. Any fish targets measuring less than 50 cm (19.7 in) TL were not classified by species, and only their estimated total length was recorded. The accuracy of length measurements from the sonar data is approximately ± 10 percent based on known targets. Level-three quality control on the data review process was conducted in three steps by a senior scientist with sonar expertise: 1. All Chinook Salmon targets identified by field technicians were reviewed to ensure that each observation was accurate with respect to estimated size, first and last detected range, and direction of travel; STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 4 October 2015 2. All resident fish targets identified by field technicians and classified as being 40 to 49 cm (15.7–19.3 in) in estimated length were reviewed to ensure that none of these targets exceeded 49 cm (19.3 in) and thus should be classified as Chinook Salmon; and 3. Ten percent of the sonar data files from each day and from both monitoring stations were randomly selected and processed (as described above) to assess whether any Chinook Salmon observations were missed during the in-field review. 4.4. River Discharge Discharge data for the Upper Susitna River reported here were obtained from United States Geological Survey (USGS) gauge at Tsusena Creek. 4.5. Current Velocity and Bathymetry Profiles To support further assessment of the fish migration corridor at the sample reach, seven ADCP transects were measured at approximately 80-ft intervals along the reach (Figure G-5). The ADCP surveys were conducted on August 15, 2014. To supplement the velocity transects, bathymetric data were collected on August 16, 2014 using an Odom CV-100 echosounder and a TopCon GPS receiver along transects associated with sonar data collection (Transects 3 and 6). AEA (2014b) provides detailed methods associated with ADCP and bathymetry data collection. 5. RESULTS 5.1. River Discharge During sonar operations from July 6 to August 22, Susitna River flows at the Tsusena Creek gauge ranged from 14,200 to 35,300 cfs (Figure 4.1-3; 16,700–36,500 cfs at the Gold Creek gauge). Discharge in the Upper Susitna River generally decreased during the sonar sampling period after a peak of 35,300 cfs at the Tsusena Creek gauge on July 8 (36,500 cfs at the Gold Creek gauge). Periodic increases in discharge occurred in mid to late July. Throughout August, discharge remained below 19,000 cfs at the Tsusena Creek gauge (below 22,000 cfs at the Gold Creek gauge). 5.2. Sampling Effort With the exception of the period from July 30 through August 7 when the left bank station was demobilized, both stations operated continuously throughout the sample period (Figure G-7). 5.3. Sonar Coverage After initial setup of the sonar systems, the left bank station ensonified an estimated 41.5 percent of the wetted channel width and the right bank station ensonified an estimated 16.1 percent of the wetted channel width (Table G-3). With respect to overall scope, the systems covered 57.6 percent of the wetted channel widths, leaving 42.4 percent of the thalweg section of the river uncovered with sonar. Proportional coverage increased slightly as the mounts were periodically moved further out as water surface elevation decreased through the sampling period. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 5 October 2015 Cross-sectional coverage of the water column throughout the sampling ranges of the left and right bank sonar systems is shown in Figure G-8. The bottom profiles along the main axes of the sampling areas shown in Figure G-8 are based on geo-referenced data collected during the bathymetry surveys. The cross-sectional sonar coverage shown in Figure G-8 is conceptual given that the sonar heads were re-positioned and re-aimed throughout the study to accommodate changing river levels. The sonar systems sampled along the substrate throughout the entire range for the right bank sonar and 97 percent for the left bank sonar (see Figure G-4). Portions of the water column near the water surface at both locations were not sampled (top 2 ft) given the shape of the channel relative to the height of the sample volume. 5.4. Chinook Salmon Counts A total of 24 net upstream-migrating (26 upstream, 2 downstream) Chinook Salmon were counted at the sonar stations in 2014 (Table G-4). The first fish was observed on July 10 and last fish was observed on August 22. On a daily basis, Chinook counts ranged from 0 to 3, and counts greater than 0 generally coincided with decreasing or stabilized river discharge (Figure G- 9). Twenty-two of the 24 fish (92 percent) were observed with the right bank sonar station. On an hourly basis, the distribution of fish counts indicated no apparent diel passage patterns (Figure G-10). All fish detections were within 4 m (13.1 ft) from the sonar units with most occurring at 3 m (9.8 ft) in range (Figure G-11). Size estimates for upstream migrants ranged from 50 to 110 cm (19.7–43.3 in), with an average of 78 cm (30.7 in). In addition, 213 fish measuring 40–49 cm (15.7–19.3 in) TL, and 1,044 fish measuring less than 40 cm TL (15.7 in), were counted at the sonar stations (direction of movement for these fish was not recorded). These fish could not be identified to species using sonar. For fish which were less than 50 cm (19.7 in) TL, a percentage of these fish could potentially be small Chinook Salmon (as based on measurements at the Middle River fishwheels (minimum 27 cm [10.6 in] METF; see Figure A-29). However, the potential species as based on sampling from Study 9.5, and in order of likelihood in the observed size range, are Arctic grayling, burbot, round whitefish, and longnose sucker. 5.5. Water Velocity and Bathymetry Profiles A plot of serial water velocity profiles below the Watana dam site and along the reach of river where sonar sampling was conducted shows a general trend of lower velocities near the shorelines and higher velocities in the main channel (Figures G-12 to G-14). Comparing near- shore velocities between the left and right bank indicates that, with the exception of Transect 2, the River Right shorelines had lower velocities across a broader area than did the River Left shorelines. Transect 2 was unique among all transects in that the River Right edge terminated at a steep canyon wall that extended out onto a point (see Figure G-5). Velocities along River Right were typically less than 5 ft/s (1.5 m/s), whereas velocities along River Left were often greater than 5 ft/s. Velocities within the main channel often exceeded 10 ft/s (3 m/s) with transects 6 and 7 exhibiting the highest velocities. The river channel is characterized as having relatively steep banks that slope off to the maximum depth within 50 ft of ordinary high water level and a relatively flat in between (Figure G-15). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 6 October 2015 The velocity data were spatially integrated and represented as transect-specific discharge estimates (Table G-5). The discharge estimates provide measures of precision and accuracy on the velocity data. 6. DISCUSSION One hundred percent sonar coverage of the river width at PRM 182.1 was not possible in 2014 given the technical limitations of the sonar equipment and the physical bathymetry of the river. The maximum range at which 50 cm (19.7 in) TL targets can be detected with the ARIS 1200 unit is about 40 m (131 ft) and with the ARIS 1800 about 35 m (115 ft). Even with the most suitable bottom topography that would allow coverage along the substrate to the maximum ranges, coverage would still not achieve 100 percent. With the two ARIS units used in 2014, an estimated 58 percent of the river width was sampled. The sonar coverage was scaled to the extent of visible substrate. The un-sampled 42 percent consisted of primarily the middle of the river channel. The lack of complete sonar coverage creates uncertainty around the fish counts and raises two important questions – 1) Is there a more suitable location in the canyon that would provide higher proportional sonar coverage of the river width and thus less uncertainty around estimates of abundance?, and 2) How likely is it that some fish passed undetected in 2014 through the Watana Canyon in the main river channel beyond the maximum ranges sampled? Given the gradient and morphological features controlling the rivers width and depth in much of the Upper River, finding a location with a gradual and uniform substrate slope from river right to left that has a wetted width within the range needed for 100 percent sonar coverage is very unlikely. An aerial reconnaissance survey in 2014 did not identify any new potential sonar sites. All potential locations with a wetted width closer to the range needed for 100 percent sonar coverage had unsuitable bottom profiles and depths for the multi-beam sonar technology to provide effective coverage. Many locations are inaccessible by helicopter, and others provide no option for a site installation, even by boat, due to the steep rock walls present along many sections of the river. There may be suitable locations below Tsusena Creek, but establishing count stations below a major tributary creates uncertainty regarding whether the fish detected at the site will spawn above the Watana Dam site or up in the tributary. Presently, the count stations established in 2014 are the most suitable locations for monitoring fish passage above the dam site with sonar. To address the question of whether fish may have passed the count stations undetected beyond the sampling ranges used in 2014, it is informative to examine water velocity and bathymetry profile across the width of the river to understand the physical environment and potential fish migration corridors. Bathymetry and velocity surveys were conducted at seven transects in the river reach in which sonar data were collected during the 2014 sample period. Profile data from these surveys provide important information collected concurrently with the sonar data to help determine potential migration corridors and aid in assessing effectiveness of the sonar sampling near the dam site. The 2014 water velocity profiles (Figures G-12 to G-14) indicate higher water velocities with increasing distance from shore, and generally, a greater extent of lower water velocities along the right bank as compared with the left bank. Given the availability of low water velocity areas along the banks (especially along the right bank) as compared with the high velocities observed STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 7 October 2015 throughout the main channel, it is likely the primary migration corridors in this section of river are along the banks. The velocity is too high for a majority of the channel to be conducive to fish passage. Further, there does not appear to be any unique bathymetry which would provide a corridor for passage except for relatively close to the banks (Figure G-15). It is important to point out that the velocity data were collected when discharge of the river was 14,900 cfs. This level of discharge reflects river conditions throughout much of the latter half of the study, but does not reflect conditions earlier in the study period when discharge was considerably higher. Nonetheless, it likely that the general pattern of high velocities in the main channel and lower velocities along the banks would persist at higher flow levels. Overall, the precision of the velocity data was estimated to be within 1–2 percent, but the accuracy of the data showed a slight negative bias relative to USGS gauge data (D. Brailey, Brailey Hydrologic Consultants, pers. comm). Data are being evaluated across the past three years relative to USGS data to quantify accuracy of the velocity data; the current estimate is about a 1 to 2 percent negative bias. With respect to the likelihood of fish passing through the canyon in the high-velocity areas beyond the reach of the sonar count stations, it is instructive to review what is known about swimming speeds and capabilities of migrant adult salmon. Previous research on Chinook Salmon swimming capabilities (Watts 1974; Bell 1986) suggest a sustained swimming speed (normal function for long periods of time without fatigue) of 0.0–3.4 ft/s (0.0 to 1.0 m/s), and a prolonged swimming speed (sustainable for 15 s to 200 s) of 3.1–10.8 ft/s (1.0 to 3.3 m/s). A swimming speed of greater than 10.8 ft/s (3.3 m/s) is considered sustainable for 15 s or less. At river discharges of 27,600 cfs and greater, based on the presumed swimming capabilities of Chinook Salmon and the measured water velocities shown in Figures G-13 and G-14, fish passage along the river right and river left banks would not be expected outside of 65 ft (20 m) and 33 ft (10 m) from shore, respectively. The ADF&G does not have any data that suggest Chinook Salmon negotiate flows greater than 9.8 ft/s (3 m/s) at any of their sonar sampling locations throughout the state, but they do not rule out the possibility (Debby Burwen and Bruce McIntosh, ADF&G, pers. comm., July 7, 2014). The fish count data collected in 2014 support the general notion that migrating salmon use low velocity areas as migration corridors when available. The highly skewed distribution of counts towards the right bank as compared with the left bank follows the 2014 velocity profile patterns that show greater proportions of low velocity areas along the right bank as compared with the left bank. Additionally, the distribution of counts as a function of distance from the sonar units indicates that fish passage through the canyon was shore-oriented with all detections occurring within 4 m (13.1 ft) of the units. If some fish did migrate up through the main channel undetected, it is reasonable to assume that the distribution of counts would have included detections further out in range from the sonar units. The sonar data, together with velocity profile data and known swimming capabilities of salmon, suggest there is a low probability that some fish passed through the canyon undetected due to incomplete sonar coverage. To aid in assessing efficiency of the sonar systems, two telemetry receiver stations were deployed about 300 feet upstream from the sonar units on the right bank. One tagged Chinook Salmon was detected by the receivers at 11:19 P.M. on July 31. Unfortunately the timing of this detection coincided with the period in which the left bank count station had been demobilized. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 8 October 2015 There were no sonar detections of fish measuring 50 cm (19.7 in) TL or greater from the right bank station around the time of the detected tagged fish. Presumably that fish passed along the left bank, or less likely up the main channel. Due to the limited number of tagged fish that migrated up as far as Watana Canyon (just that one fish) telemetry data could not be used to assess efficiency of the sonar systems. An important issue that contributed uncertainty regarding whether some targets were of sufficient size to classify them as Chinook Salmon involved fish that passed extremely close to the sonar units. Some fish either moved through the blanking range (within 0.7 m [2.3 ft] of the units), or through the most narrow part of the sample volume in the near -field. It was not possible to measure these fish. Of 1,366 total observations, 100 (7.3 percent) could not be measured for length. As a result, it is technically possible that some Chinook Salmon may have passed by undetected. Given that 2.5 percent of the observations where a length was determined were classified as Chinook Salmon, it is reasonable to estimate that 3 of 100 observations without a length were Chinook Salmon. 7. CONCLUSIONS The results of the sonar sampling in 2014 led to the following conclusions:  A net total of 24 upstream-migrant Chinook Salmon were counted, and this should be considered a minimum estimate of Chinook Salmon abundance in the Watana Canyon;  Daily passage through the canyon was low, ranging from 0 to 3 fish per day;  There were no apparent diel passage patterns;  The distribution of passage was highly skewed towards the right bank; and  Distributions of fish detections as a function of distance from the sonar units indicate that passage was shore-oriented. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 9 October 2015 Table G-1. Location details for Watana Canyon sonar sites near PRM 187.1 in 2014. Table G-2. Operating frequencies and data collection parameters used for the ARIS monitoring stations at Watana Canyon in 2014. Left Bank Right Bank Operating Frequency (MHz) 0.7 1.1 Sample Window Start (m) 0.7 (2.3 ft) 0.7 (2.3 ft) Sample Window End (m) 39.2 (128.6 ft) 16.6 (54.5 ft) Sample Rate (frames per second) 5.4 6.8 Sonar Heading (degrees) 329 140 Sonar Tilt Angle (degrees) -9.6 -15.1 Sonar Roll Angle (degrees) 1.7 0.5 Table G-3. Percent coverage for each sonar station and combined based on wetted channel width, wetted edge to sonar, and ensonified range at sample sites near PRM 187.1 in Watana Canyon. Install Date ARIS Unit Sonar Location Latitude Longitude Wetted Channel Width (m) Wetted Edge to Sonar (m) 06-Jul 1200 River Left 62.82214 -148.5381 96 (315 ft)1.5 (4.9 ft) 07-Jul 1800 River Right 62.82307 -148.5370 106.1 (348 ft)3 (9.8 ft) Combined Sonar Location Wetted Channel Width (m) Wetted Edge to Sonar (m) Ensonified Range (m)Coverage % River Left 96 (315 ft)1.5 (4.9 ft)39.2 (128.6 ft)41.5 River Right 106.1 (348 ft)3 (9.8 ft)16.6 (54.5 ft)16.1 Combined 57.6 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 10 October 2015 Table G-4. Sample effort, CPUE, and net upstream count of fish measuring 50 cm or greater at two ARIS units located at PRM 187.1 in the Upper River, 2014. Mean daily discharge of the Susitna River at Tsusena Creek is also shown. Table G-4. Continued. Date Upstream Down- stream Net Upstream Sample Effort (h) CPUE (fish/h)Upstream Down- stream Net Upstream Sample Effort (h) CPUE (fish/h) 06-Jul 0 0 0 7.1 0.00 23,648 07-Jul 0 0 0 24.0 0.00 0 0 0 11.8 0.00 31,521 08-Jul 0 0 0 24.0 0.00 0 0 0 24.0 0.00 35,331 09-Jul 0 0 0 24.0 0.00 0 0 0 24.0 0.00 29,431 10-Jul 0 0 0 24.0 0.00 1 0 1 24.0 0.04 28,232 11-Jul 0 0 0 24.0 0.00 0 0 0 23.7 0.00 27,668 12-Jul 0 0 0 24.0 0.00 0 1 -1 24.0 -0.04 30,000 13-Jul 0 0 0 24.0 0.00 0 0 0 24.0 0.00 31,527 14-Jul 0 0 0 24.0 0.00 0 0 0 24.0 0.00 31,069 15-Jul 0 0 0 24.0 0.00 0 0 0 24.0 0.00 25,300 16-Jul 0 0 0 24.0 0.00 2 0 2 24.0 0.08 21,900 17-Jul 0 0 0 24.0 0.00 1 0 1 24.0 0.04 19,900 18-Jul 0 0 0 24.0 0.00 0 0 0 24.0 0.00 18,700 19-Jul 0 0 0 24.0 0.00 1 1 0 24.0 0.00 18,500 20-Jul 0 0 0 24.0 0.00 1 0 1 24.0 0.04 21,100 21-Jul 0 0 0 24.0 0.00 0 0 0 24.0 0.00 23,400 22-Jul 0 0 0 24.0 0.00 1 0 1 24.0 0.04 20,400 23-Jul 0 0 0 24.0 0.00 1 0 1 24.0 0.04 17,800 24-Jul 0 0 0 24.0 0.00 1 0 1 24.0 0.04 17,800 25-Jul 1 0 1 24.0 0.04 1 0 1 24.0 0.04 17,600 26-Jul 0 0 0 24.0 0.00 0 0 0 24.0 0.00 20,000 27-Jul 0 0 0 24.0 0.00 1 0 1 24.0 0.04 18,600 28-Jul 0 0 0 24.0 0.00 0 0 0 24.0 0.00 16,500 29-Jul 0 0 0 24.0 0.00 0 0 0 24.0 0.00 16,100 30-Jul 0 0 0 10.7 0.00 0 0 0 24.0 0.00 15,500 Mean Discharge (cfs) River RightRiver Left Fish Count Fish Count STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 11 October 2015 Date Upstream Down- stream Net Upstream Sample Effort (h) CPUE (fish/h)Upstream Down- stream Net Upstream Sample Effort (h) CPUE (fish/h) 31-Jul 2 0 2 24.0 0.08 15,600 01-Aug 3 0 3 24.0 0.13 15,700 02-Aug 2 0 2 24.0 0.08 15,900 03-Aug 1 0 1 24.0 0.04 16,200 04-Aug 0 0 0 24.0 0.00 16,200 05-Aug 1 0 1 24.0 0.04 16,600 06-Aug 1 0 1 24.0 0.04 17,300 07-Aug 0 0 0 10.3 0.00 0 0 0 23.9 0.00 16,200 08-Aug 0 0 0 24.0 0.00 1 0 1 24.0 0.04 15,600 09-Aug 0 0 0 24.0 0.00 0 0 0 24.0 0.00 15,700 10-Aug 0 0 0 24.0 0.00 0 0 0 24.0 0.00 14,800 11-Aug 0 0 0 23.7 0.00 0 0 0 24.0 0.00 14,200 12-Aug 0 0 0 24.0 0.00 0 0 0 24.0 0.00 14,700 13-Aug 0 0 0 24.0 0.00 0 0 0 24.0 0.00 14,800 14-Aug 0 0 0 24.0 0.00 0 0 0 24.0 0.00 14,500 15-Aug 0 0 0 24.0 0.00 1 0 1 24.0 0.04 14,700 16-Aug 1 0 1 24.0 0.04 0 0 0 24.0 0.00 16,400 17-Aug 0 0 0 24.0 0.00 0 0 0 24.0 0.00 17,300 18-Aug 0 0 0 24.0 0.00 0 0 0 24.0 0.00 18,000 19-Aug 0 0 0 23.8 0.00 0 0 0 24.0 0.00 17,700 20-Aug 0 0 0 24.0 0.00 0 0 0 24.0 0.00 16,200 21-Aug 0 0 0 24.0 0.00 0 0 0 24.0 0.00 15,400 22-Aug 0 0 0 10.1 0.00 1 0 1 12.0 0.08 14,700 Total 2 0 2 891.5 24 2 22 1067.2 River left sonar not operational Mean Discharge (cfs) River RightRiver Left Fish Count Fish Count STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 12 October 2015 Table G-5. Discharge estimates based on spatial integration of velocity data for ADCP surveys conducted in the Watana Canyon in August, 2014. Transect Start Edge Start Time Duration Width (ft) Area (ft2) Mean Speed (ft/s) Left Q (cfs) Right Q (cfs) Total Q (cfs) Percent Measured T7 Right Bank 14:08 0:02:25 344.1 2176 6.9 -0.8 -1.7 14,993 74.7 T7 Left Bank 14:19 0:04:06 329.5 2069 7.2 -4.7 2.6 14,980 74.9 T6 Right Bank 14:39 0:03:09 324.7 2265 6.6 8.7 0.8 15,058 73.6 T6 Left Bank 14:59 0:04:07 320.2 2200 6.8 8.3 0.6 14,988 73.9 T5 Right Bank 15:19 0:03:27 299.9 2132 6.7 3.8 0.9 14,601 74.3 T5 Left Bank 15:23 0:03:18 306.0 2179 6.7 5.4 0.9 14,913 74.5 T4 Right Bank 16:16 0:03:27 327.8 2347 6.3 0.4 1.0 15,267 74.8 T4 Left Bank 16:31 0:03:06 327.4 2338 6.4 2.4 -3.1 15,125 75.3 T3 Left Bank 16:42 0:02:39 305.4 2418 6.0 5.8 -2.9 14,698 75.8 T3 Right Bank 16:46 0:03:12 309.2 2465 6.0 3.2 24.3 15,102 75.7 T2 Right Bank 17:00 0:02:40 273.4 2401 6.1 3.9 23.5 15,030 76.4 T2 Left Bank 17:03 0:02:43 265.8 2496 5.9 3.1 25.9 14,895 76.2 T1 Left Bank 17:11 0:02:36 282.7 2414 6.2 6.2 1.2 14,890 76.3 T1 Right Bank 17:20 0:02:30 276.7 2359 6.1 4.7 1.5 14,346 74.5 Mean 0:03:06 306.6 2304 6.4 3.6 5.4 14,920 75.1 Std Dev 0:00:32 23.3 129 0.4 3.4 10.2 227 0.9 COV 0.076 0.056 0.063 0.95 1.9 0.015 0.011 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 13 October 2015 Table G-6. Daily data collection parameters at the Watana Canyon sonar sites, 2014. Date Time (hh:mm) Sample Location Range Start (m) Range End (m) Sonar Heading (deg.) Sonar Tilt Angle (deg.) Crew 6 Jul 16:51 River Left 2.9 39.2 329 -9.6 PJohnson, KShippen, SBurril 7 Jul 12:11 River Right 0.7 16.6 50 -17.0 KShippen, PJohnson 14:52 River Left 0.7 39.1 330 -9.5 8 Jul 11:53 River Right 0.7 16.5 50 -17.0 KShippen, PJohnson 11:38 River Left 0.7 39.1 327 -10.0 9 Jul 08:40 River Right 0.7 16.5 49 -17.1 KShippen, SBurril 08:12 River Left 0.7 39.1 327 -9.7 10 Jul 08:53 River Right 0.7 16.5 50 -17.1 KShippen, SBurril 08:36 River Left 0.7 39.1 327 -9.9 11 Jul 09:34 River Right 0.7 16.5 47 -15.5 KShippen, SBurril 08:59 River Left 0.7 38.9 327 -9.8 12 Jul 08:22 River Right 0.7 16.5 47 -15.4 KShippen, JBures 08:42 River Left 0.7 38.9 326 -9.8 13 Jul 11:45 River Right 0.7 16.5 47 -16.5 KShippen, JBures 11:26 River Left 0.7 38.9 327 -9.8 14 Jul 08:28 River Right 0.7 16.5 48 -15.3 KShippen, JBures 09:09 River Left 0.7 38.9 324 -13.2 15 Jul 12:32 River Right 0.7 16.5 48 -15.4 KShippen, JBures 08:35 River Left 0.7 38.9 325 -13.2 16 Jul 09:23 River Right 0.7 16.5 50 -16.3 KShippen, JBures 08:44 River Left 0.7 38.9 324 -13.2 17 Jul 09:41 River Right 0.7 16.5 48 -15.2 KShippen, JBures 10:23 River Left 0.7 38.9 322 -12.6 18 Jul 11:55 River Right 0.7 16.5 47 -15.1 JBures, LGasek 12:26 River Left 0.7 39.1 318 -11.6 19 Jul 8:48 River Right 0.7 16.5 47 -15.2 JBures, LGasek 09:02 River Left 0.7 39.1 318 -11.6 20 Jul 8:48 River Right 0.7 16.5 47 -15.1 JBures, LGasek 09:10 River Left 0.7 39.0 318 -11.6 21 Jul 09:00 River Right 0.7 16.5 47 -15.6 JBures, LGasek 08:39 River Left 0.7 38.9 317 -11.5 22 Jul 12:03 River Right 0.7 16.5 56 -15.7 JBures, LGasek 12:22 River Left 0.7 39.0 318 -11.4 23 Jul 08:46 River Right 0.7 16.6 55 -14.9 JBures, LGasek 09:05 River Left 0.7 39.1 317 -11.5 24 Jul 09:53 River Right 0.7 16.5 54 -14.2 JBures, LGasek 09:38 River Left 0.7 39.0 317 -11.5 25 Jul 08:50 River Right 0.7 16.5 53 -14.2 JBures, LGasek 09:00 River Left 0.7 39.0 317 -11.5 26 Jul 08:51 River Right 0.7 16.5 53 -14.2 JBures, LGasek 09:07 River Left 0.7 38.8 317 -11.5 27 Jul 08:40 River Right 0.7 16.5 53 -14.2 JBures, LGasek 08:51 River Left 0.7 38.9 317 -11.5 28 Jul 10:15 River Right 0.7 16.5 53 -13.9 JBures, LGasek 10:55 River Left 0.7 39.0 334 -9.1 29 Jul 11:00 River Right 0.7 16.5 53 -14.1 JBures, LGasek 11:21 River Left 0.7 39.0 336 -9.2 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 14 October 2015 Table G-6. Continued. Date Time (hh:mm) Sample Location Range Start (m) Range End (m) Sonar Heading (deg.) Sonar Tilt Angle (deg.) Crew 30 Jul 13:20 River Right 0.7 16.5 54 -14.0 JBures, LGasek 10:43 River Left 0.7 39.0 336 -9.1 31 Jul 09:28 River Right 0.7 16.6 54 -14.3 JBures, LGasek 1 Aug 09:24 River Right 0.7 16.6 54 -14.2 JBures, LGasek 2 Aug 08:46 River Right 0.7 16.5 54 -14.2 JBerry, LFerreira 3 Aug 09:36 River Right 0.7 16.5 54 -14.1 JBerry, LFerreira 4 Aug 09:56 River Right 0.7 16.5 54 -14.2 JBerry, LFerreira 5 Aug 09:37 River Right 0.7 16.5 54 -14.2 JBerry, LFerreira 6 Aug 11:33 River Right 0.7 16.5 53 -14.1 JBerry, LFerreira 7 Aug 09:56 River Right 0.7 16.5 54 -14.2 JBerry, LFerreira 13:47 River Left 0.7 39.5 327 -8.4 8 Aug 08:23 River Right 0.7 16.5 54 -14.2 JBerry, LFerreira 08:53 River Left 0.7 39.5 329 -8.3 9 Aug 08:40 River Right 0.7 16.5 54 -14.1 JBerry, LFerreira 08:29 River Left 0.7 39.5 329 -8.4 10 Aug 08:27 River Right 0.7 16.5 54 -14.2 JBerry, LFerreira 08:16 River Left 0.7 39.5 330 -8.3 11 Aug 08:13 River Right 0.7 16.6 54 -14.1 JBerry, LFerreira 08:30 River Left 0.7 39.5 330 -10.0 12 Aug 08:31 River Right 0.7 16.6 52 -13.0 JBerry, LFerreira 08:12 River Left 0.7 39.5 330 -10.0 13 Aug 08:26 River Right 0.7 16.6 52 -13.0 JBerry, LFerreira 08:14 River Left 0.7 39.5 329 -10.0 14 Aug 08:26 River Right 0.7 16.5 52 -13.0 JBerry, LFerreira 08:15 River Left 0.7 39.5 330 -10.0 15 Aug 08:19 River Right 0.7 16.5 52 -13.0 JBerry, NCollin 08:37 River Left 0.7 39.6 330 -10.0 16 Aug 11:07 River Right 0.7 16.5 52 -13.0 JBerry, NCollin 10:55 River Left 0.7 39.5 330 -10.2 17 Aug 08:21 River Right 0.7 16.4 51 -13.1 JBerry, NCollin 08:39 River Left 0.7 39.6 330 -10.1 18 Aug 08:30 River Right 0.7 16.4 51 -13.1 JBerry, NCollin 09:12 River Left 0.7 39.5 330 -10.1 19 Aug 08:10 River Right 0.7 16.5 46 -10.2 JBerry, NCollin 08:35 River Left 0.7 38.8 331 -8.4 20 Aug 09:04 River Right 0.7 16.5 50 -11.8 JBerry, NCollin 08:46 River Left 0.7 39.0 332 -8.4 21 Aug 08:03 River Right 0.7 16.5 56 -14.1 JBerry, NCollin 08:20 River Left 0.7 39.0 332 -8.4 22 Aug 08:39 River Right 0.7 16.5 55 -14.0 JBerry, NCollin 09:00 River Left 0.7 39.0 332 -8.4 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 15 October 2015 Figure G-1. Photograph of the Susitna River immediately downstream of the proposed Watana Dam Site (PRM 187.1) showing the location of the river left and river right sonar sites and the wetted channel width. Figure G-2. Photographs showing the ARIS mounts deployed at the left bank (left) and right bank (right) monitoring stations. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 16 October 2015 Figure G-3. Photographs showing the environmental boxes used to house the ARIS systems electronic components, and power sources (battery banks are inside action packers) for the left bank (left) and right bank (right) monitoring stations. Figure G-4. Still images from ARIS data showing the cobble substrate (light-colored structure) along the left bank (left) and right bank (right) fields-of-view. Substrate is visible to 38 and 16 m in range from the left and right bank FOVs, respectively. Note range markers are shown in 2-m increments on left and 1-m increments on right. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 17 October 2015 Figure G-5. Ortho image showing the ensonified wetted width coverage of each ARIS unit near the Watana Dam Site, 2014. Ensonified coverage is scaled to match the width of the river. River flow is from right to left. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 18 October 2015 Figure G-6. Screen shots of Right Bank ARIS data showing echograms (left) and still sonar imagery (right) for a resident fish (top) and Chinook Salmon (bottom). Echogram tracks are shown inside the black rectangles and the fish targets in the imagery data are shown inside the white circles. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 19 October 2015 Date (m/d) Figure G-7. Daily sampling effort at two ARIS sonar units located at PRM 187.1 in the Upper River, 2014. 0 4 8 12 16 20 24 7/67/87/107/127/147/167/187/207/227/247/267/287/308/18/38/58/78/98/118/138/158/178/198/218/23Left Bank (PRM 187.08) 0 4 8 12 16 20 24 7/67/87/107/127/147/167/187/207/227/247/267/287/308/18/38/58/78/98/118/138/158/178/198/218/23Right Bank (PRM 187.12)Sampling Effort (hr) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 20 October 2015 Figure G-8. Bathymetry profiles derived from ADCP data for transects aligned with ARIS sampling locations (PRM 187.1). Typical cross-sectional coverage of ARIS sample areas are depicted with the overlaid triangles. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 21 October 2015 Figure G-9. Net upstream count of fish measuring 50 cm or greater at two ARIS sonar units located at PRM 187.1 in the Upper River, 2014. Discharge of the Susitna River at Tsusena Creek is also shown. Hour of the Day Figure G-10. Diel migration of fish measuring 50 cm or greater counted at two ARIS sonar units located at PRM 187.1 in the Upper River. Only upstream-moving fish are shown. 10,000 14,000 18,000 22,000 26,000 30,000 34,000 38,000 0 1 2 3 4 5 7/67/87/107/127/147/167/187/207/227/247/267/287/308/18/38/58/78/98/118/138/158/178/198/218/23Discharge (cfs)Net Upstream Fish CountDate (m/d) River Left River Right Discharge 0 1 2 3 4 5 01234567891011121314151617181920212223River Left 0 1 2 3 4 01234567891011121314151617181920212223River Right Number of Fish Counted STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 22 October 2015 Figure G-11. Percent of fish measuring 50 cm or greater counted at two ARIS sonar units located at PRM 187.1 in the Upper River as a function of distance from the sonar units, 2014. The mid-range distance was calculated as the average of where a fish was first and last detected in the FOV. Only upstream-moving fish are shown. Figure G-12. Series of velocity profiles collected along transects using an ADCP at Watana Canyon (PRM 187.1) in 2014. Transects are arranged from upstream (top) to downstream (bottom) to allow for best presentation of the transect-to-transect channel morphology. Range of sonar stations is shown for the River Left site at Transect 3 and River Right site at Transect 6. 0 10 20 30 40 50 60 70 0 1 2 3 4 5 6 7 8 9 10 Distance from Sonar Units (m) River Left River Right Percent of Fish STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 23 October 2015 Figure G-13. Individual velocity profile for Transect 3 in Watana Canyon (PRM 187.1) collected with an ADCP in 2014. This profile corresponds to the location of the sonar station on River Left (ensonified zone illustrated). Figure G-14. Individual velocity profile for Transect 6 in Watana Canyon (PRM 187.1) collected with an ADCP in 2014. This profile corresponds to the location of the sonar station on River Right (ensonified zone illustrated). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix G – Page 24 October 2015 Figure G-15. Bathymetry in Watana Canyon (PRM 187.1) based on seven serial ADCP transects, 2014. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 October 2015 Appendix H: Indian River Escapement Estimate for chinook salmon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix H – Page 1 October 2015 1. INDIAN RIVER ESCAPEMENT ESTIMATE Two components are required to estimate abundance using AUC methods: 1) an estimate of the number of fish-days derived from an area under the escapement curve; and 2) an estimate of the residence time, or the length of time that fish are alive in the survey area. For this study, the escapement curve was derived from Chinook Salmon counts made during aerial spawner surveys, and residence time was estimated from detections of radio-tagged Chinook Salmon made during aerial telemetry surveys in the Indian River. Aerial spawner surveys were conducted in the Indian River every third day from early July to late August to count the number of live Chinook Salmon present in the river. Aerial spawner counts were stratified into three river sections. Section 1 extended from the clear -water plume at the confluence with the Susitna River up to Bridge 1 in the lower Indian River; Section 2 ranged from Bridge 1 to the power-line crossing; and Section 3 ranged from the power-line crossing to the Forks. Due to the resolution available from radio telemetry, the study team could not definitively assign radio-tagged Chinook Salmon detected in proximity to the confluence (below Bridge 1) to either Section 1 or the turbid waters of the mainstem Susitna River. To estimate residence time (and mark rates), it was important that the aerial spawner counts and radio-tag detections correspond to the same survey areas, thus all subsequent AUC calculations were germane to the area upstream of Bridge 1 (i.e., only data from river sections 2 and 3 were used). For each river section, crews recorded their observed counts and an estimate of observer efficiency. As part of the AUC calculations described below, the estimates of observer efficiency were used to ‘adjust’ the observed counts. Observer efficiency was estimated for each river section because there were differences in the physical characteristics of each (e.g., gradient, substrate, flow dynamics, vegetation cover); and these differences can influence an observer’s ability to count fish. Environmental conditions used to estimate observer efficiency included water clarity, sun glare, and vegetation cover, all of which were ranked on a scale of 0 to 4 (0 is poor, 4 is optimal). Survey crews also took weather conditions and professional judgment into account when estimating observer efficiency for each river section. Aerial telemetry surveys were also conducted in the Indian River every third day from early July to late August, on the same days as the aerial spawner surveys. Aerial telemetry surveys were used to count the number of radio-tagged Chinook Salmon in each of the river sections, and to record whether the radio-tagged fish were alive or dead. As described by English et al. (1992), the number of live fish present in the study area on the ith day (pi) was estimated by: 𝑛�ℎ=∑𝑒𝑛𝐿�=1 ∙𝑁�∙𝑛𝑟�ℎ�−1 ∙𝑛𝑒�ℎ�−1 (1) where: L is the number of spatial strata (h) within each stream; foih is the fish observed during surveys in stratum h on the ith sampling day; nh is the number of sampling units in stratum h; nsih is the number of units surveyed in stratum h on the ith sampling day; and oeih is the observer efficiency. The area under the escapement curve (auc) was estimated by: STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix H – Page 2 October 2015 𝑎𝑟𝑐=0.5 ∙∑(𝑟�ℎ−𝑟�ℎ)∙(𝑛�ℎ+𝑛�ℎ−1 )𝑛 �ℎ=2 (2) where: ti is the number of days measured from the first day fish enter the survey area to the ith sampling day; and n-2 is the number of surveys when fish were seen. An escapement estimate (N) was then calculated as: 𝑁= 𝑎𝑟𝑐𝑟𝑟⁄ (3) Residence time (rt) of Chinook Salmon in the Indian River was estimated using radio-tagged fish that were released in the Lower and Middle rivers. All radio tags were equipped with a mortality sensor that changed the signal pattern to an “inactive” mode for the remainder of the season once the tag became stationary for 24 hours. Residence times for individual fish were calculated as the cumulative length of time spent alive in the study area. Aerial telemetry surveys were not flown daily, so the dates that some fish died were approximated. Median residence times were used in AUC calculations. Escapement estimates derived using AUC methods are dependent on the input values for observer efficiency and residence time. Despite using consistent aerial surveying methods with an experienced crew, and having access to telemetry data from dozens of radio-tagged fish in the Indian River, the estimates of these parameters were potentially biased to some degree in 2014. Validation was compromised by the Indian River weir being rendered inoperable prior to the onset of the Chinook Salmon run, so there were no weir counts available to ‘ground-truth’ the aerial spawner surveys. As such, the study team conducted a sensitivity analysis to determine the potential impact of reducing estimates of observer efficiency on the escapement estimate. 2. MARK RATE The mark rate, or the proportion of the Chinook Salmon run that was tagged, was estimated for the Middle River fishwheels by dividing the total number of large, radio-tagged Chinook Salmon released in the Middle River that were detected above Bridge 1 by the estimated escapement of fish above Bridge 1. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix H – Page 3 October 2015 Table H-1. Number of Chinook Salmon counted during aerial spawner surveys in the Indian River, and the number of radio-tagged large Chinook Salmon detected, by tag site, 2014. These data are preliminary, and additional aerial spawner surveys are planned for August (these data will be added to the table later). Survey Date River Section a Observer Efficiency Observed Adjusted Middle River Lower River Middle River Lower River Comment 07-Jul 1 60%36 60 x x Good conditions 2 70%91 130 17 2 Most fish holding in pools 3 80%0 0 1 0 pool count estimated Total 127 190 13.8%1.5% 10-Jul 1 50%82 164 x x Fair conditions 2 60%184 307 20 2 dark, light rain 3 70%29 41 3 1 Most fish holding in pools Total 295 512 6.6%0.9%pool count estimated 14-Jul 1 60%123 205 x x Good conditions 2 70%233 333 48 3 Some fish holding in pools 3 80%72 90 10 2 pool count estimated Total 428 628 13.7%1.2% 17-Jul 1 60%110 183 x x Good conditions 2 70%389 556 67 5 Less fish in pools 3 80%101 126 15 4 Spawning activity Total 600 865 12.0%1.3% 19-Jul 1 40%61 153 x x Poor conditions 2 50%330 660 68 3 Bad weather 3 60%56 93 20 5 dark and rainy Total 447 906 11.7%1.1% 22-Jul 1 60%160 267 x x Excellent conditions 2 70%490 700 77 5 Fish evenly distributed 3 80%148 185 28 3 on spawning grounds Total 798 1,152 11.9%0.9% 26-Jul 1 50%70 140 x x Fair conditions 2 60%327 545 85 6 Turbidity in lower reaches 3 80%108 135 30 5 following high water event Total 505 820 16.9%1.6% 29-Jul 1 60%67 112 x x Excellent conditions 2 70%379 541 83 4 Fish redistributed 3 80%160 200 26 5 following high water event Total 606 853 14.7%1.2% 01 Aug b 1 60%47 78 x x Good conditions 2 70%351 501 82 6 Corresponding aerial 3 80%146 183 22 3 telemetry survey was Total 544 762 15.2%1.3%conducted on July 31 Live Tags Detected c Mark Ratec (by Tag Site)(by Tag Site)Aerial Count STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix H – Page 4 October 2015 Table H-1. Continued. Survey Date River Section a Observer Efficiency Observed Adjusted Middle River Lower River Middle River Lower River Comment 03-Aug 1 60%59 98 x x Good conditions 2 70%323 461 47 4 3 80%96 120 16 5 Total 478 680 10.8%1.5% 06-Aug 1 60%34 57 x x Good conditions 2 70%214 306 38 4 3 80%58 73 11 4 Total 306 435 13.0%2.1% 09-Aug 1 60%18 30 x x Good conditions 2 70%127 181 32 3 3 80%24 30 7 3 Total 169 241 18.4%2.8% 12-Aug 1 60%6 10 x x Good conditions 2 70%55 79 22 2 3 80%14 18 3 2 Total 75 106 26.0%4.2% 15-Aug 1 60%5 8 x x Good conditions 2 70%16 23 13 2 3 80%4 5 3 2 Total 25 36 57.4%14.4% 18-Aug 1 60%0 0 x x Good conditions 2 70%2 3 4 3 3 80%1 1 3 3 Total 3 4 170.4%146.1% 19-Aug 1 60%0 0 Good conditions 2 70%2 3 3 80%1 1 Total 3 4 a Section 1 = clearwater plume to Bridge 1; Section 2 = Bridge 1 to Powerline; Section 3 = Powerline to Forks. b The aerial telemetry survey corresponding to the August 1 aerial spawner survey was conducted on July 31. c Counts of live tags (and mark rate calculations) were based on data collected in sections 2 and 3 and excluded section 1. No aerial telemetry survey was flown on 8/19 Live Tags Detected c Mark Ratec (by Tag Site)(by Tag Site)Aerial Count STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix H – Page 5 October 2015 Table H-2. Summary of AUC abundance estimate, mark rate at the Middle River tag site, and the expected number of fish passed the Watana Dam sonar site, 2014. Figure H-1. Number of Chinook Salmon counted during aerial spawner surveys above Bridge 1 in the Indian River, 2014. Observed counts (dashed red line) for each survey were expanded by reach-specific estimates of observer efficiency to derive ‘adjusted’ counts (solid blue line). The zero count on June 25 was estimated; no Chinook Salmon were observed passing the Indian River weir through June 25 (prior to it being washed out by a high-water event). The zero count on August 20 was estimated (surveyors indicated that the three remaining live Chinook Salmon observed on August 19 were moribund). Area under the escapement curve (fish days)20,280 Median residence time (days)15.6 Estimated abundance of large Chinook salmon above Bridge 1 1,297 Number of Middle River tags above Bridge 1 171 Mark rate at Middle River fishwheels (%)13.2 One of every 'X' fish was tagged in Middle River, where 'X' =7.6 Expected number of fish passed Watana given that one tag passed =7.6 Expected number tags passed Watana given sonar count of 24 fish =3.2 0 150 300 450 600 750 900 6/256/276/297/17/37/57/77/97/117/137/157/177/197/217/237/257/277/297/318/28/48/68/88/108/128/148/168/188/20Number of FishDate (m/d) Observed Adjusted No fish counted at weir as of 6/25 First aerial spawner survey on 7/7 Assumed no live fish left by 8/20 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix H – Page 6 October 2015 Figure H-2. Relative frequency of residence times (days) for radio-tagged Chinook Salmon above Bridge 1 in the Indian River, 2014. 0% 5% 10% 15% 20% 25%0-55-1010-1515-2020-2525-3030-3535-4040-4545-5050-5555-6060-65Relative FrequencyResidence Time (d) Chinook Salmon above Rail Bridge in Indian River (days to death or departure) Median = 15.6 days (n = 184) STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 October 2015 Appendix I: Estimate the system-wide Chinook Salmon escapement, Coho Slamon escapement above the Yentna River, and the distribution of those fish among tributaries of the susitna river STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 1 June 2015 1. INTRODUCTION This appendix presents the data analyses pertinent to the estimation of Chinook and Coho salmon abundance in the Susitna River upstream of the confluence with the Yentna River, and Chinook Salmon abundance in the Yentna River drainage. 1.1. Study Objective The Chinook and Coho salmon abundance and distribution estimates reported herein are a component of the following study objective. From Section 2, Objective 8: 8) Estimate the system-wide Chinook Salmon escapement to the entire Susitna River, the Coho Salmon escapement to the Susitna River above the confluence with the Yentna River, and the distribution of Chinook, Coho, and Pink salmon among tributaries of the Susitna River (upstream of Yentna River confluence) in 2013 and 2014. 2. STUDY AREA The section of the Susitna River upstream of the confluence with the Yentna River is hereafter referred to as the “mainstem Susitna River” and encompasses the “Lower,” “Middle,” and “Upper” sections of the Susitna River as defined in Section 3. For Yentna River Chinook Salmon, the abundance and spawning distribution estimates are germane to the Chinook Salmon population passing the marking sites on the lower Yentna River at river mile 6. 3. METHODS See Section 4.8. 4. RESULTS 4.1. Estimated Abundance of Chinook Salmon 4.1.1. Mainstem Susitna River Above the Yentna River Confluence A total of 2,048 Chinook Salmon of all sizes were captured in drift gillnets and two fishwheels at the lower mainstem Susitna River tagging site (PRM 34) from May 22 to August 19, 2014. Radio tags were deployed on 659 Chinook Salmon measuring 50 cm (19.7 in) METF or greater. Based on observations made at fixed-station receiver sites and using aerial radio-telemetry, 494 of these radio-tagged fish moved upstream and remained one mile upstream of the east bank fishwheel and therefore met criteria to be in the mark-recapture experiment. The remaining 165 (659–494) radio-tagged Chinook Salmon either migrated to the Yentna River drainage or the Susitna River below PRM 34, failed to migrate due to handling stress, or were above the tagging site and not detected (unlikely). It was also possible that some tags were regurgitated below the point at which the tagged fish were categorized as being part of the mark-recapture experiment (one mile upstream of the east bank fishwheel). Second event sampling was conducted at weirs on the Deshka River and at Montana Creek. A total of 16,335 Chinook Salmon were counted through the Deshka River weir from May 19 to September 2, 2014, with 13,908 of these fish STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 2 October 2015 estimated to be 50 cm (19.7 in) METF or greater. At the Montana Creek weir, 1,217 Chinook Salmon were counted from June 23 to September 11, of which 1,212 fish were estimated to be 50 cm (19.7 in) METF or greater. Second event sampling was also conducted at a site on the Chulitna River using telemetry and an ARIS sonar system. However, the sonar echogram quality, counts, and length determinations suggest the data to be unreliable and they were not used. The estimated numbers of Chinook Salmon measuring 50 cm (19.7 in) METF or greater that passed the Deshka River and Montana Creek weirs comprised the second event of the mark - recapture experiment. Based on observations made at fixed-station receivers and using aerial radio-telemetry, 125 radio-tagged Chinook Salmon passed the Deshka River weir and were assumed to have spawned above the weir. Similarly, 15 radio-tagged Chinook Salmon passed the Montana Creek weir and were assumed to have spawned above the weir. Flood events at the Deshka weir on June 28–29 and at the Montana Creek weir on June 26–30 prevented accurate counting. Recaptures associated with these periods were removed from the data (one tag was removed for each of the Deshka River and Montana Creek datasets). The remaining 138 recaptures (125+15-2) were used in the mark-recapture experiment. Tests for size-biased sampling in the marking and recapture events were conducted using the KS two-sample test (Cleary et al. 2013). The tests for the second sampling event, which compared the length distributions of marked and recaptured fish, provided significant evidence of size biased sampling (P = 0.016; Figure I-1). The test for size-biased sampling during the first event using data from fish passing the Deshka River weir provided significant evidence of size biased sampling (P < 0.001; Figure I-2). A similar test for size-biased sampling during the first event using data from fish passing the Montana Creek weir also provided evidence of size biased sampling (P = 0.026, Figure I-3), although the length of the maximum D-value, and hence the suggested stratification point, was considerably smaller. Given that the sample sizes at the Montana weir were substantially lower than those at the Deshka River weir results of the first-event KS test using data from the Deshka River weir were preferred. Montana Creek weir counts (of fish measuring 50 cm [19.7 in] or greater) and recaptures were approximately 9 percent and 11 percent of those at the Deshka River weir. Based on these test results, the data were stratified into two size strata: 50.0–78.5 cm (19.7–30.9 in) METF, and 78.5 cm (30.9 in) METF or greater. Re-running the KS tests for size selectivity within each size category failed to detect any selectivity at α = 0.05. Moreover, for each of the size categories, one of the two KS tests described above was not significant at α = 0.2. Tests for consistency of the Chapman (1951) model for estimation of abundance with respect to temporal and/or spatial variation in probability of capture were conducted within the each of the 2 size strata. These tests were first described by Seber (1982; p. 438–439) and subsequently described as the ‘Equal Proportions Test” (EP) and the “Complete Mixing Test” (CM) by Arnason et al. (1996). A passing EP or CM test means the Chapman model is valid. The EP tests examine the consistency of marked fractions in the second event sample over spatial and temporal strata. The EP tests were only approximate, because the number of fish inspected for STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 3 October 2015 marks during second event sampling within each stratum was not known, but was estimated using size composition data collected at each weir. Before the EP tests were conducted an adjustment to the timing of the recaptures at the weirs was made; this adjustment affected the EP tests on temporal strata. Analysis of the timing of weir counts and recaptures at the Deshka weir strongly suggested a tag-induced lag for the recaptured fish. The median run timing of the untagged fish was significantly earlier than that of the recaptured fish (Mann-Whitney test; W = 764, 482; P < 0.001); the median difference was estimated at 4 days (Figure I-4). Tag-induced delay in migration has been previously observed in Chinook Salmon (Bendock and Alexandersdottir 1993; Bernard et al. 1999). The latter authors found a lag of approximately 4–5 days before radio-tagged Chinook Salmon in the Kenai River, Alaska, resumed upstream migration. Recoveries at the Montana Creek weir were too sparse to show an effect. The run timing of the recaptures was adjusted at the Deshka and Montana weirs by moving them 4 days earlier (note that the flood adjustment described earlier was performed on the lag-adjusted data). For the 50.0–78.5 cm (19.7–30.9 in) METF stratum, the EP test passed on the spatial (Test 3 in Table I-1; P = 0.921) and temporal scales (Tests 4 and 5 in Table I-1; P = 0.09 and 0.189 for Deshka and Montana, respectively). The CM test passed on a temporal scale (Test 2 in Table I- 1; P = 0.311), but not on a spatial scale (Test 1 in Table I-1; P < 0.001). These results indicate that the Chapman model is appropriate for estimating abundance for the 50.0–78.5 cm (19.7– 30.9 in) METF stratum. For the 78.5 cm (30.9 in) METF or greater stratum, the EP test passed on the temporal scale (Tests 4 and 5 in Table I-2; P = 0.70 and 1 for Deshka and Montana, respectively); the EP test did not pass on the spatial scale (Test 3 in Table I-2; P = 0.001). The CM test passed on the spatial and temporal scale (Tests 1 and 2 in Table I-2; P = 0.187 and P = 0.148, respectively). It is noted that the contingency tables used in Table I-2 are sparse, and the sample sizes border the conditions at which the chi-square tests are not strictly valid. As given, however, these results indicate that the Chapman model is appropriate for estimating abundance for the 50.0–78.5 cm (19.7–30.9 in) METF stratum; moreover, no stratified design would be possible with the sparse sample sizes in this category. Abundance for each stratum was estimated using the Chapman (1951) model. The distribution of mark-recapture estimates is recognized to be frequently asymmetric (e.g., Zwane and van der Heijden 2003), thus the utility of analytically-estimated standard errors in calculating confidence intervals is limited. Also, the analytical formula of variance of the Chapman estimate of abundance assumes the number of fish examined in the second event for tags is a constant. In this experiment length samples were used to estimate the number of fish measuring 50 cm (19.7 in) METF or greater inspected at the weirs for tags. Finally, estimating the number of fish examined in each stratum creates dependency among the stratum-specific abundance estimates because higher estimates of small fish necessarily means smaller estimates of larger fish and vice versa. For these reasons, variances and confidence intervals were estimated using a parametric bootstrap (e.g., Buckland and Garthwaite 1991). Within each size stratum, the probability that a marked fish was recaptured was modeled as a hypergeometric process with observed numbers of fish marked, estimated number inspected in STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 4 October 2015 the second event and estimated abundance as model parameters. For each of the two weir sites, the numbers of fish inspected for marks was modeled as a multinomial process with total observed passage at the weir and the empirical size distribution data collected at each weir used to define model parameters. A 100,000 parametric bootstrap samples were generated. Variance of the abundance estimate of fish of each size class was calculated as the sample variance of the 100,000 bootstrap estimates of each estimate. Variance of the abundance estimate of all fish measuring 50 cm (19.7 in) METF or greater was calculated as the sample variance of the 100,000 bootstrap estimates of the sum of the abundance of the two size classes (Table I-3). Confidence intervals reported in Table I-4 are percentiles of the parametric bootstrap distributions of estimated parameters. The abundance of Chinook Salmon measuring 50 cm (19.7 in) METF or greater spawning in the Susitna River above the mainstem tagging site in 2014 is estimated to be 68,225 (SE = 10,615). 4.1.2. Yentna River A total of 3,071 Chinook Salmon were captured in drift gillnets and two fishwheels at the Yentna River tagging site (RM 6) from May 22 to August 25, 2014. Dart tags were deployed on 1,281 Chinook Salmon measuring 50 cm (19.7 in) METF or greater. Radio tags were also deployed on 296 of the dart-tagged fish. These radio-tagged fish were used to a) estimate the drop-out rate of the dart-tagged fish due to handling mortality and migration to the mainstem Susitna drainage, and b) estimate spawning distribution in the Yentna drainage. Based on observations made at fixed tracking stations and using aerial radio telemetry, 219 of the radio-tagged fish moved upstream and remained at least one mile upstream of the east bank fishwheel for at least 48 hours and therefore met criteria to be used in the mark recapture study. The remaining 77 (296-219) radio-tagged Chinook Salmon either migrated to the Susitna River drainage or the Susitna River below the Yentna-mainstem Susitna confluence, failed to migrate due to handling stress, or migrated above the tagging site and not detected (unlikely). The number of deployed dart tags was deprecated based on the proportion of radio tags failing to enter the mark-recapture experiment. Of the 1,281 dart tags deployed, it is estimated that 963 tags entered the mark- recapture experiment. With respect to the spawning distribution study, 5 radio-tagged fish were harvested and 7 made it to the recapture wheels but then died, leaving only 207 (219-12) radio- tagged fish for inclusion in the distribution study. Second event sampling was conducted using two fishwheels and a mid-river gillnet at RM 18. A total of 2,308 Chinook Salmon were caught in the second event sampling from May 24 to August 22, 2014, with 1,371 of these fish measuring 50 cm (19.7 in) METF or greater. Second event sampling recovered 59 dart tags, 25 on the west fishwheel, 31 on the east fishwheel, and 3 in the gillnet. Ten of the 59 recovered dart tagged fish were also radio tagged. Tests for size-biased sampling in the marking and recapture events were conducted using the KS two-sample test (Appendix A1 in Cleary et al. 2013). The test for the second sampling event, which compared the length distributions of marked and recaptured fish, provided no evidence of size biased sampling (P = 0.65, D = 0.088; Figure I-5). The tests for size-biased sampling during the first event using data from fish passing the fishwheels and gillnet at RM 6 provided no significant evidence of size biased sampling STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 5 October 2015 (P = 0.12, D = 0.196 for the east fishwheel, P = 0.67, D = 0.131 for the west fishwheel and P = 0.97, D = 0.053 for pooled fishwheel and gillnet data; Figures I-6, I-7, and I-8). No test was performed for the second event gillnet-only data as there were too few recaptures (3). Based on these test results, the data were pooled into one size class (all fish ≥ 50 cm [19.7 in] METF). Tests for consistency of the Chapman (1951) model for estimation of abundance with respect to temporal and/or spatial variation in probability of capture were conducted. These tests were first described by Seber (1982, p. 438–439) and subsequently described as the ‘Equal Proportions Test” (EP) and the “Complete Mixing Test” (CM) by Arnason et al. (1996). A passing EP or CM test means the Chapman model is valid. The EP tests examine the consistency of marked fractions in the second event sample over spatial and temporal strata. Before the EP tests were conducted an adjustment to the timing of the recaptures at the second event capture site was made; this adjustment affected the EP tests on temporal strata. Analysis of the timing of fishwheel catches (≥ 50 cm [19.7 in] METF) and recaptures at the recapture wheels strongly suggested a tag-induced lag for the recaptured fish. The median run timing of the untagged fish was significantly earlier than that of the recaptured fish (Mann-Whitney test, W = 46,870, P = 0.019); the median difference was estimated at 3 days (Figure I-9). Tag-induced delay in migration has been previously observed in Chinook Salmon (Bendock and Alexandersdottir 1993; Bernard et al. 1999). The latter authors found a lag of approximately 4–5 days before radio-tagged Chinook Salmon in the Kenai River, Alaska, resumed upstream migration. Moreover, a tag-induced lag of 4 days was also detected for radio-tagged fish in the mainstem Susitna mark recapture component of this study in 2014. The run timing of the recaptures in the current study was adjusted at the second event fishwheels by moving the recapture date 3 days earlier. The EP test passed on the spatial (Test 3 in Table I-5; P = 0.58) and temporal scales (Tests 4-6 in Table I-5; P = 0.83, P = 0.55 and P = 0.44 for the east fishwheel, west fishwheel and pooled gear, respectively). The CM test passed on the spatial scale (Test 1 in Table I-5; P = 0.68) and on the temporal scale (Test 2 in Table I-5; P = 0.35). These results indicate that the Chapman model is appropriate for estimating abundance for the Chinook Salmon measuring 50 cm (19.7 in) METF or greater on the Yentna River. The distribution of mark recapture estimates is recognized to be frequently asymmetric (e.g. Zwane and van der Heijden 2003), thus the utility of analytically-estimated standard errors in calculating confidence intervals is limited. Also, the analytical formula of variance of the Chapman estimate of abundance assumes the number of fish marked in the first event is a constant. In this experiment, we used radio tag migration data to estimate the proportion of marked fish that migrated into the mark-recapture experiment. For these reasons, variances and confidence intervals were estimated using a parametric bootstrap (e.g., Buckland and Garthwaite 1991). We note that we found variation in the downstream migration pattern of radio-tagged Chinook Salmon among (two) different crews. Pooled over gear types, fish radio-tagged by one crew (A) experienced a drop-out rate of about 33 percent, while the rate for the other crew was (B) was about 12 percent. Consistently, the recapture rate of dart-tagged fish for crew A was about 2.8 percent, while for crew B it was 6.2 percent. The influence on tagged fish by crew B was not therefore limited to the act of radio-tagging, and appears related to general handling of fish. It is STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 6 October 2015 also noted that timing of radio-tagging or type of gear appears not to explain the differences in drop-out rate between crews. To accommodate these differences in the abundance analysis, we applied crew-specific drop-out rates to marked fish. For the simulation, the probability that a marked fish was recaptured was modeled as a hypergeometric process with estimated number of fish marked, number inspected in the second event, and estimated abundance as model parameters. For each of the two crews, the numbers of fish exhibiting downstream migration was modeled as a binomial process with number of radio tags deployed and the estimated drop-out rate used to define model parameters. A sample of 50,000 parametric bootstrap realizations was generated. Variance of the abundance estimate was calculated as the sample variance of the 50,000 bootstrap estimates of each estimate. The 95 percent confidence interval for the abundance estimate was calculated as the 2.5 and 97.5 percentiles of the parametric bootstrap distributions of the estimated abundance. The abundance of Chinook Salmon measuring 50 cm (19.7 in) METF or greater spawning in the Yentna River above the lower tagging site in 2014 is estimated to be 22,267 (SE = 2,871; CV = 13 percent; Table I-6). The 95 percent parametric bootstrap confidence interval is 17,466 to 28,701. 4.2. Estimated Abundance of Coho Salmon A total of 1,513 Coho Salmon were captured in two fishwheels at the lower mainstem Susitna River tagging site (PRM 34) from June 28 to August 26, 2014. Radio tags were deployed on 640 Coho Salmon measuring 40 cm (15.7 in) METF or greater. Based on observations made at fixed tracking stations and using aerial radio-telemetry, 582 of these radio-tagged fish moved upstream and remained one mile upstream of the east bank fishwheel and therefore met criteria to be included in the mark- recapture experiment. The remaining 58 (640-582) radio-tagged Coho Salmon either migrated to the Yentna River drainage or the Susitna River below PRM 34, failed to migrate due to handling stress, or were above the tagging site and not detected (unlikely). It is also possible that some tags were regurgitated below the point at which the tagged fish were categorized as being part of the mark-recapture experiment (one mile upstream of the east bank fishwheel). Second event sampling was conducted at weirs on the Deshka River and at Montana Creek. A total of 11,578 Coho Salmon were counted through the Deshka River weir from July 4 to September 2, 2014, with all of them estimated to be 40 cm (15.7 in) METF or greater. At the Montana Creek weir 934 Coho Salmon were counted from August 3 to September 21; all were estimated to be 40 cm (15.7 in) METF or greater. The estimated number of Coho Salmon measuring 40 cm (15.7 in) METF or greater that passed the Deshka River and Montana Creek weirs comprised the second event of the mark-recapture experiment. Based on observations made at fixed tracking stations and using aerial radio-telemetry, 68 radio- tagged Coho Salmon passed the Deshka River weir and were assumed to have spawned above the weir. Similarly, 4 radio-tagged Coho Salmon passed the Montana Creek weir and were assumed to have spawned above the weir. A total of 72 recaptures (68 + 4) were used in the mark-recapture experiment. Tests for size-biased sampling in the marking and recapture events were conducted using the KS two-sample test (Appendix A1 in Cleary et al. 2013). The tests for the second sampling event, STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 7 October 2015 which compared the length distributions of marked and recaptured fish, provided significant evidence of size biased sampling (P < 0.001; Figure I-10). The test for size-biased sampling during the first event using data from fish passing the Deshka River weir provided significant evidence of size biased sampling (P ≤ 0.001, Figure I-11). No test for size-biased sampling during the first event was conducted using data from the Montana Creek weir because only four fish were recaptured at that site. Based on the test results of the above KS tests, the data were stratified into two size strata: 40–55 cm (15.7–21.7 in) METF, and 55 cm (21.7 in) METF or greater. Rerunning the KS tests for size selectivity within each size category indicated that no further size stratification was needed. Tests for consistency of the Chapman (1951) model for estimation of abundance with respect to temporal and/or spatial variation in probability of capture were conducted within the each of the 2 size strata. These tests were first described by Seber (1982; hypotheses H2 and H4 on p. 438– 439) and were subsequently described as the “Complete Mixing Test” (CM; H2) and the ‘Equal Proportions Test” (EP; H4) by Arnason et al. (1996). A passing EP or CM test indicates the Chapman model is valid. The EP tests examine the consistency of marked fractions in the second event sample over spatial and temporal strata. The EP tests were only approximate, because the number of fish inspected for marks during second event sampling within each stratum was not known, but was estimated using size composition data collected at each weir. No adjustment to the timing of the recaptures at the weirs was made; we found no evidence of a difference in the timing at the Deshka weir of tagged and untagged fish. For the 40–55 cm (15.7–21.7 in) METF stratum, the EP test passed on the spatial (Test 3 in Table I-7; P = 0.382) and temporal scales (Test 4 in Table I-7; P = 0.217 for the Deshka recovery site). There were too few recoveries at Montana to conduct the test for this site. The CM test passed on a temporal scale (Test 2 in Table I-7; P = 0.225), but not on a spatial scale (Test 1 in Table I-7; P ≤ 0.001). These results indicate that the Chapman model is appropriate for estimating abundance for the 40–55 cm (15.7–21.7 in) METF stratum. For the 55 cm (21.7 in) METF or greater stratum, the EP test passed on the spatial scale (Test 3 in Table I-8; P ~ 1.0) and temporal scales (Test 4 in Table I-8; P = 0.13 for the Deshka recovery site). There were too few recoveries at Montana to conduct the test for this site. The CM test passed on the temporal scale (Test 2 in Table I-8; P = 0.113), but not the spatial scale (Test 1 in Table I-8; P = 0.006). These results indicate that the Chapman model is appropriate for estimating abundance for the 55 cm (21.7 in) METF or greater stratum. Abundance for each stratum was estimated using the Chapman (1951) model. The distribution of mark recapture estimates is recognized to be frequently asymmetric (e.g., Zwane and van der Heijden 2003), thus the utility of analytically-estimated standard errors in calculating confidence intervals is limited. Also, the analytical formula of variance of the Chapman estimate of abundance assumes the number of fish examined in the second event for tags is a constant. In this experiment length samples were used to estimate the number of fish within each size stratum that were inspected at the weirs for tags. Finally, estimating the number of fish examined in each stratum creates dependency among the stratum-specific abundance estimates because higher STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 8 October 2015 estimates of small fish necessarily means smaller estimates of larger fish and vice versa. For these reasons, variances and confidence intervals were estimated using a parametric bootstrap (e.g., Buckland and Garthwaite 1991). Within each size stratum, the probability that a marked fish was recaptured was modeled as a hypergeometric process with observed numbers of fish marked, estimated number inspected in the second event and estimated abundance as model parameters. For each of the two weir sites, the numbers of fish inspected for marks was modeled as a multinomial process with total observed passage at the weir and the empirical size distribution data collected at each weir used to define model parameters. A sample of 100,000 parametric bootstrap realizations was generated. Variance of the abundance estimate of fish of each size class was calculated as the sample variance of the 100,000 bootstrap estimates of each estimate. Variance of the abundance estimate of all fish measuring 40 cm (15.7 in) METF or greater was calculated as the sample variance of the 100,000 bootstrap estimates of the sum of the abundance of the two size classes (Table I-9). Confidence intervals reported in Table I-4 are percentiles of the parametric bootstrap distributions of estimated parameters. The abundance of Coho Salmon measuring 40 cm (15.7 in) METF or greater spawning in the Susitna River above the mainstem tagging site in 2014 is estimated to be 84,879 (SE = 9,550; Table I-10). 4.3. Estimated Distribution of Spawning Salmon 4.3.1. Chinook Salmon 4.3.1.1. Mainstem Susitna River Above the Yentna River Confluence Results from the mark–recapture experiment indicate that radio tags were not deployed in Chinook Salmon proportional to the size distribution of fish in the population. To estimate abundance of spawning salmon in different tributaries within the mainstem Susitna River drainage, the number of spawners among tributaries was first estimated within each size stratum. Numbers of fish by tributary within a size stratum was then calculated as the product of the proportion by tributary within size stratum and estimated abundance for that size stratum (Table I-3). Numbers of fish were then summed over size strata for each tributary. 4.3.1.2. Yentna River No size selectivity was found for the marked fish in the mark-recapture analysis described above. We also found no significant difference between the size distributions of the radio-tagged fish and the marked fish used in the mark-recapture analysis (D = 0.084, P = 0.1); we assume therefore there to be no size selectivity among the radio-tagged fish. Recoveries of radio tags in the second sampling event were too sparse to conduct the CM tests for radio tags. It is noted, however, that no significant CM tests were found in the mark-recapture analysis involving (primarily) darts tags, and given that radio tags were applied systematically to all Chinook Salmon measuring 50 cm (19.7 in) METF or greater, we assume that a representative sample of fish measuring 50 cm (19.7 in) METF or greater were radio-tagged in the first sampling event. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 9 October 2015 To estimate abundance of spawning salmon in different tributaries within the Yentna River drainage, the proportion of spawners among tributaries was first estimated. Number of fish by tributary was then calculated as the product of the proportion by tributary and estimated abundance (Table I-6). 4.3.2. Coho Salmon Results from the mark–recapture experiment indicate that radio tags were not deployed in Coho Salmon proportional to the size distribution of fish in the population. To estimate abundance of spawning salmon in different tributaries within the mainstem Susitna River drainage, the number of spawners among tributaries was first estimated within each size stratum. Numbers of fish by tributary within a size stratum was then calculated as the product of the proportion by tributary within size stratum and estimated abundance for that size stratum (Table I-9). Numbers of fish were then summed over size strata for each tributary (Table I-10). 5. DISCUSSION An estimate of Chinook Salmon abundance for the Susitna River upstream of the Yentna River confluence was generated after size-stratification. It is unfortunate that the diagnostic KS tests suggested a stratification point at the higher end of the length scale (78.5 cm METF [30.9 in]), because it meant the estimate of abundance for fish of the large size category was based on relatively few recaptures (12 fish). It is noted that the pooled estimate of all Chinook Salmon measuring 50 cm (19.7 in) METF or greater is 53,246 (22 percent smaller), demonstrating the influence of size-stratification. The objective to estimate the distribution of the Chinook Salmon escapements among the tributaries of the Susitna River above the Yentna River was successful. With the estimate of the Yentna River Chinook Salmon abundance for 2014 complete, the objective to estimate the system-wide Chinook Salmon escapement in 2014 has now been met. The marking and capture techniques and choice of locations provided sufficient sample sizes to achieve a reasonably precise abundance estimate, with a coefficient of variation of 13 percent. The marking and capture techniques and locations also provided conditions that allowed for consistent spatial and temporal marking of fish, without being size selective (except for one instance in the RM 18 east fishwheel). Not having to stratify the data helped to minimize the variance of the abundance estimate. Defining fish that entered the experiment was occasionally difficult as there was no stationary telemetry site at the RM 18 recapture site. It had to be assumed that all fish passing the lower Yentna stationary telemetry site (approximately RM 9) and staying upstream of that site for at least 48 hours, continued upstream to the recapture site at RM 18. This is an important assumption, as it affects the dropout rate, which affects the number of tags out, and thus the entire abundance estimate. While most radio-tagged fish passing the lower Yentna stationary telemetry site continued upstream to later be detected in aerial telemetry surveys and confirmed as in the experiment, some did not. An estimate of 84,879 Coho Salmon migrating into the Susitna River upstream of the Yentna River confluence was generated after size-stratification. Without stratification a biased estimate of approximately 100,000 Coho Salmon is calculated (17 percent larger). It is apparent that smaller fish are marked at a higher rate than larger fish at the lower Susi tna River fishwheels. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 10 October 2015 The objective to estimate the distribution of the Coho Salmon escapements among the tributaries of the Susitna River above the Yentna River was successful. A weakness in the overall design of the 2014 project is that we only had two recapture sites, one of which (Montana) was associated with relatively few fish, and therefore recaptures (4). The small number of recaptures at Montana compromises the EP spatial test, leaving us to largely assume the fish passing the Deshka river weir were tagged at the same rate as the other stocks in the drainage. 6. CONCLUSIONS AND RECOMMENDATIONS The 2014 mark-recapture experiment for Chinook Salmon in the mainstem Susitna River appears to have been successful. It is recommended, however that the differential tagging rates of fish 50–58 cm (19.7–22.8 in) METF be maintained in future experiments of this kind, but additionally, that more effort also be directed into tagging larger fish. It is noted that a sizeable portion of the estimated abundance of Chinook Salmon was based on a relatively small portion of the recaptured fish. Larger fish are typically tagged via gillnets and concern over the mortality rate of this method of sampling has hindered tagging larger fish. Adjustments to gillnet sampling may be required such that more large fish (METF ≥ 58 cm [22.8 in]) can be tagged without undue mortality. An additional recapture event is recommended on Clear Creek, replacing the failed attempts on the Chulitna River. Clear Creek is shallower and more accessible than the Chulitna River site, allowing more attention to the sonar operation, and has a larger Chinook Salmon escapement, which should result in more recaptured fish. Tethered fish experiments, crucial to calibration of sonar-derived length data, should also be easier at this site. The 2014 mark-recapture experiment for Chinook Salmon in the Yentna River appears to have been successful with the current design. It is recommended, however, that the reasons for the substantially differential drop-out rates among crews be investigated before further similar work is done, and efforts taken to standardize the sampling protocol between crews and minimize handling time. Adding a stationary telemetry site at the RM 18 recapture site would clearly identify when tagged fish become available for recapture and increase the accuracy of the dropout rate estimate. The 2014 mark-recapture experiment for Coho Salmon in the mainstem Susitna River appears to have been successful. It is, however, recommended that additional recapture sites be considered in the future that allow assessment of the tagging rates of other stocks. These sites could be additional weirs or upriver fishwheels. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 11 October 2015 Table I-1. Diagnostic tests for mark-recapture data for mainstem Susitna River Chinook Salmon measuring 50.0–78.5 cm METF, 2014. Testa Parameter 1) CM-Spatialb Gear Gillnet Fishwheel 1 west Fishwheel 2 east Marks 42 148 191 Recaptured 6 66 55 Not recaptured 36 82 137 2) CM-Temporalc Julian day 142-156 157-162 163-181 Marks 164 120 97 Recaptured 48 42 37 Not recaptured 116 79 60 3) EP-Spatiald Weir site Deshka R Montana Cr Inspectede 10,138 952 Marked 116 10 Unmarked 10,022 942 4) EP-Temporalf Deshka weir Julian day 138-159 160-162 163-167 168-246 Inspectede 1941 2974 2852 2371 Marked 30 25 29 32 Unmarked 1911 2949 2823 2339 5) EP-Temporalg Montana weir Julian day 174-191 192-203 204-254 Inspectede 339 328 285 Marked 3 6 1 Unmarked 336 322 284 a CM = “Complete Mixing Test” and EP = “Equal Proportions Test” (see text; Arnason et al. 1996). b χ2 = 17.22, P < 0.001. c χ2 = 2.332, P = 0.311. d χ2 =0.01, P = 0.921. e Number of fish inspected for marks is estimated. f χ2 = 6.48, P = 0.09. g χ2 = 3.33, P = 0.189. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 12 October 2015 Table I-2. Diagnostic tests for mark-recapture data for mainstem Susitna River Chinook Salmon measuring 78.5 cm METF or greater, 2014. Testa Parameter 1) CM-Spatialb Gear Gillnet Fishwheel 1 west Fishwheel 2 east Marks 45 24 43 Recaptured 4 5 3 Not recaptured 41 19 40 2) CM-Temporalc Julian day 142-156 157-162 Marks 45 67 Recaptured 2 10 Not recaptured 43 57 3) EP-Spatiald Weir site Deshka R Montana Cr Inspectede 3770 260 Marked 8 4 Unmarked 3762 256 4) EP-Temporalf Deshka weir Julian day 138-162 162-246 Inspectede 1906 1864 Marked 3 5 Unmarked 1903 1859 5) EP-Temporalg Montana weir Julian day 174-198 199-254 Inspectede 93 167 Marked 1 3 Unmarked 92 164 a CM = “Complete Mixing Test” and EP = “Equal Proportions Test” (see text; Arnason et al. 1996). b χ2 = 3.35, P = 0.187 c χ2 = 2.09, P = 0.148 d χ2 = 10.28, P = 0.001 e Number of fish inspected for marks is estimated. f χ2 = 0.148, P = 0.70 g χ2 = 0, P = 1 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 13 October 2015 Table I-3. Estimated abundance, number of radio tags deployed, and relative weights (number of spawners per tag) used to estimate abundance within size stratum for Chinook Salmon spawning upstream from the lower mainstem tagging site in the Susitna River, 2014. Size Strata Estimated Abundance Estimated SE Radio Tags Deployed Relative Weight spawners/tag 50.0 - 78.5 cm METF 33,184 2,783 381 86.6 ≥ 78.5 cm METF 35,041 10,645 112 312.9 > 50 cm METF 68,225 10,615 Table I-4. Chinook Salmon measuring 50 cm METF or greater spawning distributions, based on weighted abundance (Table I-3), in the mainstem Susitna River above the Lower River tagging site, 2014. Estimated Abundance Intervals Location SE 95% lower 95% upper Susitna River above the mainstem tagging site 68,225 10,615 53,473 94,240 PRM 34–102.4 mainstem Susitna River a 2,098 682 1,064 3,717 Deshka River 14,024 816 12,451 15,667 Eastside Susitna River 15,073 3,398 10873 23,939 Talkeetna River 14,024 3,713 9,622 23,657 PRM 102.4–153.4 mainstem Susitna River b 6,609 2,365 3,781 12,700 Chulitna River 16,397 3,961 11,653 26,708 a PRM 34 upstream to the Chulitna River Confluence. b Chulitna River Confluence to Devils Canyon. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 14 October 2015 Table I-5. Diagnostic tests for mark-recapture data for Yentna River Chinook Salmon >50 cm METF, 2014a. Test Parameter 1) CM-Spatialb Gear North Fishwheel South Fishwheel Gillnet Marked 311 396 256 Recaptured 16 26 17 Not recaptured 295 370 239 2) CM-Temporalc Julian day 146-154 155-159 160-176 Marked 335 298 330 Recaptured 25 14 20 Not recaptured 310 284 310 3) EP-Spatiald Gear East Fishwheel West Fishwheel Gillnet Inspected 725 540 120 Marked 31 25 3 Unmarked 694 515 117 4) EP-Temporale East 2nd event fishwheel Julian day 144-157 158-163 164-234 Inspected 322 181 222 Markedh 15 8 8 Unmarked 307 173 214 5) EP-Temporalf West 2nd event fishwheel Julian day 144-157 158-163 164-234 Inspected 172 213 155 Markedh 8 12 5 Unmarked 164 201 150 6) EP-Temporalg Both 2nd event fishwheels Julian day 144-157 158-163 164-234 plus gillnet Inspected 513 419 453 Markedh 23 21 15 Unmarked 490 398 438 a CM=”Complete Mixing Test” and EP=”Equal Proportions Test” (see text; Arnason et al. 1996) b χ2 = 0.77, P =0.68; Marks are estimated. c χ2 = 2.1, P =0.35; Marks are estimated. d χ2 =1.1, P = 0.58 e χ2 =0.37, P = 0.83 r χ2 = 1.18, P = 0.55 g χ2 = 1.64, P = 0.44 h Julian days for marked fish adjusted for estimated 3 day lag; see text for details. STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 15 October 2015 Table I-6. Chinook Salmon spawning distributions in the Yentna River above the RM 6 tagging site, 2014. Estimated Abundance Intervals Location SE 95% lower 95% upper Yentna River above tagging site 22,267 2,871 17,466 28,701 Lake Creek drainage 5,163 986 3,496 7,334 Kahiltna River drainage 4,195 855 2,746 6,082 Talachulitna River drainage 1,721 482 892 2,783 Skwentna River drainage, other than the Talachulinta River drainage 4,303 868 2,824 6,213 Remaining Yentna River drainage, other than the areas above 6,885 1,214 4,805 9,557 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 16 October 2015 Table I-7. Diagnostic tests for mark-recapture data for mainstem Susitna River Coho Salmon 40-55 cm METF, 2014a. Test 1) CM-Spatialb Gear Fishwheel 1 west Fishwheel 2 east Marks 191 203 Recaptured 34 4 Not recaptured 157 199 2) CM-Temporalc Julian day 188-207 208-212 213-232 Marks 155 128 111 Recaptured 13 17 8 Not recaptured 142 111 103 3) EP-Spatiald Weir site Deshka R Montana Cr Inspectede 3,257 402 Marked 36 2 Unmarked 3,221 400 4) EP-Temporalf Deshka weir Julian day 185-216 217-228 229-245 Inspectede 804 970 1,482 Marked 10 6 20 Unmarked 747 1,012 1,462 5) EP-Temporalg Montana weir a CM=”Complete Mixing Test” and EP=”Equal Proportions Test” (see text; Arnason et al. 1996) b χ2 = 26.51, P =<0.001. c χ2 = 2.98, P =0.225. d χ2 =0.763, P = 0.382. e Number of fish inspected for marks is estimated. f χ2 = 3.05,P=0.217 g Insufficient recaptures to conduct test (2 recaptures at Montana weir in size class 40-55 cm). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 17 October 2015 Table I-8. Diagnostic tests for mark-recapture data for mainstem Susitna River Coho Salmon >55 cm METF, 2014a. Test 1) CM-Spatialb Gear Fishwheel 1 west Fishwheel 2 east Marks 95 93 Recaptured 25 9 Not recaptured 70 84 2) CM-Temporalc Julian day 188-207 208-212 213-232 Marks 70 52 66 Recaptured 17 10 7 Not recaptured 53 42 59 3) EP-Spatiald Weir site Deshka R Montana Cr Inspectede 8,288 529 Marked 32 2 Unmarked 8,256 527 4) EP-Temporalf Deshka weir Julian day 185-216 217-228 229-245 Inspectede 1,817 2,505 3,998 Marked 5 6 21 Unmarked 1,812 2,499 3,976 5) EP-Temporalg Montana weir a CM=”Complete Mixing Test” and EP=”Equal Proportions Test” (see text; Arnason et al. 1996) b χ2 = 7.69, P =0.006 c χ2 = 4.35, P =0.113 d χ2 ~0, P ~ 1 e Number of fish inspected for marks is estimated. f χ2 = 4.01, P = 0.13 g Insufficient recaptures to conduct test (2 recaptures at Montana weir in size class >55cm). STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 18 October 2015 Table I-9. Estimated abundance, number of radio tags deployed, and relative weights (number of spawners per tag) used to estimate abundance within size stratum for Coho Salmon spawning upstream from the lower mainstem tagging site in the Susitna River, 2014. Size Strata Estimated Abundance Estimated SE Radio Tags Deployeda Relative Weight spawners/tag 40-55 cm METF 37,069 6,495 394 94 ≥55 cm METF 47,810 7,667 188 254 > 40 cm METF 84,879 9,550 a Available to estimate spawning distribution Table I-10. Coho Salmon spawning distributions, based on weighted abundance (Table I-3), in the mainstem Susitna River above the lower river tagging site, 2014. Estimated Abundance Intervals Location SE 95% lower 95% upper Susitna River above the mainstem tagging site 84,879 9,550 68,799 106,083 PRM 34–102.4 mainstem Susitna River a 10,889 2,096 7,792 15,979 Deshka River 15,377 1,138 13,737 18,215 Eastside Susitna River 16,515 2,790 12,446 23,312 Talkeetna River 12,130 2,244 8,797 17,598 PRM 102.4–153.4 mainstem Susitna River b 6,184 1,414 4,030 9,559 Chulitna River 23,783 3,788 18,307 33,099 a PRM 34 upstream to the Chulitna River Confluence b Chulitna River Confluence to Devils Canyon STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 1 October 2015 Figure I-1. Empirical cumulative distribution functions (ECDF) of length (in mm) of Chinook Salmon (METF ≥ 50 cm) marked during first event sampling at the lower mainstem Susitna River tagging site and all recaptures during second event sampling at the Deshka River and Montana Creek weirs, 2014. 500 600 700 800 900 1000 0.0 0.2 0.4 0.6 0.8 1.0 Recaptures - Marks : Pooled Deshka-Montana Length mmFn(x)Marked Recaptured D_max= 0.145 D_max at 770 mm p-value= 0.016 Obs in Marked = 494 Obs in Recaptured = 138 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 2 October 2015 Figure I-2. Empirical cumulative distribution functions (ECDF) of length (in millimeters, mm) of Chinook (METF ≥ 500 mm) inspected for marks and all recaptured salmon during second event sampling at the Deshka River weir, 2014. 500 600 700 800 900 0.0 0.2 0.4 0.6 0.8 1.0 Recaptures - Captures : Deshka Length mmFn(x)Captured Recaptured D_max= 0.21 D_max at 785 mm p-value= 0 Obs in Captured = 251 Obs in Recaptured = 124 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 3 October 2015 Figure I-3. Empirical cumulative distribution functions (ECDF) of length (in mm) of Chinook Salmon (METF ≥ 500 mm) inspected for marks and all recaptured salmon during second event sampling at the Montana Creek weir, 2014. 500 600 700 800 900 0.0 0.2 0.4 0.6 0.8 1.0 Recaptures - Captures : Montana Length mmFn(x)Captured Recaptured D_max= 0.372 D_max at 590 mm p-value= 0.026 Obs in Captured = 226 Obs in Recaptured = 14 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 4 October 2015 Figure I-4. The 4-day lag between total weir count and radio-tagged Chinook Salmon at the Deshka River weir, 2014. 246810DESHKA DAYRadiotagged05/28 06/02 06/07 06/12 06/17 06/22 06/27 07/02 07/07 07/12 07/17 07/22 07/27 04008001200DAYWeir Count05/28 06/02 06/07 06/12 06/17 06/22 06/27 07/02 07/07 07/12 07/17 07/22 07/27 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 5 October 2015 Figure I-5. Empirical cumulative distribution functions of METF length (mm) of all Chinook Salmon (> 500 mm) marked during first event at the lower Yentna River tagging site at RM 6 and of all salmon recaptured during second event sampling at RM 18 of the lower Yentna River, 2014. 500 600 700 800 900 1000 1100 0.0 0.2 0.4 0.6 0.8 1.0 Recaptures - Marks : Pooled Fishwheel and Gillnet Length mmFn(x)Marked Recaptured D_max= 0.088 D_max at 640 mm p-value= 0.654 Obs in Marked = 1277 Obs in Recaptured = 59 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 6 October 2015 Figure I-6. Empirical cumulative distribution functions of METF length (mm) of Chinook Salmon (> 500 mm) inspected for marks during second event sampling at the Yentna RM 18 east fishwheel, and all salmon recaptured during inspection in 2014. 500 600 700 800 900 0.0 0.2 0.4 0.6 0.8 1.0 Recaptures - Captures : East Wheel Length mmFn(x)Captured Recaptured D_max= 0.196 D_max at 610 mm p-value= 0.122 Obs in Captured = 719 Obs in Recaptured = 31 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 7 October 2015 Figure I-7. Empirical cumulative distribution functions of METF length (mm) of Chinook Salmon (>500 mm) inspected for marks during second event sampling at the Yentna RM 18 west fishwheel, and all salmon recaptured during inspection in 2014. 500 600 700 800 900 1000 1100 0.0 0.2 0.4 0.6 0.8 1.0 Recaptures - Captures : West Wheel Length mmFn(x)Captured Recaptured D_max= 0.131 D_max at 625 mm p-value= 0.666 Obs in Captured = 536 Obs in Recaptured = 25 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 8 October 2015 Figure I-8. Empirical cumulative distribution functions of METF length (mm) of Chinook Salmon (>500 mm) inspected for marks during second event sampling (pooled data) and all salmon recaptured during inspection at Yentna RM 18 in 2014. 500 600 700 800 900 1000 1100 0.0 0.2 0.4 0.6 0.8 1.0 Recaptures - Captures : Pooled Data Length mmFn(x)Captured Recaptured D_max= 0.053 D_max at 550 mm p-value= 0.974 Obs in Captured = 1255 Obs in Recaptured = 59 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 9 October 2015 Figure I-9. Timing at Yentna River second event fishwheels (RM 18) of all fish caught >500 mm METF and of recaptures. Catch >=500mm Recapture DayFrequency140150160 170 180 1900100200300400 Recaptures Recapture DayFrequency140150160 170 180 190051015 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 10 October 2015 Figure I-10. Empirical cumulative distribution functions of METF length (mm) of all Coho Salmon (> 400 mm) marked during first event at the lower mainstem Susitna River tagging site and of all salmon recaptured during second event sampling at the Deshka River and Montana Creek weirs during second event, 2014. 400 450 500 550 600 0.0 0.2 0.4 0.6 0.8 1.0 Recaptures - Marks : Pooled Wheels ( 400 - 1000 mm) Length mmFn(x)Marked Recaptured D_max= 0.247 D_max at 525 mm p-value= 0.001 Obs in Marked = 582 Obs in Recaptured = 72 STUDY COMPLETION REPORT SALMON ESCAPEMENT STUDY (9.7) Susitna-Watana Hydroelectric Project Alaska Energy Authority FERC Project No. 14241 Appendix I – Page 11 October 2015 Figure I-11. Empirical cumulative distribution functions of METF length (mm) of Coho Salmon (> 400 mm) inspected for marks during second event sampling at the Deshka River weir, 2014 and of all salmon recaptured during inspection. 400 450 500 550 600 650 0.0 0.2 0.4 0.6 0.8 1.0 Recaptures - Captures : Deshka ( 400 - 1000 mm) Length mmFn(x)Captured Recaptured D_max= 0.286 D_max at 565 mm p-value= 0 Obs in Captured = 304 Obs in Recaptured = 68